Article
Chemistry, Physical
Swati Singh, Kaushik Pal
Summary: This research successfully synthesized a polymeric nanosystem that integrates pH-responsive bimetallic oxide-multifunctional nanoparticles and an antitumor drug, which shows promising potential for enhancing tumor treatment. The nanosystem possesses specific targeting, less side effects, and high therapeutic efficacy, making it a multi-purpose nanocarrier for breast cancer therapy.
APPLIED SURFACE SCIENCE
(2023)
Article
Oncology
Ruiyang Zou, Sau Yeen Loke, Veronique Kiak-Mien Tan, Swee Tian Quek, Pooja Jagmohan, Yew Chung Tang, Preetha Madhukumar, Benita Kiat-Tee Tan, Wei Sean Yong, Yirong Sim, Sue Zann Lim, Eunice Png, Shu Yun Sherylyn Lee, Mun Yew Patrick Chan, Teng Swan Juliana Ho, Boon Kheng James Khoo, Su Lin Jill Wong, Choon Hua Thng, Bee Kiang Chong, Yik Ying Teo, Heng-Phon Too, Mikael Hartman, Ngiap Chuan Tan, Ern Yu Tan, Soo Chin Lee, Lihan Zhou, Ann Siew Gek Lee
Summary: Breast cancer screening using mammography often leads to false positive results, prompting unnecessary investigations. This study identified and validated a six-miRNA panel that can differentiate between malignant and benign breast lesions, showing potential for use alongside mammography. Results also revealed an increase in biomarker panel scores with tumor size, stage, and lymph node involvement.
Article
Environmental Sciences
Doyel Roy, Sinchan Adhikari, Ayan Adhikari, Supriya Ghosh, Ikbal Azahar, Debapriya Basuli, Zahed Hossain
Summary: The toxicity of CuO bulk particles was higher than CuO nanoparticles, mainly due to higher Cu ions dissolution.
Article
Chemistry, Analytical
Daohong Wu, Hanwen Lu, Jingrui Wang, Ling Wu, Lin Liu, Xinyao Yi, Jianxiu Wang, Hailin Tang
Summary: Circular RNAs have a closed loop structure and are extensively present in mammalian cells. Evidence suggests their potential association with triple-negative breast cancer. A sandwich structure with ferrocene-capped gold nanoparticle probes was used for sensitive detection of circRNA in clinical samples, demonstrating high potential for clinical applications.
MICROCHEMICAL JOURNAL
(2021)
Article
Multidisciplinary Sciences
Abdulsalam A. Alkhudhayri
Summary: Zinc oxide nanostructures have great potential in various fields such as electronics, catalysis, and solar energy. However, there is limited research on their cytotoxicity against breast cancer cells, especially compared to other nanoparticles. In this study, ZnO-NPs and NRs were synthesized and characterized, and their effects on cancer cell viability and gene expression were evaluated. The results revealed changes in the expression of apoptosis-related genes, indicating the potential of ZnO nanostructures in cancer treatment.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Engineering, Biomedical
Qiang Song, Lu Dou, Wenjin Zhang, Yang Peng, Man Huang, Mengyuan Wang
Summary: Novel and reliable RG combinations for breast cancer research were identified using a public RNA-seq dataset, providing a solid foundation for accurate normalization of qRT-PCR results across different breast cancer tissues and cells.
BIOMEDICAL ENGINEERING ONLINE
(2021)
Article
Oncology
Xuan Zou, Tiansong Xia, Minghui Li, Tongshan Wang, Ping Liu, Xin Zhou, Zebo Huang, Wei Zhu
Summary: A signature of 12 serum miRNAs was identified in this study, showing potential for non-invasive diagnosis of BC.
Article
Oncology
Qinghua Huang, Jianlan Mo, Huawei Yang, Yinan Ji, Rong Huang, Yan Liu, You Pan
Summary: This study identified four different subtypes of breast cancer and constructed a prognostic model involving four m7GRGs, which could predict the prognosis and chemotherapy response of breast cancer. Moreover, candidate drugs related to breast cancer were predicted based on the prognosis model.
Article
Forestry
Jose Luis Ulloa, Paula Aguayo, Daniel Conejera, Rafael Rubilar, Claudio Balocchi, Sofia Valenzuela
Summary: This study identified potential candidate genes involved in drought tolerance in leaves and roots of Eucalyptus globulus through RNA-Seq analysis. Key genes induced by drought stress were discovered, and further validation using qRT-PCR showed differential expression in selected candidate genes in tolerant genotypes. The data generated could provide a basis for future research on drought tolerance mechanisms in E. globulus and for genetic improvement programs.
