Article
Cardiac & Cardiovascular Systems
Jingyuan Hou, Qiaoting Deng, Sudong Liu, Xiaohong Qiu, Xunwei Deng, Wei Zhong, Zhixiong Zhong
Summary: This study investigated the differences in plasma protein profiles between patients with in-stent restenosis (ISR), non-ISR patients, and healthy controls. The findings revealed several differentially abundant proteins that are involved in important biological processes and signaling pathways related to ISR. The identified proteins, including fetuin-B, apolipoprotein C-III (APOC3), and cholesteryl ester transfer protein (CETP), could potentially serve as early diagnostic biomarkers and therapeutic targets for ISR.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemical Research Methods
Ting Huang, Mateusz Staniak, Felipe da Veiga Leprevost, Amanda M. Figueroa-Navedo, Alexander R. Ivanov, Alexey I. Nesvizhskii, Meena Choi, Olga Vitek
Summary: Repeated measures experimental designs are commonly used in mass spectrometry-based proteomics to quantify proteins in biological subjects repeatedly over multiple conditions or times. Combining repeated measures with tandem mass tag (TMT) labeling presents statistical challenges due to unique interplays of variation. This manuscript proposes a family of linear mixed-effects models for analyzing proteomics experiments with repeated measures and TMT multiplexing, which decompose the variation and enable statistical inference of differential protein abundance.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Immunology
Haoran Guo, Qi Wang, Tao Li, Jingwen Chen, Chao Zhang, Ying Xu, Qing Chang, Hangyi Li, Weiqiang Sun, Ruidi Han, Chi Wang, Chengbin Wang
Summary: This study discovered that ascending to high altitude can lead to physiological and molecular changes, resulting in some individuals being unable to acclimatize. Predicting acute mountain sickness (AMS) prior to ascent remains a challenge. Proteomic analysis revealed significant differences in inflammatory and immune-related processes between AMS-susceptible and AMS-resistant groups. Potential biomarkers, such as SAP, AAT, LTF, and HSP90-alpha, were identified for the prediction of AMS at low altitudes.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Abhilash Barpanda, Ankit Halder, Ayushi Dhote, Shashwati Parihari, Chetan Kantharia, Sanjeeva Srivastava
Summary: Colorectal cancer is the second leading cause of cancer death globally. By using systems science and quantitative proteomics, researchers have identified 22 significantly altered protein candidates associated with the growth, survival, and metastasis of the tumor. Additionally, biological pathway analysis revealed dysregulation in key biological processes, including purine metabolism, MYC targets in cancer, DNA repair and replication, and leukocyte transendothelial migration. These findings have important implications for understanding the pathobiology, diagnostics, and therapeutics development in colorectal cancer.
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
(2023)
Article
Engineering, Environmental
Leonard Barnabas Ebinezer, Ilaria Battisti, Nisha Sharma, Laura Ravazzolo, Lokesh Ravi, Anna Rita Trentin, Giuseppe Barion, Anna Panozzo, Stefano Dall'Acqua, Teofilo Vamerali, Silvia Quaggiotti, Giorgio Arrigoni, Antonio Masi
Summary: This study investigated the bioaccumulation and effects of PFAS on the growth, physiology, and root proteome of hydroponically-grown maize plants treated with a combination of eleven different PFAS. The results showed that PFAS exposure inhibited root growth, decreased the fresh weight of leaves and roots, and altered photosynthetic parameters. Short-chain PFAS were detected in higher concentrations in the leaves, while long-chain PFAS were mostly retained in the roots. Proteome analysis revealed differential abundance of proteins involved in cellular metabolic and biosynthetic processes, translation, and cytoskeletal reorganization. These findings provide valuable insights into the metabolic changes of plants exposed to PFAS from a proteomics perspective.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Veterinary Sciences
Pagona G. Gouletsou, George Th. Tsangaris, Eleni I. Katsarou, Maria V. Bourganou, Mariana S. Barbagianni, Athina P. Venianaki, Efterpi Bouroutzika, Athanasios K. Anagnostopoulos, George C. Fthenakis, Angeliki I. Katsafadou
Summary: Proteomics is used to study the proteins present in a sample and their expression under different conditions. This study used high-throughput technologies to analyze the proteome of dog semen and identified proteins related to sperm maturation and motility. Future research can focus on applying this technique to animals with suboptimal fertility.
