Article
Microbiology
Shiwen Peng, Deyin Xing, Louise Ferrall, Ya-Chea Tsai, Richard B. S. Roden, Chien-Fu Hung, T. -C. Wu
Summary: Our research focused on eliminating the confounding effects of dominant HPV antigen presentation through murine MHC-I via epitope mutagenesis, while ensuring oncogenicity. Mutated DNA constructs of HPV16 E6 and E7 were able to abolish murine MHC-I presentation and instead use human HLA-A2 molecules for presentation. These mutated constructs still maintained their oncogenic properties.
Article
Microbiology
Shiwen Peng, Deyin Xing, Louise Ferrall, Ya-Chea Tsai, Richard B. S. Roden, Chien-Fu Hung, T-C Wu
Summary: The study developed a spontaneous HPV16-driven HNSCC model in HLA-A2 transgenic mice to test candidate immunotherapies. They eliminated the confounding effects of dominant HPV antigen presentation through epitope mutagenesis and characterized their presentation in murine and human MHC-I molecules.
Article
Oncology
Eleni M. Rettig, Daniel L. Faden, Shaiba Sandhu, Kristine Wong, William C. Faquin, Chloe Warinner, Phil Stephens, Sunil Kumar, Charlotte Kuperwasser, Jeremy D. Richmon, Ravindra Uppaluri, Mark Varvares, Rosh Sethi, Glenn J. Hanna, Herve Sroussi
Summary: This study found that circulating tumor HPV DNA can be detected at least several years before the diagnosis of HPV-positive head and neck squamous cell carcinoma (HNSCC) in some patients, which may serve as a biomarker for early diagnosis of HPV16-positive HNSCC.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Oncology
Jitesh B. Shewale, Robert K. L. Pickard, Weihong Xiao, Bo Jiang, Maura L. Gillison
Summary: This case-control study confirmed and extended the distinct risk-factor profiles for HPV-positive and HPV-negative OSCC, supporting two principal etiological pathways for OSCC development. Sexual behavior and education were associated with HPV-positive cancer, while low annual income, infrequent dental visits, and tobacco and alcohol use were associated with HPV-negative cancers. Long-term marijuana use appeared protective for HPV-negative cancer. Public health efforts targeting these modifiable risk factors may help prevent oral cancer.
Article
Oncology
Imogen Sharkey Ochoa, Esther O'Regan, Mary Toner, Elaine Kay, Peter Faul, Connor O'Keane, Roisin O'Connor, Dorinda Mullen, Mataz Nur, Eamon O'Murchu, Jacqui Barry-O'Crowley, Niamh Kernan, Prerna Tewari, Helen Keegan, Sharon O'Toole, Robbie Woods, Susan Kennedy, Kenneth Feeley, Linda Sharp, Tarik Gheit, Massimo Tommasino, John J. O'Leary, Cara M. Martin
Summary: HPV infection has been identified as a significant factor in the development of head and neck squamous cell carcinoma (HNSCC). HPV's involvement seems to be associated with better survival and prognosis in patients and suggests the need for different treatment strategies. Limited data on HPV infection in Europe exists, so this study aimed to investigate HPV's impact on HNSCC patient outcomes in the Irish population, providing important findings for further research and treatment pathways for HPV-related HNSCC.
Article
Otorhinolaryngology
Koji Ebisumoto, Akihiro Sakai, Daisuke Maki, Kevin Robinson, Tomoaki Murakami, Hiroaki Iijima, Mayu Yamauchi, Kosuke Saito, Takane Watanabe, Kenji Okami
Summary: The study retrospectively included 85 CUP patients from Tokai University and found that the overall primary detection rate did not significantly improve with the addition of transoral examination. However, a greater number of oropharyngeal primaries were detected, which were mainly p16 positive and located on the palatine tonsil. Therefore, transoral examination is a promising technique to improve tumor detection for human papillomavirus-associated head and neck cancers.
LARYNGOSCOPE INVESTIGATIVE OTOLARYNGOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Jason Tasoulas, Sonal Srivastava, Xiaonan Xu, Valentina Tarasova, Anastasios Maniakas, Florian A. Karreth, Antonio L. Amelio
Summary: The head and neck region is highly susceptible to cancer, with over 1.5 million new cases reported worldwide in 2020. Significant progress has been made in understanding the disease mechanisms and tailoring treatments to individual tumor characteristics using genetically engineered mouse models. These models have successfully replicated various aspects of head and neck cancers.
