Article
Environmental Sciences
Bugao Guan, Hongbo Li, Jian Yao, Jinbao Guo, Fei Yu, Guangrun Li, Benhai Wan, Jun Ma, Desong Huang, Lu Sun, Yan Chen
Summary: The study finds that CCL3 can be derived from TAMs and tumor cells of colon adenocarcinoma (COAD), and it promotes the migration and invasiveness of COAD cells by activating the Akt signaling pathway through binding to CCR5. High expression of CCL3/CCR5 is closely associated with poor prognosis of COAD patients.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Oncology
Mengmeng Wang, Mingjun Gao, Yong Chen, Jun Wu, Xiaolin Wang, Yusheng Shu
Summary: This study found that PLCD3 is highly expressed in ESCC tissues and cell lines, and its expression is correlated with pathological stage and lymphatic metastasis. PLCD3 knockdown inhibits cell proliferation, migration, and invasion, promotes apoptosis, and arrests the cell cycle in the G1 phase. PLCD3 overexpression, on the other hand, promotes cell proliferation, migration, and invasion. Xenograft models also suggest that PLCD3 promotes tumorigenesis in ESCC. Finally, overexpression of PLCD3 activates the PI3K/AKT/P21 signaling pathway.
Article
Chemistry, Multidisciplinary
Hui Shi, Qianqian Ju, Yinting Mao, Yuejun Wang, Jie Ding, Xiaoyu Liu, Xin Tang, Cheng Sun
Summary: The study reveals that TAK1 downregulation in esophageal tumors inhibits cell proliferation by phosphorylating RASSF9 and blocking the RAS/MEK/ERK axis, suggesting a potential therapeutic target for esophageal cancer treatment.
Article
Biochemistry & Molecular Biology
Zongfei Li, Yunzhi Pan, Jiayi Yao, Yingyin Gao, Yulan Qian, Minxue Zheng, Sai Ma
Summary: Our study shows that FBXW7 loss of function is crucial in esophageal cancer, but its mechanism in promoting the disease remains unclear. By screening in esophageal squamous cell carcinoma (ESCC), we identified ANXA2 as a potential target of FBXW7. In vitro experiments demonstrated that FBXW7 loss of function could restore ANXA2 expression and enhance the malignant characteristics of ESCC cells. Additionally, ANXA2 up-regulation was found to activate the ERK pathway in ESCC. Moreover, we observed high expression of ANXA2 in ESCC tissues, which was associated with poor tumor staging. Furthermore, a combination regimen targeting ANXA2 and the ERK pathway showed promising inhibitory effects on xenograft tumor growth in vivo. Overall, this study provides evidence that FBXW7 loss of function promotes esophageal cancer through ANXA2 overexpression, suggesting a potential target for ESCC treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yunlong Jia, Cong Tian, Hongyan Wang, Fan Yu, Wei Lv, Yuqing Duan, Zishuo Cheng, Xuexiao Wang, Yu Wang, Tianxu Liu, Jiali Wang, Lihua Liu
Summary: The study revealed that NORAD upregulates MTDH by sponging miR-224-3p, promoting CDDP resistance and progression in ESCC. This suggests the potential of NORAD as a therapeutic target for ESCC patients receiving CDDP-based chemotherapy.
Article
Biotechnology & Applied Microbiology
Qingfeng Ni, Hai Zhang, Xiaoli Shi, Xiangcheng Li
Summary: Cholangiocarcinoma is an aggressive malignant tumor disease with increasing incidence and mortality. Identifying specific biomarkers for cholangiocarcinoma treatment and diagnosis is urgent. Recent studies have highlighted the importance of lncRNAs in cancer and their downstream mechanism with miRNA networks. This study aimed to investigate the role of lncRNA HCG18 and its downstream mechanism in cholangiocarcinoma tumor progression.
