Article
Food Science & Technology
Babak Pakbin, Wolfram Manuel Bruck, Samaneh Allahyari, John W. A. Rossen, Razzagh Mahmoudi
Summary: This study investigated the prevalence, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of C. sakazakii strains isolated from powdered infant formula milk (PIFM) samples collected in Tehran, Iran. The results showed a high prevalence rate of C. sakazakii in PIFM samples, with high resistance to multiple antibiotics and a diverse genetic profile. The study highlights the need for novel microbial surveillance systems to control the contamination of this foodborne pathogen in infant foods.
Article
Agriculture, Dairy & Animal Science
Ju Liu, Guoyang Xie, Qin Xiong, Dan Mu, Hengyi Xu
Summary: A simple and sensitive fluorescent turn-off aptasensor was developed for detecting viable Cronobacter sakazakii in powdered infant formula. The sensor showed good performance and specificity in detecting the pathogen within 3 hours in both pure culture and formula samples.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Yuan-song Zhang, Xuechao Xu, Juanli Yang, Mengyuan Tan, Wenyuan Zhou, Lu Gao, Zhen-quan Yang
Summary: In this study, a chromogenic system based on phage-directed immobilized nanozyme was developed for colorimetric measurement of Cronobacter sakazakii in powdered infant formula (PIF). The method showed high specificity and low detection limit, successfully detecting C. sakazakii and achieving good recovery rates.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Lihan Wang, Xinyi Pang, Jiayuan Zhao, Haonan Jin, Xinyan Yang, Shiqian Fu, Shasha Cheng, Hongxuan Li, Chao Miao, Chaoxin Man, Yujun Jiang
Summary: JK004 is a novel virulent phage isolated from the native environment of C. sakazakii, known for its tolerance to heat, pH, and osmotic-stress. It shows excellent antibacterial activity against C. sakazakii and biofilms, without containing any known virulence, transduction, or antibiotic resistance genes, indicating its safety for practical applications in food production. The study suggests the potential of JK004 as an effective strategy for controlling C. sakazakii.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Geng Zou, Libere Ndayishimiye, Lingxiang Xin, Manshan Cai, Longjian Zhang, Jie Li, Zhiyong Song, Renwei Wu, Yang Zhou, Yuanguo Shi, Yingwang Ye, Rui Zhou, Jinquan Li
Summary: In this study, a phage termed LPCS28 with a broad lysis spectrum against C. sakazakii was isolated from environmental water samples. LPCS28 showed high thermal stability, with no significant decrease in titer after heating at 60 degrees C for one hour. Upon thermal processing at 63 degrees C for 30 min, LPCS28 effectively inhibited C. sakazakii in reconstituted powdered infant formula and liquid milk, reducing its concentration to below the limit of detection within 9 h at 37 degrees C. Phylogenetic analysis classified LPCS28 as a new genus, Nanhuvirus, within the family Straboviridae. These results highlight the potential of LPCS28 as a biological control agent against pathogenic C. sakazakii in the dairy industry.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Yanpeng Yang, Sheng Ma, Kailun Guo, Du Guo, Jiahui Li, Muxue Wang, Yutang Wang, Chunling Zhang, Xiaodong Xia, Chao Shi
Summary: This study found that combined LED illumination and citral treatment can effectively reduce the abundance of C. sakazakii in reconstituted powdered infant formula. The combination treatment also destroys cellular morphology and membrane integrity, and induces lipid peroxidation. Neither LED illumination nor citral treatment results in DNA breakdown in C. sakazakii.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Zhanwen Zheng, Yawen Xie, Sheng Ma, Junhong Tu, Jiahui Li, Sen Liang, Yunfeng Xu, Chao Shi
Summary: The study demonstrated that 405-nm LED light effectively inactivated C. sakazakii in PIF, reducing its tolerance to various environmental stressors. LED illumination also downregulated the transcription levels of tolerance-associated genes and caused damage to bacterial cell membranes. This suggests the potential of 405-nm LED technology in preventing and controlling pathogens in food processing and storage environments.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Jae-Ik Lee, Sang-Soon Kim, Dong-Hyun Kang
Summary: The aim of this study was to develop a new primer-probe set for detecting Cronobacter sakazakii based on comparative genomics. The sensitivity and selectivity of the designed primer were confirmed, and it was found to have minimal interference from other substances. Additionally, the detection efficiency of the designed primer in various food samples was comparable to that of conventionally used selective media.
Article
Food Science & Technology
Shijue Gao, Chongzhen Sun, Hanlu Hong, Ravi Gooneratne, Anthony Mutukumira, Xiyang Wu
Summary: In this study, a novel detection method was developed to effectively differentiate viable from dead bacteria, particularly in the detection of Cronobacter sakazakii in food samples. The method demonstrated faster and more accurate results compared to traditional culture-based methods, making it suitable for detecting complex food samples.
