Review
Biophysics
Vanessa E. Abrantes-Coutinho, Andre O. Santos, Rafael B. Moura, Francisco N. Pereira-Junior, Lucia H. Mascaro, Simone Morais, Thiago M. B. F. Oliveira
Summary: Lectin-based biosensors have made significant progress in the field of electroanalysis, offering high sensitivity, specificity, precision, and stability for the detection of clinically relevant carbohydrates and glycoproteins. By combining different materials and functional groups, these sensors can further enhance detection performance and applicability.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Pharmacology & Pharmacy
Charlotte Sorieul, Francesco Papi, Filippo Carboni, Simone Pecetta, Sanjay Phogat, Roberto Adamo
Summary: Carbohydrates are widely expressed on the surface of cells and play important roles in immune responses and cancer progression. Vaccines and monoclonal antibodies targeting specific carbohydrates have been developed, but the immune tolerance associated with these glycans poses challenges. New strategies are being explored to enhance carbohydrate-based cancer therapies.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Chemistry, Multidisciplinary
Shuo Zhang, Mauro Sella, Julinton Sianturi, Patricia Priegue, Dacheng Shen, Peter H. Seeberger
Summary: Researchers synthesized oligosaccharides resembling the capsular polysaccharides of Streptococcus suis serotypes and identified lead antigens for the development of semi-synthetic S. suis serotypes 2 and 9 glycoconjugate veterinary vaccines by screening blood from infected pigs.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Microbiology
Luther A. Bartelt, Alena J. Markmann, Bridget Nelson, Jessica Keys, Heather Root, Heather Henderson, JoAnn Kuruc, Caroline Baker, D. Ryan Bhowmik, Yixuan J. Hou, Lakshmanane Premkumar, Caleb Cornaby, John L. Schmitz, Susan Weiss, Yara Park, Ralph Baric, Aravinda M. de Silva, Anne Lachiewicz, Sonia Napravnik, David van Duin, David M. Margolis
Summary: In this study, it was found that among high-risk patients admitted for COVID-19, those who received CCP with nAb titers of >1:640 had earlier hospital discharge compared to those who received CCP with lower nAb titers, with no CCP-related adverse events. The significance of this research lies in identifying a dose response of CCP and clinical outcomes based on nAb titers. Although limited by a small study size, these findings support further investigation of high-nAb-titer CCP (>1:640) in the treatment of COVID-19.
Article
Chemistry, Organic
David Lim, Francesca Paradisi
Summary: Glycosylation can improve the pharmacokinetic properties of bioactive molecules, but their syntheses often require tedious protecting group manipulations. In this study, we developed a method for direct aqueous conversion of unprotected sugars into glycosides, and demonstrated its application with various biomolecules including resorcinol, resveratrol, and gimeracil. We also showed that the bioactive drug can be released from the sugar in an oxidatively-triggered manner, making them suitable for medicinal applications. This novel transformation provides access to various selenium-containing glycomimetics and glycoconjugates.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2023)
Letter
Medicine, General & Internal
Masaki Imai, Mutsumi Ito, Maki Kiso, Seiya Yamayoshi, Ryuta Uraki, Shuetsu Fukushi, Shinji Watanabe, Tadaki Suzuki, Ken Maeda, Yuko Sakai-Tagawa, Kiyoko Iwatsuki-Horimoto, Peter J. Halfmann, Yoshihiro Kawaoka
Summary: The antiviral agents bebtelovimab and mAb combinations tested in the study did not neutralize the SARS-CoV-2 omicron subvariants BQ.1.1 and XBB, while remdesivir, molnupiravir, and nirmatrelvir demonstrated efficacy against both subvariants in vitro.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Immunology
Armelle Phalipon, Laurence A. Mulard
Summary: This review discusses the molecular glycovaccine concept and its potential to develop a Shigella glycoconjugate vaccine. Using chemically synthesized oligosaccharides attached to a carrier, a fine-tuned Shigella glycovaccine prototype was developed and tested in clinical trials, showing good safety and bactericidal effects. Challenges in advancing this novel approach to a multivalent, cost-effective Shigella synthetic glycan conjugate vaccine are also discussed.