CANCER GENE THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Yung-Chieh Tsai, Tian-Ni Kuo, Yu-Ying Chao, Pei-Rong Lee, Ruei-Ci Lin, Xiao-Yi Xiao, Bu-Miin Huang, Chia-Yih Wang
Summary: Testes play a crucial role in the development of the male reproductive system. This study demonstrates that the PDGF-AA/PDGFR-alpha signaling pathway regulates Leydig cell development and function through primary cilia. The activation of AKT and ERK signaling around the base of primary cilia promotes Leydig cell growth, migration, and invasion. Understanding the role of primary cilia and downstream signaling in testicular physiology provides important insights into male reproductive health.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Oncology
Md Rashedunnabi Akanda, Jee Soo Park, Myung-Giun Noh, Geun-Hyoung HA, Young Sook Park, Jae-Hyuk Lee, Kyung-Sub Moon, Chung Kwon Kim, Kyung-Hwa Lee
Summary: TACC3 contributes to gastric tumorigenesis by promoting EMT via the ERK/Akt/cyclin D1 signaling pathway. The correlation between TACC3 expression and multiple clinicopathological variables implies that its effective therapeutic targeting in GC will depend on the tumor subtype.
ANTICANCER RESEARCH
(2021)
Article
Cell Biology
Ke-Fan Pan, Yu-Cheng Liu, Michael Hsiao, Tsu-Yao Cheng, Kuo-Tai Hua
Summary: Naa10p plays an oncogenic role in promoting esophageal cancer metastasis, with higher expression correlating with poorer patient survival. Transcription of NAA10 is regulated by the critical oncogene c-Myc. The enzymatic activity of Naa10p, along with Naa15p, inhibits the secretion of the protease inhibitor PAI1. These findings establish the significance of Naa10p in driving metastasis in esophageal cancer, suggesting its potential use as a prognostic biomarker and therapeutic target.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Juanli Ji, Zhongze Wang, Wei Sun, Zekun Li, Huarui Cai, Erhu Zhao, Hongjuan Cui
Summary: Cynaroside (Cy), a flavonoid glycoside compound found in plants, has shown anticancer activity in various cancers, but its role in gastric cancer has not been extensively reported. This study confirmed that Cy can inhibit cell growth, migration, invasion, and tumorigenesis in gastric cancer by blocking the MET/AKT/mTOR axis. The findings suggest that Cy may be a potential drug for the treatment of gastric cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Chuanhong Zhong, Bei Tao, Xianglong Li, Wei Xiang, Lilei Peng, Tangming Peng, Ligang Chen, Xiangguo Xia, Jian You, Xiaobo Yang
Summary: This study identified the expression pattern of lncRNA HOXA-AS2 in glioma and revealed its role in immune tolerance and glioma progression. The results showed that lncRNA HOXA-AS2 upregulated KDM2A expression by preventing miR-302a from binding to its 3 ' untranslated region. This led to the promotion of regulatory T (T-reg) cell proliferation and immune tolerance, potentially through the activation of the KDM2A/JAG1 axis.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Li Wang, Qiao-Feng Zhao, Bing-Bing Yang, Hui-Jie Liang, Xian-E Zhang, Xiao-Yan Zhang, Wan-Jing Yang, Zhi-Yu Guo, Xin Xu, Fang Tian, Qing-Hua Wu
Summary: In this study, it was found that EphA7 expression was upregulated in ESCC cell lines with high STAT3 activation, and immunofluorescence showed co-localization of EphA7 with phospho-STAT3 in ESCC cells. The protein expression and transcription of EphA7 were mediated by the activation of STAT3, and EphA7 enhanced the cell proliferation of ESCC. Silence of EphA7 significantly suppressed ESCC tumor growth and abolished STAT3 activation-derived cell proliferation of ESCC.
