Article
Engineering, Environmental
Jian Lu, Yuxuan Zhang, Jun Wu, Jianhua Wang
Summary: This study investigated the impact of antimicrobial peptide intervention on antimicrobial resistance in aquaculture by analyzing the abundance of antibiotic resistance genes (ARGs) in various samples. The results showed that cecropin treatment significantly reduced the absolute abundance of ARGs, while antibiotic treatment had the opposite effect. Network analysis revealed that cecropin treatment could decrease the occurrence of multi-drug resistant bacteria and the correlation between genera and ARGs.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Infectious Diseases
Joan U. Okafor, Uchechukwu U. Nwodo
Summary: This study evaluated the antibiotic susceptibility and resistance genes of Pseudomonas aeruginosa in hospital wastewater. The findings revealed a high prevalence of multidrug-resistant Pseudomonas aeruginosa with various resistance genes, posing potential risks to environmental health.
Article
Engineering, Environmental
Lu-Xi He, Liang-Ying He, Fang-Zhou Gao, Min Zhang, Jun Chen, Wei-Li Jia, Pu Ye, Yu-Wei Jia, Bai Hong, Si-Si Liu, You-Sheng Liu, Jian-Liang Zhao, Guang-Guo Ying
Summary: Antibiotics are being used extensively in mariculture, leading to the spread of antibiotic resistance in the marine environment. This study investigated the pollution, distribution, and characteristics of antibiotics, antibiotic resistance genes (ARGs), and microbiomes. The findings revealed the presence of 20 antibiotics in Chinese coastal areas, with higher concentrations and more types of antibiotics detected in mariculture sites compared to control sites. Certain antibiotics, such as enrofloxacin, ciprofloxacin, and sulfadiazine, posed a high risk of resistance selection. The study also highlighted the presence of high-risk ARGs in certain bacterial phyla, suggesting a potential threat to human health.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Fisheries
Luu Quynh Huong, Nguyen Thi Bich Thuy, Nguyen Thi Lan Anh, Do Thi Thu Thuy, Dao Thi Ha Thanh, Pawin Padungtod
Summary: The total value of aquaculture production in Vietnam in 2015 was $4.26 billion, contributing 65% of total fisheries exports. Antibiotic use was common in livestock and aquaculture industries in Vietnam for treatment or growth stimulation. The study aimed to describe the rationale and pattern of antibiotic use in fish and shrimp farms.
AQUACULTURE REPORTS
(2021)
Article
Environmental Sciences
Valentina Silva, Ines Invencio, Carla Patricia Silva, Marta Otero, Diana L. D. Lima
Summary: This study successfully achieved the photocatalytic degradation of antibiotic oxolinic acid (OXA) under solar radiation by incorporating carbon quantum dots (CQDs) into titanium dioxide (TiO2). Among the thirteen materials tested, CQDs produced with citric acid and incorporated into TiO2 at 4% (w/w) showed the best performance, reducing the half-life time (t1/2) of OXA by 91%, 79%, and 85% in different matrices.
Article
Biotechnology & Applied Microbiology
Ahamed Basha Kusunur, Shanmukha Sai Mogilipuri, Dhamodher Moturu, Manikantha Benala, Murugadas Vaiyapuri, Satyen Kumar Panda, Joshy Chalil George, Madhusudana Rao Badireddy
Summary: This study investigated the tetracycline resistance potential of heterotrophic bacteria isolated from freshwater fin-fish culture ponds in Andhra Pradesh, India. The results showed that Lactococcus garvieae was the predominant bacteria with high tetracycline resistance, followed by Enterobacter spp., Lactococcus lactis, and Staphylococcus arlettae. The tet(A) gene was the major determinant of tetracycline resistance. The tet(HR) bacteria showed cross-resistance to furazolidone, co-trimoxazole, and enrofloxacin.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Infectious Diseases
Le Thanh Dien, Le Buu Ky, Bui The Huy, Muhammad Fadhlullah Mursalim, Pattanapon Kayansamruaj, Saengchan Senapin, Channarong Rodkhum, Ha Thanh Dong
Summary: This study identified a new lytic phage, pAh6.2TG, which showed high specificity and stability against multidrug-resistant Aeromonas hydrophila. In experimental challenges, phage treatment significantly improved survivability of Nile tilapia exposed to the pathogenic MDR A. hydrophila, while reducing bacterial concentrations in both water and fish body. These findings suggest that pAh6.2TG is an effective alternative to antibiotics for controlling MDR A. hydrophila in tilapia and possibly other freshwater fish species.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Review
Fisheries
Vijaya Kumar Deekshit, Biswajit Maiti, Ballamoole Krishna Kumar, Akshatha Kotian, Gillaine Pinto, Melba G. Bondad-Reantaso, Iddya Karunasagar, Indrani Karunasagar
Summary: The discovery and use of antibiotics in various sectors, including aquaculture, has led to concerns about the indiscriminate use of antibiotics and the emergence of antimicrobial resistance. It is important to study the transmission and resistance mechanisms of fish pathogens in aquaculture settings.
