Article
Engineering, Biomedical
Ziyuan Chen, Jintao Shen, Meng Wei, Wenrui Yan, Qiucheng Yan, Zhangyu Li, Yaqiong Chen, Feng Zhang, Lina Du, Bochuan Yuan, Yiguang Jin
Summary: Nuclear safety is globally concerning due to the harm caused by ionizing radiation to organisms. This study developed a living composite hydrogel consisting of engineered bacteria and gelatin/sodium alginate for the biological detection of ionizing radiation. The hydrogel showed significant green fluorescence under gamma-ray radiation, providing a biological strategy for environmental radiation detection.
BIO-DESIGN AND MANUFACTURING
(2023)
Article
Biotechnology & Applied Microbiology
Farnaz Ghorbani, Minjoo Kim, Mahshid Monavari, Behafarid Ghalandari, Aldo R. Boccaccini
Summary: This study demonstrated the fabrication of PDA-coated ADA-GEL scaffolds using extrusion-based 3D printing technology for bone regeneration. The PDA layer contributed to enhanced mechanical stability and hydrophilicity, as well as satisfactory biomineralization. The scaffolds showed potential for promoting osteosarcoma cell adhesion, viability, and proliferation, and could potentially induce bone regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Aydin Bordbar-Khiabani, Ilijana Kovrlija, Janis Locs, Dagnija Loca, Michael Gasik
Summary: In this study, alginate hydrogels with and without octacalcium phosphate (OCP) were fabricated on 3D-printed titanium alloys to improve their corrosion resistance in various simulated environments. The results showed that the OCP-laden alginate hydrogel exhibited higher hydrophilicity and increased crystallinity compared to pure alginate. Additionally, the OCP particles significantly enhanced the corrosion resistance of the coated samples. Therefore, this study is of great importance for improving the corrosion resistance of metallic implants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
E. Baigorria, S. Souza dos Santos, M. R. de Moura, L. F. Fraceto
Summary: In this study, 3D printing of bioinks was used to develop nanocomposite hybrid adsorbent hydrogels. By adding clay to the biopolymer matrix, the resulting hydrogels showed improved rheological, mechanical, thermal, and porous properties. The SA-B30 bioink and corresponding 3D-printed device were found to be the best systems for adsorption of the pesticide paraquat. The synthesized eco-friendly, biodegradable, and low-cost materials can contribute to a circular economy and ensuring safe water for human consumption.
MATERIALS TODAY CHEMISTRY
(2023)
Article
Chemistry, Applied
Aurora C. Hernandez-Gonzalez, Lucia Tellez-Jurado, Luis M. Rodriguez-Lorenzo
Summary: Organic-inorganic hybrid materials were prepared by covalently coupling silica and alginate through Schiff base formation and sol-gel reaction. The study found that a higher ADA content favored the sol-gel reaction, while introducing ionic crosslinking treatment increased the modulus of the hybrids. The proposed method successfully synthesized ADA-Silica hybrid hydrogels with both ionic and covalent crosslinking.
CARBOHYDRATE POLYMERS
(2021)
Article
Engineering, Biomedical
Abudureheman Maihemuti, Han Zhang, Xiang Lin, Yangyufan Wang, Zhihong Xu, Dagan Zhang, Qing Jiang
Summary: In this study, a three-dimensional printed porous multilayer scaffold made of cold-water fish skin gelatin was developed for osteoarticular cartilage regeneration. The scaffold mimics the structure of native cartilage, allowing chondrocytes to adhere, proliferate, and communicate, as well as transport nutrients and prevent further joint damage. Moreover, the gelatin scaffolds were found to be nonimmunogenic, nontoxic, and biodegradable. Implanting the scaffold into rat cartilage defects resulted in satisfactory repair outcomes in an animal model. Therefore, cold-water fish skin gelatin scaffolds have promising applications in regenerative medicine.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Tyler Gregory, Prateek Benhal, Annie Scutte, David Quashie Jr, Kiram Harrison, Casey Cargill, Saliya Grandison, Mary Jean Savitsky, Subramanian Ramakrishnan, Jamel Ali
Summary: Increasing cell seeding densities have shown an inverse relationship with shear viscosity and a reduction in storage moduli, leading to lowered printing pressures for gel extrusion. However, higher cell concentrations may negatively impact the structural properties of the extruded material.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Materials Science, Biomaterials
T. Distler, L. Kretzschmar, D. Schneidereit, S. Girardo, R. Goswami, O. Friedrich, R. Detsch, J. Guck, A. R. Boccaccini, S. Budday
Summary: This study investigates the properties of hydrogels containing cells and microgel beads through complex mechanical analyses. The results show that the stiffness of hydrogels significantly decreases when the cell and bead concentrations exceed certain values. Hydrogels with higher cell concentrations exhibit more pronounced material nonlinearity for larger strains and faster stress relaxation.