Article
Medicine, Research & Experimental
Tianxiang Ni, Qian Zhang, Yan Li, Caiyi Huang, Tingting Zhou, Junhao Yan, Zi-Jiang Chen
Summary: Increasing evidence suggests a close relationship between non-coding RNAs and recurrent implantation failure. Circular RNA circSTK40 plays a role in inhibiting decidualization and promoting cell survival in endometrial stromal cells, possibly through modulating the degradation of proteins such as HSP90 and CLU, leading to activation of the AKT pathway and downregulation of FOXO1 expression. Targeted treatments based on these findings could potentially benefit patients with low endometrial receptivity.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Oncology
Jianguo Wang, Shizhong Cai, Qianwei Xiong, Deyu Weng, Qian Wang, Zhourui Ma
Summary: Phosphoinositide-3-kinase regulatory subunit 2 (PIK3R2) is highly expressed in melanoma and exacerbates its malignancy by activating the PI3K/AKT/NF-kappa B signaling pathway, suggesting PIK3R2 as a potential therapeutic target for melanoma.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2023)
Article
Surgery
Limin Ma, Hua Li, Yanmin Lin, Geng Wang, Qiangzhou Xu, Yuping Chen, Ke Xiao, Xuguang Rao
Summary: In this study, circDUSP16 was found to regulate TKTL1 expression by sponging miR-497-5p in esophageal squamous cell carcinoma, suppressing cell growth, invasion, and glycolysis. Overexpression of miR-497-5p inhibited the progression of ESCC cells under hypoxia conditions. These findings shed light on the potential therapeutic targets for ESCC treatment.
JOURNAL OF SURGICAL RESEARCH
(2021)
Article
Pathology
Masataka Fujikawa, Yu-ichiro Koma, Masayoshi Hosono, Naoki Urakawa, Kohei Tanigawa, Masaki Shimizu, Takayuki Kodama, Hiroki Sakamoto, Mari Nishio, Manabu Shigeoka, Yoshihiro Kakeji, Hiroshi Yokozaki
Summary: The overexpression of CCL1 in TAM-like macrophages promotes ESCC progression by enhancing cell motility, while the expression of CCR8 receptor on ESCC cell surface also plays a significant role. These interactions suggest novel therapeutic targets for treating ESCC.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Medicine, Research & Experimental
Hiroki Sakamoto, Yu-ichiro Koma, Nobuhide Higashino, Takayuki Kodama, Kohei Tanigawa, Masaki Shimizu, Masataka Fujikawa, Mari Nishio, Manabu Shigeoka, Yoshihiro Kakeji, Hiroshi Yokozaki
Summary: The study reveals that PAI-1 derived from cancer-associated fibroblasts promotes invasion of esophageal squamous cell carcinoma cells and migration of macrophages through LRP1. High expression of PAI-1 and/or LRP1 is associated with poor prognosis in ESCC patients, suggesting PAI-1/LRP1 axis could be a potential target for cancer therapy.
LABORATORY INVESTIGATION
(2021)
Review
Cell Biology
Manabu Shigeoka, Yu-ichiro Koma, Mari Nishio, Masaya Akashi, Hiroshi Yokozaki
Summary: This review highlights the significance of macrophages in oral carcinogenesis and proposes studying the alteration of macrophage infiltrating compartments as a novel approach for gaining insights into cancer prevention and early detection.
Article
Oncology
Masaki Shimizu, Yu-ichiro Koma, Hiroki Sakamoto, Shuichi Tsukamoto, Yu Kitamura, Satoshi Urakami, Kohei Tanigawa, Takayuki Kodama, Nobuhide Higashino, Mari Nishio, Manabu Shigeoka, Yoshihiro Kakeji, Hiroshi Yokozaki
Summary: In this study, we found that MT2A plays a crucial role in the progression of ESCC, affecting the migration and invasiveness of tumor cells in CAF-like cells and cancer cells. MT2A and IGFBP2 may serve as potential novel therapeutic targets in ESCC.
Article
Oncology
Manabu Shigeoka, Yu-ichiro Koma, Takayuki Kodama, Mari Nishio, Masaya Akashi, Hiroshi Yokozaki
Summary: This study revealed that tongue cancer cell-derived CCL20 induced by interaction with macrophages promotes CD163 expression on macrophages.
