期刊
RNA BIOLOGY
卷 17, 期 10, 页码 1454-1471出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2020.1775395
关键词
20-hydroxyecdysone; ecdysone-signalling pathway; microRNA; insect; Bombyx mori; Drosophila melanogaster
资金
- National Natural Science Foundation of China [31571334, 31071136]
- Basic and Frontier Research Project of Chongqing [cstc2014jcyjA00025]
20-Hydroxyecdysone (20-HE) plays essential roles in coordinating developmental transitions of insects through responsive protein-coding genes and microRNAs (miRNAs). The involvement of single miRNAs in the ecdysone-signalling pathways has been extensively explored, but the interplay between ecdysone and the majority of miRNAs still remains largely unknown. Here, by small RNA sequencing, we systematically investigated the genome-wide responses of miRNAs to 20-HE in the embryogenic cell lines ofBombyx moriandDrosophila melanogaster. Over 60 and 70 20-HE-responsive miRNAs were identified in the BmE cell line and S2 cell line, respectively. The response of miRNAs to ecdysone exhibited a time-dependent pattern, and the response intensity increased with extending exposure to 20-HE. The relationship between ecdysone and the miRNAs was further explored through knockdown of ecdysone-signalling pathway genes. Specifically, ecdysone regulated the cluster miR-275 and miR-305 through the coordination ofBmEcR-Band downstreamBmE75B, and the interaction betweenBmEcRand miR-275 cluster was strengthened by the feedback regulation ofBmE75B. Ecdysone induced miR-275-3p and miR-305-5p through the ecdysone response effectors (EcREs) at the upstream of the pre-miR-275 cluster. Overall, the results might help us further understand the relationship between ecdysone signalling pathways and small RNAs in the development and metamorphosis of insects.
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