Article
Chemistry, Multidisciplinary
Wenyan Nie, Kui He, Zhipeng Zhao, Xiaoxi Luo, Jinbin Liu
Summary: We developed a facile in situ strategy to precisely control DNA aptamer valences on the surface of luminescent gold nanoparticles (AuNPs) with emission in the second near-infrared window. We further demonstrated that not only the tumor-targeting efficiencies but also the subcellular transport of AuNPs show precise valence dependence.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Lingshan Gong, Kui He, Jinbin Liu
Summary: The study demonstrates the concentration-dependent subcellular distribution of near-infrared-emitting gold nanoparticles. At high concentrations, the nanoparticles exhibit strong membrane binding, while at low concentrations, they are more targeted towards mitochondria.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Pharmacology & Pharmacy
Raffaella Daniele, Chiara Brazzale, Busra Arpac, Francesco Tognetti, Cristiano Pesce, Alessio Malfanti, Edward Sayers, Francesca Mastrotto, Arwyn T. Jones, Stefano Salmaso, Paolo Caliceti
Summary: The surface density of targeting agents affects the cell interaction, mechanism of cell entry, and intracellular fate of surface decorated nanoparticles. Increasing folic acid density on folate-targeted gold nanoparticles has been shown to enhance their internalization and trafficking to lysosomes in folate receptor overexpressing cells. Higher folic acid density induces more efficient particle internalization and predominantly internalizes the particles by a clathrin-independent process.
Article
Chemistry, Multidisciplinary
Baoning Li, Yaping Wang, Michael Ho-Yeung Chan, Mei Pan, Yonguang Li, Vivian Wing-Wah Yam
Summary: By utilizing cationic organoplatinum(II) complexes with switchable luminescence properties, precise imaging of subcellular distribution within cells can be achieved, offering a new way to differentiate live and dead cells. This research lays the groundwork for potential subcellular detection assays.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Asier R. R. Muguruza, Alessandro di Maio, Nikolas J. J. Hodges, Jessica M. A. Blair, Zoe Pikramenou
Summary: In this study, a novel hybrid silica nanoparticle system was designed, which carried membrane targeting groups and encapsulated antibiotics along with a ruthenium luminescent tracking agent for optical detection of nanoparticle delivery in bacterial cells. The hybrid system successfully delivered vancomycin and exhibited efficacy against various Gram-negative bacterial species. The penetration of nanoparticles in bacterial cells was confirmed via the luminescence of the ruthenium signal. These findings demonstrate that nanoparticles modified with aminopolycarboxylate chelating groups can serve as an effective delivery system for inhibiting bacterial growth in species where molecular antibiotics are ineffective. This design provides a new platform for delivering antibiotics that cannot penetrate the bacterial membrane alone.
NANOSCALE ADVANCES
(2023)
Article
Biochemical Research Methods
Mengmeng Liu, Fei Wang, Xueli Zhang, Xiuhai Mao, Lihua Wang, Yang Tian, Chunhai Fan, Qian Li
Summary: This method provides a step-by-step correlative imaging approach to investigate plasmonic NPs of different aggregation states in single cells. By using dark-field microscopy, fluorescence microscopy, and scanning electron microscopy, the clustering states of SNAs in a single cell can be efficiently distinguished and tracked in real time within 20 seconds. The entire procedure can be completed in 3-5 days and requires standard imaging and data analysis skills and equipment.