FRONTIERS IN ONCOLOGY
(2021)
Article
Gastroenterology & Hepatology
Kei Matsumoto, Shinwa Tanaka, Takashi Toyonaga, Nobuaki Ikezawa, Mari Nishio, Masanao Uraoka, Tomoatsu Yoshihara, Hiroya Sakaguchi, Hirofumi Abe, Tetsuya Yoshizaki, Madoka Takao, Toshitatsu Takao, Yoshinori Morita, Hiroshi Yokozaki, Yuzo Kodama
Summary: This study found that patients in the Billroth II group with remnant gastric cancer at the anastomotic site had larger lesions with severe remnant gastritis compared to those in the non-Billroth II group. Endoscopic submucosal dissection in Billroth II patients involved longer operative times and a higher frequency of bleeding episodes.
CLINICAL ENDOSCOPY
(2022)
Article
Pathology
Kohei Tanigawa, Shuichi Tsukamoto, Yu-ichiro Koma, Yu Kitamura, Satoshi Urakami, Masaki Shimizu, Masataka Fujikawa, Takayuki Kodama, Mari Nishio, Manabu Shigeoka, Yoshihiro Kakeji, Hiroshi Yokozaki
Summary: Direct co-culture of esophageal squamous cell carcinoma (ESCC) cells and macrophages enhances the migration and invasion abilities of ESCC cells and promotes ESCC progression through the Akt and p38 MAPK signaling pathways. The expression and release of S100A8/A9 are significantly increased in co-cultured ESCC cells and play a crucial role in this process.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Cell Biology
Kazuyoshi Yanagihara, Yuki Iino, Hiroshi Yokozaki, Takanori Kubo, Tatsuya Oda, Takashi Kubo, Masayuki Komatsu, Hiroki Sasaki, Hitoshi Ichikawa, Takeshi Kuwata, Toshio Seyama, Atsushi Ochiai
Summary: This study successfully established 13 matched patient-derived tumor models of PDA, including PDX, PDCL, and CDX. By comparing the histological and molecular alterations in these model systems, researchers found that PDX tumors had similar molecular alterations to PDCL and CDX tumors, while OI tumors showed specific immunohistochemical profiles. These patient-derived tumor models provide useful tools for monitoring responses to antineoplastic agents and studying the biology of PDA.
Article
Oncology
Yu Kitamura, Yu-ichiro Koma, Kohei Tanigawa, Shuichi Tsukamoto, Yuki Azumi, Shoji Miyako, Satoshi Urakami, Takayuki Kodama, Mari Nishio, Manabu Shigeoka, Yoshihiro Kakeji, Hiroshi Yokozaki
Summary: High infiltration of tumor-associated macrophages (TAMs) is correlated with poor prognosis of esophageal squamous cell carcinoma (ESCC). In this study, direct co-culture of ESCC cells with macrophages induced interleukin 7 receptor (IL-7R) expression. Overexpression of IL-7R promoted ESCC cell survival and growth via the activation of the Akt and Erk1/2 signaling pathways. The IL-7/IL-7R axis also contributed to ESCC cell migration via the Akt and Erk1/2 signaling pathways. ESCC patients with high IL-7R expression in cancer nests exhibited poor disease-free survival.
Article
Oncology
Shuichi Tsukamoto, Yu-ichiro Koma, Yu Kitamura, Kohei Tanigawa, Yuki Azumi, Shoji Miyako, Satoshi Urakami, Masayoshi Hosono, Takayuki Kodama, Mari Nishio, Manabu Shigeoka, Hiroshi Yokozaki
Summary: Tumor-associated macrophages (TAMs) play a role in the progression of esophageal squamous cell carcinoma (ESCC). A direct co-culture system was used to study the interaction between ESCC cells and TAMs, revealing that matrix metalloproteinase 9 (MMP9) expression in ESCC cells was induced by direct co-culture with TAMs. MMP9 was associated with ESCC cell migration and invasion, and its expression was controlled by the Stat3 signaling pathway. Immunohistochemical analyses showed that MMP9 expression in cancer cells at the invasive front was related to high infiltration of M2-like TAMs and worse survival of patients. However, MMP9 expression in cancer stroma was not associated with any clinicopathological factors or patient prognoses.
