Article
Multidisciplinary Sciences
Asmaa Abdella, Samah Ramadan, Ragaa A. Hamouda, Amna A. Saddiq, Nuha M. Alhazmi, Mahmoud A. Al-Saman
Summary: Paecilomyces variotii xylanase exhibited optimal activity at high temperature and low acidity, with significantly increased antioxidant activity towards xylo-oligosaccharides. Its characteristics and kinetic parameters indicate its potential effectiveness in xylan hydrolysis and other industrial applications.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Do Thi Tuyen, Nguyen Tien Cuong, Nguyen Sy le Thanh, Nguyen Thi Thao, Le Thanh Hoang, Nguyen Thi Hien Trang, Nguyen Thi Trung, Dao Thi Mai Anh
Summary: The study focused on engineering recombinant Aspergillus niger to produce highly active xylanase G2, with successfully cloning, amplification, and expression of the gene encoding this enzyme. The purified enzyme showed specific activity and stability under certain temperature and pH conditions, with enhancements in activity observed in the presence of Tween 80. However, the enzyme's function was impacted by certain substances and completely inhibited by Hg2+. (Summary in English)
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Microbiology
Qinghua Li, Jinchang Lu, Guoqiang Zhang, Jingwen Zhou, Jianghua Li, Guocheng Du, Jian Chen
Summary: In this study, high-efficiency transformation of Aspergillus oryzae was achieved by optimizing the preparation conditions of protoplasts, and random editing of single and double genes using the CRISPR/Cas9 system reached 37.6% and 19.8% efficiency, respectively. With the aid of selection markers, such as color or resistance, the editing efficiency of single and double genes could reach 100%. The development of this system improves the efficiency of transformation and multiplexed genome editing for A. oryzae, providing a practical method for developing a high-efficiency expression system for heterologous proteins.
Article
Chemistry, Physical
Soni Tiwari, Ranjan Singh, Janardan Yadav, Rajeeva Gaur, Anurag Singh, Jay Shankar Yadav, Prabhash Kumar Pandey, Santosh Kumar Yadav, Jaya Prajapati, Pukhrambam Helena, Jayant Dewangan, Farrukh Jamal
Summary: In this study, an alkali-thermo-tolerant xylanase from Bacillus paramycoides that is tolerant to organic solvents was successfully produced. The enzyme showed improved activity and stability in the presence of certain organic solvents. This enzyme has potential applications in biomass conversion, fuel production, and other industrial processes, as well as in bioethanol production, alcoholic beverage amplification, and the fermenting industry.
Article
Multidisciplinary Sciences
Gabriella Cavazzini Pavarina, Eliana Gertrudes de Macedo Lemos, Natalia Sarmanho Monteiro Lima, Joao Martins Pizauro Jr
Summary: Researchers identified a new bifunctional enzyme from the Nellore cattle rumen microbiota, which is stable and tolerant to various conditions and suitable for industrial environments. The enzyme shows potential for improving the degradation efficiency of heteroxylans and could be applied in lignocellulosic biomass processing and animal feed additives.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yuzhen Li, Huanxin Zhang, Ziming Chen, Junxia Fan, Tianming Chen, Yi Xiao, Junyin Jie, Bin Zeng, Zhe Zhang
Summary: This study identified a novel functional gene, Aokap2, that is involved in the growth and production of kojic acid in A. oryzae. Overexpression of Aokap2 increased kojic acid production by affecting the expression of laeA and kojA.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Agronomy
Congying Wang, Shen Chen, Aiqing Feng, Jing Su, Wenjuan Wang, Jinqi Feng, Bing Chen, Meiying Zhang, Jianyuan Yang, Liexian Zeng, Xiaoyuan Zhu
Summary: The research revealed that the rice resistance gene Xa7 is an orphan gene encoding a novel protein that triggers resistance reaction by perceiving AvrXa7. Additionally, it was found that Xa7 is absent in most cultivated rice varieties, but highly homologs of XA7 were identified in wild grasses such as Leersia perrieri.
Article
Biotechnology & Applied Microbiology
Girisha Malhotra, Shilpa S. Chapadgaonkar
Summary: The xylanase used in this study showed high deinking potential, resulting in a 50% reduction in the usage of chemical bleach. The comparison of FTIR spectra indicated that the xylanase pre-treatment process caused negligible damage to the fiber strength compared to the chemical bleach process.
JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Isaac A. Olopoda, Olusola T. Lawal, Oluwasegun Omotoyinbo, Adejoke N. Kolawole, David M. Sanni
Summary: The study investigates the efficiency and properties of immobilized and free xylanase from Aspergillus awamori AFE1 for biodegradation. The results show that the xylanase exhibits suitable characteristics for industrial and biotechnological applications.
