期刊
WORLD JOURNAL OF SURGICAL ONCOLOGY
卷 18, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12957-020-01884-x
关键词
Hepatocellular carcinoma cells; Apoptosis; Starvation; HIF1A-AS1; Autophagy
资金
- Zhejiang Provincial Natural Science Foundation of China [LY16H160004]
- Natural Science Foundation of Shanghai [17ZR1422500, 19ZR1456300]
- Shanghai Municipal Population and Family Planning Commission [201540304]
- Interdisciplinary Program of Shanghai Jiao Tong University [YG2016MS26]
BackgroundHepatocellular carcinoma (HCC) is still a major health burden in China considering its high incidence and mortality. Long non-coding RNAs (lncRNAs) were found playing vital roles in tumor progression, suggesting a new way of diagnosis and prognosis prediction, or treatment of HCC. This study was designed to investigate the role of HIF1A-AS1 during the progression of HCC and to explore its related mechanisms.MethodsThe expression of HIF1A-AS1 was detected in 50 paired carcinoma tissues and adjacent normal tissues by quantitative real-time PCR assay. HCC cell apoptosis was induced by nutrient-deficient culture medium and detected by Cell Counting Kit-8 and flow cytometer assays. HIF1A-AS1 inhibition in HCC cells was accomplished by small interfering RNA transfection.ResultsHIF1A-AS1 was overexpressed in HCC tissues and was associated with tumor size, TNM stage, and lymph node metastasis. Compared with the low HIF1A-AS1 group, the high HIF1A-AS1 group had a shorter overall survival and a worse disease-free survival. HIF1A-AS1 expression was significantly higher in HCC cell lines (7721 and Huh7) than that in normal hepatocyte cell line L02 under normal culture condition. However, under nutrient-deficient condition, HIF1A-AS1 expression was significantly increased in both HCC and normal hepatocyte cell lines and was increased with the prolongation of nutrient-free culture. Inhibition of HIF1A-AS1 promoted starvation-induced HCC cell apoptosis. Furthermore, inhibition of HIF1A-AS1 could also reduce starvation-induced HCC cell autophagy. The expression of HIF-1 alpha and phosphorylated mTOR was significantly decreased in HCC cells after HIF1A-AS1 inhibition.ConclusionsHIF1A-AS1, overexpressed in HCC and associated with HCC prognosis, could regulate starvation-induced HCC cell apoptosis by reducing HIF-1 alpha /mTOR-mediated autophagy, promoting HCC cell progression.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据