Article
Public, Environmental & Occupational Health
Vanesa Garcia, Luz Leston, Ana Parga, Isidro Garcia-Menino, Javier Fernandez, Ana Otero, John E. Olsen, Ana Herrero-Fresno, Azucena Mora
Summary: This study investigated 31 strains of Escherichia coli from different sources using genomic comparison, bladder-cell, and biofilm formation assays. It found that animal-derived and human extraintestinal infection strains had high genomic similarity and exhibited similar pathogenic characteristics in cell and biofilm assays. These results highlight the potential role of food-producing animals as a source of urinary tract infections.
Article
Public, Environmental & Occupational Health
Ali Qasemi, Fateh Rahimi, Mohammad Katouli
Summary: The prevalence of biofilm and beta-lactamase-producing UPEC strains differed significantly between Isfahan and Zahedan in Iran, with higher rates observed in the city of Zahedan. This suggests that socioeconomic status and environmental factors may play a role in the pathogenicity of these strains.
PATHOGENS AND GLOBAL HEALTH
(2022)
Article
Biotechnology & Applied Microbiology
Ali Qasemi, Fateh Rahimi, Mohammad Katouli
Summary: The study investigated the genetic diversity and virulence-associated genes of biofilm-producing UPEC strains isolated from patients with UTI in Iran. Strong BFP strains showed higher levels of VAGs, while weak BFP strains exhibited more pronounced presence of specific VAGs. There exists a high genetic diversity among the BFP strains with distinct profiles of virulence-associated genes.
INTERNATIONAL MICROBIOLOGY
(2022)
Article
Microbiology
A. E. Shea, A. E. Frick-Cheng, S. N. Smith, H. L. T. Mobley
Summary: This study demonstrates that phenotypic characteristics can be used to predict the severity of urinary tract infection caused by uropathogenic Escherichia coli (UPEC) strains. These characteristics can help differentiate UPEC from other E. coli strains and reduce the overuse of antibiotics.
Article
Biochemistry & Molecular Biology
Monika Karczewska, Patryk Strzelecki, Krystyna Bogucka, Katarzyna Potrykus, Agnieszka Szalewska-Palasz, Dariusz Nowicki
Summary: Urinary tract infections are common bacterial diseases worldwide, with UPECs being the most prominent group among the pathogens responsible. In this study, we examined 118 UPEC isolates to determine their genetic background, antibiotic resistance, and their ability to form biofilms and induce stress responses. Our findings showed that these UPEC strains expressed unique attributes and had a significant ability to form biofilms and accumulate multi-resistance traits, which were crucial for the development of severe infections in a Galleria mellonella model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Rongrong Wu, Ashok Kumar Kumawat, Isak Demirel
Summary: Urinary tract infections (UTIs) caused by uropathogenic E. coli (UPEC) are common in humans. Trimethylamine N-oxide (TMAO), a proinflammatory metabolite, has been linked to vascular inflammation and chronic kidney disease. This study investigated the effects of TMAO on UTIs and found that it aggravated the release of inflammatory mediators and enhanced UPEC colonization of bladder epithelial cells. These findings suggest a potential role of TMAO in infectious diseases, providing a basis for further research on the link between diet, gut microbiota, and UTIs.
Article
Microbiology
Ulises Hernandez-Chinas, Maria E. Chavez-Berrocal, Ricardo E. Ahumada-Cota, Armando Navarro-Ocana, Luz M. Rocha-Ramirez, Yolanda Perez-del Mazo, Maribel Alvarado-Cabello, Gabriel Perez-Soto, Luis A. Leon-Alamilla, Salvador E. Acevedo-Monroy, Diego Esquiliano, Atlantida M. Raya-Rivera, Carlos A. Eslava
Summary: The study suggests that autologous bacterial lysates (ABL) may have a therapeutic effect on children with recurrent urinary tract infections (rUTI) by controlling the infection for almost one year.
