期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 248, 期 -, 页码 1-7出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2016.01.020
关键词
Caffeine; Anti-inflammatory; LPS; NF-kappa B; MAPKs
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2013R1A1A2064024]
- National Research Foundation of Korea [2013R1A1A2064024] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Caffeine is a white crystalline xanthine alkaloid found in the seeds of coffee plants and leaves of the tea bush. In this study, we evaluated whether caffeine exerts anti-inflammatory effects on lipopolysaccharide (LPS)-induced inflammation both in vitro and in vivo. RAW264.7 cells were treated with various concentrations of caffeine in the presence or absence of LPS. Caffeine decreased the LPS-induced inflammatory mediator, nitric oxide (NO). Caffeine treatment also reduced the expression of pro-inflammatory genes, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin (IL)-3, IL-6 and IL-12, and decreased both IL-6 secretion and phosphorylated p38MAPK expression in LPS-treated RAW264.7 cells. Caffeine inhibited nuclear translocation of nuclear factor kappa B (NF-kappa B) via I kappa B alpha phosphorylation. In addition, caffeine inhibited LPS-induced NO production in zebrafish. These results suggest that caffeine may suppress LPS-induced inflammatory responses in RAW264.7 cells by regulating NF-kappa B activation and MAPK phosphorylation. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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