ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons

In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation

(2019) Erin F. Spence et al.   Nature Communications

Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats

(2019) Susanne Bäck et al.   NEURON

AMPA receptors and their minions: auxiliary proteins in AMPA receptor trafficking

(2019) Diane Bissen et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

VAP‐SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function

(2019) Feline W Lindhout et al.   EMBO JOURNAL

Imaging endogenous synaptic proteins in primary neurons at single-cell resolution using CRISPR/Cas9

(2019) Takahiko Matsuda et al.   MOLECULAR BIOLOGY OF THE CELL

An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity

(2019) Jochen Schwenk et al.   NEURON

RIM C 2 B Domains Target Presynaptic Active Zone Functions to PIP 2 -Containing Membranes

(2018) Arthur P.H. de Jong et al.   NEURON

An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein

(2018) Pooran Singh Dewari et al.   eLife

Differential Nanoscale Topography and Functional Role of GluN2-NMDA Receptor Subtypes at Glutamatergic Synapses

(2018) Blanka Kellermayer et al.   NEURON

Visualizing and discovering cellular structures with super-resolution microscopy

(2018) Yaron M. Sigal et al.   SCIENCE

CRISPR-Cas guides the future of genetic engineering

(2018) Gavin J. Knott et al.   SCIENCE

The HAUS Complex Is a Key Regulator of Non-centrosomal Microtubule Organization during Neuronal Development

(2018) Inês Cunha-Ferreira et al.   Cell Reports

Predictable and precise template-free CRISPR editing of pathogenic variants

(2018) Max W. Shen et al.   NATURE

Fluorescence nanoscopy in cell biology

(2017) Steffen J. Sahl et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Transcellular Nanoalignment of Synaptic Function

(2017) Thomas Biederer et al.   NEURON

Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain

(2017) Jun Nishiyama et al.   NEURON

Optogenetic Control of Synaptic Composition and Function

(2017) Brooke L. Sinnen et al.   NEURON

Structural organization of the actin-spectrin–based membrane skeleton in dendrites and soma of neurons

(2017) Boran Han et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

AMPA-receptor specific biogenesis complexes control synaptic transmission and intellectual ability

(2017) Aline Brechet et al.   Nature Communications

Dynamic disorganization of synaptic NMDA receptors triggered by autoantibodies from psychotic patients

(2017) Julie Jézéquel et al.   Nature Communications

Ferric Chelate Reductase 1 Like Protein (FRRS1L) Associates with Dynein Vesicles and Regulates Glutamatergic Synaptic Transmission

(2017) Wenyan Han et al.   Frontiers in Molecular Neuroscience

High-Throughput, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing

(2016) Takayasu Mikuni et al.   CELL

DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks

(2016) Megan van Overbeek et al.   MOLECULAR CELL

In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration

(2016) Keiichiro Suzuki et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

The rules and impact of nonsense-mediated mRNA decay in human cancers

(2016) Rik G H Lindeboom et al.   NATURE GENETICS

GSG1L suppresses AMPA receptor-mediated synaptic transmission and uniquely modulates AMPA receptor kinetics in hippocampal neurons

(2016) Xinglong Gu et al.   Nature Communications

Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques

(2016) Takeshi Uemura et al.   Scientific Reports

Optogenetic control of organelle transport and positioning

(2015) Petra van Bergeijk et al.   NATURE

A mechanism for the suppression of homologous recombination in G1 cells

(2015) Alexandre Orthwein et al.   NATURE

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

STED Nanoscopy Reveals the Ubiquity of Subcortical Cytoskeleton Periodicity in Living Neurons

(2015) Elisa D’Este et al.   Cell Reports

CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling

(2014) Randall J. Platt et al.   CELL

Multiple Spatial and Kinetic Subpopulations of CaMKII in Spines and Dendrites as Resolved by Single-Molecule Tracking PALM

(2014) H. E. Lu et al.   JOURNAL OF NEUROSCIENCE

Directed evolution of APEX2 for electron microscopy and proximity labeling

(2014) Stephanie S Lam et al.   NATURE METHODS

Microhomology-based choice of Cas9 nuclease target sites

(2014) Sangsu Bae et al.   NATURE METHODS

Microtubule Minus-End Binding Protein CAMSAP2 Controls Axon Specification and Dendrite Development

(2014) Kah Wai Yau et al.   NEURON

Is Non-Homologous End-Joining Really an Inherently Error-Prone Process?

(2014) Mireille Bétermier et al.   PLoS Genetics

Local palmitoylation cycles define activity-regulated postsynaptic subdomains

(2013) Yuko Fukata et al.   JOURNAL OF CELL BIOLOGY

Recombinant Probes Reveal Dynamic Localization of CaMKII  within Somata of Cortical Neurons

(2013) R. J. Mora et al.   JOURNAL OF NEUROSCIENCE

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation

(2013) John Y Lin et al.   NATURE NEUROSCIENCE

Nanoscale Scaffolding Domains within the Postsynaptic Density Concentrate Synaptic AMPA Receptors

(2013) Harold D. MacGillavry et al.   NEURON

Recombinant Probes for Visualizing Endogenous Synaptic Proteins in Living Neurons

(2013) Garrett G. Gross et al.   NEURON

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells

(2012) Kyle J. Roux et al.   JOURNAL OF CELL BIOLOGY

Immunolabeling artifacts and the need for live-cell imaging

(2012) Ulrike Schnell et al.   NATURE METHODS

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Mechanisms of CaMKII action in long-term potentiation

(2012) John Lisman et al.   NATURE REVIEWS NEUROSCIENCE

High-Resolution Proteomics Unravel Architecture and Molecular Diversity of Native AMPA Receptor Complexes

(2012) Jochen Schwenk et al.   NEURON

Actin, Spectrin, and Associated Proteins Form a Periodic Cytoskeletal Structure in Axons

(2012) K. Xu et al.   SCIENCE

Differences in AMPA and Kainate Receptor Interactomes Facilitate Identification of AMPA Receptor Auxiliary Subunit GSG1L

(2012) Natalie F. Shanks et al.   Cell Reports

A practical guide to evaluating colocalization in biological microscopy

(2011) Kenneth W. Dunn et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

Dynamic Superresolution Imaging of Endogenous Proteins on Living Cells at Ultra-High Density

(2010) Gregory Giannone et al.   BIOPHYSICAL JOURNAL

SAP102 Is a Highly Mobile MAGUK in Spines

(2010) C. Y. Zheng et al.   JOURNAL OF NEUROSCIENCE

Sequence signatures and mRNA concentration can explain two-thirds of protein abundance variation in a human cell line

(2010) Christine Vogel et al.   Molecular Systems Biology

Single-Molecule Discrimination of Discrete Perisynaptic and Distributed Sites of Actin Filament Assembly within Dendritic Spines

(2010) Nicholas A. Frost et al.   NEURON

Defining mechanisms of actin polymerization and depolymerization during dendritic spine morphogenesis

(2009) Pirta Hotulainen et al.   JOURNAL OF CELL BIOLOGY

Molecular Architecture of Synaptic Actin Cytoskeleton in Hippocampal Neurons Reveals a Mechanism of Dendritic Spine Morphogenesis

(2009) Farida Korobova et al.   MOLECULAR BIOLOGY OF THE CELL