Article
Chemistry, Analytical
Shihua Li, Nangen Yang, Qiuping Ma, Shijie Li, Shufen Tong, Jiewei Luo, Xiaorong Song, Huanghao Yang
Summary: This study investigates the oxidation responsiveness of near-infrared II (NIR-II) luminescent AuNCs and finds that AuNCs capped with different ligands have different oxidation properties in the presence of peroxynitrite anions (ONOO-). N-acetylcysteine-capped AuNCs are more easily oxidized and aggregate into large-sized assemblies, while macromolecular mercapto-beta-cyclodextrin-capped AuNCs do not show this behavior. Furthermore, N-acetylcysteine-capped AuNCs are demonstrated to be effective for sensitive NIR-II PL imaging of mice acute kidney injury (AKI), thanks to the synergistic effect of in situ AuNC aggregation and decreased glomerular filtration rate (GFR) in the injured kidney, surpassing methods solely based on decreased GFR.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yan Tan, Jianxing Zhou, Xiaotong Xing, Junren Wang, Jinkun Huang, Huiyu Liu, Jiajie Chen, Mingjie Dong, Qin Xiang, Haifeng Dong, Xueji Zhang
Summary: In this study, the controllable self-assembly of DNA-linked gold nanoparticles (AuNPs) for in vivo biomedical applications was achieved by using programmable DNA tetrahedral structures. Three different sizes of AuNPs (20nm, 10nm, and 5nm) were organized and positioned using this method. The density and arrangement of the satellite AuNPs around the core AuNPs were controlled by tuning the size and amount of DNA tetrahedron functionalized on the core AuNPs, resulting in strong electromagnetic field enhancement. With the conjugation of Raman molecules, strong surface-enhanced Raman scattering photoacoustic imaging signals were generated, allowing for the imaging of microRNA-21 and tumor tissues in vivo. This study provides an efficient strategy for building precision plasmonic superstructures in plasmonic-based bioanalysis and imaging.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Emily Xi Tan, Yichao Chen, Yih Hong Lee, Yong Xiang Leong, Shi Xuan Leong, Chelsea Violita Stanley, Chi Seng Pun, Xing Yi Ling
Summary: Determining the size and size distribution of nanoparticles is crucial for understanding their properties and applications. This study introduces a plasmonic-domain-driven feature engineering combined with machine learning approach to accurately predict the parameters of nanoparticle ensembles. The method achieves significantly lower prediction errors than previous approaches and demonstrates the ability to overcome challenges such as size anisotropy and small sample sizes. In addition, the study showcases the inverse prediction capabilities, using size and size distribution to generate spectra that closely match experimental data.
NANOSCALE HORIZONS
(2022)
Article
Multidisciplinary Sciences
Christian Koernig, Theresa Staufer, Oliver Schmutzler, Tanja Bedke, Andres Machicote, Beibei Liu, Yang Liu, Elisabetta Gargioni, Neus Feliu, Wolfgang J. Parak, Samuel Huber, Florian Gruener
Summary: X-ray fluorescence imaging is a non-invasive method for detecting small amounts of elements, especially nanoparticles in cells and tissues. However, its sensitivity decreases for thicker objects due to increased background caused by Compton scattering. Reducing the detection limit would allow tracking of labeled cells and provide new insights in medicine.
SCIENTIFIC REPORTS
(2022)
Article
Optics
Yao Li, Chengbing Qin, Yunrui Song, Hongyan Yan, Shuangping Han, Haitao Zhou, Aoni Wei, Guofeng Zhang, Ruiyun Chen, Jianyong Hu, Mingyong Jing, Liantuan Xiao, Suotang Jia
Summary: A double-pulse TPPL scheme with controlled phase differences was developed to significantly improve the signal-to-noise ratio of TPPL imaging, allowing for enhanced imaging quality while reducing excitation powers.
Article
Chemistry, Analytical
Xi Chen, Zhongwei Jiang, Ling Liang, Yuan Fang Li, Cheng Zhi Huang, Peng Fei Gao
Summary: By utilizing the Au NBPs@ZIF-8 nanoprobe, the fluctuation of ATP level in living cells can be monitored with high sensitivity, selectivity, and biocompatibility. This method demonstrates a good linear relationship, low detection limit, and excellent selectivity, allowing for effective observation of intracellular ATP changes.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Qamar A. Alshammari, Rajasekharreddy Pala, Ayan K. Barui, Saud O. Alshammari, Andromeda M. Nauli, Nir Katzir, Ashraf M. Mohieldin, Surya M. Nauli
Summary: This study integrates spectral imaging technology with AI-driven analysis to achieve high-resolution detection and identification of nanoparticles. By simultaneously analyzing 45 spectral points, the core and shell of nanoparticles can be distinguished, providing a new approach for nanoparticle localization and identification in biological samples.
Article
Multidisciplinary Sciences
Xavier Rovira-Clave, Sizun Jiang, Yunhao Bai, Bokai Zhu, Graham Barlow, Salil Bhate, Ahmet F. Coskun, Guojun Han, Chin-Min Kimmy Ho, Chuck Hitzman, Shih-Yu Chen, Felice-Alessio Bava, Garry P. Nolan
Summary: This study introduces a high-definition multiplex ion beam imaging technology that allows simultaneous visualization of multiple biomolecules and their ligands or small molecules in cells. The subcellular localization of the chemotherapy drug cisplatin was found to be related to chromatin regions, with surviving cells after multi-drug treatment showing selective exclusion of cisplatin from the nucleus, potentially modulating resistance to chemotherapy treatment.