VETERINARY SCIENCES
(2022)
Article
Cell Biology
Claudio de Lucia, Michela Piedepalumbo, Lu Wang, Fausto Carnevale Neto, Daniel Raftery, Erhe Gao, Domenico Pratico, Daniel E. L. Promislow, Walter J. Koch
Summary: This study aimed to evaluate the effects of aging and ischemia/reperfusion injury on plasma metabolomic profiling in mice. The results showed that aging significantly affects circulating metabolite levels, while ischemic injury did not have a drastic impact on LV function in aged mice compared to young mice.
Article
Food Science & Technology
Jing Pu, Bingye Zhao, Xin Liu, Shugang Li, Beibei Wang, Di Wu, Jinqiu Wang, Fang Geng
Summary: This study comprehensively compared the protein profiles and properties of chicken egg white and its three components. The proteomes of thick and thin egg white are similar, but thick egg white has higher abundance of ovomucin subunits and lysozymes. The properties of thick and thin egg white are significantly different. Additionally, the chalaza component has higher abundance of insoluble proteins and lower abundance of soluble proteins, which explains its insolubility. These findings are important for further research and development of egg white, such as thinning of egg white, understanding changes in egg white properties, and differential application of thick and thin egg white.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Yixin Hu, Jianyuan Wu, Xin Jiang, Guiying Chen, Yang Zhang, Luqin Si, Hongliang Jiang, Jiangeng Huang, Jianying Huang
Summary: A rapid, simple and sensitive LC-MS/MS method was developed and validated to quantify abiraterone and its metabolites in human plasma. The method showed good accuracy, precision and stability, making it suitable for pharmacokinetic studies.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Dilip Kumar Singh, Abdul Basit, Allan E. Rettie, Nathan Alade, Kenneth Thummel, Bhagwat Prasad
Summary: Gamma carboxylation is a crucial post-translational modification involved in maintaining biological stability. This study utilizes liquid chromatography-mass spectrometry with an alkaline mobile phase and polarity switching technique to simultaneously identify and quantify gamma-carboxylated proteins. This method provides insights into the active state of VKDPs.
ANALYTICA CHIMICA ACTA
(2023)
Article
Geriatrics & Gerontology
Yuko Yamaguchi, Marta Zampino, Toshiko Tanaka, Stefania Bandinelli, Yusuke Osawa, Luigi Ferrucci, Richard D. Semba
Summary: The study found that circulating GDF-15 is associated with the development of anemia in older adults, and higher levels of GDF-15 are linked to an increased risk of anemia.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2021)
Article
Parasitology
Yangrui Luo, Dun Liu, Yuanmei Wang, Fan Zhang, Yankun Xu, Qian Pu, Lu Zhao, Tianqi Wei, Ting Fan, Yuqi Lou, Shiping Liu
Summary: This study investigates the differential accumulation of proteins and metabolites in miR-1174-depleted Ae. aegypti mosquitoes using proteomic and metabolomic techniques. It identifies the upregulated gene PNP as playing a key role in gut physiological activities.
PARASITES & VECTORS
(2023)
Article
Biochemistry & Molecular Biology
Sumaiya Nazli, Kip D. Zimmerman, Angelica M. Riojas, Laura A. Cox, Michael Olivier
Summary: The proteomic analysis of plasma has great potential for precision medicine and biomarker identification. However, detecting and quantifying low abundance proteins in plasma is challenging. This study developed a mass spectrometry approach using tandem mass tags (TMT) and a tissue reference sample, which successfully identified and quantified numerous proteins in nonhuman primate plasma samples. The methodology promises to increase the number of putative disease biomarkers by efficiently quantifying plasma proteins released from specific tissues.