PROCESS BIOCHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Hiroyuki Yamada, Shoko Kubo, Yuika Kunishige, Hotaru Azuma, Yuka Kotani, Satoshi Handa, Masami Nakazawa, Mitsuhiro Ueda, Yoshinori Hasegawa, Tatsuji Sakamoto
Summary: The study investigated pectin methylesterase (PME) enzymes in Aspergillus oryzae, revealing differences in specific activity and substrate specificity among three different enzymes. The findings provide insight into the distribution of methoxy groups in pectin and offer potential for new applications in food manufacturing.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Andrey Valentinovich Zadorozhny, Viktor Sergeevich Ushakov, Alexei Sergeevich Rozanov, Natalia Vladimirovna Bogacheva, Valeria Nikolayevna Shlyakhtun, Mikhail Evgenyevich Voskoboev, Anton Vladimirovich Korzhuk, Vladislav Anatolevich Romancev, Svetlana Valerevna Bannikova, Irina Anatolyevna Mescheryakova, Egor Vladimirovich Antonov, Asya Rifhatovna Vasilieva, Elena Iurevna Pavlova, Danil Olegovich Chesnokov, Elizaveta Dmitrievna Shedko, Alla Viktorovna Bryanskaya, Denis Vladimirovich Bochkov, Tatiana Nikolayevna Goryachkovskaya, Sergey Evgenyevich Peltek
Summary: This study achieved efficient expression of the xAor xylanase gene in yeast and purified the enzyme with high activity. The results suggested that the purified enzyme can be directly applied in industrial applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Eiichiro Kan, Hiroya Tomita, Yohei Katsuyama, Jun-ichi Maruyama, Yasuji Koyama, Yasuo Ohnishi
Summary: The filamentous fungus Aspergillus oryzae has 27 putative iterative type I polyketide synthase (PKS) gene clusters, with unknown secondary metabolites. This study focused on eight clusters expressed at high levels, revealing the biosynthesis of 2,4'-dihydroxy-3'-methoxypropiophenone and its precursor, and the essential roles of PpsB and PpsA in their production. PpsC was also implicated in the conversion process from 3 to 1.
Article
Multidisciplinary Sciences
Steffy Angural, Indu Bala, Aditya Kumar, Deepak Kumar, Sunena Jassal, Naveen Gupta
Summary: The study demonstrates that co-culturing strains in solid-state fermentation can significantly increase the yield of xylanase and mannanase, providing a promising alternative for reducing chemical use in the paper industry. The enzyme cocktail produced shows great potential for pulp biobleaching, resulting in a substantial reduction of kappa number and improved brightness and whiteness of the paper.
Article
Microbiology
Jing Si, Hongfei Ma, Yongjia Cao, Baokai Cui, Yucheng Dai
Summary: This study introduces a valuable laccase, ThLacc-S, which efficiently metabolizes endocrine disrupting chemicals and has high specific activity. The enzyme maintains stability in a wide temperature range and pH range, making it a promising green catalyst for various biotechnological and industrial applications.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Vikas Thakur, Dharam Singh
Summary: This study reports a novel and robust GH9 processive endoglucanase beta-1,4-glucanase from Bacillus sp. PCH94 (EGase(BL)) with thermo-alkali stable properties. The enzyme shows dual activities and compatibility with commercial detergents, making it a potential bioresource for the detergent industry, in addition to its implications for the bioethanol industry.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Energy & Fuels
Nisha Bhardwaj, Bikash Kumar, Pradeep Verma
Summary: Microwave-assisted alkali salt and H3PO4 pretreatment of rice straw, followed by enzymatic hydrolysis, exhibited strategic structural changes in the rice straw. The maximum saccharification and ethanol yields were achieved using commercial and in-house enzyme cocktails.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biotechnology & Applied Microbiology
Komal Agrawal, Pradeep Verma
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2020)
Review
Agricultural Engineering
Bikash Kumar, Pradeep Verma
INDUSTRIAL CROPS AND PRODUCTS
(2020)
Article
Ecology
Saket Kashyap, Rachna Chandra, Bikash Kumar, Pradeep Verma
Summary: The study demonstrated the effective use of endophytic microbial strains isolated from plants growing in nickel-contaminated soil for nickel bioremediation. The isolated bacterial strains showed high nickel biosorption efficiency and plant growth-promoting activities, making them suitable for large-scale biosorption of nickel from electroplating industry effluent. Kinetic studies and adsorption isotherms further supported the nickel biosorption ability of the endophytic strains.