Review
Food Science & Technology
Siyu Chen, Dan Li
Summary: This study reviewed the potential of complex carbohydrates in combating noroviruses and identified 15 publications that explored this topic. The findings suggested that certain carbohydrates, such as Inonotus obliquus polysaccharide, Houttuynia cordata polysaccharide, chitosan, human milk oligosaccharides, fucoidan, and neoagarohexaose NA6, have anti-norovirus effects either through direct interactions with the viruses or by enhancing the host's innate immune response. The study also highlighted the knowledge gaps in terms of evaluation systems and the structural diversity of complex carbohydrates.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Review
Biochemistry & Molecular Biology
Eugeniy P. Smorodin
Summary: Synthetic glycoconjugates are accurate tools for studying glycan-binding proteins, but there are challenges in the specificity of tumor-associated glycans and anti-glycan antibodies. Bacteria contribute to the stimulus of AG Abs and cancer progression, posing challenges in interactions with tumors and dysregulated immune responses. The integrative study of AG Abs and the microbiome using high-performance technologies presents possibilities and problems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Christine M. Szymanski
Summary: Each microbe can produce a wide variety of sugar structures that are distinct from those in eukaryotes. Differences in carbohydrate building blocks and additional mechanisms for synthesis contribute to the structural heterogeneity of individual strains. Past research focused more on human pathogens, but recent advances in technology have highlighted the importance of studying microbial glycan diversity in commensals and environmental isolates.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Microbiology
Paulina Koszalka, Ankita George, Vijaykrishna Dhanasekaran, Aeron C. Hurt, Kanta Subbarao
Summary: Combination therapy with influenza drugs baloxavir and oseltamivir can reduce the selection of viruses with reduced drug susceptibility. In animal models, combination therapy and monotherapy have similar effectiveness in reducing viral titers, but combination therapy can decrease the selection of viruses with reduced susceptibility to baloxavir.
Article
Microbiology
Nicole N. Haese, Nicholas A. May, Sharon Taft-Benz, Omar Moukha-Chafiq, Nikhil Madadi, Sixue Zhang, Shuklendu D. Karyakarte, Kevin J. Rodzinak, Theresa H. Nguyen, Michael Denton, Aaron D. Streblow, Nichole A. Towers, Lynn Rasmussen, Robert J. Bostwick, Joseph A. Maddry, Subramaniam Ananthan, Corinne E. Augelli-Szafran, Mark J. Suto, Wes Sanders, Nathaniel Moorman, Victor DeFilippis, Mark T. Heise, Ashish K. Pathak, Daniel N. Streblow, Thomas E. Morrison
Summary: A quinolinone compound was found to have antiviral activity against VEEV and other alphaviruses, inhibiting the early-to-mid stage of viral replication. Deep sequencing and reverse genetics studies identified unique resistance mutations in the nsP2 gene that conferred VEEV resistance on this chemical series, while computational modeling indicated potential docking alignment for the compounds.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Chemistry, Organic
Debasish Pal, Aritra Chaudhury, Balaram Mukhopadhyay
Summary: This manuscript reports the chemical synthesis method of the trisaccharide repeating unit of the O-antigen from Pseudomonas putida BIM B-1100. The challenging residues were successfully incorporated through rational protecting group manipulations and a linear strategy, and the desired carboxylic acid moiety was introduced through TEMPO-mediated oxidation. The use of aminoethyl glycoside allows further glycoconjugate formation without affecting the anomeric stereochemistry.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Organic
Anjali Aghi, Akanksha Singh Baghel, Amit Kumar
Summary: We report a metal-free, diastereoselective method for synthesizing conformationally locked spiro bicyclic sugars with a quaternary center at the C5 position. The method involves the one-step construction of an oxazoline-containing spiro-bicyclic system from easily synthesizable C6-glycosylimidates under visible light conditions. Key features of this protocol include mild reaction conditions, green energy source, broad substrate scope, scalable synthesis, and high reaction yields.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Microbiology
Sean M. Amberg, Benjamin Snyder, Portia A. Vliet-Gregg, Eric J. Tarcha, Jeff Posakony, Kristin M. Bedard, Alison E. Heald
