Article
Biochemistry & Molecular Biology
Eiseul Kim, Seung-Min Yang, Dae-Hyun Jung, Hae-Yeong Kim
Summary: In this study, a novel approach combining mass spectrometry and machine learning techniques was used to accurately identify the important food-fermenting bacteria Weissella cibaria and W. confusa. Through protein analysis and statistical analysis, it was found that although these two bacterial species exhibited similar protein profiles, they could be differentiated and classified using machine learning methods. The combination of mass spectrometry and machine learning enables accurate microbial identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Ting Cai, Qiu-Huan Zhao, Wen-Liang Xiang, Lin Zhu, Yu Rao, Jie Tang
Summary: The HigBA system of Weissella cibaria 018 responded to bile salt stress but not acid stress, providing new insights into the tolerance mechanism of probiotics in gastrointestinal environments.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Food Science & Technology
Neha Sharma, Deepshikha Gupta, Young-Seo Park
Summary: This study compared the genomes of W. cibaria YRK005 and W. confusa CCK931 isolated from young radish and kimchi, respectively. The genomic sizes of W. cibaria YRK005 and W. confusa CCK931 were 2.36 Mb (45%) and 2.28 Mb (44.67%), respectively. The study identified 92 and 83 CAZymes genes for W. cibaria YRK005 and W. confusa CCK931, respectively, including glycoside hydrolases and glycosyl transferases. Both strains have genes for carbohydrate esterases and carbohydrate-binding modules. The primary CAZymes involved in oligosaccharide utilization were GH1, GH2, GH30, GH13_30, GH13_31, GH42, GH43, and GH65. The study also described the production pathways for glycogen and folate in both strains.
FOOD SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
Mustafa Tahsin Yilmaz, Humeyra Ispirli, Osman Taylan, Mohammed Alamoudi, Enes Dertli
Summary: A slimy-mucinous-type colony of EPS-producing Weissella cibaria PDER21 was isolated and identified. The EPS was found to be a glucan type homopolysaccharide composed of glucose monomers. It exhibited superior thermal stability and antioxidant activity, as well as good water binding capacity and solubility. The findings suggest that the glucan PDER21 has great potential for use in the food industry.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Applied
Fan Zhang, Lin Wang, Zihao Zhang, Baodong Zheng, Yi Zhang, Lei Pan
Summary: The study investigates the structure and cryoprotective activity of the exopolysaccharide EPS821-1 produced by Weissella cibaria. EPS821-1 is found to have a novel glucan structure and exhibits excellent cryoprotective activities for Bifidobacterium longum subsp. longum F2. This research provides scientific evidence and insights for the potential application of EPS821-1 as a cryoprotectant in the food industry.
Article
Multidisciplinary Sciences
Pavlo Khodakivskyi, Christian L. Lauber, Aleksey Yevtodiyenko, Arkadiy A. Bazhin, Stephen Bruce, Tamar Ringel-Kulka, Yehuda Ringel, Bertrand Betrisey, Joana Torres, Jianzhong Hu, Chieh Jason Chou, Elena A. Goun
Summary: The study developed a novel optical tool for quantifying BSH activity across various biological contexts in a quick and cost-effective manner, with potential clinical applications, particularly in predicting the clinical status of IBD patients.
