期刊
JOURNAL OF MOLECULAR HISTOLOGY
卷 51, 期 3, 页码 317-327出版社
SPRINGER
DOI: 10.1007/s10735-020-09882-9
关键词
CircRNA; Circular RNA; CircHIPK3; miR-637; Clear cell renal carcinoma; Renal carcinoma
类别
资金
- National Key Research and Development Program of China [2016YFC1305703]
- Medical Scientific Research Foundation of Guangdong Province, China [A2019571]
- Fundamental Research Funds for the Central Universities [21618306, 21618303]
- Science and technology project of Yangjiang city [33518008]
- Leading Specialist Construction Project-Department of Urology
- First Affiliated Hospital, Jinan University [711006]
Increasing evidence has indicated that circular RNAs (circRNAs) play a key role in the development and progression of diverse cancers, but their role in clear cell renal carcinoma (CCRCC) tumorigenesis is not well understood. In this study, we firstly performed comprehensive circRNA-seq from CCRCC tissues and pair-matched adjacent normal tissues. In total, 1184 circRNAs were dysregulated in human CCRCC tissues compared with those in adjacent normal tissues. We randomly selected four circRNAs, including circHIPK3 (circBase ID: hsa_circ_0000284), to test the circRNA-seq data in another 40 CCRCC tissues by quantitative real-time PCR (qRT-PCR). Furthermore, we found that circHIPK3 was downregulated in CCRCC tissues and cell lines. Overexpression of circHIPK3 effectively suppressed CCRCC cell invasion and migration in vitro, and inhibited CCRCC cell proliferation in vitro and in vivo. Moreover, bioinformatic analysis and luciferase reporter assay showed that circHIPK3 targeted miR-637 in CCRCC cells. Hence, CircHIPK3 may represent a tumor suppressor and target miR-637 in clear cell renal carcinoma.
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