Article
Medical Laboratory Technology
Ximeng Chen, Jianan Wang, Jinyang Wang, Jingyun Ye, Ping Di, Chang Dong, Hong Lei, Chengbin Wang
Summary: This study utilized proteomics to analyze serum proteins and identify potential biomarkers for the diagnosis of osteoarticular tuberculosis. ITIH2, CFHR2, CFHR3, and CFHR5 were found to be potential biomarkers, with CFHR2 and CFHR3 showing good diagnostic efficacy when combined in a diagnostic model.
CLINICA CHIMICA ACTA
(2023)
Article
Medicine, Research & Experimental
Qingbo Shu, Shan Liu, Tonino Alonzi, Sylvia M. LaCourse, Dhiraj Kumar Singh, Duran Bao, Dalton Wamalwa, Li Jiang, Christopher J. Lyon, Grace John-Stewart, Deepak Kaushal, Delia Goletti, Tony Hu
Summary: Using mass spectrometry (MS) to read immunoassays for biomarker-derived peptides can improve sensitivity and specificity, allowing for better identification of tuberculosis cases and differentiation between disease and asymptomatic infection. This approach shows strong potential for infectious disease diagnosis and monitoring changes in Mycobacterium tuberculosis burden for more effective disease control.
Article
Immunology
Ximeng Chen, Jingyun Ye, Hong Lei, Chengbin Wang
Summary: This study utilized liquid chromatography tandem mass spectrometry (LC-MS/MS) to detect and analyze serum metabolites in osteoarticular tuberculosis patients, disease controls, and healthy controls, identifying novel metabolic biomarkers for the diagnosis of osteoarticular tuberculosis. Bioinformatics analysis revealed that these metabolites are involved in lipid metabolic signaling pathways, suggesting the importance of lipid metabolism in the pathological process of tuberculosis. This study provides valuable insights into metabolic biomarkers of osteoarticular tuberculosis and the mechanism of lipid metabolism in tuberculosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Microbiology
Jiubiao Guo, Ximeng Zhang, Xinchun Chen, Yi Cai
Summary: Researchers are utilizing proteomics technology to discover new biomarkers for tuberculosis diagnosis, and while some progress has been made, challenges and uncertainties still remain.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Tuo Liang, Jiarui Chen, GuoYong Xu, Zide Zhang, Jiang Xue, Haopeng Zeng, Jie Jiang, Tianyou Chen, Zhaojie Qin, Hao Li, Zhen Ye, Yunfeng Nie, Chong Liu, Xinli Zhan
Summary: The study developed a new nomogram for the clinical diagnosis of osteoarticular tuberculosis (TB) using xCell score estimation. Analysis revealed differences in xCell scores between TB patients and healthy controls, with higher scores for basophils, monocytes, neutrophils, and platelets in TB patients. The nomogram showed satisfactory performance with an AUC of 0.843. The ML ratio, BMI, and ESR were identified as independent predictive factors for osteoarticular TB diagnosis.
Article
Biochemical Research Methods
Rishabh Sharma, Ajitesh Lunge, Nikita Mangla, Nisheeth Agarwal
Summary: Persisters are a subset of bacteria that resist antibiotics and may contribute to drug-resistant mutations in Mycobacterium tuberculosis. Proteomic analysis reveals that proteins related to various functions such as metabolism, respiration, cell wall, lipid metabolism, information pathways, and virulence are primarily regulated in the persister subpopulation. Surface-localized mycobacterial membrane protein large (MmpL) proteins are crucial for mycobacterial survival during persistent growth state.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Microbiology
Emmanuel C. Ogbonna, Henry R. Anderson, Karl R. Schmitz
Summary: Tuberculosis is a leading cause of infectious mortality worldwide. Research confirms the existence of posttranslational arginine phosphorylation modifications in mycobacteria and other actinobacterial species, providing important insights into protein degradation mechanisms and potential drug targets.
