Article
Microbiology
Esa Abiso Godana, Qiya Yang, Lina Zhao, Xiaoyun Zhang, Jizhan Liu, Hongyin Zhang
Summary: The study found that Pichia anomala induced with chitosan significantly increased enzymatic activities in table grapes, improving their defense against blue mold disease caused by Penicillium expansum. Furthermore, the induction of yeast with chitosan also enhanced its bio-control efficacy against the pathogen, showing potential for the use of yeasts as bio-pesticides in the future.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Applied
Yemei Sun, Linlin Shang, Xiaoshuang Xia, Di Meng, Yun Ren, Jiaqi Zhang, Man Yao, Xinghua Zhou, Yun Wang
Summary: Chitosan treatment affected the transcriptome and physiological pathways of Penicillium expansum, with up-regulation of the gene encoding clathrin adaptor protein mu-subunit (PeCAM) involved in clathrin-dependent endocytosis. Deletion of PeCAM resulted in morphological changes in fungal cells and reduced sensitivity to chitosan, indicating that chitosan uptake is mediated by clathrin-dependent endocytosis and plays a crucial role in its antifungal activity.
CARBOHYDRATE POLYMERS
(2021)
Article
Agronomy
Yining Huang, Liguang Gao, Ming Lin, Ting Yu
Summary: The modified safety-secured yeast showed great potential in overexpressing antimicrobial peptides, improving biocontrol ability. The recombinant strains demonstrated significant suppression of pathogens and could be promising biological agents in postharvest applications.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Microbiology
Monica Gandia, Anant Kakar, Moises Giner-Llorca, Jeanett Holzknecht, Pedro Martinez-Culebras, Laszlo Galgoczy, Florentine Marx, Jose F. Marcos, Paloma Manzanares
Summary: This study evaluated the potential application of different antifungal proteins (AFPs) in controlling Penicillium decay, and found that PAFB was the most potent AFP against P. digitatum, P. italicum, and P. expansum. The combination of different AFPs did not result in an increase in disease control efficacy.
Article
Mycology
Nayyereh Alimadadi, Zahra Pourvali, Shaghayegh Nasr, Seyed Abolhassan Shahzadeh Fazeli
Summary: Blue mold decay caused by Penicillium expansum is a major postharvest disease of grapes, resulting in significant economic losses. This study aimed to identify potential yeast strains for biological control of blue mold on table grapes. Six yeast strains (Coniochaeta euphorbiae, Auerobasidium mangrovei, Tranzscheliella sp., Geotrichum candidum, Basidioascus persicus, and Cryptococcus podzolicus) showed significant inhibitory activity against P. expansum and G. candidum was the most efficient biocontrol agent.
Article
Food Science & Technology
Jiayu Zhou, Weifeng Gong, Tingting Tu, Jiaqi Zhang, Xiaoshuang Xia, Luning Zhao, Xinghua Zhou, Yun Wang
Summary: In this study, the researchers identified 212 and 268 candidate effector protein genes of P. expansum during infection of apple fruits by using RNA-Seq analysis and computational prediction. One of the candidate genes, Peclg, was induced significantly during the pathogen-host interaction. Knockout of Peclg gene resulted in reduced conidial production and germination, increased sensitivity to salt stress, SDS and Congo red, and decreased pathogenicity and infectious growth on apple fruits.
Article
Food Science & Technology
Lucia Chaves Simoes, Ana Filipa Amaral Chaves, Manuel Simoes, Nelson Lima
Summary: This study evaluates the association between bacteria and filamentous fungi in biofilm formation and tolerance to chlorination. The results showed that the association of bacteria and filamentous fungi favored biofilm production and reduced susceptibility to disinfection. The presence of filamentous fungi also increased the removal and inactivation effects of disinfectants.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Agronomy
Xiangfeng Zheng, Lingli Zheng, Fangping Xia, Jiang Li, Wenyun Zhou, Lei Yuan, Shengqi Rao, Zhengquan Yang
Summary: The yeast Kluyveromyces marxianus was found to have antagonistic ability against pathogens and can reduce the incidence of blue mold disease and patulin accumulation in apples. It achieves this through competitive advantage, production of antibacterial substances, and activation of plant hormone signal transduction.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Microbiology
Holly P. Bartholomew, Dianiris Luciano-Rosario, Michael J. Bradshaw, Verneta L. Gaskins, Hui Peng, Jorge M. Fonseca, Wayne M. Jurick, Essaid Ait Barka, Tomislav Cernava, Beibei Ge
Summary: This study investigates two Penicillium chrysogenum isolates as potential biocontrol agents against Penicillium expansum in apples. The results show that these isolates can effectively reduce decay incidence and severity in apples, without showing antagonism with Penicillium expansum. These findings contribute to the development of novel decay intervention strategies for managing postharvest diseases.
