Article
Plant Sciences
Kuen-Jin Tsai, Der-Fen Suen, Ming-Che Shih
Summary: We studied the role of Arabidopsis HRM1 in hypoxic stress response. HRM1 was found to be regulated by EIN3 and RAP2.2 and enriched in mitochondria where it associates with complex-I and attenuates mitochondrial electron transport chain activity. Loss of HRM1 led to increased mitochondrial respiration rates under hypoxia and higher tolerance to submergence.
Editorial Material
Biochemistry & Molecular Biology
William B. McKean, Ashish G. Toshniwal, Jared Rutter
Summary: Luengo et al. (2020) demonstrate that overactivation of pyruvate dehydrogenase (PDH) impairs NAD(+) regeneration by altering mitochondrial membrane potential and driving ATP synthesis beyond demand, leading some cells to prioritize aerobic glycolysis to meet the requirement for oxidized cofactors in biosynthetic metabolism.
Article
Cell Biology
Xuan Wang, Yuting Ji, Jingyi Qi, Shuaishuai Zhou, Sitong Wan, Chang Fan, Zhenglong Gu, Peng An, Yongting Luo, Junjie Luo
Summary: Cervical cancer is a deadly disease in women, and mitochondrial-mediated ferroptosis (MMF) has been identified as a key factor in its development. By investigating the mitochondria transmembrane candidates, researchers discovered that mitochondrial carrier 1 (MTCH1) plays a central role in MMF in cervical cancers. MTCH1 deficiency disrupted mitochondrial oxidative phosphorylation and increased mitochondrial reactive oxygen species (ROS), leading to ferroptosis. Targeting MTCH1 in combination with Sorafenib effectively inhibited cervical cancer growth by inducing ferroptosis in a mouse xenograft model. These findings provide insights into the mechanisms of MMF and offer a potential therapeutic target for cervical cancer.
CELL DEATH & DISEASE
(2023)
Article
Microbiology
Rachel Leonard, Yuan Tian, Feng Tan, Giel van Dooren, Jenni Hayward
Summary: TgApiCox13 is a critical component of the electron transport chain in the parasite Toxoplasma gondii, and is homologous to human protein HsMiNT. Both proteins bind two Fe-S clusters, which play a crucial role in the function of the electron transport chain.
Review
Biochemistry & Molecular Biology
Ami Kobayashi, Toshihiko Takeiwa, Kazuhiro Ikeda, Satoshi Inoue
Summary: The mitochondrial electron transport chain (ETC) is crucial for energy production and drives various biochemical processes in eukaryotic cells through oxidative phosphorylation (OXPHOS). Recent studies have shown that noncoding RNAs (ncRNAs), including microRNAs (miRNAs), transfer-RNA-derived fragments (tRFs), long ncRNAs (lncRNAs), and circular RNAs (circRNAs), play important roles in regulating ETC and OXPHOS systems. This review highlights the emerging roles of ncRNAs in the regulation of mitochondrial ETC and OXPHOS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Shanshan Liu, Song Fu, Guodong Wang, Yu Cao, Lanlan Li, Xuemei Li, Jun Yang, Ning Li, Yabing Shan, Yang Cao, Yan Ma, Mengqiu Dong, Qinghua Liu, Hui Jiang
Summary: Through experimental validation, Gro3P synthesis has been identified as an endogenous pathway for NAD(+) regeneration, effectively restoring the effects of inhibited electron transport chain on cells and maintaining a certain consistency in different organisms. This discovery has the potential to become a therapeutic target for mitochondrial complex I diseases.
Article
Multidisciplinary Sciences
Tanya J. Espino-Sanchez, Henry Wienkers, Rebecca G. Marvin, Shai-Anne Nalder, Aldo E. Garcia-Guerrero, Peter E. VanNatta, Yasaman Jami-Alahmadi, Amanda Mixon Blackwell, Frank G. Whitby, James A. Wohlschlegel, Matthew T. Kieber-Emmons, Christopher P. Hill, Paul A. Sigala
Summary: The functions of Plasmodium malaria parasites' cytochrome c remain unclear. They express two distinct cytochrome c homologs, c and c-2, with little sequence similarity. Knockdown or knockout of cytochrome c-2 had little impact on parasite growth, indicating the reliance on the more conserved cytochrome c for mitochondrial function. Biochemical and structural studies showed that cytochrome c-2 has an unusually open active site and can bind exogenous small molecules. These findings provide important insights into the cytochrome functions in malaria parasites.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Applied
Ling Li, Hiroaki Kitazawa, Xinhua Zhang, Liming Zhang, Yang Sun, Xiangyou Wang, Zhanli Liu, Yanyin Guo, Shaoxuan Yu
Summary: The study found that white mushrooms treated with 0.1mM melatonin exhibited good quality, reduced electron leakage, maintained higher ATP levels, prevented cytochrome c release, and inhibited senescence by enhancing mitochondrial function.
