Article
Biotechnology & Applied Microbiology
Dong Zhang, Yifan Wu, Zonghuan Li, Hairen Chen, Siyuan Huang, Chao Jian, Aixi Yu
Summary: The diabetic milieu negatively impacts macrophage-derived exosomes, leading to reduced osteogenic potential and fracture repair ability. High levels of miR-144-5p in diabetic bone marrow-derived exosomes target Smad1 to affect bone regeneration, which can be reversed by miR-144-5p inhibition.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Orthopedics
Zhen Guo, Jing Li, Jiyong Tan, Sainan Sun, Qing Yan, Hao Qin
Summary: Osteoporosis is a common bone disease that increases the risk of fracture. Through experiments, it has been found that exosomes secreted by senescent osteoblasts can accelerate endothelial cell senescence through the miR-214-3p/L1CAM pathway. This study reveals the role of exosomes in the skeletal microenvironment.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2023)
Article
Cell Biology
Kewei Yu, Niveditha Ramkumar, Kenneth Kin Lam Wong, Gritta Tettweiler, Esther M. Verheyen
Summary: Homeodomain-interacting protein kinases (Hipks) regulate cell proliferation, apoptosis, and tissue development. Depletion of Salt-inducible kinases Sik2 or Sik3 can suppress Hipk-induced overgrowth, while co-expression of constitutively active forms of Sik2 or Sik3 with Hipk can promote tumorous phenotypes. Our study suggests that Sik proteins may regulate Hipk protein stability and/or activity, and their interaction could lead to post-translational modification of Hipk.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yi-Yung Hung, Chen-Kai Chou, Yi-Chien Yang, Hung-Chun Fu, El-Wui Loh, Hong-Yo Kang
Summary: This study found that in patients with major depressive disorder, the expression levels of miR-146a in exosomes were higher, and patients who achieved remission showed lower levels of various microRNAs before treatment and increased levels after treatment. Let-7e, miR-145, and miR-146a showed acceptable discrimination between the remission and non-remission groups.
Article
Engineering, Environmental
Lang Chen, Yuan Xiong, Yiqiang Hu, Chenyan Yu, Adriana C. Panayi, Wu Zhou, Faqi Cao, Yun Sun, Mengfei Liu, Guodong Liu, Hang Xue, Liangcong Hu, Bobin Mi, Guohui Liu
Summary: This study demonstrates that Treg-derived exosomes containing miR-142-3p can promote osteogenesis and angiogenesis in bone mesenchymal stem cells and human umbilical vein endothelial cells. Both Treg-derived exosomes and miR-142-3p show promising potential as therapeutic agents for bone remodeling, with TGFBR1/SMAD2 identified as being involved in the promotion of bone healing by Treg-derived exosomes.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Neurosciences
Kanako Iwasaki, Tomoyuki Fujiyama, Shinya Nakata, Minjeong Park, Chika Miyoshi, Noriko Hotta-Hirashima, Aya Ikkyu, Miyo Kakizaki, Fumihiro Sugiyama, Seiya Mizuno, Manabu Abe, Kenji Sakimura, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
Summary: Sleep is regulated in a homeostatic manner and deprivation increases sleep need. A recent study found a mutation that leads to increased sleep need in mice. Neuron-specific expression plays a role in increasing sleep in experimental mice.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Pharmacology & Pharmacy
Z-W Yan, P. Du, W. Zhang, W-C Sun, L. Jia, M. Jiang, Y. Liu, H-L Guo
Summary: MiR-182 is elevated in patients with fracture of tibial plateau, inhibiting proliferation and differentiation of osteoblasts, affecting fracture healing. Knockdown of miR-182 might be a novel therapeutic approach for fracture healing.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2021)
Article
Immunology
Shouming Cao, Haiying Wu, Yan Niu, Lu Wang, Fengjun Wang
Summary: This study found that serum exosome-derived miRNAs have the potential to predict the early effectiveness of SLIT in AR patients. Among them, has-miR-24-3p and has-miR-128-3p showed decreased expression in the effective group, while has-miR-206 showed increased expression, indicating potential as biomarkers for predicting the early efficacy of SLIT.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jiung-Pang Huang, Chih-Chun Chang, Chao-Yu Kuo, Kuang-Jing Huang, Etienne M. Sokal, Kuan-Hsing Chen, Li-Man Hung
Summary: EXO-miRNAs have the potential to be used as biomarkers for diabetic HFpEF. The study found that miR-30d-5p and miR-126a-5p were associated with HFpEF, and may be important for the development of diagnostic tools for diabetic HFpEF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Cai Chen, Ping Zeng, Jinfu Liu, Xiangbin Rong, Zhao Tian, Qiang Din, Hongcheng Tao
Summary: The research discovered that Panax Notoginseng Saponins inhibited the differentiation of RAW264.7 cells induced by RANKL, and in the exosome intervention environment, PNS reversed the osteogenic differentiation inhibition of MC3T3-E1 cells by OC-Exos.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Cell & Tissue Engineering
Jianghu Huang, Feiyue Lin, Chuncai Xu, Yang Xu
Summary: The study found that LINC00662 promotes osteosarcoma progression via the miR-103a-3p/SIK2 axis. Downregulation of LINC00662 affects malignant behaviors and tumor growth in OS cells.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Oncology
Jilong Zou, Jiabing Sun, Hongjun Chen, Xinming Fan, Zhenrui Qiu, Yuan Li, Jianhui Shi
Summary: The study investigated the role of miR-26a in osteoblasts and fractures, showing that overexpression of miR-26a could significantly increase cell viability and proliferation of osteoblasts, reduce cell death, promote osteoblastic activity, and enhance mineralized nodule formation. Upregulation of miR-26a negatively regulated the expression of PTEN, suggesting that miR-26a may be a potential therapeutic target for bone regeneration and fracture treatment.