Article
Microbiology
Xin Gan, Menghan Li, Shaofei Yan, Xiaofei Wang, Wei Wang, Fengqin Li
Summary: The study found that Cronobacter sakazakii from powdered infant formula manufacturing factories in China are mostly pan-susceptible with weak biofilm-forming abilities. The isolates, mostly ST64, exhibited clinical relevance and shared a close genetic background, indicating possible cross-contamination events between processing rooms and external environments.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Food Science & Technology
Rajni Chauhan, Ben D. Tall, Gopal Gopinath, Wamik Azmi, Gunjan Goel
Summary: This article reviews the factors and strategies responsible for the survival and persistence of Cronobacter sakazakii in powdered infant formula (PIF), focusing on thermal resistance, antibiotic or antimicrobial resistance, virulence potential, and stress-associated gene expression. This pathogen poses a high risk to neonates and its ability to survive in low-moisture foods is influenced by intrinsic and extrinsic factors.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Chemistry, Analytical
Bao Gao, Tongtong Ma, Lin Feng, Xiaolin Huang, Xuelan Chen, Yonghua Xiong
Summary: In this study, a novel immunosensor using the light scattering intensity of silver and gold alloy as a signal transducer was developed to enhance the sensitivity and linearity for quantitative detection of Cronobacter muytjensii. The developed method showed high sensitivity with a low detection limit, wide dynamic range, and acceptable accuracy for quantitative detection of C. muytjensii in real PIF samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Infectious Diseases
Mohamed E. El Zowalaty, Linda Falgenhauer, Stephen Forsythe
Summary: The study presents the first draft genome sequence of C. sakazakii strain MEZCS99 isolated from a healthy chicken in South Africa, providing insights into its genetic characteristics and serving as a reference point for future molecular epidemiological studies on livestock-associated C. sakazakii.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Agriculture, Dairy & Animal Science
Shiqian Fu, Xue Qin, Zhenghui Wang, Xinyan Yang, Sihan Chen, Tao Yang, Haonan Jin, Chaoxin Man, Yujun Jiang
Summary: This study utilized whole genome sequence analysis to identify unique DNA fragments of C. sakazakii, designing specific and stable primers for detection using LAMP and PCR techniques. By combining LAMP with LFD, a rapid and highly specific method for detecting C. sakazakii was developed, showing potential for use in detecting the pathogen in powdered infant formula.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Immunology
Margaret G. Taylor, Megan H. Amerson-Brown, Kristina Hulten, Lindsay H. Cameron, Galit Holzmann-Pazgal, Morven S. Edwards, Catherine E. Foster
Summary: This study reports two cases of infants hospitalized with Cronobacter sakazakii meningitis, both of whom had exposure to powdered infant formula at home. Early advanced brain imaging is recommended, and reporting to public health officials may help identify outbreaks.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2021)
Article
Pediatrics
Khalid I. Elsayh, Khaled Saad, Helal F. Hetta, Mervat A. M. Youssef, Mostafa M. Embaby, Ismail L. Mohamed, Safwat M. Abdel-Aziz, Zeinab Albadry M. Zahran, Amira Elhoufey, Aliaa M. A. Ghandour, Asmaa M. Zahran
Summary: Hydroxyurea treatment can restore the immune parameters in children with sickle cell anemia, including increased CD3+, CD4+, Th1, and CD8+ T cells and decreased NK, Th2, and Tc2 cells, resulting in an increase in the Th1/Th2 and Tc1/Tc2 ratios.
PEDIATRIC RESEARCH
(2023)
Article
Rheumatology
S. Salah, H. Lotfy, M. H. Abu-Zaid, M. El Gaafary, H. Abdulhady, H. Salah, E. Abd El-Latif, Y. Farag, M. Eissa, S. Esam Maher, A. Radwan, Amira T. El-Shanawany, B. M. Medhat, D. El Mikkawy, D. M. Mosa, G. El Deriny, M. Mortada, N. S. Osman, N. A. Fouad, N. E. Elkaraly, S. S. Mohamed, S. A. Tabra, W. A. Hassan, Y. Amer, S. I. Nasef, Y. El Miedany
Summary: This study used the Delphi technique to establish a consensus among experts on the treat-to-target management of FMF in children. A total of 12 management recommendations were obtained, along with developed management algorithms. The study provides comprehensive treatment strategies for healthcare professionals involved in FMF management.
EGYPTIAN RHEUMATOLOGY AND REHABILITATION
(2022)
Article
Infectious Diseases
Azza Abdelaal, Ahmed Abu-Elfatth, Lamees M. Bakkar, Hanan G. Abd El-Azeem, Helal F. Hetta, Eman R. Badawy
Summary: This study evaluated coagulopathy and multiple hemostatic markers in COVID-19 patients and found that this could be associated with poor outcomes. d-dimer is a key tool in diagnosis and severity evaluation. Early screening and proper management of coagulation abnormalities in COVID-19 patients can improve outcomes.