NATURE COMMUNICATIONS
(2021)
Article
Engineering, Environmental
Nanyan Weng, Paul Guagliardo, Haibo Jiang, Wen-Xiong Wang
Summary: This study investigated the distribution and accumulation of manganese in oyster oocytes and sperm cells, revealing significant differences in subcellular localization and accumulation capacity between the two cell types. Manganese was found to mainly accumulate in cortical granules of oocytes, while it was primarily distributed in the nucleus of sperm cells, indicating sex differentiation in susceptibility to manganese stress. This highlights the importance of considering gender differences in future biomonitoring and ecotoxicological studies of manganese in marine bivalves.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Lingshan Gong, Kui He, Jinbin Liu
Summary: The study demonstrates the concentration-dependent subcellular distribution of near-infrared-emitting gold nanoparticles. At high concentrations, the nanoparticles exhibit strong membrane binding, while at low concentrations, they are more targeted towards mitochondria.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Yue Tan, Miaona Chen, Huarui Chen, Juefei Wu, Jinbin Liu
Summary: This study introduces ultrasmall renal-clearable luminescent gold nanoparticles with pH-induced charge reversal and aggregation properties, which enhance ultrasound contrast in injured kidneys through increased tubular reabsorption and in situ aggregation. The tuning elimination pathway of these nanoparticles provides a synergistical fluorescence and ultrasound imaging strategy for diagnosing early kidney injury with precise anatomical information.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Kui He, Yue Tan, Zhipeng Zhao, Huarui Chen, Jinbin Liu
Summary: The study achieved controllable weak anchoring sites of luminescent gold nanoparticles and demonstrated the importance of N-Au and COO-Au weak anchoring sites in the behavior of AuNPs, opening up new possibilities for advances in the field of nanomedicine.
Review
Chemistry, Multidisciplinary
Xiaoxi Luo, Jinbin Liu
Summary: In the past decade, ultrasmall luminescent metal nanoparticles have made rapid progress in addressing challenges in healthcare due to their excellent properties. Surface engineering strategies of ULMNPs, including enhancing disease targeting abilities and self-assembly for multifunctional structures, have significantly advanced biological applications from bioimaging to theranostics. Further research on ratiometric ULMNPs for improved imaging stability in complex biological environments, along with the exploration of phototoxicity and future perspectives, aim to accelerate their clinical applications.
Article
Radiology, Nuclear Medicine & Medical Imaging
Xuewei Liu, Rongkang Gao, Chiyun Chen, Xiaobo Li, Chen Yu, Yejia Chen, Hongbin Liang, Min Xiao, Lei Dai, Shifeng Qiu, Xiaoyu Xin, Liang Song, Jinbin Liu, Chengbo Liu, Jiancheng Xiu
Summary: In this study, noninvasive assessment of alpha v beta 3-integrin expression in atherosclerotic lesions of varying degrees of severity was performed using a customed photoacoustic (PA) computed tomography (PACT)/ultrasound (US) system. The results showed that alpha v beta 3-integrin is an effective indicator of plaque instability, and noninvasive PACT/US molecular imaging assessment of alpha v beta 3-integrin holds promise in detecting high-risk plaques.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Article
Chemistry, Multidisciplinary
Kai Zhou, Wei Cai, Yue Tan, Zhipeng Zhao, Jinbin Liu
Summary: By manipulating the siloxane bridge crosslinking states, water-soluble nanoassemblies with disassembly-induced emission enhancement (DIEE) were successfully fabricated. The disassembled nanoassemblies showed more than 6-fold enhancement in emission and improved renal clearance efficiency, opening up new possibilities for designing highly emissive renal-clearable nanostructures.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Yue Tan, Wei Cai, Caiming Luo, Jiahao Tang, Ryan T. K. Kwok, Jacky W. Y. Lam, Jianwei Sun, Jinbin Liu, Ben Zhong Tang
Summary: By designing luminescent gold-silver bimetallic nanoparticles that can be rapidly transformed in the hepatic sinusoidal microenvironment, liver sequestration can be avoided, phagocytosis by mononuclear phagocyte system cells can be enhanced, rapid release can be promoted, renal clearance can be improved, and disease targeting can be increased.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Bing Tang, Wenle Xia, Wei Cai, Jinbin Liu
Summary: In this study, luminescent AuNPs with redshifted emission and good biocompatibility were successfully constructed using an amphiphilic block copolymer template. The strategy involved the formation of uniform clusters of NIR-II AuNPs inside the hydrophobic cores of ABC micelles and the generation of rigid NIR-II AuNPs as ABC unimers on the surface, enabling enhanced biological interactions.
