期刊
ACTA PHARMACOLOGICA SINICA
卷 36, 期 4, 页码 528-534出版社
ACTA PHARMACOLOGICA SINICA
DOI: 10.1038/aps.2015.3
关键词
IL-37b; breast cancer; anti-cancer effect; mitomycin C; T cell; tumor microenvironment
资金
- National Basic Research Program of China [2013CB966904, 2015CB964402]
- National Natural Science Foundation of China [81421002, 81273217, 81322007, 81170007]
- Tianjin Research Program of Application Foundation and Advanced Technology [12JCYBJC32800]
- Technology Foundation for Selected Overseas Chinese Scholars
- Fok Ying-Tong Education Foundation of China [131039]
- Recruitment Program of Global Youth Experts
Aim: IL-37b has shown anti-cancer activities in addition to its anti-inflammatory properties. In this study, we investigated the effects of IL-37b on breast carcinoma growth in mice and to determine the involvement of T cell activation in the effects. Methods: IL-37b gene was transferred into mouse breast carcinoma cell line 4T1 (4T1-IL37b cells), the expression of secretory IL-37b by the cells was detected, and the effects of IL-37b expression on the cell proliferation in vitro was evaluated. After injection of 4T1 cells or 4T1-IL37b cells into immunocompetent BALB/c mice, immunodeficient BALB/c nude mice and NOD-SCID mice, the tumor growth and survival rate were measured. The proliferation of T cells in vitro was also detected. Results: IL-37b was detected in the supernatants of 4T1-IL37b cells with a concentration of 12.02+/-0.875 ng/mL. IL-37b expression did not affect 4T1 cell proliferation in vitro. BALB/c mice inoculated with 4T1-IL37b cells showed significant retardation of tumor growth. BALB/c mice inoculated with both 4T1 cells and mitomycin C-treated 4T1-IL37b cells also showed significant retardation of tumor growth. But the anti-cancer activity of IL-37b was abrogated in BALB/c nude mice and NOD-SCID mice inoculated with 4T1-IL37b cells. Recombinant IL-37b slightly promoted CD4(+) T cell proliferation without affecting CD8(+) T cell proliferation. Conclusion: IL-37b exerts anti-4T1 breast carcinoma effects in vivo by modulating the tumor microenvironment and influencing T cell activation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据