REVIEWS IN AQUACULTURE
(2022)
Review
Fisheries
Le Thanh Dien, Thao Phuong Huynh Ngo, Thao V. Nguyen, Pattanapon Kayansamruaj, Krishna R. Salin, Chadag Vishnumurthy Mohan, Channarong Rodkhum, Ha Thanh Dong
Summary: Inland aquaculture, particularly in low and middle-income countries, plays a crucial role in global food security, but its sustainable development is hindered by disease epidemics, especially those caused by bacteria such as Aeromonas species. This article provides an overview of motile Aeromonas infections, antibiotic use, and antimicrobial resistance, as well as non-antibiotic approaches to control these infections. It discusses the current state of knowledge, limitations, and future research perspectives, including vaccines, microencapsulated synbiotics, exogenous metabolites, and novel lytic bacteriophage cocktails.
REVIEWS IN AQUACULTURE
(2022)
Article
Infectious Diseases
Maria Garza, Chadag Mohan, Lucy Brunton, Barbara Wieland, Barbara Hasler
Summary: This study aims to develop a typology to classify and characterize interventions to reduce antimicrobial use in aquaculture and identify points of action. The most common interventions to address antimicrobial use and resistance were legislative and regulatory frameworks and voluntary instruments. Specific interventions targeting antimicrobial use and resistance were scarce. The presented typology can guide evaluation of interventions that promote responsible antimicrobial use and inform the design of future interventions.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Infectious Diseases
Katia Iskandar, Jayaseelan Murugaiyan, Dalal Hammoudi Halat, Said El Hage, Vindana Chibabhai, Saranya Adukkadukkam, Christine Roques, Laurent Molinier, Pascale Salameh, Maarten Van Dongen
Summary: The history and challenges of antibiotic discovery, as well as the need for diversity and innovation in tackling antimicrobial resistance (AMR), are described in this review. It emphasizes the public health concerns of acquired resistance caused by anthropogenic use of antibiotics and the global spread of AMR at an alarming rate. The lack of new leads in the current pipeline and the need to address resistance to old and novel antibiotics are also highlighted.
Review
Biochemistry & Molecular Biology
Daria Nicolosi, Giulio Petronio Petronio, Stefano Russo, Maria Di Naro, Marco Alfio Cutuli, Claudio Russo, Roberto Di Marco
Summary: The overuse and misuse of antibiotics have led to the emergence and spread of multidrug-resistant (MDR), extensively drug-resistant (XDR), and pan-drug-resistant (PDR) bacteria strains. To preserve the effectiveness of these drugs, it is crucial to use them appropriately. Innovative approaches are being investigated to improve drug treatments and reduce drug resistance. Global guidelines have been developed to optimize prescriptions and reduce the environmental spread of these life-saving molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Public, Environmental & Occupational Health
Alicia DeRosa, Michael T. Carter, Bethany A. Wattengel, Alan J. Lesse, John A. Sellick, Kari A. Mergenhagen
Summary: This study evaluated susceptibility trends in antimicrobials used for treatment of UTIs in the Veteran population in the United States, showing a downward trend in susceptibility for certain antibiotics. Therefore, empiric therapy for Veterans with UTIs should be based on local susceptibility patterns.
AMERICAN JOURNAL OF INFECTION CONTROL
(2021)
Editorial Material
Fisheries
Felipe C. Cabello, Henry P. Godfrey
Summary: Dr. Avendano-Herrera's comments on our review are mostly marginal and do not weaken our hypothesis. We believe that infections caused by P. salmonis can be prevented by improving husbandry practices, and that excessive use of antimicrobials is detrimental to health.