Article
Cell Biology
Ashok Kumar Kumawat, Geena Varghese Paramel, Kartheyaene Jayaprakash Demirel, Isak Demirel
Summary: The study showed that renal fibroblasts, but not renal epithelial cells, release IL-1 beta during a UPEC infection, which is dependent on factors like NLRP3, caspase-1, and caspase-4. Additionally, the UPEC virulence factor alpha-hemolysin was found to be necessary for IL-1 beta release.
Article
Microbiology
Bharath Reddy Boya, Jin-Hyung Lee, Jintae Lee
Summary: The three chloroindoles investigated in this study demonstrated antibiofilm, antimicrobial, and antivirulence properties against Uropathogenic Escherichia coli (UPEC) and other nosocomial pathogens. They inhibited biofilm formation, motilities, and virulence gene expressions, suggesting their potential as therapeutic agents to combat urinary tract infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Paulo A. Schueroff, Fabia A. Salvador, Cecilia M. Abe, Haleluya T. Wami, Eneas Carvalho, Rodrigo T. Hernandes, Ulrich Dobrindt, Tania A. T. Gomes, Waldir P. Elias
Summary: The study revealed a hybrid strain UPEC-46 with characteristics of both EAEC and UPEC, with a significant role of AFP adhesin in adherence to colorectal and bladder cell lines.
Article
Biotechnology & Applied Microbiology
Hiral Patel, Devarshi Gajjar
Summary: This study quantified the biofilm formation in UTI isolates of Pseudomonas aeruginosa and found that strong biofilm producers exhibited increased cell adhesion and twitching motility. The biofilms formed by strong producers were thicker compared to weak ones.
Article
Medicine, General & Internal
Katarina Mestrovic Popovic, Petra Povalej Brzan, Tomaz Langerholc, Natasa Marcun Varda
Summary: Probiotics may be helpful in alleviating UTI symptoms and preventing UTI recurrence.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Medicine, Research & Experimental
Manuel G. Ballesteros-Monrreal, Pablo Mendez-Pfeiffer, Edwin Barrios-Villa, Margarita M. P. Arenas-Hernandez, Yessica Enciso-Martinez, Cesar O. Sepulveda-Moreno, Enrique Bolado-Martinez, Dora Valencia
Summary: This study systematically reviewed published reports on the virulence genes, antibiotic resistance, and phylogenetic groups of uropathogenic Escherichia coli (UPEC) in the Mexican population. The findings revealed that multidrug-resistant UPEC strains are highly prevalent in Mexico, reducing the therapeutic options available. This has significant implications for the diagnosis and treatment of urinary tract infections.
ARCHIVES OF MEDICAL RESEARCH
(2023)
Article
Microbiology
Ivana Kerkez, Paul M. Tulkens, Tanel Tenson, Francoise Van Bambeke, Marta Putrins
Summary: Research shows that commonly used antibiotics for urinary tract infections are much less effective against intracellular UPEC compared to extracellular UPEC. Except fluoroquinolones, most antibiotics were unable to achieve a bactericidal effect intracellularly at clinically achievable concentrations. Fluoroquinolones ciprofloxacin and finafloxacin killed almost all extracellular bacteria at concentrations close to the MIC, but significantly higher concentrations were needed for intracellular bacteria.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Hannah Q. Hughes, Kyle A. Floyd, Sajjad Hossain, Sweta Anantharaman, David T. Kysela, Mikls Zoldi, Laszl Barna, Yuanchen Yu, Michael P. Kappler, Triana N. Dalia, Ram C. Podicheti, Douglas B. Rusch, Meng Zhuang, Cassandra L. Fraser, Yves Brun, Stephen C. Jacobson, James B. McKinlay, Fitnat H. Yildiz, Elizabeth M. Boon, Ankur B. Dalia
Summary: This research uncovers a regulatory pathway in Vibrio cholerae that allows rapid termination of biofilm formation, dependent on the phosphodiesterase CdpA and the signaling molecule nitric oxide. This sheds light on how bacterial species integrate environmental cues to regulate pilus dynamics and attachment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemical Research Methods
Shruthi Shankar Raman, Vedha Hari B. Narayanan, Ramyadevi Durai
Summary: This study aimed to improve the solubility and dissolution kinetics of Lamotrigine by combining Lamotrigine nanoparticles with polymer composite oral dissolving film. The optimized polymeric nanoparticles exhibited monodispersed particles with high entrapment efficiency, while the composite oral films showed significantly higher drug release compared to pure drug. The nanoparticles displayed mild drug-polymer interactions, enhanced solubility and dissolution of the drug, and lower cytotoxicity to normal cells, suggesting their potential for in vivo administration.
ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES
(2021)
Article
Chemistry, Inorganic & Nuclear
Jayasankari Senthilganesh, Sivaranjani Ravichandran, Rubini Durairajan, Siva BalaSubramaniyan, Lakshmi Krishnasamy, Anbazhagan Veerappan, Nithyanand Paramasivam
Summary: Vulvovaginal candidiasis is a common infection caused by Candida species, especially Candida albicans. Plant protein-based nano scaffolds were found to effectively mitigate fluconazole-resistant Candida albicans infections. Lectin-functionalized copper sulfide nanoparticles showed lower inhibitory concentrations and synergistic effects with antifungal drugs like micafungin.
JOURNAL OF CLUSTER SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Murthy Vignesh, Shankar Ramakrishanan Madhan Shankar, Davoodbasha MubarakAli, Bodethala Narayanan Vedha Hari
Summary: The novel strain of Bacillus NKMV-3 isolated from rhizosphere is an effective biocontrol agent against Alternaria solani, the pathogen causing early blight disease in tomato. The strain contains antibiotic synthesis genes and lipopeptides, confirmed through gene amplification and liquid chromatography. Formulations based on this strain may offer an effective solution for controlling early blight disease in tomato and other crops.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sowndarya Jothipandiyan, Devarajan Suresh, Sankaran Venkatachalam Sankaran, Subbiah Thamotharan, Kumaravel Shanmugasundaram, Preethi Vincent, Saravanan Sekaran, Shanmugaraj Gowrishankar, Shunmugiah Karutha Pandian, Nithyanand Paramasivam
Summary: The study confirmed the novel thiazolinyl-picolinamide based palladium(II) complexes as effective in disrupting biofilms and inhibiting quorum sensing in Acinetobacter baumannii. The best Pd(II) complex showed potent inhibitory activity against virulence factors and strains of the pathogen, indicating promising anti-infective potential in combating implant infections.
Article
Pharmacology & Pharmacy
Jayasankari Senthilganesh, Lakshmi Deepak, Ramyadevi Durai, Vedha Hari B. Narayanan, Anbazhagan Veerappan, Nithyanand Paramasivam
Summary: Vulvovaginal Candidiasis (VVC) is a global issue for women of the reproductive age group. This study proposes a novel formulation that undergoes sol-to-gel transition and combines with standard antifungal drugs for VVC treatment. The formulated in-situ gel shows biofilm eradication against C. albicans and C. glabrata, and the researchers also explore the use of goat buccal mucosal membrane as an ex-vivo model for studying vaginal biofilms. The in vitro and ex vivo drug release kinetics demonstrate the effectiveness of the drug-loaded JCuSNPs formulation.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Priya K. Dharshini, Ramya D. Devi, S. Banudevi, Vedha Hari B. Narayanan
Summary: By using spray drying technology, chitosan nanoparticles were successfully developed to enhance the solubility and bioavailability of dolutegravir (DTG). The nanoparticles showed a significant increase in C-max and improved bioavailability compared to pure DTG. This study demonstrates the potential of chitosan-based nanoparticles as carriers for oral administration of DTG in pediatric HIV patients.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Bozena Hosnedlova, Daniil Kabanov, Marta Kepinska, Vedha Hari B. Narayanan, Arli Aditya Parikesit, Carlos Fernandez, Geir Bjorklund, Hoai Viet Nguyen, Awais Farid, Jiri Sochor, Agnes Pholosi, Mojmir Baron, Milan Jakubek, Rene Kizek
Summary: Silver nanoparticles (AgNPs) synthesized using plant extracts, especially L. indica, exhibit strong antibacterial and antibiofilm effects.
