Article
Engineering, Environmental
Jing Ji, Tianpeng Gao, El-Sayed Salama, Marwa M. El-Dalatony, Liang Peng, Yuxing Gong, Pu Liu, Xiangkai Li
Summary: This study developed a novel whole-cell biocatalyst to accelerate the degradation of antibiotics in pharmaceutical wastewater. By displaying β-lactamase on the cell surface, the biocatalyst showed high enzyme activity and stable performance, significantly improving antibiotic removal and overall reactor performance.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
O. S. Savinova, A. V. Shabaev, O. A. Glazunova, K. V. Moiseenko, T. V. Fedorova
Summary: Basidiomycetes have gained a lot of attention for their exceptional ability to degrade phthalic acid esters (PAEs). This article focuses on the degradation process of benzyl butyl phthalate (BBP) and diisobutyl phthalate (DiBP) by the fungus Trametes hirsuta. The study reveals that T. hirsuta can destroy more than 95% of the initial BBP within 24 hours, and complete destruction occurs by the 3rd day of cultivation. The degradation of DiBP is slower, with 95% of the initial DiBP being destroyed within 10 days. Enzymes involved in the degradation process were identified, and unique metabolites were found through GC-MS analysis. The toxicity assessment shows a pronounced toxic effect of the metabolites formed during the degradation of BBP.
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Mengyao Yao, Jingran Qian, Xiaoni Chen, Jilong Liu, Xiaoqing Yang, Peng Gao, Cheng Zhang
Summary: Agricultural films are widely used in high-intensity agriculture, but the low recovery rate leads to an accumulation of plastic mulch in agricultural soils. This study focuses on the exposure impact of BBP, a chemical introduced into soil ecosystems through the accumulation of plastic mulch, on earthworms. The study explores the effects of BBP on the digestive system, oxidative stress, and neurotoxicity in earthworms, providing insights into the molecular mechanisms underlying these effects.
Article
Environmental Sciences
Huan Wang, Congyu Li, Guokai Yan, Yanjie Zhang, Haiyan Wang, Weiyang Dong, Zhaosheng Chu, Yang Chang, Yu Ling
Summary: This study investigates the spatiotemporal distributions of six phthalic acid esters (PAEs) in the surface sediments of the Songhua River. The results show that the concentration of PAEs is higher in the spring compared to summer and autumn, and the upper reaches of the river have higher PAEs concentrations than the middle and lower reaches. Dibutyl phthalate (DBP) and di(2-ethylhexyl) phthalate (DEHP) are the most abundant PAEs. Ecological risk assessment reveals that DBP and DEHP pose moderate or high risks to aquatic organisms. Agricultural sources are identified as the primary contributor to the PAEs pollution.
ENVIRONMENTAL POLLUTION
(2023)
Article
Agronomy
Jianpeng Gao, Qinghong Sun, Yuan-Liang Liu, Wei-Jie Xiong, Si-Han Zeng, Yulong Zhang, Yongtao Li, Hui-Juan Xu
Summary: We investigated the toxicological effects of single and combined exposure of the plasticizer di-(2-ethylhexyl) phthalate (DEHP) and the fluoroquinolone antibiotic enrofloxacin (ENR) on earthworms (Eisenia fetida) by analyzing oxidative stress, DNA damage, and changes in digestibility. Both DEHP and combined pollution treatments resulted in increased reactive oxygen species content in earthworms, while ENR alone had little effect. Malonaldehyde (MDA) was mainly cleared by glutathione sulfur transferase (GST), and DEHP caused more DNA damage than combined pollution. Both single and combined pollution reduced digestive enzyme activity, with combined pollution having a stronger effect. Cellulase, MDA, and GST were the most sensitive indicators. The toxicity ranking based on the Integrated Biological Response (IBR) index was ENR + DEHP > DEHP > ENR, showing a synergistic effect of combined toxicity. These findings highlight the significant ecological risk of phthalate esters and antibiotics in the environment.