Summary: LHF-535, a small-molecule antiviral, is being developed as a potential treatment for Lassa fever and other viral hemorrhagic fevers. Phase 1 trials in healthy volunteers demonstrated that LHF-535 was well tolerated with rapid absorption and a long half-life. This drug has the potential to suppress viral replication.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Chemistry, Organic
Chang-Xin Huo, Xiu-Jing Zheng, An Xiao, Chang-Cheng Liu, Shuang Sun, Zhuo Lv, Xin-Shan Ye
ORGANIC & BIOMOLECULAR CHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Chang-Cheng Liu, Canjia Zhai, Xiu-Jing Zheng, Xin-Shan Ye
ACS CHEMICAL BIOLOGY
(2016)
Article
Oncology
Chengcheng Song, Xiu-Jing Zheng, Chang-Cheng Liu, Yifa Zhou, Xin-Shan Ye
Article
Chemistry, Multidisciplinary
Caicai Meng, Aniruddha Sasmal, Yan Zhang, Tian Gao, Chang-Cheng Liu, Naazneen Khan, Ajit Varki, Fengshan Wang, Hongzhi Cao
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2018)
Article
Chemistry, Multidisciplinary
Na Lu, Jinfeng Ye, Jiansong Cheng, Aniruddha Sasmal, Chang-Cheng Liu, Wenlong Yao, Jun Yan, Naazneen Khan, Wen Yi, Ajit Varki, Hongzhi Cao
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Physical
Jinfeng Ye, Hui Xia, Na Sun, Chang-Cheng Liu, Anran Sheng, Lianli Chi, Xian-Wei Liu, Guofeng Gu, Shu-Qi Wang, Jie Zhao, Ping Wang, Min Xiao, Fengshan Wang, Hongzhi Cao
Article
Chemistry, Multidisciplinary
Tian Gao, Jingyu Yan, Chang-Cheng Liu, Angelina S. Palma, Zhimou Guo, Min Xiao, Xi Chen, Xinmiao Liang, Wengang Chai, Hongzhi Cao
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Biochemistry & Molecular Biology
Weiying Hong, Chang-Cheng Liu, Henan Zhang, Zhiyong Chen, Min Xiao, Li Xu
Summary: A novel delivery system derived from oligorutin (OR) was developed for cancer cell targeting and pH-responsive drug delivery. OR showed tumor-targeting property and pH-responsive drug delivery capability. OR-BTZ, a pH-responsive drug delivery system, exhibited cytotoxicity and inhibition of migration and invasion in hepatoma carcinoma cells.
Article
Chemistry, Multidisciplinary
Wenyuan Fang, Kan Zhong, Jiansong Cheng, Xian-Wei Liu, Chang-Cheng Liu, Zhongfu Wang, Hongzhi Cao
Summary: A convenient and efficient strategy was developed for the simplification of enzymatic synthesis and purification of oligosaccharides. The method involves extending glycosyl acceptors terminated with a thiol tag through enzymatic modular assembly in the aqueous phase, capturing the desired products during solid-phase workup using commercially available thiopropyl Sepharose 6B resin, and releasing the products in the presence of dithiothreitol (DTT) as a reducing agent. This strategy not only overcomes the uncertain influence of commonly used polymer- or ionic-supports in enzymatic glycosylation, but also shows perfect compatibility with all 10 enzyme modules for rapid assembly of complex oligosaccharides.
CHINESE JOURNAL OF CHEMISTRY
(2022)
Article
Biochemical Research Methods
Chang-Cheng Liu, Chang-Xin Huo, Canjia Zhai, Xiu-Jing Zheng, De-Cai Xiong, Xin-Shan Ye
Summary: The study focuses on the glycosylation and conformational limitations of the HIV-1 envelope protein. A synthetic approach for Man5 and its analogues was developed, and their immunogenicity was evaluated in mice. The modified analogues induced stronger immune responses but failed to bind the native antigen.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Na Lu, Yun Li, Hui Xia, Kan Zhong, Chenning Jia, Jinfeng Ye, Xianwei Liu, Chang-Cheng Liu, Hongzhi Cao
Summary: This study presents a facile and general substrate engineering strategy for site-specific alpha 1,3-fucosylation of complex glycans containing multiple LacNAc units. The strategy utilizes galactose oxidase (GOase) to convert the LacNAc unit into an oxidized C6'-aldehyde LacNAc sequence, enabling site-specific alpha 1,3-fucosylation at intact LacNAc sites. The strategy demonstrates general applicability and robustness for synthesizing well-defined fucosides of linear and branched O- and N-linked glycans.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Organic
Hui Xia, Jinfeng Ye, Hongzhi Cao, Xianwei Liu, Yan Zhang, Chang-Cheng Liu
Summary: The enzymatic synthesis of hybrid Lewis antigens was achieved using a modular assembly strategy, providing easy access to complex hybrid Lewis antigens at preparative scales. The regio-selective fucosylation was achieved by controlling the donor-acceptor ratio.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Kan Zhong, Jinfeng Ye, Xinhao Zhu, Hongzhi Cao, Chang-Cheng Liu
Article
Chemistry, Multidisciplinary
Congcong Chen, Shuaishuai Wang, Madhusudhan Reddy Gadi, He Zhu, Fei Liu, Chang-Cheng Liu, Lei Li, Fengshan Wang, Peixue Ling, Hongzhi Cao
CHEMICAL COMMUNICATIONS
(2020)