Article
Biotechnology & Applied Microbiology
Murthy Vignesh, ShankarRamakrishanan Madhan Shankar, Nagaraj Subramani, Bodethala Narayanan VedhaHari, Durai Ramyadevi
Summary: This research explores the efficacy of Bacillus velezensis NKMV-3 strain and its lipopeptide extract against early blight caused by Alternaria solani. Spray-dried tablets of this strain and extract were found to be stable and showed inhibitory effects against A.solani, making them suitable candidates for bio control of tomato early blight.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Vedha Hari B. Narayanan, Artur Lewandowski, Ramyadevi Durai, Weronika Gonciarz, Pawel Wawrzyniak, Marek Brzezinski
Summary: This study developed a long-acting injectable formulation for the prolonged release of Tenofovir alafenamide-chitosan polymeric nanoparticles using ethyl cellulose oleogels, which showed potential for the treatment of patients infected with HIV/AIDS.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Menon Archana, Durairajan Rubini, Krishnan Priya Dharshini, Bodethala Narayanan Vedha Hari, Senthilganesh Jayasankari, Durai Ramyadevi, Weronika Gonciarz, Agnieszka Domanska, Marek Brzezinski, Paramasivam Nithyanand
Summary: This study focuses on 3D printing a urinary catheter with anti-infective properties by incorporating the antibiotic secnidazole. The optimized catheter has dimensions of 5mm outer diameter, 3.5mm inner diameter, and 50mm length. The drug-loaded catheter showed a sustained release of secnidazole, effectively disrupting biofilms and suppressing virulence factors of urinary tract infection-causing pathogens.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Gopalakrishnan Thamilselvan, Helma David, Anusree Sajeevan, Shobana Rajaramon, Adline Princy Solomon, Ramya Devi Durai, Vedha Hari B. Narayanan
Summary: This study explores the use of a smart hydrogel delivery system for the treatment of S. aureus-induced soft skin infections. The system combines the antibiotic Ciprofloxacin (CIP) and an efflux pump inhibitor 5-Nitro-2-(3-phenylpropoxy) pyridine (5-NPPP) to re-sensitize the pathogen towards antibiotics and reduce antimicrobial resistance. The study involves the fabrication of CIP-loaded polyvinyl alcohol (PVA) hydrogels and the development of polymeric nanoparticles for the controlled release of 5-NPPP. In vivo studies using Balb/c mice models demonstrate the effectiveness of the dual drugs loaded hydrogels in reducing bacterial load and promoting re-epithelization.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
K. Malarvizhi, B. N. Vedha Hari, P. Rajalakshmi, S. Devaraj, Ramyadevi Durai
Summary: This study analyzes the preparation and properties of metallopharmaceuticals, providing scientific evidence for their stability and efficacy. It helps overcome the quality and safety concerns of metal-mineral constituents in the formulation.
RSC MEDICINAL CHEMISTRY
(2023)
Proceedings Paper
Materials Science, Multidisciplinary
B. N. Vedha Hari, N. Sathiya Narayanan, N. Baskar, N. Sriraman, V. S. P. Suraj Nanduru
Summary: This study integrates surface texturing and extended self-lubrication to achieve a hassle-free and toxic-free working environment for machining grey cast iron materials. The experimental results show that using semi-solid lubricant filled textured tools can reduce cutting forces and friction coefficients, particularly at higher cutting speeds. However, it was found that the texture surfaces and lubricant-filled gaps away from the cutting edge did not contribute to the cutting performance.
MATERIALS TODAY-PROCEEDINGS
(2022)
Review
Pharmacology & Pharmacy
Archana Menon, Rubini Durairajan, Ramyadevi Durai, Nithyanand Paramasivam, VedhaHari B. Narayanana
Summary: Three-dimensional printing is a pioneering technology that can be used to manufacture catheters with antimicrobial properties and alter their physical properties to ease discomfort. New technologies and materials are needed to overcome existing challenges and limitations.
CRITICAL REVIEWS IN THERAPEUTIC DRUG CARRIER SYSTEMS
(2022)