Article
Biochemical Research Methods
Vincent R. Richard, Claudia Gaither, Robert Popp, Daria Chaplygina, Alexander Brzhozovskiy, Alexey Kononikhin, Yassene Mohammed, Rene P. Zahedi, Evgeny N. Nikolaev, Christoph H. Borchers
Summary: This study used multiplexed targeted mass spectrometry assays to determine the concentrations of hundreds of substances in the plasma of hospitalized COVID-19 patients. The researchers found clear distinctions between COVID-19 patients and controls, as well as significant differences between survivors and nonsurvivors. Using a machine learning model, they were able to predict patient survival with high accuracy based on the concentrations of a limited number of substances on the day of hospitalization.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Biochemistry & Molecular Biology
Heba Badr, Ron Blutrich, Kaitlin Chan, Jiefei Tong, Paul Taylor, Wen Zhang, Ran Kafri, Hannes L. Rost, Ming-Sound Tsao, Michael F. Moran
Summary: Proteome analysis revealed co-expressed upregulated metabolism proteins highly conserved between primary NSCLC and patient-derived xenograft tumors. The encoded genes of these proteins are associated with the survival of NSCLC patients, suggesting their role in driving NSCLC through metabolic processes.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Yan-Jing Liang, Yuan-Rui Yang, Chuan-Yuan Tao, Su-Hao Yang, Xin-Xiao Zhang, Jing Yuan, Yuan-Hong Deng, Zhan-Qiong Zhong, Shu-Guang Yu, Xiao-Yi Xiong
Summary: The study revealed that TLR4 signaling pathways affect protein succinylation processes in mouse ICH brains, with TAK242 treatment leading to both increased and decreased succinyllysine sites in the brain. These changes were mainly located in the mitochondria and cytoplasm of the cells.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Article
Neuroimaging
Johannes Kaesmacher, Adnan Mujanovic, Kilian Treurniet, Manon Kappelhof, Thomas R. Meinel, Pengfei Yang, Jianmin Liu, Yongwei Zhang, Wenjie Zi, Qingwu Yang, Raul G. Nogueira, Kazumi Kimura, Yuji Matsumaru, Kentaro Suzuki, Bernard Yan, Peter J. Mitchell, Zhongrong Miao, Yvo B. W. E. M. Roos, Charles B. L. M. Majoie, Jan Gralla, Jeffrey L. Saver, Urs Fischer
Summary: This study investigated the acceptable level of uncertainty for experienced stroke clinicians in deciding whether to administer intravenous thrombolysis (IVT) before endovascular treatment (EVT). The results showed that clinicians would generally feel comfortable skipping IVT if there was reasonable comparability between IVT+EVT and EVT alone, with no more than 3% of patients being unable to achieve functional independence at 90 days due to skipping IVT.
JOURNAL OF NEUROINTERVENTIONAL SURGERY
(2023)
Review
Endocrinology & Metabolism
Xiao-Yi Xiong, Yong Tang, Qing-Wu Yang
Summary: This review discusses the function of reactive astrocytes under pathological conditions and summarizes the emerging roles of glucose and lipid metabolism in reactive astrocytes in the context of CNS injury, offering new insights into the metabolic mechanisms of neuroprotection or damage mediated by reactive astrocytes.
