Article
Reproductive Biology
Meng Zhang, Sheng Zhang, Yanhui Zhai, Yu Han, Rong Huang, Xinglan An, Xiangpeng Dai, Ziyi Li
Summary: Inhibition of RNA m(6)A with cycloleucine (CL) impairs porcine oocyte maturation and early embryonic development, resulting in compromised cumulus expansion, increased ROS production in mitochondria, and halted embryo development. Additionally, CL treatment affects the expression of metabolism-related genes and histone modifications in blastocysts.
Article
Biochemistry & Molecular Biology
Mengya Zhang, Xin Wang, Qiuchen Liu, Yelian Yan, Changzhi Xu, Zhihua Zhu, Sucheng Wu, Xiaoyu Zuo, Yanfeng Zong, Chunxiao Li, Yunhai Zhang, Zubing Cao
Summary: 3-MC exposure has toxic effects on the maturation of oocytes and development of embryos. It inhibits cumulus expansion and polar body extrusion, reduces cleavage and blastocyst rates, induces spindle abnormalities and chromosomal misalignments, and alters the levels of mitochondria, cortical granules, acetylated alpha-Tubulin, reactive oxygen species, DNA damage, and apoptosis. Abnormal gene expression related to cumulus expansion and apoptosis is also observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Yu Wu, Mo Li, Mo Yang
Summary: Post-translational modifications such as phosphorylation, ubiquitination, SUMOylation, and PARylation play crucial roles in regulating mammalian oocyte maturation and embryo development by modulating processes like chromosome condensation, protein degradation, and cell cycle progression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
De-Cai Xiang, Bao-Yu Jia, Xiang-Wei Fu, Jian-Xiong Guo, Qiong-Hua Hong, Guo-Bo Quan, Guo-Quan Wu
Summary: The study demonstrated that Astaxanthin (Ax) significantly improved the survival rate of vitrified oocytes, promoted blastocyst yield, reduced reactive oxygen species generation, and increased glutathione level. Ax also decreased cathepsin B activity, increased lysosome fluorescence intensity, enhanced mitochondrial activity, and restored expression of specific genes in vitrified oocytes, ultimately improving developmental competence.
Review
Biochemistry & Molecular Biology
Michal Dvoran, Lucie Nemcova, Jaroslav Kalous
Summary: This review describes the epigenetic changes determined by assisted reproduction technology (ART) in oogenesis, early embryogenesis, and fetal development. It emphasizes the impact of environmental and lifestyle factors on post-ART embryo transfer and fetal development, and compares the epigenetic parallel between animal models and human ART to illustrate possible mechanisms for infertility management.
Article
Cell Biology
Lili Guo, Yongda Zhao, Yanjun Huan
Summary: The study showed that chlorpyrifos had toxic effects on porcine oocytes, including delaying meiotic progression, decreasing polar body extrusion, disrupting spindle assembly and causing DNA damage, as well as impairing cytoplasmic maturation. Pterostilbene was able to effectively alleviate the damage caused by chlorpyrifos and improve oocyte maturation and embryonic development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Sicong Yu, Lepeng Gao, Chang Zhang, Yumeng Wang, Hainan Lan, Qianran Chu, Suo Li, Xin Zheng
Summary: This study demonstrated that glycine can improve ER stress induced by TG treatment and enhance oocyte quality and developmental potential by regulating intracellular calcium levels, mitochondrial function, and ER stress response pathways. Glycine supplementation alleviated ER stress, reduced ROS levels, maintained mitochondrial function, and restored calcium equilibrium in porcine oocytes exposed to ER stress inducers. Glycine also modulated apoptosis-related factors and gene expression, suggesting its potential for enhancing oocyte maturation in vitro.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Kui Sun, Yunqiao Han, Jingzhen Li, Shanshan Yu, Yangjun Zhang, Jamas Reilly, Jiayi Tu, Danna Jia, Xiang Chen, Hualei Hu, Mengmeng Ren, Pei Li, Jiong Luo, Xiang Ren, Xianqin Zhang, Xinhua Shu, Fei Liu, Mugen Liu, Zhaohui Tang
Summary: This study demonstrates the necessary role of DHX38 in retinal development and reveals a novel regulatory axis involving R-loop, replication stress, and DNA damage.
Review
Plant Sciences
Subodh Verma, Venkata Pardha Saradhi Attuluri, Helene S. Robert
Summary: Transcription factors play important regulatory roles in different stages of embryo development, including embryonic patterning and seed maturation. Specific transcription factor families, such as WOX, HD-ZIP III, ARF, and CUC, have distinct functions in embryonic patterning, while LEC1, ABI3, FUS3, and LEC2 are considered the master regulators of seed maturation. Genes associated with these transcription factors have been identified using genomics approaches and chromatin immunoprecipitation.
Article
Biochemistry & Molecular Biology
Wentao Li, Ziyi Liu, Peng Wang, Ran Di, Xiangyu Wang, Yufang Liu, Mingxing Chu
Summary: In this study, the researchers identified the core promoter region of BMP15 and its DNA binding motif. They also investigated the effects of BMP15 and the transcription factor RUNX1 on oocyte maturation and the TGF-beta signaling pathway. The results showed that overexpression of BMP15 and RUNX1 positively regulated oocyte maturation and the expression of BMPR1B and ALK5, while interference with BMP15 and RUNX1 had the opposite effects. This study provides a theoretical basis for regulating mammalian oocyte maturation through the BMP15/TGF-beta signaling pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Fisheries
Robie Allan Bombardelli, Juliana Kasper Mewes, Alexandre Henrique Buzzi, Ahiana Cassia de Oliveira Pedreira, Mirna Adriane Syperreck, Anna Cristina Strieder Dalmaso, Tais Voelkl Chagas, Rogerio Jose Chiella, Fabio Meurer
Summary: The study evaluated the effects of feeding Nile tilapia broodstock with rations containing different levels of crude glycerin, finding that crude glycerin in the feed can have negative impacts on females by decreasing egg production and hatching rates.
