期刊
NUCLEIC ACIDS RESEARCH
卷 48, 期 10, 页码 5383-5396出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkaa289
关键词
-
资金
- SingaporeMinistry of Education Academic Research Fund (AcRF) Tier 3 [MOE2012-T3-1-001]
- SingaporeMinistry of Education Academic Research Fund (AcRF) Tier 2 [MOE2018-T2-1-112]
- NTU Institute of Structural Biology (NISB)
- Nanyang Technological University funds
Telomeres protect the ends of our chromosomes and are key to maintaining genomic integrity during cell division and differentiation. However, our knowledge of telomeric chromatin and nucleosome structure at the molecular level is limited. Here, we aimed to define the structure, dynamics as well as properties in solution of the human telomeric nucleosome. We first determined the 2.2 degrees A crystal structure of a human telomeric nucleosome core particle (NCP) containing 145 bp DNA, which revealed the same helical path for the DNA as well as symmetric stretching in both halves of the NCP as that of the 145 bp '601' NCP. In solution, the telomeric nucleosome exhibited a less stable and a markedly more dynamic structure compared to NCPs containing DNA positioning sequences. These observations provide molecular insights into how telomeric DNA forms nucleosomes and chromatin and advance our understanding of the unique biological role of telomeres.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据