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2022)
Article
Neurosciences
Changxiong Gong, Jiacheng Huang, Weilin Kong, Fengli Li, Chang Liu, Jie Yang, Shuai Liu, Zhongming Qiu, Min Lin, Zhangbao Guo, Zhizhong Yan, Xianjun Huang, Shuai Zhang, Wentong Ling, Peiyang Zhou, Zhen Wang, Yong Liu, Dongzhang Xue, Yaoyi Zhong, Shu Yang, Yue Wan, Jiayang Fang, Wenguo Huang, Huihui Liu, Jun Luo, Rongzhong Li, Changming Wen, Xinmin Fu, Mingyi Tu, Li Wang, Xiguang Tian, Huiyuan Peng, Zhilin Wu, Guoyong Zeng, Wenjie Zi, Qingwu Yang
Summary: This study found that patients with acute LVO who underwent EVT had sustained beneficial outcomes and functional recovery rates at least 5 years later. Younger age, lower functional disability at 90 days, and absence of stroke recurrence were significantly associated with favorable outcomes at 5 years. Advanced age, higher functional disability at 90 days, and atrial fibrillation were independent factors for stroke recurrence.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Clinical Neurology
Chenhao Zhao, Weidong Luo, Xing Liu, Jun Luo, Jiaxing Song, Junjie Yuan, Shuai Liu, Jiacheng Huang, Weilin Kong, Jinrong Hu, Jie Yang, Ruidi Sun, Chengsong Yue, Dongjing Xie, Linyu Li, Hongfei Sang, Zhongming Qiu, Fengli Li, Deping Wu, Wenjie Zi, Qingwu Yang
Summary: Comorbid atrial fibrillation does not significantly affect the safety and efficacy of mechanical thrombectomy for acute basilar artery occlusion. However, patients with comorbid atrial fibrillation have a higher proportion of ischemic recurrence within one year after treatment.
FRONTIERS IN NEUROLOGY
(2022)
Article
Clinical Neurology
Jiacheng Huang, Maolin Wang, Fengli Li, Weilin Kong, Da Liu, Huagang Li, Peiyang Zhou, Zhizhong Yan, Yan Wang, Jiaxing Song, Weidong Luo, Shuai Liu, Linyu Li, Jinrong Hu, Yan Tian, Zhongming Qiu, Rui Xu, Wouter J. Schonewille, Wenjie Zi, Qingwu Yang
Summary: This study identified potential modifiable risk factors for early mortality in patients with basilar artery occlusion, including recanalization failure, herniation, gastrointestinal hemorrhage, and pneumonia.
CEREBROVASCULAR DISEASES
(2023)
Article
Clinical Neurology
Wenjie Zi, Jiaxing Song, Zhongming Qiu, Weilin Kong, Jiacheng Huang, Weidong Luo, Shuai Liu, Hongfei Sang, Jie Yang, Linyu Li, Yan Tian, Jinrong Hu, Jeffrey L. Saver, Raul G. Nogueira, Fengli Li, Qingwu Yang
Summary: This study aims to compare the efficacy and safety of tirofiban with aspirin in patients with acute ischemic stroke. The study will be conducted in multiple stroke centers in China and will evaluate the functional outcomes and potential safety issues in patients after 90 days of treatment.
INTERNATIONAL JOURNAL OF STROKE
(2023)
Article
Immunology
Lexing Xie, Shuang Zhang, Li Huang, Zhouzhou Peng, Hui Lu, Qian He, Ru Chen, Linlin Hu, Bingqiao Wang, Baoliang Sun, Qingwu Yang, Qi Xie
Summary: Stroke is a leading cause of death and disability worldwide. Researchers have found that peripheral immune cells infiltrate the brain and cause inflammation after cerebral ischemia. However, it is still unclear how and when these immune cells affect the central inflammatory response, and whether interventions targeting these processes can reduce ischemia-reperfusion injury.
