Article
Immunology
Anders J. Svendsen, Marie Christine Wulff Westergaard, Anette Holck Draborg, Rene Holst, Kirsten O. Kyvik, Marianne A. Jakobsen, Peter Junker, Gunnar Houen
Summary: The study revealed distinct differences in EBNA1 antibody levels between patients with RA and healthy individuals, as well as between relatives predisposed to RA and healthy subjects. The levels of EBNA1 antibodies associated with RA and family predisposition to RA are influenced by both genetics (including the shared epitope) and environmental factors.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Seifegebriel Teshome, Elshafa Hassan Ahmed, Kidist Zealiyas, Abdulaziz Abubeker, Fisihatsion Tadesse, Christoph Weigel, Robert A. Baiocchi, Tamrat Abebe
Summary: This study investigated the genetic variation of EBV in lymphoma patients in Ethiopia. The results showed a higher distribution of EBV genotype 1 in these patients, and a significant association between age groups and EBV genotypes was observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Huanzhou Xu, Xiaofan Li, Beth A. Rousseau, Ibukun A. Akinyemi, Tiffany R. Frey, Kevin Zhou, Lauren E. Droske, Jennifer A. Mitchell, Michael T. McIntosh, Sumita Bhaduri-McIntosh
Summary: IFI16 is a nuclear DNA sensor that mediates antiviral responses by activating the inflammasome, triggering an interferon response, and silencing lytic genes of herpesviruses. It helps maintain latency of the Epstein-Barr virus (EBV) through H3K9me3 heterochromatinization via unknown mechanisms.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Kidist Zealiyas, Seifegebriel Teshome, Aklilu Feleke Haile, Christoph Weigel, Ayinalem Alemu, Wondwossen Amogne, Getnet Yimer, Tamrat Abebe, Nega Berhe, Elshafa Hassan Ahmed, Robert A. Baiocchi
Summary: In Ethiopia, EBV genotype 1 was found to be the most predominant genotype, followed by EBV genotype 2. Understanding the genotype characterization of EBV in PWH is essential for developing new and innovative strategies for preventing and treating EBV-related complications in this population.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Virology
Yang Mei, Troy E. Messick, Jayaraju Dheekollu, Hee Jong Kim, Sudheer Molugu, Leonardo Josue Castro Munoz, Vera Moiskeenkova-Bell, Kenji Murakami, Paul M. Lieberman
Summary: EBV latent infection is associated with various diseases. EBNA1 is a viral protein that plays a crucial role in maintaining the virus during latent infection. In this study, the structure of EBNA1 DNA-binding domain (DBD) and its interaction with different sites were determined using cryo-EM. The N-terminal domain (NTD) of EBNA1 was also found to interact with specific DNA sequences. These findings provide insights into the mechanisms of EBNA1 binding and may have implications for targeting EBNA1 in EBV-associated diseases.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Seifegebriel Teshome, Kidist Zealiyas, Abdulaziz Abubeker, Fisihatsion Tadesse, Jayalakshmi Balakrishna, Christoph Weigel, Tamrat Abebe, Elshafa Hassan Ahmed, Robert A. Baiocchi
Summary: This study investigated the presence and viral load of Epstein-Barr virus (EBV) in lymphoma patients from Ethiopia using molecular and serological approaches. EBV DNA was detected in 99% of patients, and serological analyses showed EBV presence in 96% of cases. A high EBV viral load was observed in more than half of the patients.
Review
Cell Biology
Tak-Yu Yau, William Sander, Christian Eidson, Albert J. Courey
Summary: SUMO modulates protein function by covalently conjugating to proteins and recruiting other cellular proteins. The recruitment involves SIM/SUMO interaction, where SIM consists of hydrophobic residues and binds to a hydrophobic groove in SUMO.
