期刊
FRONTIERS IN PLANT SCIENCE
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2020.00518
关键词
meiosis; sterile; OsMFS1; OsHOP2; rice
资金
- National Key Research and Development Program of China [2016YFD0101107, 2016YFD0101801]
- National Transform Science and Technology Program [2016ZX08001004-002]
- National Nature Science Foundation of China [31701402]
- Key Laboratory of Biology, Genetics and Breeding of Japonica Rice in the Mid-lower Yangtze River
- Ministry of Agriculture, China, Jiangsu Plant Gene Engineering Research Center
- Jiangsu Collaborative Innovation Center for Modern Crop Production
Meiosis plays an essential role in the production of gametes and genetic diversity of posterities. The normal double-strand break (DSB) repair is vital to homologous recombination (HR) and occurrence of DNA fragment exchange, but the underlying molecular mechanism remain elusive. Here, we characterized a completely sterile Osmfs1 (male and female sterility 1) mutant which has its pollen and embryo sacs both aborted at the reproductive stage due to severe chromosome defection. Map-based cloning revealed that the OsMFS1 encodes a meiotic coiled-coil protein, and it is responsible for DSB repairing that acts as an important cofactor to stimulate the single strand invasion. Expression pattern analyses showed the OsMFS1 was preferentially expressed in meiosis stage. Subcellular localization analysis of OsMFS1 revealed its association with the nucleus exclusively. In addition, a yeast two-hybrid (Y2H) and pull-down assay showed that OsMFS1 could physically interact with OsHOP2 protein to form a stable complex to ensure faithful homologous recombination. Taken together, our results indicated that OsMFS1 is indispensable to the normal development of anther and embryo sacs in rice.
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