Article
Chemistry, Multidisciplinary
Wei Cai, Yue Tan, Kui He, Bing Tang, Jinbin Liu
Summary: A precise control of nanoparticle size in the sub-nanometer regime is crucial for understanding nano-bio interactions and advancing nanomedicine. This study presents a simple Mn2+-guided centrifugation strategy to synthesize large-scale ultrasmall AuNPs with precise size gradient at the sub-nanometer regime. The researchers discovered that [Mn(OH)](+) nanoparticles, particularly Mn-0@[Mn(OH)](+), can selectively interact with larger AuNPs to form aggregates, leading to the efficient synthesis of water-soluble atomically precise Au-25 within a short time. Moreover, the study demonstrates the significant impact of sub-nanometer size differences on non-specific phagocytosis, elimination rate, and nanotoxicology of AuNPs in macrophages.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Zhipeng Zhao, Huarui Chen, Kui He, Jincheng Lin, Wei Cai, Yidan Sun, Jinbin Liu
Summary: This study develops a facile in vivo ligand exchange strategy to achieve renal-clearable activatable luminescent gold nanoparticles (AuNPs), which are independent of biomarkers for sensitive and long-time imaging of early kidney injury.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Kui He, Yuan-Fu Ding, Zhipeng Zhao, Ben Liu, Wenyan Nie, Xiaoxi Luo, Hua-Zhong Yu, Jinbin Liu, Ruibing Wang
Summary: This study reports a strategy to achieve liver or kidney-specific delivery by altering the surface chemistry of nanocarriers. The results demonstrate that ultrasmall nanocarriers can effectively deliver drugs to injured kidneys and achieve improved therapeutic outcomes. This organ-specific delivery strategy provides a new approach for improving drug delivery efficiency and personalized treatments.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Wenyan Nie, Kui He, Zhipeng Zhao, Xiaoxi Luo, Jinbin Liu
Summary: We developed a facile in situ strategy to precisely control DNA aptamer valences on the surface of luminescent gold nanoparticles (AuNPs) with emission in the second near-infrared window. We further demonstrated that not only the tumor-targeting efficiencies but also the subcellular transport of AuNPs show precise valence dependence.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Yue Tan, Di Huang, Caiming Luo, Jiahao Tang, Ryan T. K. Kwok, Jacky W. Y. Lam, Jianwei Sun, Jinbin Liu, Ben Zhong Tang
Summary: In this study, clearable mercaptosuccinic acid (MSA) coated Au-Ag bimetallic nanoparticles (NPs) were designed to enhance reactive oxygen species (ROS) production. The strong COO-Ag coordination bonds between MSA and Ag atoms on the NPs restricted intrananoparticle motions, leading to enhanced ROS generation. The bimetallic NPs also exhibited pH-responsive self-assembly capability, further boosting ROS production and showing high tumor targeting efficiency and effective cancer cell destruction.
Article
Chemistry, Multidisciplinary
Jiayi Zhu, Zhipeng Zhao, Huarui Chen, Xinglin Chen, Jinbin Liu
Summary: This study demonstrates that renal-clearable nanomedicines show a more sensitive response to injection dose and have improved tumor-targeting efficiency compared to nonrenal-clearable nanoparticles.
Article
Chemistry, Multidisciplinary
Yue Tan, Wei Cai, Caiming Luo, Jiahao Tang, Ryan T. K. Kwok, Jacky W. Y. Lam, Jianwei Sun, Jinbin Liu, Ben Zhong Tang
Summary: The rapid transformation of luminescent gold-silver bimetallic nanoparticles in the hepatic sinusoidal microenvironment can help avoid MPS phagocytosis, promote fast release from the liver, prolong blood circulation, enhance renal clearance, and increase disease targeting, turning liver sequestration into a beneficial storage mechanism for nanomedicines.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)