Article
Cell Biology
Maria Norte-Munoz, Fernando Lucas-Ruiz, Alejandro Gallego-Ortega, David Garcia-Bernal, Francisco J. Valiente-Soriano, Pedro de la Villa, Manuel Vidal-Sanz, Marta Agudo-Barriuso
Summary: The study examines the impact of different types of transplants on neuroprotection and axonal regeneration, finding that syngrafts significantly influence the survival and regeneration of neurons and axons, while xenografts may lead to retinal issues and allografts have limited effects.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Keane Jared Guillaume Kenswil, Paola Pisterzi, Gonzalo Sanchez-Duffhues, Claire van Dijk, Andrea Lolli, Callie Knuth, Byambasuren Vanchin, Adrian Christopher Jaramillo, Remco Michiel Hoogenboezem, Mathijs Arnoud Sanders, Jacqueline Feyen, Tom Cupedo, Ivan G. Costa, Ronghui Li, Eric Monique Johannes Bindels, Kirsten Lodder, Bianca Blom, Pieter Koen Bos, Marie-Jose Goumans, Peter ten Dijke, Eric Farrell, Guido Krenning, Marc Hermanus Gerardus Petrus Raaijmakers
Summary: Rare LNGFR(+) cells in human fetal and regenerative bone marrow co-express endothelial and stromal markers, display features of endothelial-to-mesenchymal transition, and can generate tissue-forming BMSCs. EndoMT shows robust and sustained contributions to bone precursor and hematopoietic niche pools, with IL-33 overexpression driving the conversion process.
Article
Cell & Tissue Engineering
Charlotte Marx, Sophia Gardner, Rebecca M. Harman, Bettina Wagner, Gerlinde R. Van de Walle
Summary: The study found that secreted factors from equine MSCs significantly decreased the viability of methicillin-resistant Staphylococcus aureus bacteria in mature biofilms in a novel skin biofilm explant model, and also demonstrated that equine MSCs secrete CCL2, which increases the antimicrobial activity of equine keratinocytes. These findings contribute to understanding the antimicrobial properties of the MSC secretome, supporting the value of MSC secretome-based treatments for infected wounds.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Cell & Tissue Engineering
Behnoosh Tayebi, Mahnaz Babaahmadi, Mohammad Pakzad, Mostafa Hajinasrollah, Farhad Mostafaei, Shahrbanoo Jahangiri, Amir Kamali, Hossein Baharvand, Mohamadreza Baghaban Eslaminejad, Seyedeh-Nafiseh Hassani, Ensiyeh Hajizadeh-Saffar
Summary: Mesenchymal stromal cells (MSCs) have shown potential in treating inflammatory and non-inflammatory diseases, but their clinical applications require strict evaluations and monitoring to ensure compliance with safety standards. This study aimed to assess the potential toxicity effects of cryopreserved human bone marrow-derived clonal MSCs in animal models during different time periods.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Immunology
Fulvio Massaro, Florent Corrillon, Basile Stamatopoulos, Nathan Dubois, Achille Ruer, Nathalie Meuleman, Dominique Bron, Laurence Lagneaux
Summary: Mesenchymal stromal cells (MSC) play a critical role in aging and inflammaging, and studying them is important for understanding the mechanisms of diseases in the elderly.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Yujie Song, Christian Jorgensen
Summary: This review highlights the potential of mesenchymal stromal cells (MSCs) in treating osteoarthritis (OA), focusing on their chondrogenic and immunomodulatory potential. The review provides an overview of the effects of MSCs in preclinical and clinical studies of OA treatment, comparing different MSC sources. Furthermore, the review summarizes the current evidence for the assessment of cartilage in OA using magnetic resonance imaging (MRI) and second-look arthroscopy after MSC therapy.