Article
Food Science & Technology
Ante Loncaric, Bojan Sarkanj, Ana-Marija Gotal, Marija Kovac, Ante Nevistic, Goran Fruk, Martina Skendrovic Babojelic, Jurislav Babic, Borislav Milicevic, Tihomir Kovac
Summary: The study examined the resistance of traditional and conventional apple cultivars to Penicillium expansum and found a positive correlation between patulin content and polyphenolic compounds such as catechin, epicatechin, and gallic acid in apples.
Article
Plant Sciences
Xuemei Zhang, Yuanyuan Zong, Di Gong, Lirong Yu, Edward Sionov, Yang Bi, Dov Prusky
Summary: This study investigated the regulatory effects of NADPH oxidase (Nox) genes on the growth and pathogenicity of Penicillium expansum in apple fruits. Mutants of Delta PeNoxA, Delta PeNoxR, and Delta PeRacA genes showed differential effects on the colonization process, with PeRacA demonstrating the strongest regulatory effect. Analysis of ROS content in the mycelium confirmed the functionality of the Nox mutations.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Food Science & Technology
Badiaa Essghaier, Marwa Smiri, Hiba Sehimi, Amel ben Jalloul, Mohamed Faouzi Zid, Najla Sadfi-Zouaoui
Summary: This study found that two vanadium oxalate compounds can effectively inhibit the growth and spore germination of Penicillium expansum and Penicillium italicum, reduce biofilm and patulin production, and affect mycelium morphogenesis.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2022)
Article
Agronomy
Kaili Wang, Xiangfeng Zheng, Yingying Su, Yuchun Lu, Qiya Yang, Yu Shi, Boen Lanhuang, Xiaoyun Zhang, Lina Zhao, Esa Abiso Godana, Solairaj Dhanasekaran, Guillaume Legrand Ngolong Ngea, Hongyin Zhang
Summary: This study identified the essential gene PeBgl1 in Penicillium expansum, which is upregulated during apple infection and affects colony morphology and fungal growth. The PeBgl1 gene also decreases the pathogenicity of P. expansum in apples.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Agronomy
Meiqiu Xu, Esa Abiso Godana, Solairaj Dhanasekaran, Xiaoyun Zhang, Qiya Yang, Lina Zhao, Hongyin Zhang
Summary: Penicillium expansum causes economic losses by causing blue mold disease in pears during storage and transportation. The defense mechanisms of pears against P. expansum are not well understood, limiting the progress in control strategies. This study used transcriptome and proteome approaches to evaluate the defense mechanisms of pears against P. expansum and identified significantly enriched genes/proteins related to metabolic processes and plant hormones. The findings contribute to the development of strategies for controlling fungal diseases.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Plant Sciences
Jiaqi Zhang, Di Meng, Xiaoshuang Xia, Yemei Sun, Luning Zhao, Xinghua Zhou, Yun Wang
Summary: In this study, researchers found that the effector protein PeSCP secreted by Penicillium expansum plays an important role in the virulence of the fungus on apple fruit. Deletion of the PeSCP gene resulted in reduced conidiation, germination, and affected fungal growth and morphology. The knockout also led to altered tolerance to environmental stresses and greatly reduced virulence of P. expansum on apple fruit.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2022)
Review
Food Science & Technology
Kaili Wang, Guillaume Legrand Ngolong Ngea, Esa Abiso Godana, Yu Shi, Boen Lanhuang, Xiaoyun Zhang, Lina Zhao, Qiya Yang, Siyun Wang, Hongyin Zhang
Summary: One of the major challenges in postharvest apple deterioration is the blue mold caused by Penicillium expansum, which results in significant economic losses. Current physical control methods cannot fully inhibit the growth of P. expansum, therefore alternative methods are needed. Research has shown that P. expansum infection not only enhances pathogenicity, but also suppresses the expression of host defense genes. With the advancements in omics technology, new ways to investigate and control P. expansum are expected.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Agronomy
Xiaoyun Zhang, Xueli Yan, Wanying Hu, Solairaj Dhanasekaran, Guillaume Legrand Ngolong Ngea, Esa Abiso Godana, Xiangyu Gu, Lina Zhao, Hongyin Zhang
Summary: Infection by Fusarium proliferatum increased soluble sugar concentration and titratable acidity of green asparagus initially before decreasing to levels lower than the control. Key enzyme activities related to physiological metabolism also increased initially in pathogen-infected asparagus before declining below control levels.
NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE
(2022)
Article
Food Science & Technology
William Tchabo, Giscard Kuate Kaptso, Guillaume Legrand Ngolong Ngea, Kenuo Wang, Guifeng Bao, Yongkun Ma, Xiangyang Wang, Carl Moses Mbofung
Summary: This study assessed the effects of carrier materials and drying techniques on the digestibility and antioxidant activity of mulberry leaf extract. The results showed that encapsulation enhanced bioaccessibility but had negative effects on bioefficiency and bioavailability. Furthermore, carriers had a major impact on digestion and antioxidative activity, while drying techniques mostly affected the bioaccessibility and bioavailability of flavonols.
Article
Biotechnology & Applied Microbiology
Meilin Wei, Solairaj Dhanasekaran, Guillaume Legrand Ngolong Ngea, Esa Abiso Godana, Xiaoyun Zhang, Qiya Yang, Xiangfeng Zheng, Hongyin Zhang
Summary: The study found that Cryptococcus podzolicus Y3 efficiently eliminated ochratoxin A (OTA) and converted it into non-toxic ochratoxin A (OTa) in different media. The elimination substances produced by the strain were heat stable and involved intracellular proteins. Additionally, C. podzolicus Y3 also had the ability to degrade citrinin (CIT), although the efficiency was lower than single mycotoxin degradation.
BIOLOGICAL CONTROL
(2022)
Article
Biotechnology & Applied Microbiology
Esa Abiso Godana, Xiaoyun Zhang, Wanying Hu, Lina Zhao, Xiangyu Gu, Hongyin Zhang
Summary: Our study revealed the activation of signal transduction pathways and changes in gene expression in asparagus treated with Yarrowia lipolytica. These changes enhanced asparagus' disease resistance and provide insights for further investigation of the molecular mechanisms involved in the interaction between plants and antagonistic yeasts.
BIOLOGICAL CONTROL
(2022)
Article
Food Science & Technology
Meilin Wei, Solairaj Dhanasekaran, Esa Abiso Godana, Qiya Yang, Yuan Sui, Xiaoyun Zhang, Guillaume Legrand Ngolong Ngea, Hongyin Zhang
Summary: This study analyzed the whole-genome sequencing of Cryptococcus podzolicus Y3 and investigated the protein expression profile during ochratoxin A (OTA) degradation. The results provide important genomic information and reveal the toxicity and stress response mechanism of C. podzolicus Y3 during OTA degradation at the protein level.