Article
Oncology
Satoshi Urakami, Yu-ichiro Koma, Shuichi Tsukamoto, Yuki Azumi, Shoji Miyako, Yu Kitamura, Takayuki Kodama, Mari Nishio, Manabu Shigeoka, Hirofumi Abe, Yu Usami, Yuzo Kodama, Hiroshi Yokozaki
Summary: M2 macrophages contribute to the progression of oesophageal squamous cell carcinoma (ESCC) by promoting the proliferation and migration of Het-1A cells. This phenomenon is mediated by the mTOR-p70S6K signaling pathway activated by hypersecreted YKL-40 and OPN. YKL-40 and OPN also promote M2 polarization, proliferation, and migration of macrophages.
JOURNAL OF PATHOLOGY
(2023)
Article
Genetics & Heredity
Shihori Tanabe, Eger Boonstra, Taehun Hong, Sabina Quader, Ryuichi Ono, Horacio Cabral, Kazuhiko Aoyagi, Hiroshi Yokozaki, Edward J. Perkins, Hiroki Sasaki
Summary: This study analyzed the molecular networks of anti-cancer drugs such as cisplatin, carboplatin, oxaliplatin, and arsenic trioxide in various types of cancer to reveal the mechanism of drug resistance. The study also highlighted the significance of tumor microenvironment in the treatment of drug-resistant cancer.
Article
Surgery
Takeo Kimoto, Norio Kohno, Akiko Okamoto, Kyosuke Ota, Takafumi Tani, Takeshi Kondo, Mari Nishio
Summary: This case report presented a rare occurrence of breast cancer metastasis to the inguinal lymph nodes in a patient who had undergone multiple surgeries. The progression of the inguinal lymph node metastases was closely correlated with that of the primary breast cancer, with one potential cause being attributed to extensive previous abdominal operations.
SURGICAL CASE REPORTS
(2021)
Article
Medicine, Research & Experimental
Marijn A. Scheijde-Vermeulen, Lennart A. Kester, Liset Westera, Bastiaan B. J. Tops, Friederike A. G. Meyer-Wentrup
Summary: This study aimed to evaluate the feasibility of integrating state-of-the-art sequencing techniques and flow cytometry into the diagnostic workup of pediatric lymphoma. The results showed that this integration is not only feasible but also provides additional diagnostic information.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Enrico Berrino, Sara Erika Bellomo, Anita Chesta, Paolo Detillo, Alberto Bragoni, Amedeo Gagliardi, Alessio Naccarati, Matteo Cereda, Gianluca Witel, Anna Sapino, Benedetta Bussolati, Gianni Bussolati, Caterina Marchi
Summary: Formalin-fixed paraffin-embedded (FFPE) samples are crucial for tissue-based analysis in precision medicine, but the quality of these samples can affect the reliability of sequencing data. The use of acid-deprived fixatives guarantees the highest DNA preservation and sequencing performance, enabling more complex molecular profiling of tissue samples.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Roope A. Kallionpaa, Sirkku Peltonen, Kim My Le, Eija Martikkala, Mira Jaaskelainen, Elnaz Fazeli, Pilvi Riihila, Pekka Haapaniemi, Anne Rokka, Marko Salmi, Ilmo Leivo, Juha Peltonen
Summary: This study investigated the immune microenvironment of cutaneous neurofibromas (cNFs) in patients with neurofibromatosis 1 (NF1). The results showed that cNFs have substantial populations of T cells and macrophages, which may be tumor-specific. T cell populations in cNFs were found to be different from those in the skin, and cNFs exhibited lower expression of proteins related to T cell-mediated immunity compared to the skin.
LABORATORY INVESTIGATION
(2024)