MICROBIOLOGY SPECTRUM
(2022)
Article
Gastroenterology & Hepatology
Josef Ecker, Elisa Benedetti, Alida S. D. Kindt, Marcus Hoering, Markus Perl, Andrea Christel Machmueller, Anna Sichler, Johannes Plagge, Yuting Wang, Sebastian Zeissig, Andrej Shevchenko, Ralph Burkhardt, Jan Krumsiek, Gerhard Liebisch, Klaus-Peter Janssen
Summary: Analysis of the CRC lipidome revealed a robust TG-species signature with prognostic potential, shedding light on the importance of lipid metabolism abnormalities in CRC.
Review
Immunology
Shudi Guo, Ming Tian, Yunping Fan, Xiangyang Zhang
Summary: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a complex and heterogeneous disease diagnosed through endoscopy and computed tomography, and treated with glucocorticoid or surgery. Proteomics and metabolomics have shown potential in elucidating the mechanisms underlying CRSwNP using mass spectrometry as a powerful tool. Recent advances in non-invasive samples, exosomes, and metabolomics research have paved the way for further understanding of CRSwNP.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Liyan Mao, Sylvia M. LaCourse, Soyeon Kim, Chang Liu, Bo Ning, Duran Bao, Jia Fan, Christopher J. Lyon, Ziyong Sun, Sharon Nachman, Charles D. Mitchell, Tony Y. Hu
Summary: This study evaluated the use of serum CFP-10 signal for diagnosing tuberculosis in HIV-infected and HIV-uninfected infants, demonstrating high sensitivity and specificity for TB diagnosis. The serum CFP-10 signal showed potential utility for early TB detection and monitoring of anti-TB treatment responses in children.
Article
Food Science & Technology
Lingping Hu, Hongwei Zhang, Zhiheng Hu, Yaoxian Chin, Gaoshang Li, Jiayin Huang, Xiaomei Zhang, Bingxue Jiang, Yaqin Hu
Summary: This study analyzed the lipid profiles of three commercial tuna species using UPLC-Q-Exactive Orbitrap MS. It identified 27 potential lipid biomarkers that can effectively differentiate different tuna species.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Aron Bartha, Zsuzsanna Darula, Gyoengyi Munkacsy, Eva Klement, Peter Nyirady, Balazs Gyorffy
Summary: This research used a proteotranscriptomic approach to differentiate normal and tumor tissues in clear cell renal cell carcinoma (ccRCC). Top genes over-expressed in ccRCC were identified using transcriptomic data of patients with malignant and normal tissue samples. Protein level analysis and mass spectrometry further validated the differential protein abundance of these genes. A minimal panel of proteins highly specific for ccRCC tissues was identified using transcriptomic and proteomic data.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Tingting Zhang, Xia Yu, Shu'an Wen, Yi Xue, Hua Xiao, Ruyan Ren, Fen Wang, Lingling Dong, Shibing Qin, Hairong Huang
Summary: The study found that cycloserine can effectively penetrate into the bone and achieve concentrations comparable to those in plasma in patients undergoing osteoarticular tuberculosis surgery. This is beneficial for the treatment of osteoarticular tuberculosis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Cosima Damiana Calvano, Ilario Losito, Tommaso Cataldi
Summary: This special issue focuses on the contribution of dysregulation of lipid signaling and metabolism to neurodegenerative diseases, including Alzheimer's and Parkinson's, summarizing three reviews and one research article.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medical Laboratory Technology
Amandine Amalric, Amandine Bastide, Aurore Attina, Armelle Choquet, Jerome Vialaret, Sylvain Lehmann, Alexandre David, Christophe Hirtz
Summary: Despite significant progress in targeted therapies, cancer recurrence remains a major cause of mortality worldwide. Identification of accurate biomarkers through molecular profiling in healthy and cancer patient samples can improve cancer diagnosis and advance personalized medicine.
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2022)