Article
Oncology
Loretto J. Carvajal, Jaimie Z. Shing, Juan C. Vanegas, Emmanuel Gonzalez, Diego Guillen, Monica S. Sierra, Allan Hildesheim, Carolina Porras, Rolando Herrero, Guillermo Torres, Meredith S. Shiels, Alejandro Calderon, Aimee R. Kreimer
Summary: This study investigated the incidence and trends of head and neck cancer in Costa Rica, specifically focusing on the potentially human papillomavirus (HPV)-related and HPV-unrelated head and neck squamous cell carcinomas (HNSCC). The findings showed a decreasing incidence of HNSCC overall, but an increasing trend for HPV-related HNSCCs. This study is important for understanding the epidemiology of head and neck cancer and the impact of HPV.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Review
Oncology
Sandhya Kalavacherla, Parag Sanghvi, Grace Y. Y. Lin, Theresa Guo
Summary: Squamous cell carcinoma (SCC) from an unknown primary tumor (SCCUP) accounts for 2.0%-5.0% of all head and neck cancers. SCCUP presents as enlarged cervical lymph nodes without evidence of a primary tumor upon physical examination. Primary site detection is important to target treatment and avoid treatment-related morbidity.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
S. S. Mashiana, P. Navale, B. Khandakar, S. Sobotka, M. R. Posner, B. A. Miles, W. Zhang, M. Gitman, R. L. Bakst, E. M. Genden, W. H. Westra
Summary: This study examined the impact of HPV genotypes on patients with head and neck squamous cell carcinoma (HNSC) and found that different genotypes are unevenly distributed across anatomical sites, with specific associations with tumor characteristics. The presence of HPV18 was associated with transformation to an aggressive small cell phenotype, highlighting the variability in HPV genotypes and their potential impact on clinical behaviors. This challenges the assumption of HPV equivalency across all high-risk genotypes and may inform preventive, diagnostic, therapeutic, and surveillance strategies.
Review
Oncology
Tingting Qin, Shiting Li, Leanne E. Henry, Siyu Liu, Maureen A. Sartor
Summary: This study highlights the increasing incidence of HPV infections in head and neck cancers, with a focus on the molecular subtypes of HPV-positive HNC and their implications on treatment and prognosis.
Article
Virology
Renee E. King, Ella T. Ward-Shaw, Rong Hu, Paul F. Lambert, Susan L. Thibeault
Summary: A murine model of laryngeal MmuPV1 infection provides a useful preclinical model for studying recurrent respiratory papillomatosis (RRP) and developing potential treatments for this devastating disease.
Article
Immunology
Jana Mourtada, Christelle Lony, Anais Nicol, Justine De Azevedo, Cyril Bour, Christine Macabre, Patrick Roncarati, Sonia Ledrappier, Philippe Schultz, Christian Borel, Mickael Burgy, Bohdan Wasylyk, Georg Mellitzer, Michael Herfs, Christian Gaiddon, Alain C. Jung
Summary: This study identifies the molecular mechanisms underlying the heterogeneous prognosis of HPV-positive OSCC, providing a theoretical basis for the development of biomarkers and targeted therapies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Dayson Moreira, Sagus Sampath, Haejung Won, Seok Voon White, Yu-Lin Su, Marice Alcantara, Chongkai Wang, Peter Lee, Ellie Maghami, Erminia Massarelli, Marcin Kortylewski
Summary: The study suggests that targeted inhibition of STAT3 in tumor-associated myeloid cells enhances the efficacy of radiotherapy against HNSCC by promoting the recruitment of CD8(+) T cells and suppressing tumor growth.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Oncology
Mari Mylly, Linda Nissi, Teemu Huusko, Johannes Routila, Samuli Vaittinen, Heikki Irjala, Ilmo Leivo, Sami Ventela
Summary: This study investigated the prevalence and associations of p16 positivity with survival in HNSCC patients in Southwest Finland between 2005 and 2015. The results showed that p16 positivity was mainly found in the oropharynx and was associated with an increased likelihood of lymph node metastasis, a smaller primary tumor size, and a sparse history of smoking and alcohol consumption. Regardless of p16 expression, all HNSCC patients benefited from combining chemotherapy and radiotherapy.
Review
Materials Science, Biomaterials
LaTonya R. Simon, Kristyn S. Masters
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2020)
Review
Multidisciplinary Sciences
Hannah M. Micek, Mike R. Visetsouk, Kristyn S. Masters, Pamela K. Kreeger
Article
Oncology
Mara H. O'Brien, Henry C. Pitot, Sang-Hyuk Chung, Paul F. Lambert, Norman R. Drinkwater, Andrea Bilger
Summary: Estrogen plays a protective role against liver cancer in females primarily through Estrogen Receptor-alpha rather than Estrogen Receptor-beta. Deficiency of Estrogen Receptor-alpha significantly increases the risk of hepatocellular carcinoma in females, while deletion specifically in hepatocytes maintains female-specific liver gene expression. The control of sex-specific liver gene expression by Esr1, which mediates liver cancer risk, involves cells other than hepatocytes.