Article
Environmental Sciences
Lu-Xi He, Liang-Ying He, Fang-Zhou Gao, Dai-Ling Wu, Pu Ye, Yu-Xiao Cheng, Zi-Yin Chen, Li-Xin Hu, You-Sheng Liu, Jun Chen, Guang-Guo Ying
Summary: The study found various antibiotic residues in grouper mariculture systems in Hainan province, China, with fluoroquinolones and tetracyclines being dominant. The detected antibiotics in the mariculture water were significantly higher than in the original seawater. Certain antibiotics in the water and sediment pose high resistance selection risks.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Fisheries
Raja Swaminathan Thangaraj, Sundar Raj Nithianantham, Lekshmi Narendrakumar, Tina Kollannoor Johny, Neeraj Sood, Pravata Kumar Pradhan, Kuldeep Kumar Lal
Summary: The experimental results showed that Indian cichlids are not susceptible to TiLV infection, as confirmed by the absence of clinical signs or TiLV-specific amplification in both in vivo and in vitro tests.
Article
Fisheries
Prasannan Geetha Preena, Thaliyil Veetil Arun Kumar, Tina Kollannoor Johny, Arathi Dharmaratnam, Thangaraj Raja Swaminathan
Summary: The rapid and sensitive RPA-LFD assay developed in this study provides a simple and efficient method for sensitive diagnosis of CyHV-2 in goldfish within 20 minutes, with a high detection limit. This assay is easy to use, quick, and sensitive, making it suitable for point-of-care diagnosis, especially in resource-limited settings.
AQUACULTURE INTERNATIONAL
(2022)
Article
Biology
Prasannan Geetha Preena, Arathi Dharmaratnam, Thangaraj Raja Swaminathan
Summary: This study isolated several bacteria from diseased goldfish and identified Edwardsiella tarda as the real causative agent of the disease outbreak. Experimental challenge studies showed that this bacterium was highly pathogenic to goldfish and exhibited multiple antimicrobial resistance. Therefore, proper surveillance and diagnostic methods are needed to address the threat of this disease.
Article
Virology
Dev Kumar Verma, Neeraj Sood, Anutosh Paria, T. R. Swaminathan, C. V. Mohan, K. V. Rajendran, P. K. Pradhan
Summary: The tilapia lake virus (TiLV), a highly infectious viral pathogen, poses a major threat to the global tilapia industry. This study investigated the genomic evolution of TiLV and found that reassortment, selection, and mutation play important roles in its evolution. The results highlight the dominant force of reassortment in the emergence of this highly infectious segmented RNA virus.
Article
Parasitology
Krishna Remya, Y. Ajith, J. Parvathy, Varuna P. Panicker, P. Preena, V. R. Ambily, Thankachan Ancy, Francis Anjaly, N. Madhavan Unny, Usha Narayana Pillai
Summary: This report describes a case of Theileria spp. infection in a lactating goat, which presented with acute insulin-responsive hyperglycemia and hypocalcemia, and achieved rapid clinical recovery through intensive therapy. The case highlights the importance of investigating the metabolic status of animals with theileriosis for better clinical responses.
VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS
(2022)
Article
Fisheries
Soumya Balakrishnan, Ahna Ameer, Sowmya Pazhur Mohandas, Ambadi Kannan Maliyekkal Sajeevan, Anoop Bhaskaran Sathyabhama, Bright Singh
Summary: The study demonstrates that pyocyanin is an effective and environmentally friendly aquaculture drug for controlling Vibrio spp. in RAS-based shrimp culture without impeding nitrification by nitrifying bacteria.
AQUACULTURE INTERNATIONAL
(2022)
Article
Fisheries
Ancy Thomas, Naduvilamuriparampu Saidumuhammed Sudheer, Viswanath Kiron, Bright Singh Issac Singh, Rangarajan Badri Narayanan
Summary: This paper investigates the effectiveness of the major antigenic proteins VP24 and VP28 from white spot syndrome virus (WSSV) in inducing an immune response in shrimp. The recombinant proteins were mass-produced and orally administered to shrimp, resulting in the upregulation of several biodefense genes. The study reveals the antigenic properties of VP24 and VP28 and their ability to elicit an immune response after oral administration.