Article
Health Care Sciences & Services
Suncica Kapor, Milica Vukotic, Tijana Suboticki, Dragoslava Dikic, Olivera Mitrovic Ajtic, Milica Radojkovic, Vladan P. Cokic, Juan F. Santibanez
Summary: The study found that HU inhibits the growth of BMMSC, causing senescence-like changes and affecting cellular differentiation and immunoregulatory functions.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Article
Biochemistry & Molecular Biology
Chiara Valsecchi, Stefania Croce, Alice Maltese, Lorenza Montagna, Elisa Lenta, Alice Nevone, Maria Girelli, Paolo Milani, Tiziana Bosoni, Margherita Massa, Carlotta Abba, Rita Campanelli, Jessica Ripepi, Annalisa De Silvestri, Adriana Carolei, Giovanni Palladini, Marco Zecca, Mario Nuvolone, Maria Antonietta Avanzini
Summary: Immunoglobulin light-chain amyloidosis (AL) is characterized by misfolded light chains, and this study aimed to characterize bone marrow (BM)-MSCs from AL patients. The results showed that AL-MSCs were comparable to those from healthy donors in morphology and differentiation capacities, but had a reduced proliferation rate compared to healthy donor MSCs. Additionally, the modulatory effect of AL-MSCs on plasma cells was similar to that of healthy donor MSCs.
Article
Cell & Tissue Engineering
Maiko Sezaki, Subinoy Biswas, Sayuri Nakata, Motohiko Oshima, Shuhei Koide, Nicole Pui Yu Ho, Nobukazu Okamoto, Takeshi Miyamoto, Atsushi Iwama, Hitoshi Takizawa
Summary: This study characterizes the heterogeneity of hMSCs and their ability to efficiently form humanized ossicles in good donors by correlating with the frequency and functionality of chondrocyte progenitors. CD271(+)CD51(+) stromal cell subset was found to possess enhanced hMSC activity, with PALLADIN-negative cells displaying the greatest CFU-F potential in subsets enriched for greater chondrogenic ability. The findings contribute to better understanding and isolation of ossicle-forming hMSCs for optimized engineering of human bone organs.
STEM CELLS AND DEVELOPMENT
(2021)
Review
Cell Biology
Kevin Woods, Borhane Guezguez
Summary: Mesenchymal stromal cells (MSCs) are a diverse cell population known for their nutrient-producing and immunomodulatory functions, crucial in regulating hematopoietic stem cells (HSC) in the bone marrow for blood production and immunity. MSCs contribute to bone integrity and hormone supply by differentiating into osteoblasts and adipocytes, playing a key role in the complex interactions of the HSC niche.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Kevin Woods, Borhane Guezguez
Summary: MSCs are a heterogeneous cell population found in various tissues in the body, with important regulatory functions, especially in the bone marrow where they play a critical role in regulating HSCs. They contribute to maintaining bone integrity and hormonal nutrition supply, as well as regulating the homeostasis and differentiation of the HSC niche through differentiation into different cell types. Research on MSCs helps understand their role in regulating cell interactions and homeostasis in the body.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Oncology
Patricia Maiso, Pedro Mogollon, Enrique M. Ocio, Mercedes Garayoa
Summary: Multiple myeloma is a cancer of plasma cells that accumulate in the bone marrow, and the interaction between mesenchymal stromal cells and myeloma cells appears to play a crucial role in the progression of the disease. Structural and functional differences between mesenchymal stromal cells from healthy donors and myeloma patients have been identified, suggesting a significant contribution to the pathophysiology of multiple myeloma.
Article
Biochemistry & Molecular Biology
Fatih Karadeniz, Jung Hwan Oh, Mi Soon Jang, Youngwan Seo, Chang-Suk Kong
Summary: Bone marrow adiposity is a complication in osteoporotic patients, and phytochemicals can alleviate it. This study evaluated the effect of libanoridin on the adipogenic differentiation of bone marrow-derived mesenchymal stromal cells. The results showed that libanoridin inhibited adipogenic differentiation through suppressing MAPK-mediated PPAR gamma signaling. Future studies will further explore the anti-adipogenic effects of libanoridin in vivo and elucidate its action mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Zsolt Matula, Gabor Mikala, Szilvia Lukacsi, Janos Matko, Tamas Kovacs, Eva Monostori, Ferenc Uher, Istvan Valyi-Nagy
Summary: Mitochondrial transfer plays a crucial role in drug resistance acquisition in multiple myeloma, with bi-directional transfer occurring through tunneling nanotubes and partial cell fusion, especially under the influence of chemotherapeutic drugs. By influencing tumor metabolism and using oxidative phosphorylation inhibitors alongside chemotherapy, the supportive effect of stromal cells can be effectively avoided in myeloma cells.
