期刊
NEURO-ONCOLOGY
卷 22, 期 10, 页码 1463-1473出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/neuonc/noaa075
关键词
CX3CR1; glioma; malignant transformation; polymorphism; tumor microenvironment
资金
- National Institutes of Health [R01NS094615, R25NS070694]
- Tumor SPORE [P50CA127001]
- Marnie Rose Foundation
- NCI [CA016672]
Background. Chemokine signaling may contribute to progression of low-grade gliomas (LGGs) by altering tumor behavior or impacting the tumor microenvironment. In this study, we investigated the role of CX3C chemokine receptor 1 (CX3CR1) signaling in malignant transformation of LGGs. Methods. Ninety patients with LGGs were genotyped for the presence of common CX3CR1 V2491 polymorphism and examined for genotype-dependent alterations in survival, gene expression, and tumor microenvironment. A genetically engineered mouse model was leveraged to model endogenous intracranial gliomas with targeted expression of CX3C ligand 1 (CX3CL1) and CX3CR1, individually or in combination. Results. LGG patients who were heterozygous (V/I; n = 43) or homozygous (I/I; n = 2) for the CX3CR1 V2491 polymorphism had significantly improved median overall (14.8 vs 9.8 y, P< 0.05) and progression-free survival (8.6 vs 6.5 y, P< 0.05) compared with those with the wild type genotype (V/V; n= 45). Tumors from the V/I + Ill group exhibited significantly decreased levels of CCL2 and MMP9 transcripts, correlating with reduced intratumoral M2 macrophage infiltration and microvessel density. In an immunocompetent mouse model of LGGs, coexpression of CX3CL1 and CX3CR1 promoted a more malignant tumor phenotype characterized by increased microglia/macrophage infiltration and microvessel density, resulting in shorter survival. Conclusions. CX3CR1V2491 polymorphism is associated with improved overall and progression-free survival in LGGs. CX3CR1 signaling enhances accumulation of tumor associated microglia/macrophages and angiogenesis during malignant transformation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据