期刊
JOURNAL OF PROTEOME RESEARCH
卷 19, 期 5, 页码 1923-1940出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.9b00752
关键词
redox proteomics; retinoic acid; neuronal differentiation; SH-SYSY cells; H2O2 signaling
资金
- research commission (FOKO) of the medical faculty, Heinrich-Heine-Universitat Dusseldorf
The initial phases of neuronal differentiation are key to neuronal function. A particularly informative model to study these initial phases are retinoic acid-stimulated SH-SYSY cells. Although these progressions are associated with redox-sensitive processes, it is largely undefined how the cellular proteome underpins redox dynamics and the management of reactive oxygen species. Here, we map the global cysteine-based redox landscape of SH-SYSY cells using quantitative redox proteomics. We find evidence that redox alterations occurred early in differentiation and affect the expression of neuronal marker proteins and the extension of neurites. The spatiotemporal analysis of reactive oxygen species suggests a NOX2-dependent peak in cytoplasmic superoxide anions/hydrogen peroxide generation 2 h after retinoic acid stimulation. At the same time point, 241 out of 275 proteins with an altered cysteine redox state are reversibly oxidized in response to retinoic acid. Our analyses pinpoint redox alterations of proteins involved in the retinoic acid homeostasis and cytoskeletal dynamics.
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