期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 243, 期 -, 页码 127-134出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2015.09.010
关键词
Trillin; Acute lung injury; Nrf2; HO-1; NF-kappa B
资金
- National Twelve Five Major Drug Discovery Project [2011ZX09102-002-01]
- Natural Science Foundation of China [81302839]
Inflammation response and oxidative stress have been reported to be involved in the pathogenesis of acute lung injury (ALI). Accordingly, anti-inflammatory treatment is proposed to be a possible efficient therapeutic strategy for ALI. The purpose of our present study was to evaluate the anti-inflammatory efficacy of trillin (Tr) on ALI induced by lipopolysaccharide (LPS) in mice and explore the underlying mechanism. BALB/c mice received Tr (50, 100 mg/kg) intraperitoneally 1 h prior to the intratracheal instillation of lipopolysaccharide (LPS) challenge. Pretreatment with Tr at the dose of 50, 100 mg/kg markedly ameliorated lung wet-to-dry weight (W/D) ratio, myeloperoxidase (MPO) activity and pulmonary histopathological conditions. In addition, the protective efficacy of Tr might be attributed to the down-regulations of neutrophil infiltration, malondialdehyde (MDA), inflammatory cytokines and the up-regulations of super-oxide dismutase (SOD), catalase(CAT), glutathione(GSH), Glutathione Peroxidase(GSH-Px) in bronchoalveolar lavage fluid (BALF). Meanwhile, our study revealed some correlations between (NF-E2-related factor 2) Nrf2/heme oxygenase (HO)-1/nuclear factor-kappa B (NF-kappa B) pathways and the beneficial effect of Tr, as evidenced by the significant up-regulations of HO-1 and Nrf2 protein expressions as well as the down-regulations of p-NF-kappa B and p-inhibitor of NF-kappa B (I kappa B) in lung tissues. Taken together, our results indicated that Tr exhibited protective effect on LPS-induced ALI by the regulations of related inflammatory events via the activations of Nrf2, HO-1 and NF-kappa B pathway. The current study indicated that Tr could be a potentially effective candidate medicine for the treatment of ALI. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
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