期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 311, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.127891
关键词
Carbon dots; Long-wavelength excitation; Nucleolus; Chromosome; Cell cycle process
资金
- National Key R&D Program of China [2018YFC0910600]
- National Natural Science Foundation of China [61775145, 61525503, 61620106016, 61835009, 81727804, 31771584, 61975132]
- China Postdoctoral Science Foundation [2019M650211]
- Project of Department of Education of Guangdong Province [2015KGJHZ002/2016KCXTD007]
- Shenzhen Basic Research Project [JCYJ20170412110212234, JCYJ20170412105003520, JCYJ20170818100153423]
- Natural Science Foundation of SZU [2017000193, 2019108]
Cell-cycle targeting probes are crucial for observing and understanding how different drugs affect the cell stages. Here, we designed and prepared fluorescent F and N co-doped carbon dots (FNCDs) with a long-wavelength excitation, excellent optical stability, high fluorescence quantum yield (57 %), good water-solubility, low toxicity, and anti-photobleaching. The FNCDs have a positively charged surface, which enables easy absorption into DNA and RNA in vitro. For living cells, FNCDs can disperse the 4T1 cell via endocytosis pathways, and label both the nucleolus and chromosome. Due to long-wavelength excitation and excellent anti-photobleaching, we used the FNCDs as probes to directly monitor the cell division of living 4T1 cells, and we obtained the whole progress of cell division for the same cell. The experimental data showed the different chromosome morphologies of living cells in the various stages of cell division. Because of the low cost of raw material and excellent optical stability, FNCDs will hopefully replace dyes as nuclear and chromosome probes. This may open new ways to apply FNCDs in modern biology and cytology.
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