Article
Environmental Sciences
Hanbo Chen, Peng Qin, Xing Yang, Amit Bhatnagar, Sabry M. Shaheen, Jorg Rinklebe, Fengchang Wu, Song Xu, Lei Che, Hailong Wang
Summary: This study investigated the impact of plant- and animal-derived biochar on the competitive sorption-desorption of potentially toxic elements (PTEs) and phthalic acid esters (PAEs) in soils with different organic carbon content. The sorption behavior was influenced by biochar type, application dose, and soil organic carbon content. Additionally, the reciprocal effect between DEP and Cd2+ was found to be a crucial factor affecting their sorption/desorption behavior by biochar, with pig carcass biochar showing greater efficacy in metal/DEP immobilization.
ENVIRONMENTAL RESEARCH
(2021)
Article
Food Science & Technology
Salvatore Velotto, Jonathan Squillante, Agata Nolasco, Raffaele Romano, Teresa Cirillo, Francesco Esposito
Summary: This study evaluated the concentration of DEHP and DBP in coffee powder and beverages, and assessed their migration from different packaging and machines. The results showed higher levels of DEHP in coffee beverages extracted by a professional machine than in coffee powder. However, the levels of PAEs did not exceed the specific migration limits, and exposure to PAEs from coffee beverages was low, indicating that coffee can be considered a safe beverage in terms of PAE exposure.
Article
Engineering, Environmental
Xiru Chen, Xinyue Gu, Chao Wang, Liuqing Huang, Dingding Wu, Hao Wu, Sijia Liang, Jingyi Ling, Cheng Gu
Summary: Phthalic acid esters (PAEs), commonly used as plasticizers to improve the flexibility of materials, are widely distributed in soil and wastewater, raising public concerns due to their endocrine-disrupting effects. A ferrihydrite-H2O2-bisulfite ternary system was developed for efficient degradation of dimethyl phthalate (DMP) in this study. Hydroxyl radical (・OH) was identified as the dominant reactive species for DMP degradation. The study also showed that bisulfite-assisted heterogeneous Fenton-like reactions could be a potential in-situ soil remediation technology for efficient degradation of PAEs.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Jun Ma, Yingang Lu, Ying Teng, Changyin Tan, Wenjie Ren, Xueying Cao
Summary: This study analyzed the residue characteristics and pollution sources of six priority PAEs in tobacco and soils, with DEHP and DBP being the most abundant. The main sources of PAEs in the tobacco-soil system were identified as plastic films, fertilizers, and pesticides.
Article
Marine & Freshwater Biology
Hui Chen, Weiwei Feng, Kun Chen, Xuchun Qiu, Hai Xu, Guanghua Mao, Ting Zhao, Xiangyang Wu, Liuqing Yang
Summary: This study assessed the effects of DBP and DiBP on early development of zebrafish offspring, showing that parental exposure to these chemicals can lead to increased hatchability and prevalence of malformations in F1 larvae. Transcriptomic analysis revealed different molecular mechanisms of parental co-exposure compared to exposure to individual chemicals.
AQUATIC TOXICOLOGY
(2021)
Article
Environmental Sciences
Junmei Ding, Yan Liu, Yanxiu Gao, Chengbo Zhang, Yafei Wang, Bo Xu, Yunjuan Yang, Qian Wu, Zunxi Huang
Summary: This study isolated a bacterium capable of degrading lambda-cyhalothrin and developed a whole cell biocatalyst for this process. The research demonstrates that the whole cell biocatalyst shows better stability and degradation efficiency under lower temperature conditions.