Article
Audiology & Speech-Language Pathology
Renee E. King, Seth H. Dailey, Susan L. Thibeault
Summary: The study found that patients undergoing vocal fold procedures significantly decreased talking during postprocedure voice rest, but did not perfectly adhere to the recommendations. Communicative voice use decreases more than noncommunicative voice use during voice rest. Participants estimated a 98% reduction in voice use during voice rest at follow-up.
AMERICAN JOURNAL OF SPEECH-LANGUAGE PATHOLOGY
(2021)
Article
Audiology & Speech-Language Pathology
Renee E. King, Carolyn K. Novaleski, Bernard Rousseau
Summary: The study aimed to analyze the effect of recommended absolute voice rest duration on outcomes of microflap surgery for benign vocal fold lesions, as well as identify patient factors associated with postoperative voice outcomes. The results showed that the duration of recommended voice rest did not have any impact on VHI outcomes of the surgery.
AMERICAN JOURNAL OF SPEECH-LANGUAGE PATHOLOGY
(2022)
Article
Virology
Renee E. King, Ella T. Ward-Shaw, Rong Hu, Paul F. Lambert, Susan L. Thibeault
Summary: A murine model of laryngeal MmuPV1 infection provides a useful preclinical model for studying recurrent respiratory papillomatosis (RRP) and developing potential treatments for this devastating disease.
Article
Virology
Renee E. King, Andrea Bilger, Josef Rademacher, Ella T. Ward-Shaw, Rong Hu, Paul F. Lambert, Susan L. Thibeault
Summary: Recurrent respiratory papillomatosis (RRP), caused by laryngeal infection with low-risk human papillomaviruses, has devastating effects on vocal communication and quality of life. This study found that Mus musculus papillomavirus (MmuPV1) can infect the larynx and cause laryngeal disease, and epithelial injury enhances its ability to cause disease.
Article
Virology
Laura C. Gunder, Simon Blaine-Sauer, Hillary R. Johnson, Myeong-Kyun Shin, Andrew S. Auyeung, Wei Zhang, Glen E. Leverson, Ella T. Ward-Shaw, Renee E. King, Stephanie M. McGregor, Kristina A. Matkowskyj, Paul F. Lambert, Evie H. Carchman
Summary: Topical dihydroartemisinin (DHA) did not show efficacy in reducing genital dysplasia or preventing anal cancer in papillomavirus-induced mouse models.
Article
Cardiac & Cardiovascular Systems
LaTonya R. Simon, Ashley J. Scott, Lysmarie Figueroa Rios, Joshua Zembles, Kristyn S. Masters
Summary: Males are more prone to CAVD, but females exhibit higher levels of fibrosis. Male VICs produce higher levels of myofibroblastic markers, while female VICs show greater metabolic activity and collagen production. Females generally exhibit higher levels of MMP expression and production, but no sex differences are observed in TIMP production. Male VICs also display higher levels of collagenase and gelatinase activity compared to female VICs.
Review
Medicine, Research & Experimental
Renee E. King, Andrea Bilger, Josef Rademacher, Paul F. Lambert, Susan L. Thibeault
Summary: This study evaluated preclinical models of laryngeal papillomavirus infection, primarily involving low-risk HPV. Most models lose viral DNA after a short duration. Future research is needed to develop models for persistent and recurrent diseases, consistent with RRP and HPV-positive laryngeal cancer.
Article
Medicine, Research & Experimental
Renee E. King, Josef Rademacher, Ella T. Ward-Shaw, Rong Hu, Andrea Bilger, Simon Blaine-Sauer, Megan E. Spurgeon, Susan L. Thibeault, Paul F. Lambert
Summary: This study investigated secondary and vertical laryngeal infections of mouse papillomavirus in immunocompetent mice. The results showed no evidence of lateral spread of MmuPV1 to the larynx in mice infected in the oral cavity, oropharynx, or anus. Moreover, no laryngeal MmuPV1 infection was found in offspring born to infected mothers.
Article
Biochemical Research Methods
Pamela K. Kreeger, Amy Brock, Holly C. Gibbs, K. Jane Grande-Allen, Alice H. Huang, Kristyn S. Masters, Padmini Rangamani, Michaela R. Reagan, Shannon L. Servoss
PLOS COMPUTATIONAL BIOLOGY
(2020)
Review
Biotechnology & Applied Microbiology
Vaidehi A. Patil, Kristyn S. Masters
BIOENGINEERING-BASEL
(2020)