Article
Cell Biology
Song-Hee Lee, Ming-Hong Sun, Dongjie Zhou, Wen-Jie Jiang, Xiao-Han Li, Geun Heo, Xiang-Shun Cui
Summary: Exposure to high temperature has been found to negatively affect the development of porcine oocytes. It induces mitochondrial dysfunction, ER stress, abnormal Golgi apparatus distribution, and lysosomal damage, leading to the failure of meiotic maturation in the oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Zhaoyue Men, Meng Cao, Yuechan Gong, Lun Hua, Ruihao Zhang, Xin Zhu, Lianchao Tang, Xuemei Jiang, Shengyu Xu, Jian Li, Lianqiang Che, Yan Lin, Bin Feng, Zhengfeng Fang, De Wu, Yong Zhuo
Summary: The study found that increasing dietary fiber intake can promote oocyte maturation and uterine development in gilts, possibly through regulation of microbial communities and changes in metabolites.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Reproductive Biology
Yue Wang, Chun-Hua Xing, Shun Chen, Shao-Chen Sun
Summary: The study showed that ZEN exposure disrupted organelle function in porcine oocyte meiotic maturation, affecting mitochondria, endoplasmic reticulum, Golgi apparatus, and lysosome.
Article
Agriculture, Dairy & Animal Science
Juan P. Cabeza, Juan Camera, Olinda Briski, Minerva Yauri Felipe, Daniel F. Salamone, Andres Gambini
Summary: This study investigates the use of a novel zinc chelator for activating bovine and porcine oocytes. The results show that the zinc chelator can successfully induce blastocyst development in both species, although developmental rates and embryo quality are compromised in bovine compared to calcium-induced embryos. On the contrary, the new concentration and incubation time of the zinc chelator improve developmental rates in porcine without affecting embryo quality. These findings contribute to understanding the role of zinc in oocyte activation and preimplantation embryo development across different mammalian species.
Article
Developmental Biology
Reza K. Oqani, Tao Lin, Jae Eun Lee, So Yeon Kim, Soo Jin Sa, Je Seok Woo, Dong Il Jin
Article
Multidisciplinary Sciences
Reza K. Oqani, Tao Lin, Jae Eun Lee, Ki Myung Choi, Hyun Young Shin, Dong Il Jin
Article
Reproductive Biology
Tao Lin, Jae Eun Lee, Reza K. Oqani, So Yeon Kim, Eun Seok Cho, Yong Dae Jeong, Jun Jong Baek, Dong Il Jin
REPRODUCTIVE BIOLOGY
(2016)
Article
Reproductive Biology
Tao Lin, Reza K. Oqani, Jae Eun Lee, Hyun Young Shin, Dong Ii Jin
Article
Reproductive Biology
Reza K. Oqani, Tao Lin, Jae Eun Lee, So Yeon Kim, Jung Won Kang, Dong Il Jin
REPRODUCTIVE BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Yun Fei Diao, Tao Lin, Xiaoxia Li, Reza K. Oqani, Jae Eun Lee, So Yeon Kim, Dong Il Jin
Article
Reproductive Biology
Tao Lin, Reza K. Oqani, Jae Eun Lee, Jeong Won Kang, So Yeon Kim, Eun Seok Cho, Yong Dae Jeong, Jun Jong Baek, Dong Il Jin
REPRODUCTIVE TOXICOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Tao Lin, Jae Eun Lee, Jeong Won Kang, Reza K. Oqani, Eun Seok Cho, Seong Bok Kim, Dong Il Jin
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2018)
Article
Agriculture, Dairy & Animal Science
Joo Bin Lee, Min Gu Lee, Tao Lin, Hyeon Yeong Shin, Jae Eun Lee, Jung Won Kang, Dong-Il Jin
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
(2019)
Review
Biochemistry & Molecular Biology
Tao Lin, Jae Eun Lee, Jung Won Kang, Hyeon Yeong Shin, Ju Bin Lee, Dong Il Jin
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Agriculture, Dairy & Animal Science
Tao Lin, Jae Eun Lee, Hyeon Yeong Shin, Joo Bin Lee, So Yeon Kim, Dong Il Jin
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2019)
Article
Multidisciplinary Sciences
Reza K. Oqani, Tao Lin, Jae Eun Lee, Jeong Won Kang, Hyun Young Shin, Dong Il Jin
SCIENTIFIC REPORTS
(2019)
Article
Agriculture, Dairy & Animal Science
Tao Lin, Jae Eun Lee, Reza K. Ogani, So Yeon Kim, Eun Seok Cho, Yong Dae Jeong, Jun Jong Bael, Dong Il Jin
ANIMAL REPRODUCTION SCIENCE
(2017)
Article
Agriculture, Dairy & Animal Science
Tao Lin, Ling Sun, Jae Eun Lee, So Yeon Kim, Dong Il Jin
Summary: DNA damage increases and repair ability decreases in aged porcine oocytes, affecting cell growth and embryo quality.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2021)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.