期刊
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
卷 32, 期 6, 页码 889-894出版社
HIGHER EDUCATION PRESS
DOI: 10.1007/s40242-016-6251-y
关键词
Magnetic; Fe3O4@SiO2; Protein A; Nanoparticle; Monoclonal antibody
资金
- National Natural Science Foundation of China [51572058, 91216123, 51174063]
- Natural Science Foundation of Heilongjiang Province, China [E201436]
- International Science & Technology Cooperation Program of China [2013DFR10630, 2015DFE52770]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [SRFDP 20132302110031]
Magnetic Fe3O4@SiO2 nanoparticles with superparamagnetic properties were prepared via a reverse microemulsion method at room temperature. The as-prepared samples were characterized by transmission electron microscopy( TEM), X-ray diffractometry(XRD), and vibrating sample magnetometry(VSM). The Fe3O4@SiO2 nanoparticles were modified by (3-aminopropyl) triethoxysilane(APTES) and subsequently activated by glutaraldehyde(Glu). Protein A was successfully immobilized covalently onto the Glu activated Fe3O4@SiO2 nanoparticles. The adsorption capacity of the nanoparticles was determined on an ultraviolet spectrophotometer(UV) and approximately up to 203 mg/g of protein A could be uniformly immobilized onto the modified Fe3O4@SiO2 magnetic beads. The core-shell of the Fe3O4@SiO2 magnetic beads decorated with protein A showed a good binding capacity for the chimeric anti-EGFR monoclonal antibody(anti-EGFR mAb). The purity of the anti-EGFR mAb was analyzed by virtue of HPLC. The protein A immobilized affinity beads provided a purity of about 95.4%.
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