Review
Biotechnology & Applied Microbiology
Hayder M. M. Al-Kuraishy, Ali I. I. Al-Gareeb, Helal F. F. Hetta, Athanasios Alexiou, Marios Papadakis, Gaber El-Saber Batiha
Summary: Monkeypox is a zoonotic disease caused by the Monkeypox virus. The clinical manifestations of human Monkeypox are similar to smallpox, but with the distinguishing sign of early lymphadenopathy. The importance of Monkeypox needs further research to understand its pathogenesis and management.
Review
Biotechnology & Applied Microbiology
Hayder M. Al-Kuraishy, Ali I. Al-Gareeb, Helal F. Hetta, Athanasios Alexiou, Marios Papadakis, Gaber El-Saber Batiha
Summary: Heparanase (HPSE) is an enzyme that cleaves heparan sulfate (HS), contributing to extracellular matrix degradation and remodeling. HPSE-induced cleavage of HS promotes autophagy and release of growth factors. The interaction between HPSE and HS triggers the release of chemokines and cytokines, leading to inflammatory response, cytokine storm, and coagulopathy. Targeting HPSE with specific inhibitors may reduce complications in SARS-CoV-2 and MPXV infections.
Article
Agriculture, Dairy & Animal Science
Helmy A. A. Torky, Hebatallah M. Saad, Samy A. Khaliel, Asmaa T. Kassih, Jean-Marc Sabatier, Gaber El-Saber Batiha, Helal F. Hetta, Eman M. Elghazaly, Michel De Waard
Summary: Corynebacterium pseudotuberculosis (C. pseudotuberculosis) is a causative agent of chronic diseases in animals and humans, with poor therapeutic response. A study analyzed 120 samples and found only 10% positive for C. pseudotuberculosis. Genetic analysis showed intra-subtyping variation. The study also identified immune expression patterns in affected lymph nodes, indicating the involvement of the NF-kappa B/p65 signaling pathway in the inflammatory response to control the pathogen.
Review
Biochemistry & Molecular Biology
Helal F. Hetta, Yasmin N. Ramadan, Alhanouf I. Al-Harbi, Esraa A. Ahmed, Basem Battah, Noura H. Abd Ellah, Stefania Zanetti, Matthew Gavino Donadu
Summary: The wide spread of antibiotic resistance poses a serious global hazard, leading to millions of deaths. The emergence of multidrug resistant microorganisms is a result of the spread and sharing of resistance genes between different bacteria types. Biofilms created by microorganisms further enhance bacterial resistance and the development of multidrug resistant infections. The lack of novel antimicrobial compounds has led to international efforts in developing new antimicrobial agents and innovative techniques for antibiotic administration. Nanotechnology has shown potential in combating multidrug resistance and biofilm infections by utilizing nanomaterial-based therapeutics and their ability to target biofilms and resistant pathogens.
Article
Infectious Diseases
Abdelazeem M. Algammal, Nada H. Eidaroos, Khyreyah J. Alfifi, Marfat Alatawy, Alhanouf Al-Harbi, Yasmene F. Alanazi, Madeha O. Ghobashy, Ahmed R. Khafagy, Aboelkheir M. Esawy, Soha S. El-Sadda, Helal F. Hetta, Reham M. El-Tarabili
Summary: This study investigated the occurrence, oprL sequencing, antimicrobial resistance patterns, virulence-determinant, Quorum sensing, and antibiotic resistance genes of Pseudomonas aeruginosa from broiler chickens. The overall prevalence of P. aeruginosa in the examined birds was 28.3%. The recovered P. aeruginosa strains possessed various virulence-related genes, Quorum sensing genes, and resistance genes.
INFECTION AND DRUG RESISTANCE
(2023)
Review
Immunology
Abdulrahman K. K. Ahmed, Victor Coll Sijercic, Reem Sayad, Gregory R. R. Ruthig, Sayed F. F. Abdelwahab, Mohamed A. A. El-Mokhtar, Ibrahim M. M. Sayed
Summary: The study aimed to summarize the existing literature on the effects of COVID-19 on pregnant women, preterm babies, and the incidence of preterm birth (PTB). Contradictory results were found regarding the prevalence of PTB during the pandemic compared to previous years, with some studies showing an increase and others showing a decline. COVID-19 infection during pregnancy can lead to complications such as cesarean section, stillbirth, ICU admission, preeclampsia/eclampsia, and higher mortality rates. Vaccination for COVID-19 in pregnant and lactating women was found to stimulate immune responses without notable negative reactions or outcomes for the mother or baby.