BIOMATERIALS SCIENCE
(2021)
Article
Polymer Science
Arlina Prima Putri, Ranjita K. Bose, Mochamad Chalid, Francesco Picchioni
Summary: Alginate dialdehyde and l-lysine-functionalized alginate dialdehyde were synthesized and characterized. The NMR analysis confirmed the introduction of l-lysine functionality, and FTIR spectroscopy revealed the formation of Schiff base covalent linkage after crosslinking. The hydrogels with l-lysine-functionalized alginate dialdehyde exhibited higher modulus storage rate and showed shear-thinning and self-healing properties. The concentration and stoichiometric ratio of alginate dialdehyde, l-lysine-functionalized alginate dialdehyde, and gelatin played a fundamental role in the mechanical properties of the hydrogel.
Article
Engineering, Biomedical
Thomas Distler, Christian Polley, Fukun Shi, Dominik Schneidereit, Mark. D. Ashton, Oliver Friedrich, Jurgen F. Kolb, John G. Hardy, Rainer Detsch, Hermann Seitz, Aldo R. Boccaccini
Summary: Electroactive hydrogels incorporating pyrrole and high gelatin-content oxidized alginate-gelatin (ADA-GEL) have been developed, with electroactive polypyrrole synthesized inside the ADA-GEL matrix. These hydrogels exhibit 3D printability, cytocompatibility, and conductivity, making them promising for cell therapies and electrical-stimulation assisted tissue engineering.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Jalaja Aswathy, Rajalekshmi Resmi, Josna Joseph, Roy Joseph, Annie John, Annie Abraham
Summary: The study demonstrates that the incorporation of C. gigantea extract (CGE) with self-cross-linked alginate dialdehyde-gelatin (CGEADAG) hydrogel can be used for cartilage tissue regeneration. CGE is rich in bioactive compounds with antioxidant and antibacterial activities. The properties of CGEADAG hydrogel, such as gelation time, water uptake, and pore size, were characterized. Cell proliferation assay on primary chondrocytes showed the regenerative potential of CGE and CGEADAG. This research is important for the treatment of degenerative diseases like osteoarthritis.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hui Wang, Jiaxin Zhang, He Liu, Zhenguo Wang, Guiwei Li, Qingping Liu, Chenyu Wang
Summary: The study found that PU material treated with ultrasonication and high temperature showed good similarity in simulating the mechanical properties of auricular cartilage. The PU scaffold filled with chondrocytes successfully integrated with normal auricular cartilage, indicating its potential for clinical repair of ear cartilage damage.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Resmi Rajalekshmi, Anusree Kaladevi Shaji, Roy Joseph, Anugya Bhatt
Summary: The development of a tissue-engineered construct for hepatic regeneration is challenging due to the lack of an optimum environment for hepatocyte growth. Injectable alginate dialdehyde-gelatin hydrogel incorporating fibrin was explored as a matrix for liver tissue engineering, showing promising support for the growth and function of HepG2 cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Biomedical
Stephen W. Sawyer, Kazuyo Takeda, Alaadin Alayoubi, Eman Mirdamadi, Ahmed Zidan, Steven R. Bauer, Heba Degheidy
Summary: 3D bioprinting technology has great potential in the fields of regenerative medicine and tissue engineering. This study optimized the bioprinting protocol to successfully print constructs containing hMSCs, demonstrating their stability and bioactivity.
BIOMEDICAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Minghao Zhang, Tianbao Qian, Ziwei Deng, Fei Hang
Summary: The developed DN hydrogel with improved mechanical properties and bioactivity due to the synergy of covalent and ionic cross-linking networks is highly promising for complex tissue engineering scaffolds. The inclusion of PolyP enhances the bioenergetic effect, promoting cell activities and accelerating osteogenic differentiation, making it a potential solution for bone tissue regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Biomedical
Aylin Kara Ozenler, Thomas Distler, Funda Tihminlioglu, Aldo R. Boccaccini
Summary: The development of biomaterial inks mimicking the physicochemical properties of the extracellular matrix is crucial for the application of bioprinting in tissue engineering. In this study, a composite biomaterial ink composed of fish scale gelatin and alginate dialdehyde was developed for 3D bioprinting applications, which showed improved mechanical strength and osteogenic differentiation of MC3T3-E1 cells. These findings suggest that fish scale gelatin can be a promising additive in bone tissue engineering applications.
Article
Materials Science, Multidisciplinary
Hsuan-Heng Lu, Kai Zheng, Aldo R. Boccaccini, Liliana Liverani
Summary: Ce-doped calcium silicate-based bioactive glass sols were electrospun and heat-treated to form cotton-like fibrous structures. The 3D structures consisted of long and entangled fibers with interconnected pores, and the successful incorporation of Ce was confirmed by EDX analysis. The fibers exhibited high hydrophilicity and in vitro bioactivity in simulated body fluid. They showed no cytotoxicity to keratinocytes and did not hinder the wound closure process in vitro.
Article
Materials Science, Multidisciplinary
Lukasz Pawlowski, Muhammad Asim Akhtar, Andrzej Zielinski, Aldo R. Boccaccini
Summary: The aim of this study was to investigate the response of coatings composed of chitosan (CS), Eudragit E 100 (EE100), or poly(4-vinylpyridine) (P4VP) on a silver nanoparticle (AgNPs)-decorated titanium substrate when in contact with human osteoblast-like MG-63 cells. The biopolymer coatings were successfully deposited on the substrate with high uniformity, and the deposited silver did not exhibit cytotoxicity. The biological study showed that the CS/EE100 coating performed better than the CS/P4VP coating, with a decrease in MG-63 cell viability observed only after three days of testing.
Article
Pharmacology & Pharmacy
Mirna L. Sanchez, Hugo Valdez, Micaela Conde, Pamela Viana-Mendieta, Aldo R. Boccaccini
Summary: This article discusses the development of biomaterial platforms using functional hydrogels for delivering reagents of interest in the field of biomedicine. In situ dosing of therapeutic components for dermatological injuries, such as diabetic foot ulcers, is a novel strategy to improve wound healing. Hydrogels have shown advantages over other therapies in terms of comfort and their compatibility with tissues. The paper also proposes a new approach to wound care by modulating macrophage polarization using advanced biomaterials.
Editorial Material
Biotechnology & Applied Microbiology
Farnaz Ghorbani, Behafarid Ghalandari, Aldo R. Boccaccini, Derek H. Rosenzweig
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Thomas Gruhn, Camilo Ortiz Monsalve, Claudia Mueller, Susanne Heid, Aldo R. Boccaccini, Sahar Salehi
Summary: In this study, the dynamics of anisotropic fillers in fibrous structures with hydrogel-based matrices were analyzed using computational simulations. Microfabricated rods were used as anisotropic fillers in the fabrication of composite filaments and their orientation in the fibers was evaluated experimentally. The results showed that the fillers were not well aligned during the extrusion process, leading to a random distribution in the fibers.
BIOENGINEERING-BASEL
(2023)
Review
Engineering, Biomedical
Lisa Schoebel, Aldo R. Boccaccini
Summary: This review comprehensively summarizes recent research on glycosaminoglycan-modified electrically conductive polymers for biomedical applications. It discusses the effect of different biological dopants on material characteristics and summarizes the key findings of biological characterization in vitro and in vivo. The remaining challenges in the field are also discussed.