TREES-STRUCTURE AND FUNCTION
(2022)
Article
Oncology
Minghui Wang, Huilin Liu, Wenyao Wu, Jinxia Zhao, Guanghui Song, Xi Chen, Rong Wang, Changfeng Shao, Jing Li, Haiyan Wang, Qing Wang, Xiaodong Feng
Summary: This study identified three differentially expressed lncRNAs in plasma of breast cancer patients. The results suggest that these three lncRNAs could serve as potential diagnostic biomarkers for breast cancer.
CLINICAL BREAST CANCER
(2022)
Article
Biochemical Research Methods
Hector Apablaza, Myriam Solis, Daniel Conejera, Alexis Fonseca, Jorge Cid, Estefania Tarifeno-Saldivia, Sofia Valenzuela, Veronica Emhart, Marta Fernandez
Summary: The bHLH transcription factor superfamily is widely present in eukaryotes and plays important roles in plant growth, development, and response to abiotic stress. A systematic characterization of the bHLH family in Eucalyptus was conducted for the first time, revealing new gene isoforms produced during cold acclimation in a cold-tolerant hybrid of Eucalyptus. 153 bHLH genes were identified in the Eucalyptus genome, with 44 candidate genes showing alternative splicing events in response to cold stress.
PLANT MOLECULAR BIOLOGY REPORTER
(2022)
Article
Biotechnology & Applied Microbiology
Yue Zhao, Weiwei Xiao, Wanqing Peng, Qinghua Huang, Kunru Wu, Colin E. Evans, Xinguang Liu, Hua Jin
Summary: Encapsulating ORI in biodegradable nanoparticles based on poly-lactic-co-glycolic acid (PLGA) and polyethylene glycol (PEG) significantly enhances its bioactivity and bioavailability against breast cancer, leading to the inhibition of tumor growth and angiogenesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ali Tafti, Samaneh Shojaei, Hakimeh Zali, Saeed Karima, Samira Mohammadi-Yeganeh, Mahdieh Mondanizadeh
Summary: In this study, the researchers investigated the effect of Rapamycin on triple-negative breast cancer (TNBC) cells. They identified important genes and miRNAs targeted by Rapamycin and demonstrated that it suppresses cell migration, induces apoptosis, and has potential therapeutic benefits in TNBC treatment.
MOLECULAR CARCINOGENESIS
(2023)
Article
Biochemistry & Molecular Biology
Nadine de Godoy Torso, Julia Coelho Franca Quintanilha, Maria Aparecida Cursino, Eder de Carvalho Pincinato, Carmen Silvia Passos Lima, Patricia Moriel
Summary: This study aimed to determine the most adequate endogenous normalizer for urinary miRNA relative quantification in head and neck cancer patients treated with cisplatin chemoradiotherapy. RNA-Seq was performed to identify differentially expressed miRNAs between groups with and without cisplatin nephrotoxicity. Four miRNAs were selected as candidate endogenous normalizers and validated by qRT-PCR in a cohort of 49 patients, with hsa-miR-875-5p shown to be an adequate normalizer due to its invariant expression between the two groups.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Shiyu Zhang, Cheng Liu, Xuan Zou, Xiangnan Geng, Xin Zhou, XingChen Fan, Danxia Zhu, Huo Zhang, Wei Zhu
Summary: A three-miRNA panel (miR-146a-5p, miR-24-3p, and miR-93-5p) was successfully established in peripheral serum as a non-invasive biomarker for diagnosing prostate cancer. This panel showed stable diagnostic performance in large cohorts and outperformed other miRNA biomarkers in diagnosing PC.
Article
Environmental Sciences
Murshida Mollik, Md. Habibur Rahman, Majed Al-Shaeri, Ghulam Md. Ashraf, Athanasios Alexiou, Md. Abdul Gafur
Summary: The present study aimed to isolate, characterize, and screen the in vitro antioxidant activity of a pure compound from Black pepper. The results showed that the isolated compound exhibited strong antioxidant activity and had similar characteristics to a standard compound. This research introduced a convenient method for isolating pure compounds from natural sources, which can serve as an important reference for future analysis of crude drugs.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Bilal Ahmed, Asfa Rizvi, Asad Syed, Vishnu D. Rajput, Abdallah M. Elgorban, Salim S. Al-Rejaie, Tatiana Minkina, Mohammad Saghir Khan, Jintae Lee
Summary: The study investigated the accumulation of different forms of aluminum in maize plants and their effects on plant physiology and growth. Results showed that nano-Al2O3 and Al3+ increased aluminum content in maize tissues and induced oxidative stress, leading to negative impacts on seed germination, growth, and photosynthesis. Maize grown hydroponically exhibited higher aluminum toxicity compared to soil-grown maize.