Article
Environmental Sciences
Cheng Zhang, Xiaoqing Yang, Zhangjia Wang, Yinglin Liu, Mengyao Yao, Lusheng Zhu, Peng Gao, Zhenyu Wang
Summary: This study assessed the co-exposure effects of butyl benzyl phthalate (BBP) and titanium dioxide nanomaterials (nTiO(2)) on Metaphire guillelmi, a soil organism. The results showed that BBP and nTiO(2) had detrimental effects on the gut and intestinal bacteria of Metaphire guillelmi. This study provides evidence for the sustainable application and scientific management of these compounds.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Abu Baker, Bilal Ahmad, Khaloud Mohammed Alarjani, Noura Saleem Aldosri, Mohd Sajid Khan
Summary: The marine bacterium Rhodovulum sp. DBP07 was found to efficiently degrade DBP, with glucose identified as a key nutrient factor influencing both growth and degradation rate. Additionally, the choice of carbon sources also impacts the degradation of DBP. Statistical optimization results showed enhanced biodegradation rate under specific conditions.
Article
Biotechnology & Applied Microbiology
Yan Liu, Xiaoliang Wang, Sujin Nong, Zehui Bai, Nanyu Han, Qian Wu, Zunxi Huang, Junmei Ding
Summary: In this study, a whole cell biocatalyst was constructed by displaying carbaryl hydrolase on the surface of Escherichia coli cells for bioremediation, showing better stability and maintained catalytic activity compared to purified enzyme. This surface-displayed strategy provides a new solution for cost-efficient bioremediation of carbaryl and potentially other carbamates in environmental bioremediation.
MICROBIAL CELL FACTORIES
(2022)
Article
Chemistry, Physical
Yuting Sun, Ming Guo, Shengnan Hu, Xiaobo Fang, Zhicheng Jin, Ronghui Wu
Summary: In this study, a novel immobilized enzyme (MIP-HNTs@lipase) was successfully prepared by combining molecular imprinting and immobilized enzyme technology for the efficient degradation of PAEs. The MIP-HNTs@lipase showed good stability and high degradation efficiency towards DEHP. This research provides a new strategy for the industrial application of enzymes and the degradation of persistent pollutants.
MOLECULAR CATALYSIS
(2022)
Article
Biochemical Research Methods
Junmei Ding, Hujie Zhu, Yujia Ye, Jie Li, Nanyu Han, Qian Wu, Zunxi Huang, Zhaohui Meng
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2018)
Article
Biotechnology & Applied Microbiology
Junmei Ding, Yang Zhou, Hujie Zhu, Ming Deng, Liangchuan Long, Yunjuan Yang, Qian Wu, Zunxi Huang
BIOTECHNOLOGY LETTERS
(2019)
Article
Environmental Sciences
Junmei Ding, Yan Liu, Yanxiu Gao, Chengbo Zhang, Yafei Wang, Bo Xu, Yunjuan Yang, Qian Wu, Zunxi Huang
Summary: This study isolated a bacterium capable of degrading lambda-cyhalothrin and developed a whole cell biocatalyst for this process. The research demonstrates that the whole cell biocatalyst shows better stability and degradation efficiency under lower temperature conditions.
Article
Engineering, Environmental
Yan Liu, Shukun Tang, Xu Wang, Xiaoliang Wang, Xianghua Tang, Qian Wu, Zunxi Huang, Junmei Ding
Summary: The long-term and excessive use of pyrethroid pesticides poses substantial health risks and ecosystem concerns. A novel carboxylesterase, EstGS1, was characterized in this study, showing the ability to hydrolyze pyrethroid pesticides. EstGS1 exhibited optimal activity at 60 degrees C and pH 8.5 using pNPC2 as substrate. It is a halotolerant esterase and remains stable in high salt concentrations. This study presents the first report on a pyrethroid pesticide hydrolase characterized from a halophilic actinobacteria.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Xiaoliang Wang, Yibo Li, Yan Liu, Qian Wu, Zunxi Huang, Shukun Tang, Junmei Ding
Summary: The newly discovered acetyl xylan esterase ThAXE from Thermobifida halotolerans has high catalytic activity towards p-nitrophenyl acetate and can hydrolyze the acetyl group of 7-ACA. The catalytic residues of ThAXE were identified through molecular docking and site-directed mutagenesis. This novel acetyl xylan esterase has potential biotechnological values.
PROCESS BIOCHEMISTRY
(2023)