Review
Microbiology
Rahul K. Goswami, Komal Agrawal, Pradeep Verma
Summary: Wastewater containing nitrogen and phosphate can cause eutrophication and harm aquatic ecosystems. Microalgae like Picochlorum sp. have the potential to remove these pollutants and be used for biofuel and bioproduct formation.
JOURNAL OF BASIC MICROBIOLOGY
(2022)
Article
Thermodynamics
Bikash Kumar, Pradeep Verma
Summary: Life cycle assessment (LCA) is a widely used tool that provides data-driven analysis of environmental performances. By selecting appropriate feedstocks, technology, and energy sources, valuable insights can be gained during the policy design phase. In biorefineries, transportation and manufacturing contribute significantly to energy consumption, and electricity usage has a major impact on global warming potential.
ENERGY CONVERSION AND MANAGEMENT-X
(2021)
Review
Green & Sustainable Science & Technology
Rahul Kumar Goswami, Komal Agrawal, Pradeep Verma
Summary: Municipal wastewater or urban wastewater is generated from domestic water consumption and contains a significant amount of nutrients. Untreated wastewater can harm aquatic life and human health. Microalgae, with the capability of nutrient assimilation and biomass production, offer a potential solution for treating wastewater. However, advanced approaches are required to improve the sustainability and efficiency of microalgae-based wastewater treatment systems.
CLEAN-SOIL AIR WATER
(2022)
Article
Environmental Sciences
Rahul Kumar Goswami, Komal Agrawal, Sanjeet Mehariya, Pradeep Verma
Summary: Urbanization leads to a generation of huge wastewater, with diverse nutrient loads and toxic compounds affecting ecosystems and health. Microalgae-based biological treatment, particularly Tetraselmis sp., is effective in removing nutrients and heavy metals, with biomass potentially used for bioenergy products. Photobioreactor (PBR) systems can improve biomass production and nutrient removal efficiency, making it a sustainable approach for wastewater treatment.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Review
Microbiology
Sahil Kapoor, Meenakshi Singh, Atul Srivastava, Murthy Chavali, K. Chandrasekhar, Pradeep Verma
Summary: Microalgae-derived bioactive phenolic compounds (MBPCs) are essential for biopharmaceutical and nutraceutical industries due to their diverse bioactivities. The use of state-of-the-art green technologies and modern progress has accelerated the discovery of novel active pharmaceutical compounds.
JOURNAL OF BASIC MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
R. K. Goswami, K. Agrawal, M. P. Shah, P. Verma
Summary: Drinking water and wastewater contaminated with heavy metals pose a severe threat to the environment and living beings. Utilizing biological methods, such as microalgae, can effectively remove heavy metals. Microalgae have potential for environmental applications and heavy metal remediation due to their unique capabilities.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Azat Abdullatypov, Pavel Oskin, Veronika Fedina, Liubov Trubitsina, Sofiya Yakimovich, Ekaterina Shuvalova, Pradeep Verma, Tatyana Dyachkova, Olga Ponamoreva, Sergey Alferov, Ioannis V. Yentekakis, Ying Zheng
Summary: This study assessed several modifications of carbon nanotube-based nanomaterials for their applications in laccase electrodes and model biofuel cells. The modified MWCNTs were used as adapters for immobilizing laccase from Catenuloplanes japonicus VKM Ac-875 onto graphite rod and graphite paste electrodes. The electrochemical properties and potential practical applications of the electrodes and biofuel cells were investigated.
Review
Biotechnology & Applied Microbiology
Nisha Bhardwaj, Bikash Kumar, Komal Agrawal, Pradeep Verma
Summary: Cellulolytic enzymes have great potential in bioconversion processes and various industrial applications. Strategies are being developed to enhance their production efficiency, while immobilization techniques can improve enzyme reusability. The review also provides insights into the significant industrial applications of cellulase and its future potential.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Engineering, Environmental
Bikash Kumar, Komal Agrawal, Pradeep Verma
Summary: Anthropogenic activities have caused the accumulation of toxic waste materials in the environment, posing a threat to food and health security. Conventional methods for removing these wastes are costly and ineffective, leading to increased interest in microbe-based bioremediation systems, particularly microbial electrochemical systems (MES). MES utilize electroactive microbes to convert chemical energy to electrical energy, showing promise in the selective removal of toxic compounds from wastewater.
