期刊
JOURNAL OF NEUROSCIENCE
卷 40, 期 27, 页码 5327-5340出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1670-19.2020
关键词
CA1; dendrite; FMRP; HCN; I-h; PFC
资金
- National Institutes of Health [R01 MH100510, R01 MH094839]
- Swiss National Science Foundation [P2ZHP3_168621, P408PB_188785]
- McKnight Foundation
- Swiss National Science Foundation (SNF) [P2ZHP3_168621] Funding Source: Swiss National Science Foundation (SNF)
Channelopathies are implicated in Fragile X syndrome (FXS), yet the dysfunction of a particular ion channel varies with cell type. We previously showed that HCN channel function is elevated in CAI dendrites of the fmr1(-/gamma) mouse model of FXS, but reduced in L5 PFC dendrites. Using male mice, we tested whether Fragile X Mental Retardation Protein (FMRPO), the protein whose absence causes FXS, differentially modulates HCN channels in CAI versus L5 PFC dendrites. Using a combination of viral tools, intracellular peptide, and dendritic electrophysiology, we found that FMRP regulates HCN channels via a cell-autonomous protein protein interaction. Virally expressed FMRP restored WT HCN channel-related dendritic properties in both CAI and 15 neurons. Rapid intracellular perfusion of the non-mRNA binding N-terminal fragment, FmRP1,298, similarly restored dendritic function. In support of a protein-protein interaction, we found that FMRP associated with HCN-TRIP8b complexes in both hippocampus and PFC. Finally, voltage-clamp recordings showed that FMRP modulated I-h byd regulating the number of functional dendritic HCN channels rather than individual channel properties. Together, these represent three novel findings as to the nature of the changes in dendritic function in CAI and PFC neurons based on the presence or absence of FMRP. Moreover, our findings provide evidence that FMRP can regulate its targets in opposite directions depending upon the cellular milieu.
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