Article
Emergency Medicine
Kazuyuki Miyamoto, Shino Katsuki, Hiroki Yamaga, Motoyasu Nakamura, Keisuke Suzuki, Gen Inoue, Masaharu Yagi, Jun Sasaki, Kenji Dohi, Munetaka Hayashi
Summary: Guillain-Barre syndrome (GBS) is a progressive disease characterized by symmetric muscle weakness. Early diagnosis and treatment are crucial for improving patient outcomes. Detailed medical history collection and considering GBS as a differential diagnosis in emergency settings where neurological examination is not feasible are important steps in the diagnostic process.
AMERICAN JOURNAL OF EMERGENCY MEDICINE
(2022)
Article
Multidisciplinary Sciences
Kazuyuki Miyamoto, Motoyasu Nakamura, Hirokazu Ohtaki, Keisuke Suzuki, Hiroki Yamaga, Kaoru Yanagisawa, Atsuo Maeda, Masaharu Yagi, Munetaka Hayashi, Kazuho Honda, Kenji Dohi
Summary: Global warming increases the incidence of heatstroke, which can cause neurological symptoms and damage to the cerebellum. In a mouse heatstroke model, motor coordination disorder was observed 3 weeks after heatstroke and gradually improved to some extent. Although white matter demyelination was detected in the cerebellum 1 and 3 weeks after heatstroke, it was not found in the corpus callosum. The number of Purkinje cells significantly decreased at 1, 3, and 9 weeks after heatstroke. The intensity of synaptophysin and postsynaptic density-95 temporarily attenuated at 3 weeks but intensified at 9 weeks after heatstroke. Motor coordination loss occurred a few weeks after heatstroke and recovered partially. Late-onset motor impairment may be caused by cerebellar dysfunctions, as assessed by morphological changes in cerebellar white matter and Purkinje cells. The number of Purkinje cells did not recover for 9 weeks, but other factors, including motor coordination, partially recovered, possibly through synaptic reconstruction and cerebellar white matter remyelination. These findings are associated with late-onset neurological deficits and recovery after heatstroke.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Keisuke Suzuki, Kazuyuki Miyamoto, Shutaro Ozawa, Raichi Kawakami, Motoyasu Nakamura, Gen Inoue, Akihito Kato, Yoko Tarumi, Masaharu Yagi, Kenji Dohi
Correction
Multidisciplinary Sciences
Keisuke Suzuki, Kazuyuki Miyamoto, Shutaro Ozawa, Raichi Kawakami, Motoyasu Nakamura, Gen Inoue, Akihito Kato, Yoko Tarumi, Masaharu Yagi, Kenji Dohi
Article
Medicine, General & Internal
Kazuyuki Miyamoto, Yuki Kaki, Shino Katsuki, Atsuo Maeda, Motoyasu Nakamura, Keisuke Suzuki, Masaharu Yagi, Jun Sasaki, Kenji Dohi, Munetaka Hayashi
Summary: This article presents a clinical case of non-ST segment elevation myocardial infarction in an 82-year-old female patient. The patient initially presented with epigastric and back pain, but showed no abnormalities on electrocardiography and serum biomarkers, eventually leading to the diagnosis of subendocardial infarction through CT imaging.
AMERICAN JOURNAL OF CASE REPORTS
(2022)
Article
Neurosciences
Akira Yoshikawa, Hirokazu Ohtaki, Kazuyuki Miyamoto, SungHyek Kim, Kazunori Hase, Makoto Yoshida, Shotaro Kamijo, Sawa Kamimura, Nobuyoshi Koiwa, Masahiko Izumizaki
Summary: This study used three-dimensional kinematic analysis to evaluate motor dysfunction and recovery in mice with brain damage after mild-intensity running exercise. The results showed that appropriate exercise can recover motor function, and three-dimensional kinematic analysis is a powerful tool for detecting minor behavioral alterations on the affected side and compensatory movements on the unaffected side.
