期刊
MICROCHEMICAL JOURNAL
卷 154, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.microc.2020.104598
关键词
Deep eutectic solvents; Mulberry leaves; Phenolic compounds; Microwave-assisted extraction; Kinetics; Macroporous resin
资金
- Fundamental Research Funds for the Central Universities [2572018AA17, 2572017EA03, 2572017ET03, 2572017DA04, 2572015EA04, 2572016AA48]
- National Key R&D Program of China [2016YFD0600805]
- Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team)
Green and tailor-made deep eutectic solvents (DESs) coupled with microwave-assisted extraction (MAE) were performed to extract phenolic compounds from mulberry leaves. Among the twelve DESs prepared, Choline chloride/Glycerol (1:2) with 20% water content was more efficient to get high extraction yields of phenolics. Then, several essential parameters of microwave-assisted deep eutectic solvents extraction (DES-MAE) were optimized by single-factor and Box-Behnken design (BBD) experiments. According to the result of optimization, the total extraction yield of target phenolic compounds reached 8.352 mg/g at microwave temperature 66 degrees C for extracting 18 min with the liquid/solid ratio of 20 mL/g, which was obviously superior to conventional solvent extraction. In addition, the extraction kinetic models confirmed that microwave-assisted extraction was a more efficient extraction technique than ultrasound-assisted extraction and heat-refluxing extraction. Meanwhile, the macroporous resin was utilized to enrich and separate target compounds from DES extracts, and the recovered DES still exhibited a good solvent effect to be applied for re-extraction. Therefore, DES-MAE followed by macroporous resin column chromatography was a green and integrated strategy for the extraction and preparative separation of active ingredients from plant materials.
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