ANNALS OF TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Zhenchu Tang, Shenglan Hu, Ziwei Wu, Miao He
Summary: This study evaluated the value of exosome-based microRNA therapy in SCA3 and demonstrated the therapeutic effects of intravenously administrated ATXN3 targeting microRNAs in transgenic SCA3 mouse models. The targeted delivery of miR-25 and miR-181a by modified exosomes successfully reduced mutant ATXN3 expression and improved motor abilities in SCA3 mice without increasing neuroinflammatory response.
MOLECULAR MEDICINE
(2023)
Article
Cell Biology
Shilan Shafei, Mehdi Khanmohammadi, Hossein Ghanbari, Vajihe Taghdiri Nooshabadi, Seyed Hossein Ahmadi Tafti, Sharam Rabbani, Maniya Kasaiyan, Mohsen Basiri, Gholamreza Tavoosidana
Summary: This study demonstrated that combination treatment of microRNA-126 and microRNA-146a mimics in exosomes is desirable for cardiac regeneration after myocardial infarction. Exosomes loaded with microRNAs showed a synergistic impact on cell migration and tube formation, and encapsulating them in alginate hydrogel could reduce infarct size and improve angiogenesis in myocardial infarction.
CELL AND TISSUE RESEARCH
(2022)
Article
Cell Biology
Peng Zhao, Jianhua Cheng, Bin Li, Ding Nie, Chuzhong Li, Songbai Gui, Hongyun Wang, Yazhuo Zhang
Summary: This study demonstrated that miR-149 and miR-99a-3p induced by exosomes could suppress the progression of IPA. The miRNAs were significantly inhibited in pituitary adenoma cells and tissues, and played inhibitory roles in cell proliferation, metastasis, and angiogenesis.
CELL BIOLOGY AND TOXICOLOGY
(2021)
Article
Engineering, Environmental
Pei Liu, Yuan Xiong, Lang Chen, Chuanchuan Lin, Yayan Yang, Ze Lin, Yongsheng Yu, Bobin Mi, Guohui Liu, Xiufeng Xiao, Qian Feng
Summary: Diabetic wound, a common complication of diabetes, is a challenging condition to treat. In this study, a multifunctional hydrogel was designed as a diabetic wound dressing, which effectively stopped bleeding, provided optimal healing conditions, and promoted angiogenesis for accelerated wound healing.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Bobin Mi, Lang Chen, Yuan Xiong, Yayan Yang, Adriana C. Panayi, Hang Xue, Yiqiang Hu, Chenchen Yan, Liangcong Hu, Xudong Xie, Ze Lin, Wu Zhou, Faqi Cao, Xiufeng Xiao, Qian Feng, Guohui Liu
Summary: The ratios of osteoblast/osteoclast and M1/M2 macrophages are crucial for fracture healing. This study developed a cocktail therapy to simultaneously regulate the balance of these cells, leading to enhanced fracture repair. Each component of the cocktail therapy was found to be indispensable, providing insight into synergistic strategies for fracture repair.