Review
Biochemistry & Molecular Biology
Nan Zhang, Yuxin Zuo, Liping Jiang, Yu Peng, Xu Huang, Lielian Zuo
Summary: This paper reviews the mechanisms underlying the role of EBV in the development of central nervous system diseases, discussing its effects on neurons and glial cells, as well as its promotion of proliferative disorders in B and T lymphocytes. However, the specific underlying molecular mechanisms remain unclear.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Prachi Deshmukh, Shubha Markande, Vikas Fandade, Yogendra Ramtirtha, Mallur Srivatsan Madhusudhan, Jomon Joseph
Summary: Micro-RNA mediated suppression of mRNA translation is a key regulatory mode of gene expression. Nup358-SIM has multiple interacting regions on AGO2, facilitating the coupling of miRISC with target mRNA. Specific residues are involved in the interaction of AGO2 with miRNA-loading components.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Virology
Weiyue Ding, Chong Wang, Yohei Narita, Hongbo Wang, Merrin Man Long Leong, Alvin Huang, Yifei Liao, Xuefeng Liu, Yusuke Okuno, Hiroshi Kimura, Benjamin Gewurz, Mingxian Teng, Shuilin Jin, Yoshitaka Sato, Bo Zhao
Summary: This study utilized HiChIP technology to investigate the 3D conformation of EBV in different cancer cell lines and identified associated genes with EBV enhancers. These findings provide new insights into the regulation of gene expression in EBV-associated tumors.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Viktoryia Laurynenka, Lili Ding, Kenneth M. Kaufman, Judith A. James, John B. Harley
Summary: The association between EBV infection and SLE is reconfirmed, and the presence of anti-EBNA1 antibodies in SLE cases is more frequent than in EBV-infected controls, supporting the hypothesis that anti-EBNA1 contributes to SLE.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Hani Mosayebzadeh Roshan, Reza Valadan, Seyed Abdollah Hosseini, Abolghasem Ajami
Summary: The seroprevalence of anti-EBV antibodies is higher in multiple sclerosis patients compared to normal individuals. More specifically, the titer of anti EBNA1 antibody, an indicator of past EBV infection, is also higher in multiple sclerosis patients. However, this antibody titer difference is not reliable for distinguishing between multiple sclerosis patients and non-multiple sclerosis individuals. Studies have identified specific epitopes on EBNA1 that are targeted by anti-EBNA1 antibodies in multiple sclerosis patients, and measuring antibody response against these epitopes can help better discriminate between the two groups.
JOURNAL OF NEUROIMMUNOLOGY
(2023)
Article
Microbiology
Danijela Miljanovic, Andja Cirkovic, Ivica Jermic, Milica Basaric, Ivana Lazarevic, Milka Grk, Rada Miskovic, Aleksa Despotovic, Ana Banko
Summary: The study aimed to analyze the association between EBV and RA by examining antibody titers, DNA viremia, infection status and EBNA1 variants in RA patients. The results showed a higher prevalence of active/recent EBV infection in RA patients compared to controls, along with elevated levels of specific antibodies. The findings suggest the need for further research to understand the causal relationship between EBV and RA. Evaluation: 8/10.
Review
Immunology
Alison J. Sinclair
Summary: DNA methylation at CpG motifs plays a key role in gene regulation, with hypermethylation generally leading to decreased gene expression. In EBV infection, the viral genome becomes hypermethylated at CpG motifs, impacting viral gene expression. DNA methyl transferase enzymes are crucial in the process of DNA methylation and can be targeted for therapeutic interventions in EBV-associated cancer.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Virology
Kathy Shire, Edyta Marcon, Jack Greenblatt, Lori Frappier
Summary: Analysis of Epstein-Barr virus genomes revealed three amino acid changes in EBNA1 associated with gastric carcinoma and nasopharyngeal carcinoma. One of these mutations, Thr85Ala, was found to decrease transcriptional activation and have a gain of function interaction with PLOD1 and PLOD3, proteins linked to multiple cancers. Identifying the interaction between EBNA1 and PLOD1 provides new insights into the connection between EBV infection and cancer.