Article
Plant Sciences
Asfa Rizvi, Bilal Ahmed, Mohd Saghir Khan, Vishnu D. Rajput, Shahid Umar, Tatiana Minkina, Jintae Lee
Summary: The indiscriminate discharge and accumulation of heavy metals from anthropogenic sources pose a global threat to food security and human health. The presence of heavy metals in soils and their uptake by maize plants alter the soil-plant systems and pose risks to living organisms. The use of metal tolerant microbiome in enhancing maize yield in contaminated soils is an effective strategy, although limited information is available.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Review
Biochemistry & Molecular Biology
Chenmala Karthika, Agnieszka Najda, Joanna Klepacka, Mehrukh Zehravi, Rokeya Akter, Muhammad Furqan Akhtar, Ammara Saleem, Majed Al-Shaeri, Banani Mondal, Ghulam Md Ashraf, Priti Tagde, Sarker Ramproshad, Zubair Ahmad, Farhat S. Khan, Md Habibur Rahman
Summary: This article focuses on the concept, barriers, and possible treatments for brain tumors, as well as the relationship between brain diseases and the blood-brain barrier, multi-drug resistance, and the use of nanomedicine. The advantages of resveratrol as an antioxidant in treating brain diseases are also explored.
Article
Biochemistry & Molecular Biology
Javed Ahmad, Rizwan Wahab, Maqsood A. Siddiqui, Nida Nayyar Farshori, Quaiser Saquib, Naushad Ahmad, Abdulaziz A. Al-Khedhairy
Summary: Neodymium oxide exhibits cytotoxicity against liver and lung cancer cells at low concentrations, mediated through ROS generation and genotoxicity pathways. The crystallinity and morphology of neodymium oxide were characterized, revealing a curved nanostructure.
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2022)
Article
Chemistry, Inorganic & Nuclear
Rizwan Wahab, Farheen Khan, Manawwer Alam, Javed Ahmad, Abdulaziz A. Al-Khedhairy
Summary: This study designed a sensor material for detecting urea using gamma-Al2O3QDs. The gamma-Al2O3QDs were processed and characterized, and their sensing efficiency with urea solution was tested. The performance of the gamma-Al2O3QDs/GCE sensor was evaluated with different parameters and a real sample analysis was conducted.
INORGANIC CHEMISTRY COMMUNICATIONS
(2023)
Article
Toxicology
Rizwan Wahab, Farheen Khan, Neha Kaushik, Nagendra Kumar Kaushik, Linh Nhat Nguyen, Eun Ha Choi, Maqsood A. Siddiqui, Nida Nayyar Farshori, Quaiser Saquib, Javed Ahmad, Abdulaziz A. Al-Khedhairy
Summary: Nano-based Core-shell ZnO microspheres have shown potential effectiveness in treating liver cancer by regulating cell proliferation rate and inducing apoptosis against cancer cells. This treatment exhibits cytotoxic response in human cultured cells and regulates the expression of apoptotic and anti-apoptotic genes. The application of nanotechnology in cancer treatment is of great significance.
TOXICOLOGY IN VITRO
(2022)
Review
Microbiology
Sahar Zaidi, Khursheed Ali, Asad U. U. Khan
Summary: The concept of microorganisms has often been viewed from a pathogenic perspective, but it is now being reconsidered for its importance to human health. Microorganisms play a major role in shaping the human immune system and predisposing individuals to diseases. The human body is inhabited by a diverse range of bacteria, known as the microbiota, which makes up 0.3% of body mass. This review focuses on the inheritance of microbiota, the composition of microbiome in different body sites, dysbiosis-induced pathologies, factors affecting microbiome composition, strategies of the immune system to prevent dysbiosis, dysbiosis-induced biofilms, and the significance of microbiome in medical therapeutics. The article aims to provide a comprehensive understanding of human microbiota for the urgent need of protocol standardization.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mohammad Rafe Hatshan, Quaiser Saquib, Maqsood A. A. Siddiqui, Mohammad Faisal, Javed Ahmad, Abdulaziz A. Al-Khedhairy, Mohammed Rafi Shaik, Mujeeb Khan, Rizwan Wahab, Valeria De Matteis, Syed Farooq Adil
Summary: Recent studies have investigated the potential applications of surface-tailored graphene oxide (GO) as an anticancer agent. However, the efficacy of nonfunctionalized graphene oxide nanolayers (GRO-NLs) as an anticancer agent has not been well explored. In this study, GRO-NLs were synthesized and their in vitro anticancer potential was evaluated in breast, colon, and cervical cancer cells. The results showed that GRO-NLs exhibited cytotoxicity and induced apoptosis in these cancer cells through various mechanisms, including mitochondrial dysfunction and upregulation of genes involved in apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Hatem A. A. Abuelizz, Ahmed H. H. Bakheit, Mohamed Marzouk, Waled M. M. El-Senousy, Mohamed M. M. Abdellatif, Gamal A. E. Mostafa, Quaiser Saquib, Sawsan B. B. Hassan, Rashad Al-Salahi
Summary: This study investigated the anti-coxsackievirus B4 activity of the target benzo[g]quinazolines in BGM cells, and compounds 1-3 showed the highest activity. Molecular docking revealed that compounds 1-3 interacted with the constitutive amino acids in the active site of coxsackievirus B4. Further experimental research is needed to determine the exact mechanism of action of benzo[g]quinazolines.