Article
Food Science & Technology
Soyoung Park, Kandasamy Saravanakumar, Anbazhagan Sathiyaseelan, SeonJu Park, Xiaowen Hu, Myeong-Hyeon Wang
Summary: The present study isolated and identified five lactic acid bacteria from Korean kimchi, with Weissella cibaria producing a high level of exopolysaccharide (EPS) compared to other isolates. The EPS from W. cibaria was found to be composed of glucose and galactose, linked through specific monosaccharides. It exhibited strong antioxidant activity and biocompatibility, making it a promising candidate for biomedical applications.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Avinash Kant Lakra, Mahesh Ramatchandirane, Sandeep Kumar, Kitlangki Suchiang, Venkatesan Arul
Summary: The probiotic bacteria Weissella cibaria MD2 was used to produce exopolysaccharide (EPS) with excellent antioxidant activities, which can increase oxidative stress tolerance and enhance the lifespan of C. elegans.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Lauren E. E. Lynch, Amy B. B. Hair, Krishnakant G. G. Soni, Heeju Yang, Laura A. A. Gollins, Monica Narvaez-Rivas, Kenneth D. R. Setchell, Geoffrey A. A. Preidis
Summary: Cholestasis refers to impaired bile flow from the liver to the intestine. In neonates, cholestasis causes poor growth and may progress to liver failure and death. This study examined the development of the liver-gut-microbiome axis and the effects of cholestasis on this development in extremely preterm infants. The results showed that cholestasis interrupts the development of the secondary bile acid biosynthesis pathway and bile salt hydrolase genes, which are important for normal bile flow.
Article
Food Science & Technology
Jimmy G. Hernandez-Gomez, Argelia Lopez-Bonilla, Gabriela Trejo-Tapia, Sandra V. Avila-Reyes, Antonio R. Jimenez-Aparicio, Humberto Hernandez-Sanchez
Summary: The study evaluated the BSH activity in six probiotic strains, including five lactic acid bacteria and one probiotic yeast. Results showed that Lactobacillus plantarum DGIA1 and Saccharomyces boulardii exhibited excellent deconjugation activities against bile acids, with the yeast showing BSH activity for the first time. This finding highlights the potential of these probiotic strains in regulating cholesterol levels.
Article
Multidisciplinary Sciences
Matthew H. Foley, Sarah O'Flaherty, Garrison Allen, Alissa J. Rivera, Allison K. Stewart, Rodolphe Barrangou, Casey M. Theriot
Summary: Primary bile acids are metabolized by indigenous bacteria in the gastrointestinal tract, shaping host-microbiota interactions. Bacterial bile salt hydrolases cleave glycine or taurine from BAs, a crucial step for the production of secondary BAs. Lactobacilli harbor a diversity of BSHs, influencing their fitness and colonization in the gut, with enzymatic preferences and chemical features playing a role in determining toxicity during growth.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Carsten Nachtigall, Verena Hassler, Daniel Wefers, Harald Rohm, Doris Jaros
Summary: The study focuses on the techno-functionality of five dextrans from W. cibaria DSM14295, which were produced under different cultivation conditions. The dextrans differ in molecular mass, intrinsic viscosity, degree of branching, side chain length, and architecture. The stiffness of acid gels spiked with these dextrans increased linearly with dextran concentration. Dextrans from W. cibaria DSM14295 have a high potential due to their high production yield and ability to be tailored by fermentation conditions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Fisheries
Silas F. Eto, Dayanne C. Fernandes, Michelli I. G. Funnicelli, Joao V. C. Alecrim, Pedro G. Souza, Fabiola C. A. Carvalho, Marco A. A. Belo, Joao M. Pizauro
Summary: This study provides the first description of the ability of Nile tilapia microglia to form extracellular traps (ETs) in response to Weissella cibaria infection. The findings show that ETs formation occurred 6 hours after stimulation with lipopolysaccharide, shedding light on the mechanism of ETs formation in teleost fish microglia and W. cibaria's ability to infect these cells.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Microbiology
Ayeon Kwon, Young-Seo Park
Summary: The study confirms that synbiotics prepared by combining lactic acid bacteria with potential probiotic strains and glucooligosaccharides can significantly enhance immunostimulatory activity.
Article
Food Science & Technology
Guangqiang Wang, Huaning Yu, Xin Feng, Hongyu Tang, Zhiqiang Xiong, Yongjun Xia, Lianzhong Ai, Xin Song
Summary: This study explored the importance of different bsh genes in the bile salt resistance of L. plantarum AR113, revealing that bsh 1 and bsh 3 genes are critical for BSH activity and preferentially degrade glycine-conjugated bile salts. The results suggest that bsh 1 and bsh 3 are closely related to bile salt resistance, providing valuable insights for selecting strains with high tolerance to bile salts in a rational, high-throughput manner.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)