BRAIN BEHAVIOR AND IMMUNITY
(2023)
Article
Pharmacology & Pharmacy
Qiong Chen, Ting Zhou, Jun-jie Yuan, Xiao-yi Xiong, Xue-hui Liu, Zong-ming Qiu, Lin-lin Hu, Hui Lu, Qian He, Chang Liu, Qing-wu Yang
Summary: This study investigated the metabolic alterations during ischemia-reperfusion injury using metabolomic analysis. A total of 82 differentially expressed metabolites were identified, with some significantly elevated in the reperfusion group. Metabolomics pathway analysis revealed the importance of glycerophospholipid metabolism, linoleic acid metabolism, pyrimidine metabolism, and galactose metabolism in the response to cerebral ischemia-reperfusion. This study provides important insights into the metabolic changes induced by cerebral ischemia-reperfusion.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Neurosciences
Zhouzhou Peng, Jiaxing Song, Linyu Li, Changwei Guo, Jie Yang, Weilin Kong, Jiacheng Huang, Jinrong Hu, Shuai Liu, Yan Tian, Dahong Yang, Fengli Li, Wenjie Zi, Dongjing Xie, Qingwu Yang
Summary: This study aimed to evaluate the association between stress hyperglycemia ratio (SHR) and clinical outcomes at 90 days in acute ischemic stroke due to large vessel occlusion receiving endovascular treatment. The study found that SHR was negatively associated with the odds of achieving favorable functional outcomes at 90 days.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Review
Medicine, General & Internal
Zhiyi Chen, Xuerong Liu, Qingwu Yang, Yan-Jiang Wang, Kuan Miao, Zheng Gong, Yang Yu, Artemiy Leonov, Chunlei Liu, Zhengzhi Feng, Hu Chuan-Peng
Summary: This study systematically evaluated the clinical applicability and reporting quality of neuroimaging-based AI models for psychiatric diagnosis. The results showed high risk of bias and poor reporting quality. The application of these models in clinical practice still faces challenges and needs to address the risk of bias and improve reporting quality.
Article
Clinical Neurology
Chenhao Zhao, Ting Hu, Weilin Kong, De Yang, Junfang Wan, Kefeng Lv, Jiasheng Liao, Zhao Chen, He Jiang, Deping Wu, Ping Yang, Wenjie Zi, Fengli Li, Qingwu Yang
Summary: This study aimed to evaluate the impact of FPE on efficacy and safety for patients with acute basilar artery occlusion (BAO). The results showed that achieving FPE was strongly associated with favorable clinical outcomes, lower mortality rate, and shorter time to recanalization. Compared with final successful reperfusion, FPE also independently contributed to favorable outcomes.
JOURNAL OF NEUROSURGERY
(2023)
Article
Clinical Neurology
Jie Yang, Fengli Li, Zhongming Qiu, Yan Wang, Jun Luo, Youlin Wu, Guoyong Zeng, Yue Wan, Shuai Liu, Chengsong Yue, Jiaxing Song, Weidong Luo, Chang Liu, Linyu Li, Ruidi Sun, Jiacheng Huang, Hongfei Sang, Chenhao Zhao, Qingwu Yang, Wenjie Zi
Summary: The study aimed to investigate the efficacy and safety of combining endovascular treatment (EVT) with standard medical treatment (SMT) in Chinese patients with acute basilar artery occlusion (BAO) within 6 hours of occlusion. Patients were divided into SMT-alone group or EVT+SMT group. Results showed that EVT was associated with a higher rate of favorable functional outcome and a lower proportion of deaths. Predictors of favorable outcome in the EVT+SMT group included younger age, lower baseline National Institutes of Health Stroke Scale score, higher baseline posterior circulation Alberta Stroke Program Early CT Score, absence of diabetes mellitus, and modified Thrombolysis in Cerebral Infarction scores 2b-3.
JOURNAL OF NEUROSURGERY
(2023)
Article
Clinical Neurology
Jiacheng Huang, Weilin Kong, Zhongming Qiu, Da Liu, Huagang Li, Peiyang Zhou, Zhizhong Yan, Yan Wang, Weipeng Dai, Chong Zheng, Rongzong Li, Jian Wang, Jiaxing Song, Junjie Yuan, Weidong Luo, Shuai Liu, Linyu Li, Hongfei Sang, Fengli Li, Deping Wu, Qingwu Yang, Wenjie Zi
Summary: Authors of this study aimed to investigate the efficacy and safety of endovascular treatment (EVT) versus standard medical treatment (SMT) alone in patients with acute basilar artery occlusion (BAO) and moderate deficit (National Institutes of Health Stroke Scale [NIHSS] score 10-19). The results showed that EVT leads to improved outcomes compared to those with SMT alone, and younger age, absence of diabetes mellitus, higher baseline scores, and better reperfusion are associated with better functional outcome in the EVT group.
JOURNAL OF NEUROSURGERY
(2022)