Article
Multidisciplinary Sciences
Kaede Hiruma, Keisuke Suzuki, Akihito Kato, Hiroki Yamaga, Motoyasu Nakamura, Gen Inoue, Yuki Kaki, Kazuyuki Miyamoto, Masaharu Yagi, Kenji Dohi
Summary: A 23-year-old man presented with extensive subcutaneous emphysema after a minor injury, with no active suspicion of necrotizing fasciitis based on tests. He was observed for one day and discharged. The mechanism is unclear.
Editorial Material
Critical Care Medicine
Kenko Aoki, Ayako Watanabe, Yosuke Kakisaka, Motoyasu Nakamura, Yasuo Fujita, Kenji Dohi
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
(2023)
Article
Biochemistry & Molecular Biology
Keisuke Suzuki, Hiroki Yamaga, Hirokazu Ohtaki, Satoshi Hirako, Kazuyuki Miyamoto, Motoyasu Nakamura, Kaoru Yanagisawa, Takuya Shimada, Tomohiko Hosono, Hitoshi Hashimoto, Kazuho Honda, Kenji Dohi
Summary: Heat stroke is a life-threatening illness caused by high ambient temperatures and humidity. This study found that PACAP knockout mice showed greater resistance to heat exposure compared to wild-type mice, possibly due to lower activity of temperature-sensitive neurons and differences in heat production mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Motoki Fujita, Yasutaka Oda, Kotaro Kaneda, Tadashi Kaneko, Eiichi Suehiro, Kenji Dohi, Yasuhiro Kuroda, Hitoshi Kobata, Ryosuke Tsuruta, Tsuyoshi Maekawa
Summary: This study aimed to investigate the association between the temperature difference between the jugular bulb and pulmonary artery (Delta Tjb-pa) and the neurological outcome of patients with severe traumatic brain injury (TBI). The results showed that a reduction in Delta Tjb-pa and greater variation in Delta Tjb-pa were associated with an unfavorable outcome, especially in patients treated with mild therapeutic hypothermia (TH).
Article
Multidisciplinary Sciences
Yuki Setoguchi, Akiko Hayashi, Ayami Kawada, Ayako Ibusuki, Daigo Yanaoka, Ryota Saito, Tomoko Ishibashi, Hiroaki Takimoto, Yoshihide Yamaguchi, Hirokazu Ohtaki, Hiroko Baba
Summary: Intravenous immunoglobulin (IVIg) has been effective in treating inflammatory demyelinating diseases by modulating peripheral nervous system (PNS) demyelination. In this study, IVIg was found to bind to a 36-kDa membrane glycoprotein, identified as large myelin protein zero (L-MPZ), in the sciatic nerve. IVIg penetrated the demyelinating lesion and deposited on L-MPZ in the myelin debris, leading to a decreased demyelination area. These findings suggest that IVIg may act by binding to L-MPZ on myelin debris.
PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Gen Inoue, Yuhei Ohtaki, Kazue Satoh, Yuki Odanaka, Akihito Katoh, Keisuke Suzuki, Yoshitake Tomita, Manabu Eiraku, Kazuki Kikuchi, Kouhei Harano, Masaharu Yagi, Naoki Uchida, Kenji Dohi
Summary: Increased oxidative stress is important in the pathogenesis of critically ill patients requiring intensive care. Sedatives, commonly used for sedation in these patients, have antioxidant properties. This study aimed to evaluate the direct scavenging activity of sedative agents on various free radicals.
Article
Multidisciplinary Sciences
Keisuke Suzuki, Kazuyuki Miyamoto, Takahiro Kanai, Mariko Kurihara, Kazuki Kikuchi, Kohei Harano, Akihito Kato, Masaharu Yagi, Yoshimitsu Ohgiya, Kenji Dohi
Summary: Heat stroke can lead to multiple organ dysfunction and death. Some patients may develop neurological sequelae, such as cerebellar ataxia, during the recovery phase. However, there is currently no reliable method to predict the neurological prognosis, and the usefulness of imaging remains inconclusive.