JOURNAL OF HAZARDOUS TOXIC AND RADIOACTIVE WASTE
(2021)
Article
Biochemical Research Methods
Juri Sakata, Toshifumi Tatsumi, Akira Sugiyama, Akihiro Shimizu, Yuya Inagaki, Hiroto Katoh, Takefumi Yamashita, Kazuki Takahashi, Sho Aki, Yudai Kaneko, Takeshi Kawamura, Mai Miura, Masazumi Ishii, Tsuyoshi Osawa, Toshiya Tanaka, Shumpei Ishikawa, Masanobu Tsukagoshi, Michael Chansler, Tatsuhiko Kodama, Motomu Kanai, Hidetoshi Tokuyama, Kenzo Yamatsugu
Summary: This study reports an expression, refolding, and purification method for antibody-mimetic drug conjugate (AMDC). The AMDC uses a variable heavy chain of heavy chain-only antibodies (VHHs) as the targeting protein and non-covalently binds to a potent DNA-alkylating drug. The AMDC shows strong cytotoxic effects on cancer cells.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yao Wang, Shaoting Weng, Yajie Tang, Sen Lin, Xiayue Liu, Wenhui Zhang, Gang Liu, Boomi Pandi, Yinrong Wu, Lei Ma, Lin Wang
Summary: In this study, a high yield of recombinant CD20 and claudin 18.2 proteins was achieved using an in vitro coupled transcription-translation system. The results showed that rituximab has a high affinity with CD20 protein. This study provides a novel concept for promoting the expression of multi-pass transmembrane proteins and lays the foundation for large-scale industrial production of membrane-associated drug targets.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xiaobing Chen, Zijuan Zou, Wei Li, Xu Dong, Yi Chen, Yan Lu, Mingyue Zhu, Mengsen Li, Bo Lin
Summary: In this study, the fusion protein ImI-AFP3, composed of alpha-Conotoxin ImI and human alpha fetoprotein domain 3 (AFP3), was found to inhibit the growth and migration of lung cancer cells and showed synergistic effects with the drug gefitinib. These findings suggest that ImI-AFP3 is a promising candidate for the development of anticancer drugs.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Angel Castillo-Corujo, Mirva J. Saaranen, Lloyd W. Ruddock
Summary: This study successfully expressed two Fabs antibodies in the cytoplasm of E. coli using the CyDisCo system, achieving high yields and biological activity under industrially relevant fermentation conditions.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Junling Guo, Zhongyi Cheng, Zhemin Zhou
Summary: This study focused on a halophilic archaeal nitrile hydratase (NHase) and found that it exhibited higher tolerance to substrates and products compared to NHases from other sources. The unique genetic structure of this highly stable archaeal NHase could provide a theoretical foundation for modifying and enhancing the industrial application of NHase.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yunlong Shen, Ruirui Zhang, Xiaohua Jiang, Jinliang Yang
Summary: The study developed a monoclonal antibody, B1M023, that can bind to LILRB1 with high affinity and block its binding to HLA-G. This antibody can promote the activation and IFN-gamma secretion of T cells, suggesting its potential applications in concomitant diagnosis and tumor immunotherapy.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Jun Jiang, Zhengqiang Jiang, Qiaojuan Yan, Susu Han, Shaoqing Yang
Summary: A novel alginate lyase gene from a marine bacterium showed efficient enzyme activity and stability, producing high conversion ratios of alginate oligosaccharides.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xianghua Xiong, Yujin Qiu, Jiahao Zheng, Ling Zhou, Qingyang Wang, Jinglun Pang, Weicai Zhang, Huipeng Chen, Gang Liu, Xiaodong Han
Summary: A specific monoclonal antibody ML419 has been found to disrupt the recognition between Botulinum neurotoxin serotype A (BoNT/A) and FGFR3, effectively preventing BoNT/A from entering neurons. In vivo experiments show that ML419 has a strong protective effect, making it a promising candidate for the development of therapeutics against BoNT/A.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Maria Rain Jennings, Soohyon Min, Grace S. Xu, Kassandra Homayuni, Bhavana Suresh, Yusef Amir Haikal, John Blazeck
Summary: This study successfully produced Homo sapiens adenosine deaminase isoform 1 (HsADA1) and its variants through optimizing the recombinant expression process. The D8N variant of HsADA1 was found to be about 30% less active than the wildtype, but it better retained its activity in human serum. Additionally, the study revealed a previously undescribed phenomenon involving albumin that contributed to the increased activity of HsADA1 and the D8N variant in serum.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Oinam Sangita Devi, Senjam Sunil Singh, K. Rana, Sorokhaibam Jibankumar Singh, Wayenbam Sobhachandra Singh
Summary: A new lectin with hemagglutination activity was purified from the rhizome of Xanthosoma violaceum Schott. The lectin showed different reactions towards human red blood cells of different blood groups. It exhibited optimal hemagglutination activity at specific temperature and pH range, and showed good stability.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