Article
Engineering, Biomedical
Lang Chen, Chenyan Yu, Yuan Xiong, Kai Chen, Pei Liu, Adriana C. Panayi, Xiufeng Xiao, Qian Feng, Bobin Mi, Guohui Liu
Summary: Researchers developed an injectable, self-healing, adhesive hydrogel that combines Stromal Cell-Derived Factor-1 alpha (SDF-1 alpha) and M2 macrophage derived exosomes (M2D-Exos). This hydrogel enhanced proliferation and migration of human bone marrow mesenchymal stem cells and human umbilical vein endothelial cells, promoting osteogenesis and angiogenesis both in vivo and in vitro. It provides a new modality for accelerating bone repair by coupling osteogenesis, angiogenesis, and resisting infection at all stages.
BIOACTIVE MATERIALS
(2023)
Review
Pharmacology & Pharmacy
Li Lu, Yuan Xiong, Ze Lin, Xiangyu Chu, Adriana C. Panayi, Yiqiang Hu, Juan Zhou, Bobin Mi, Guohui Liu
Summary: This review summarizes the multiple effects of kinsenoside (KD), including anti-inflammatory and anti-oxidative abilities, as well as protection for liver, blood vessels, and immune system. The article also discusses the physicochemical properties, pharmacokinetic profiles, and advances in cultivation and drug delivery systems of KD.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Surgery
Hang Xue, Wu Zhou, Zhenhe Zhang, Adriana C. Panayi, Yuan Xiong, Shuhua Yang, Bobin Mi, Guohui Liu, Xianzhe Liu
Summary: Primary mucinous lung adenocarcinoma is an extremely rare subtype of lung adenocarcinoma that is difficult to diagnose and often misdiagnosed. This case report provides valuable insights into bone metastasis cases with atypical symptoms, aiming to assist clinicians in making timely and accurate diagnoses.
FRONTIERS IN SURGERY
(2022)
Article
Surgery
Liangcong Hu, Xudong Xie, Tiantian Wang, Bobin Mi, Hang Xue, Ze Lin, Yuan Xiong, Yiqiang Hu, Wu Zhou, Faqi Cao, Guohui Liu
Summary: This study evaluated the effect of traction-bed-assisted reduction and double-plate internal fixation in the treatment of comminuted and coronally split intertrochanteric femoral fracture. The experimental group showed shorter reduction and operation time, as well as better post-operative hip joint function. However, there were no significant differences between the two groups in other indicators.
FRONTIERS IN SURGERY
(2022)
Article
Chemistry, Multidisciplinary
Lang Chen, Chenyan Yu, Wanting Xu, Yuan Xiong, Peng Cheng, Ze Lin, Zhenhe Zhang, Leonard Knoedler, Adriana C. Panayi, Samuel Knoedler, Junqing Wang, Bobin Mi, Guohui Liu
Summary: Ongoing research has shown that the interaction between macrophages and mesenchymal stem cells (MSCs) is significant. Lysine-specific demethylase 6B (KDM6B) has been found to control the osteogenic differentiation of MSCs. However, the role of KDM6B in bone marrow-derived macrophages (BMDMs) is still controversial. This study demonstrates that KDM6B serves as a positive regulator for osteogenic differentiation by decreasing the M1/M2 ratio both in vitro and in vivo.
Review
Chemistry, Multidisciplinary
Yuan Xiong, Qian Feng, Li Lu, Kangkang Zha, Tao Yu, Ze Lin, Yiqiang Hu, Adriana C. C. Panayi, Vahideh Nosrati-Ziahmagi, Xiangyu Chu, Lang Chen, Mohammad-Ali Shahbazi, Bobin Mi, Guohui Liu
Summary: Diabetic foot ulcer (DFU) is a common complication of diabetes, and the lack of efficient therapy poses challenges for patients. Immunomodulatory hydrogels offer potential solutions for DFU treatment. This review comprehensively discusses recent advances in the fabrication and regenerative use of hydrogels for altering the immune microenvironment of DFUs. It also explores the potential of hydrogels for multimodal therapy and improved efficacy and safety.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Biotechnology & Applied Microbiology
Bin Lv, Juntao Wu, Yuan Xiong, Xudong Xie, Ze Lin, Bobin Mi, Guohui Liu
Summary: Bone regeneration is a pathophysiological process that requires the involvement of multiple systems, and the immune system plays a crucial role in it. Researchers have applied various therapeutic strategies, such as intervention of surface characteristics or local delivery systems, to create an ideal microenvironment for bone tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yuan Xiong, Ze Lin, Pengzhen Bu, Tao Yu, Yori Endo, Wu Zhou, Yun Sun, Faqi Cao, Guandong Dai, Yiqiang Hu, Li Lu, Lang Chen, Peng Cheng, Kangkang Zha, Mohammad-Ali Shahbazi, Qian Feng, Bobin Mi, Guohui Liu
Summary: Diabetic wound therapy is a significant challenge in medicine. A whole-course-repair system using a hydrogel has been introduced to achieve concurrent neurogenesis and angiogenesis for accelerated wound healing. The hydrogel can be injected into wounds to provide a physical barrier and release magnesium ions and engineered small extracellular vesicles for synergistic effects. This system recruits mesenchymal stem cells and stimulates neurogenic differentiation, while also promoting angiogenesis at the wound site. It offers a novel platform for combined diabetic wound therapy.