Article
Microbiology
Amal A. Elkhawaga, Mohamed A. El-Mokhtar, Amal A. Mahmoud, Wael Esmat Ali, Doaa Safwat Mohamed, Ayat M. Kamel, Ahmed Atef Mesalam, Nermien H. S. Mousa, Ahmed M. Ashmawy, Essam M. Abdel Aziz, Ibrahim M. Sayed, Haidi Karam-Allah Ramadan, Yasmine Samy Elkholy
Summary: Impaired renal functions have been reported in patients with Hepatitis E virus (HEV) infections, particularly with genotypes 3 and 4. This study evaluated kidney function parameters in patients with HEV genotype 1 (HEV-1) during the acute phase of infection. Out of 31 HEV-1 patients, 16% had abnormal kidney function tests (KFTs), with some patients showing decreased glomerular filtration rates. These abnormalities resolved during convalescence. Monitoring kidney function and potential renal complications is important during HEV-1 infections.
Review
Microbiology
Alaa Elsaghir, Ehsan M. W. El-Sabaa, Abdulrahman K. Ahmed, Sayed F. Abdelwahab, Ibrahim M. Sayed, Mohamed A. El-Mokhtar
Summary: CD39 is a marker of immune cells that hydrolyzes ATP into adenosine, exerting an immunosuppressive effect. CD39 plays a regulatory role in various immunologic cells through purinergic signaling pathways. CD39+ T cells are implicated in viral infections and their expression is an indicator of lymphocyte exhaustion. CD39 levels in T lymphocytes can predict disease progression and immune response to antiviral drugs, while the percentage of CD39 and CD73 in B lymphocytes and monocytes affects the status of viral infections.
Article
Biochemistry & Molecular Biology
Mostafa F. Al-Hakkani, Nourhan Ahmed, Alaa A. Abbas, Mohammad H. A. Hassan, Hossameldin A. Aziz, Ali M. Elshamsy, Hazim O. Khalifa, Mohamed A. Abdelshakour, Mohammed S. Saddik, Mahmoud M. A. Elsayed, Marwa A. Sabet, Mohamed A. El-Mokhtar, Mosa Alsehli, M. S. Amin, Ahmed M. Abu-Dief, Hamada H. H. Mohammed
Summary: A novel derivative of ciprofloxacin (Cpx) was synthesized and showed enhanced antibacterial properties compared to Cpx itself. The derivative demonstrated increased activity against S. aureus and B. subtilis. Docking studies supported these findings by showing stronger binding interactions with the gyrase enzyme.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Mahmoud A. Hashem, Badriyah S. Alotaibi, Mahmoud M. A. Elsayed, Manal E. Alosaimi, Amal K. Hussein, Maram H. Abduljabbar, Kyung-Tae Lee, Hamdy Abdelkader, Mohamed A. El-Mokhtar, Ahmed H. E. Hassan, Amany A. Abdel-Rheem, Amany Belal, Mohammed S. Saddik
Summary: Wound healing is a significant healthcare problem, and developing novel topical delivery platforms can help accelerate the healing process and improve patients' quality of life.
Review
Microbiology
Helal F. Hetta, Yasmin N. Ramadan, Israa M. S. Al-Kadmy, Noura H. Abd Ellah, Lama Shbibe, Basem Battah
Summary: A multidrug-resistant pathogenic yeast called Candida auris can quickly spread among hospitalized and immunodeficient patients, causing nosocomial outbreaks and potentially pandemic outbreaks in 45 nations with high mortality rates. It has become resistant to decontamination techniques and can survive for weeks in hospitals. Nanoparticles have emerged as a promising treatment for diseases caused by this newly discovered pathogen, and are currently being researched as an effective solution against C. auris.
Article
Rheumatology
Yasser El Miedany, Samia Salah, Hala M. Lotfy, Mohammed Hassan Abu-Zaid, Sally S. Mohamed, Sheren Esam Maher, Maha El Gaafary, Hala Abdulhady, Yomna Farag, Mervat Eissa, Ahmed Radwan, Basma M. Medhat, Dalia M. E. El Mikkawy, Waleed A. Hassan, Doaa Mosad Mosa, Ghada El Deriny, Mohamed Mortada, Naglaa S. Osman, Nermeen Ahmed Fouad, Youmna Ahmed Amer, Samah Ismail Nasef, Hend Abushady, Salwa Galal, Eiman Abd El-Latif, Dina Maria, Ahmed H. Shabana, Samar AbdAlhamed Tabra
Summary: This study aimed to reach a consensus among experts on the management approach for Kawasaki disease. Through the Delphi method, 17 treatment recommendations were gathered, along with proposed algorithms for managing different KD conditions.
EGYPTIAN RHEUMATOLOGY AND REHABILITATION
(2023)