Article
Agronomy
Meiqiu Xu, Esa Abiso Godana, Solairaj Dhanasekaran, Xiaoyun Zhang, Qiya Yang, Lina Zhao, Hongyin Zhang
Summary: Penicillium expansum causes economic losses by causing blue mold disease in pears during storage and transportation. The defense mechanisms of pears against P. expansum are not well understood, limiting the progress in control strategies. This study used transcriptome and proteome approaches to evaluate the defense mechanisms of pears against P. expansum and identified significantly enriched genes/proteins related to metabolic processes and plant hormones. The findings contribute to the development of strategies for controlling fungal diseases.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Agronomy
Yu Shi, Qiya Yang, Qidi Zhang, Qianhua Zhao, Esa Abiso Godana, Xiaoyun Zhang, Siqi Zhou, Hongyin Zhang
Summary: Microbial communities associated with plants play a vital role in plant growth regulation and disease control. This study investigated the effects of preharvest treatment with Aureobasidium pullulans S2 on the fungal and bacterial communities on tomato surfaces, and found that it significantly altered the microbial composition and potentially beneficial genera. Further research is needed to understand the functions of these taxa and utilize them for targeted microbial manipulation in fruits and vegetables.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Jamila A. Tuly, Haile Ma, Hossain M. Zabed, Quaisie Janet, Esa Abiso Godana, Gu Chen, John-Nelson Ekumah
Summary: To provide balanced nutrition to livestock, chicken feathers and soybean by-products were mixed and fermented using a Bacillus mutant strain. The resulting feed showed an increased content of essential amino acids, particularly methionine. The fermentation process also reduced malodorous chemicals and hazardous nitrogen. Overall, the mixed feed demonstrated better results compared to the other samples.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Chemistry, Applied
Qianhua Zhao, Yu Shi, Guillaume Legrand Ngolong Ngea, Xiaoyun Zhang, Qiya Yang, Qidi Zhang, Xiaoyang Xu, Hongyin Zhang
Summary: This study found that Wickerhamomyces anomalus can control the postharvest disease of kiwifruit. High-throughput sequencing technology revealed that the changes in fungal composition and structure in the microbial community were greater than those of bacteria after W. anomalus treatment. W. anomalus can colonize inside the fruit and regulate the composition of bacterial community to reduce pathogen abundance and maintain fruit health.
Article
Agronomy
Kaili Wang, Xiangfeng Zheng, Yingying Su, Yuchun Lu, Qiya Yang, Yu Shi, Boen Lanhuang, Xiaoyun Zhang, Lina Zhao, Esa Abiso Godana, Solairaj Dhanasekaran, Guillaume Legrand Ngolong Ngea, Hongyin Zhang
Summary: This study identified the essential gene PeBgl1 in Penicillium expansum, which is upregulated during apple infection and affects colony morphology and fungal growth. The PeBgl1 gene also decreases the pathogenicity of P. expansum in apples.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Chemistry, Applied
Yiran Wang, Qiya Yang, Esa Abiso Godana, Yu Zhang, Hongyin Zhang
Summary: In this study, the morphological changes of Penicillium expansum within apple wounds during the infection process were investigated. The study revealed that conidia swelled and secreted potential hydrophobin in 4 h, germinated in 8 h, and formed conidiophores in 36 h. The transcript accumulation of P. expansum in apple tissues and liquid culture at 12 h was also compared, revealing the up-regulation and down-regulation of genes related to the biosynthesis of substances and activation of pathways.
Article
Agronomy
Lina Zhao, Yali Zhou, Luyi Liang, Esa Abiso Godana, Xiaoyun Zhang, Xiangzheng Yang, Maoyu Wu, Yuanda Song, Hongyin Zhang
Summary: Microbial antagonists offer a fresh perspective on biological control of postharvest fruit diseases by altering microbial diversity. This study investigated the effects of Debaryomyces hansenii on postharvest diseases and quality of strawberries. The results showed that D. hansenii treatment inhibited decay and maintained higher ascorbic acid levels in strawberries. The treatment also changed the fungal community composition on the strawberries' surface, while maintaining bacterial and fungal populations that could suppress plant pathogens and reduce postharvest diseases.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Plant Sciences
Dhanasekaran Solairaj, Ngolong Ngea Guillaume Legrand, Qiya Yang, Jizhan Liu, Hongyin Zhang
Summary: This study investigated the infection process of C. cladosporioides and its impact on grape quality and defense-related enzymes. The results showed that C. cladosporioides effectively infects grapes at optimal temperature and wetness, completing its lifecycle within 48 hours. The infection also resulted in a decrease in grape quality and induced the activity of defense-related enzymes at certain time points.
HORTICULTURAL PLANT JOURNAL
(2022)