ADVANCED MATERIALS
(2023)
Review
Medicine, General & Internal
Shuai Liu, Jiang-Ming Yu, Yan-Chang Gan, Xiao-Zhong Qiu, Zhe-Chen Gao, Huan Wang, Shi-Xuan Chen, Yuan Xiong, Guo-Hui Liu, Si-En Lin, Alec McCarthy, Johnson V. John, Dai-Xu Wei, Hong-Hao Hou
Summary: Biomimetic materials based on natural biomaterials offer cells biochemical and biophysical cues that mimic the in vivo extracellular matrix, making them ideal for tissue engineering and regenerative medicine. This paper provides an overview of recent progress in biomimetic natural biomaterials (BNBMs), including their preparation, functionality, potential applications, and future challenges. The paper also highlights recent advances in the fabrication and functionalization of BNBMs, as well as their applications in tissue engineering.
MILITARY MEDICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Ze Lin, Yuan Xiong, Yun Sun, Ruiyin Zeng, Hang Xue, Yiqiang Hu, Lang Chen, Guodong Liu, Adriana C. Panayi, Wu Zhou, Faqi Cao, Fei Gao, Bobin Mi, Guohui Liu
Summary: Extracellular vesicles enriched with miRNA-21-5 p, released after traumatic brain injury (TBI), can promote faster healing of fractures by enhancing bone-forming cells differentiation and repair. This study provides new insights into the immune mechanisms and regulation of fracture healing in the context of TBI.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Review
Cell & Tissue Engineering
Ranyang Tao, Bobin Mi, Yiqiang Hu, Sien Lin, Yuan Xiong, Xuan Lu, Adriana C. C. Panayi, Gang Li, Guohui Liu
Summary: This review outlines the role of the peripheral nervous system (PNS) in bone regeneration, including the conserved role of nerves in tissue regeneration across different species and the distribution of the PNS in the skeletal system. It summarizes the communication between the PNS and bone-lineage cells, vasculature, and immune cells in the bone microenvironment. The review concludes that the PNS regulates bone regeneration through neuropeptides or neurotransmitters and cells in the peripheral nerves, and understanding this role can inform the development of strategies for bone repair and regeneration based on bone-nerve crosstalk.
Review
Medicine, General & Internal
Yuan Xiong, Bo-Bin Mi, Ze Lin, Yi-Qiang Hu, Le Yu, Kang-Kang Zha, Adriana C. Panayi, Tao Yu, Lang Chen, Zhen-Ping Liu, Anish Patel, Qian Feng, Shuan-Hu Zhou, Guo-Hui Liu
Summary: This article reviews the regulatory role of the immune microenvironment in bone, cartilage, and soft tissue repair and regeneration, and introduces novel strategies for regulating the healing process. A comprehensive understanding of the interaction between immune cells and functional tissue cells is valuable for enhancing the therapeutic effect of bone, cartilage, and soft tissue repair and regeneration through regulating the immune microenvironment.
MILITARY MEDICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Liangcong Hu, Xudong Xie, Hang Xue, Tiantian Wang, Adriana C. Panayi, Ze Lin, Yuan Xiong, Faqi Cao, Chengcheng Yan, Lang Chen, Peng Cheng, Kangkang Zha, Yun Sun, Guodong Liu, Chenyan Yu, Yiqiang Hu, Ranyang Tao, Wu Zhou, Bobin Mi, Guohui Liu
Summary: miR-1224-5p is a key regulator of bone osteogenesis, which is associated with fracture healing and osteoporosis development. By targeting ADCY2 and the Rap1 signaling pathway, miR-1224-5p can slow down osteoclast differentiation and promote osteoblast differentiation. In vivo experiments showed that overexpression of miR-1224-5p can promote fracture healing and ameliorate the progression of osteoporosis.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