ACTA BIOMATERIALIA
(2023)
Article
Materials Science, Multidisciplinary
Slila Chayanun, Anahita Ahmadi Soufivand, Jessica Faber, Silvia Budday, Boonrat Lohwongwatana, Aldo R. Boccaccini
Summary: Cartilage tissue engineering is improved by adding nanofibrillated cellulose (NFC) to alginate dialdehyde-gelatin (ADA-GEL) hydrogel, resulting in increased stiffness of the composite hydrogel. The maximum compressive effective modulus is 19.6 kPa. Cell viability, proliferation, and chondrogenic differentiation of ATDC5 cells are evaluated using immunohistochemical staining. The potential application of an osteochondral plug with polyetheretherketone as the subchondral bone anchor part is proposed.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Electrochemistry
F. Maciag, T. Moskalewicz, K. Cholewa-Kowalska, Z. Hadzhieva, M. Dziadek, B. Dubiel, A. Lukaszczyk, A. R. Boccaccini
Summary: Cu- and Mg-doped mesoporous sol-gel bioactive glasses were developed and their microstructure was investigated. The electrophoretic deposition conditions for composite coatings on Ti-13Nb-13Zr substrates were determined. The coated alloy showed enhanced resistance to electrochemical corrosion and improved antibacterial properties compared to pure zein.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Irem Unalan, Ilenia Occhipinti, Marta Miola, Enrica Verne, Aldo R. Boccaccini
Summary: This study used polycaprolactone (PCL) and melt electrowriting (MEW) technique to fabricate scaffolds coated with superparamagnetic iron oxide nanoparticles (SPIONs) and silica-coated-SPIONs (Si-SPIONs). The hydrophilicity of the scaffolds was improved by alkaline treatment. The scaffolds showed good biocompatibility. The alkaline-treated scaffolds and SPIONs/Si-SPIONs-coated scaffolds exhibited higher pre-osteoblastic cell adhesion and proliferation efficiency. All samples were able to generate heat when subjected to an alternating magnetic field, useful for hyperthermia treatment.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Materials Science, Multidisciplinary
Jonas Hazur, Jonas Roeder, Jonas Czwalinna, Dirk W. W. Schubert, Aldo R. R. Boccaccini
Summary: The main challenge in extrusion-based bioprinting is to develop inks with multiple characteristics before, during, and after printing. Hydrogel microparticles (HMPs) are proposed to produce internally pre-crosslinked inks, which effectively improve printability. Rheological measurements reveal the increase in yield stress and viscosity, as well as the reduction in loss angle, after HMP pre-crosslinking. Additionally, pre-crosslinking significantly alters the hydrogel's internal microstructure and NIH/3T3 cells proliferate in HMP pre-crosslinked bioinks.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Abhishek Indurkar, Rajan Choudhary, Kristaps Rubenis, Mansingraj Nimbalkar, Anatolijs Sarakovskis, Aldo R. Boccaccini, Janis Locs
Summary: Amorphous calcium phosphate (ACP) and its derivatives containing carboxylic ions (ACPC) were synthesized and characterized for their physiochemical properties. A new synthesis approach was developed, and thorough analysis through various techniques revealed differences in the properties of ACP and ACPC. The cytocompatibility of the synthesized ACPs and ACPCs was evaluated, and ACPC synthesized with citrate showed the highest cell viability.
Article
Engineering, Biomedical
Zeqian Xu, Xuanyu Qi, Minyue Bao, Tian Zhou, Junfeng Shi, Zhiyan Xu, Mingliang Zhou, Aldo R. Boccaccini, Kai Zheng, Xinquan Jiang
Summary: Type II diabetes mellitus (TIIDM) poses challenges for dentists and orthopedists, but 3D-printed bioscaffolds containing Sr-MBGNs and GelMA can remodel the diabetic microenvironment, enhance bone regeneration, and promote osteogenic, angiogenic, and immunomodulatory properties through the release of Sr, Ca, and Si ions.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Hazel O. Simila, Ana M. Beltran, Aldo R. Boccaccini
Summary: This study investigated the antimicrobial and mechanical properties of dental floss coated with chitosan or chitosan + mesoporous bioactive glass nanoparticles. The coatings showed significant ability to inhibit bacterial growth after 24 hours, although the impact was minimal at 3 hours. Furthermore, the addition of these layers improved the tensile strength of the floss, making it more effective for cleaning between teeth. Enhanced investigations of these composites are therefore proposed due to their potential in promoting oral health.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2023)
Article
Chemistry, Physical
Soledad Stagnoli, Cintia Garro, Ozlem Ertekin, Susanne Heid, Stefan Seyferth, Gaston Soria, N. Mariano Correa, Aldo Leal-Egana, Aldo R. Boccaccini
Summary: This study developed a system for delivering water-insoluble drugs, which can efficiently load drugs and increase the likelihood of drug uptake by cancer cells instead of healthy cells. The system also demonstrates high efficiency and stability during topical application, and it is less invasive compared to alternative methods.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