PHARMACOLOGICAL REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Sahar Zaidi, Khursheed Ali, Yadya M. Chawla, Asad U. Khan
Summary: Bacterial cells are surrounded by a peptidoglycan (PG) cell wall, which is essential for cell integrity and biogenesis pathways. The mltG gene plays a crucial role in the synthesis of peripheral PG and in bacterial virulence. Knocking out the mltG gene in Streptococcus mutans significantly compromised the bacterial growth and virulence potential through dysregulation of important genes involved in biofilm, metabolism, and stress response.
Article
Pharmacology & Pharmacy
Inji Park, Afreen Jailani, Jin-Hyung Lee, Bilal Ahmed, Jintae Lee
Summary: Biofilms are responsible for persistent or recurring microbial infections. Polymicrobial biofilms are prevalent in environmental and medical niches. Dual-species biofilms formed by UPEC and S. aureus are commonly found in urinary tract infection sites. ATO nanoparticles showed strong antimicrobial and antibiofilm properties against single- and dual-species biofilms formed by UPEC and S. aureus, making them potential candidates for controlling these infections.
Article
Virology
Ahmed H. Bakheit, Quaiser Saquib, Sarfaraz Ahmed, Sabiha M. Ansari, Abdullah M. Al-Salem, Abdulaziz A. Al-Khedhairy
Summary: COVID-19, caused by SARS-CoV-2, has caused significant loss of life. In the search for solutions, the researchers evaluated the inhibitory effects of ten phytochemicals isolated from Saudi medicinal plants on the RNA-dependent RNA polymerase (RdRp) of the virus. Compounds 7, 8, and 9 demonstrated non-nucleoside and irreversible interaction capabilities with RdRp, suggesting their potential as antivirals against SARS-CoV-2.
Review
Toxicology
Saeed Alqahtani, Shaherah Alqahtani, Quaiser Saquib, Fayaz Mohiddin
Summary: Plastic waste has significantly impacted the environment, accumulating in water and land ecosystems and harming all forms of life. During the COVID-19 pandemic, the production of plastic had reached unprecedented levels, which generate microplastics (MPs) and nanoplastics (NPs) when burned. MPs and NPs have been detected in various locations, but our understanding of their potential adverse health effects is limited. This review aims to provide a comprehensive overview of the sources, interactions, ecotoxicity, routes of exposure, toxicity mechanisms, detection methods, and future directions for the safety evaluation of MPs and NPs.
FRONTIERS IN TOXICOLOGY
(2023)
Article
Materials Science, Biomaterials
Khursheed Ali, Sahar Zaidi, Aijaz A. Khan, Asad U. Khan
Summary: In this study, ET-NPs were synthesized at different pH values and characterized using advanced techniques. The surface activities of ET-NPs, T-NPs, and T-MPs in planktonic bacteria, biofilm, GM bacterial communities, and animal organs were assessed using flow cytometry, TEM, EDS, histopathology, RT-PCR, 16S-rRNA metagenomics, and ELISA. Electron-microscopic, NMR, FTIR, DLS, XRD, and EDS analyses confirmed the EGCG coronation, dispersity, size-stability of ET-NPs, and crystallinity and elemental composition of ET-NPs-8 and T-NPs. In addition, FCM, RT-PCR, 16S-rRNA metagenomics, histopathology, SEM, and EDS analyses demonstrated that EGCG coronation in ET-NPs-8 enhanced the penetration into body organs and metabolically active bacterial communities of GM.
BIOMATERIALS ADVANCES
(2023)
