Multi-instrumental approach to unravel molecular mechanisms of natural bioactive compounds: Case studies for flavonoids

Flavonoids are natural compounds defined as plant secondary metabolites with various beneficial properties for human health that make them perspective drug candidates. However, research in this area should be deepened to collect reasonable amount of data to suggest biochemical mechanism of action that can be a key for evaluation of drug efficacy, side effects. In addition, from analytical point of view, analyte-receptor interaction is an interesting object for study as it includes challenges in sample preparation and analytical measurements. Thus, there is a need in modern instrumental analytical techniques for study of drug-receptor interactions. Combination of instrumental techniques represents multi-instrumental approach and provides an extended set of data to suggest mechanism and to evaluate changes in initial biological activity caused by interaction. Current review is focused on advances and limitations of multi-instrumental approach and analytical techniques for study of flavonoid-receptor interactions and insights into molecular mechanisms of flavonoids. (C) 2020 Elsevier B.V. All rights reserved.