AQUACULTURE RESEARCH
(2022)
Article
Cell Biology
Manoj K. Yadav, Aakriti Rastogi, Dev K. Verma, Anutosh Paria, Basdeo Kushwaha, Gaurav Rathore, T. Raja Swaminathan, Pravata K. Pradhan, Neeraj Sood
Summary: A continuous cell line LCF has been successfully established from the caudal fin of Labeo calbasu. The LCF cells show optimal growth conditions and can be used for expression of foreign genes. It is also resistant to Tilapia lake virus (TiLV) infection.
CELL BIOLOGY INTERNATIONAL
(2022)
Review
Biochemical Research Methods
Tina Kollannoor Johny, Thangaraj Raja Swaminathan, Neeraj Sood, Pravata Kumar Pradhan, Kuldeep Kumar Lal
Summary: This review manuscript provides an overview of disease diagnosis techniques in aquaculture, including conventional techniques and immunological methods. It emphasizes the application of molecular techniques and novel approaches, discussing their principles, strengths, weaknesses, and variations. It also explores the potential of future technologies to revolutionize disease diagnosis.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)
Review
Biotechnology & Applied Microbiology
Soumya Balakrishnan, I. S. Bright Singh, Jayesh Puthumana
Summary: This review discusses the obstacles and importance of developing marine molluscan cell lines, focusing on the advancements and future trends in the past decade.
Article
Microbiology
Das Prabina, T. Raja Swaminathan, Sowmya P. Mohandas, J. C. Anjana, K. Manjusha, P. G. Preena
Summary: This study investigated the prevalence of antimicrobial-resistant vibrios among infected shrimps from two brackish water-cultured farms. The results showed that the vibrios exhibited resistance to multiple antibiotics, especially tetracyclines, beta-lactams, and cephalosporins. The presence of plasmid-borne antimicrobial resistance among the isolates is a concern for public health and the food industry.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Fisheries
Arathi Dharmaratnam, Arun Sudhagar, Thangaraj Raja Swaminathan
Summary: In this experiment, a heat-inactivated CyHV-2 vaccine was developed and evaluated in goldfish. The vaccine showed significant upregulation of immune genes and provided better protection against CyHV-2 in the challenged fish.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Microbiology
Arun Sudhagar, Nithianantham Sundar Raj, Sowmya Pazhur Mohandas, Shaji Serin, Konnoth Kuttappan Sibi, Nandiath Karayi Sanil, Thangaraj Raja Swaminathan
Summary: Freshwater velvet disease, caused by the dinoflagellate parasite Piscinoodinium sp., has been reported in tropical and subtropical fishes and can cause devastating losses. However, there is limited molecular information about this parasite. In this study, Piscinoodinium sp. was found to be responsible for 100% mortality in captive-bred Arulius barb. The molecular information generated from this study could serve as a baseline for further research on the diversity of this parasite in India.
Article
Virology
Dominic Vithayathil Diya Dominic, Thavarool Puthiyedathu Sajeevan, Ahna Ameer, Abdulkhader Muneer, Linu Eldho, Bhaskaran Sathyabhama Anoop, Puthumana Jayesh, Valsamma Joseph, Rosamma Philip, Isaac Sarojini Bright Singh
Summary: Infectious hypodermal hematopoietic necrosis virus (IHHNV/PstDVI) was successfully isolated and propagated in a hybrid shrimp-insect cell line PmLyO-Sf9. Cytopathic changes such as clustering, enlargement, syncytium formation, and cell death were observed in the infected cell line, indicating viral replication. The purified virus was found to be infectious both in vitro and in vivo, providing insights for studying the molecular mechanism of IHHNV infection and developing antiviral strategies in aquaculture.
ARCHIVES OF VIROLOGY
(2023)
Article
Biology
Lekshmi Narendrakumar, Arun Sudhagar, Prasannan G. Preena, Sundar R. Nithianantham, Sowmya P. Mohandas, Thangaraj Raja Swaminathan
Summary: This study investigated the causative agents of chronic infection in betta fish from a private farm in Kerala, India, and found various bacteria and the presence of Mycobacterium marinum. The study recommends frequent monitoring of bacterial pathogens and antibiotic resistance in betta farms, as well as regular inspection of fish tanks.