期刊
CHEMICAL BIOLOGY & DRUG DESIGN
卷 89, 期 4, 页码 475-481出版社
WILEY
DOI: 10.1111/cbdd.12883
关键词
benzophenone; detyrosination; Ginkgo biloba; mass spectrometry; alpha-tubulin
资金
- NIMHD/NIH [G12, MD007599-27]
- NIH/NIGMS [R01GM062939, R01GM099481]
Ginkgolides are terpene trilactones in Ginkgo biloba, a popular medicinal herb for memory disorders. Although ginkgolides are known for various neurobiological effects, their macromolecular target in brain is unknown. In this work, we employed benzophenone derivatives of ginkgolides to identify their binding target in brain. Photolabeling of bovine hippocampus homogenates identified a series of alpha-tubulin isotypes. Selective photolabeling of alpha-tubulin over beta-tubulin, which is equally abundant in brain, suggested that ginkgolides might modulate microtubule biology differently than typical microtubule-binding agents, such as taxol. In fact, ginkgolide A did not affect microtubule polymerization or cell proliferation; instead, it inhibited detyrosination of alpha-tubulin and reorientation of microtubule-organizing centers. Taken together, the current findings indicate that ginkgolides constitute a new class of microtubule-binding agents with distinct effects on alpha-tubulin biology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据