Article
Cell Biology
Xiphias Ge Zhu, Aleksey Chudnovskiy, Lou Baudrier, Benjamin Prizer, Yuyang Liu, Benjamin N. Ostendorf, Norihiro Yamaguchi, Abolfozl Arab, Bernardo Tavora, Rebecca Timson, Soren Heissel, Elisa de Stanchina, Henrik Molina, Gabriel D. Victora, Hani Goodarzi, Kivanc Birsoy
Summary: This study reveals that loss of heme synthesis reduces tumor growth in PDAC, and autophagy plays a crucial role in immune evasion of pancreatic tumors.
Article
Biochemistry & Molecular Biology
Viktoriia Bazylianska, Akhil Sharma, Heli Chauhan, Bernard Schneider, Anna Moszczynska
Summary: Methamphetamine is a highly abused stimulant that is neurotoxic and increases the risk of Parkinson's disease. The study found that Methamphetamine itself contributes to mitochondrial dysfunction in the striatum, while dopamine only decreases the levels of certain subunits. The study also suggests that parkin does not regulate the turnover of NDUFS3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Zhanqiang Du, Xiaoting Zhou, Yuezheng Lai, Jinxu Xu, Yuying Zhang, Shan Zhou, Ziyan Feng, Long Yu, Yanting Tang, Weiwei Wang, Lu Yu, Changlin Tian, Ting Ran, Hongming Chen, Luke W. Guddat, Fengjiang Liu, Yan Gao, Zihe Rao, Hongri Gong
Summary: Human complex II is a protein complex that plays a crucial role in connecting the tricarboxylic acid cycle and oxidative phosphorylation. Mutations in this complex have been found to cause mitochondrial disease and certain types of cancers. By using cryoelectron microscopy, the structure of human complex II has been determined, revealing its subunits and providing insights into its functional aspects. Mapping clinically relevant mutations onto the structure helps explain their disease-causing potential.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Pharmacology & Pharmacy
Joseph A. Combs, Chandler H. Monk, Mark A. A. Harrison, Elizabeth B. Norton, Cindy A. Morris, Deborah E. Sullivan, Kevin J. Zwezdaryk
Summary: Studies suggest that drugs targeting host cell mitochondria can inhibit HCMV replication. Using rotenone, oligomycin, antimycin, and metformin can reduce HCMV titers, indicating the dependency of HCMV replication on functional mitochondria.
ANTIVIRAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Mahfuzur Rahman, Maximilian Billmann, Michael Costanzo, Michael Aregger, Amy H. Y. Tong, Katherine Chan, Henry N. Ward, Kevin R. Brown, Brenda J. Andrews, Charles Boone, Jason Moffat, Chad L. Myers
Summary: FLEX is a pipeline that establishes reference standards for benchmarking human genome-wide CRISPR screen data, identifying a predominant mitochondria-associated signal within co-essentiality networks. By characterizing functional bias associated with mitochondria genes, it provides insights into interpreting differential hits in CRISPR screening. The utility of the FLEX pipeline for performing robust comparative evaluations of CRISPR screens or methods for processing them is demonstrated.
MOLECULAR SYSTEMS BIOLOGY
(2021)
Article
Cell Biology
Hannah Neiswender, Frederick C. Baker, Rajalakshmi Veeranan-Karmegam, Phylicia Allen, Graydon B. Gonsalvez
Summary: We identified the Drosophila ortholog of TTC1 (dTtc1) as an interacting partner of Egalitarian, an RNA adaptor of the Dynein motor. Depletion of dTtc1 resulted in defective oogenesis and swollen mitochondria in egg chambers. dTtc1 interacts with electron transport chain (ETC) components and is required for stabilizing ETC components.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Environmental Sciences
Garam An, Taeyeon Hong, Hahyun Park, Whasun Lim, Gwonhwa Song
Summary: In this study, the developmental toxicity of the carbamate insecticide oxamyl on zebrafish larvae was examined. Oxamyl was found to have adverse effects on the development of the neuron, notochord, and vascular system. Furthermore, it was found to impact the mitochondrial electron transport chain and the PI3K/Akt and p38 Mapk signaling pathways in zebrafish.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
