Review
Biochemistry & Molecular Biology
Alazne Arrazola Sastre, Miriam Luque Montoro, Hadriano M. Lacerda, Francisco Llavero, Jose L. Zugaza
Summary: Rab and Arf GTPases play crucial roles in the pathogenesis and progression of neurodegenerative diseases, making them potential therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Petia Adarska, Luis Wong-Dilworth, Francesca Bottanelli
Summary: Molecular switches of the ADP-ribosylation factor (ARF) GTPase family play crucial roles in coordinating intracellular trafficking, including membrane deformation, cargo sorting, and recruitment of downstream coat proteins and effectors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Sander E. Van Der Verren, Giulia Zanetti
Summary: Sar1, a small GTPase protein of the ARF family, plays a role in cargo concentration and membrane deformation by associating with the endoplasmic reticulum membrane and recruiting COPII components. The regulation of Sar1's GTP hydrolysis and its role in coat assembly, as well as the mechanism of Sar1-induced membrane deformation and scission, are still not fully understood, especially in higher organisms with a wider range of secreted cargoes. This review focuses on these aspects and highlights the additional specialization in higher eukaryotes, along with the outstanding questions regarding the orchestration of Sar1 functions.
Article
Cell Biology
Kana Fujibayashi, Joji Mima
Summary: Arf-family small GTPases play crucial roles in membrane trafficking and tethering processes. Arf6 can act as a membrane tether by physically linking distinct lipid bilayers, while Arf1 exhibits weaker membrane tethering capability under current experimental conditions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Editorial Material
Cell Biology
Yonglun Zeng, Liwen Jiang
Summary: This study identified a plant-specific nexus in regulating autophagosome biogenesis in Arabidopsis thaliana.
Review
Cell Biology
Kem A. Sochacki, Justin W. Taraska
Summary: Researchers use emerging CLEM techniques to study the assembly of protein coats inside cells. Current and developing CLEM technologies are poised to answer fundamental questions about protein coat architecture at the nanoscale.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Amel Ben Saad, Virginie Vauthier, Martine Lapalus, Elodie Mareux, Evangeline Bennana, Anne-Marie Durand-Schneider, Alix Bruneau, Jean-Louis Delaunay, Emmanuel Gonzales, Chantal Housset, Tounsia Ait-Slimane, Francois Guillonneau, Emmanuel Jacquemin, Thomas Falguieres
Summary: ABCB4 is an ABC transporter crucial for phosphatidylcholine secretion into bile. Research has identified RAB10 as a novel molecular partner of ABCB4, influencing its plasma membrane expression and function. Understanding the interactions between RAB10 and ABCB4 may lead to the development of novel therapies for liver diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Brittany J. Bisnett, Brett M. Condon, Caitlin H. Lamb, George R. Georgiou, Michael Boyce
Summary: COPII, an ancient and essential pathway in eukaryotes, mediates forward trafficking of protein and lipid cargoes from the endoplasmic reticulum. Despite its broad significance, major aspects of COPII trafficking remain incompletely understood. Recent studies have highlighted the role of post-transcriptional mechanisms in regulating the COPII pathway.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Eric Herrmann, Jan-Hannes Schaefer, Stephan Wilmes, Christian Ungermann, Arne Moeller, Daniel Kuemmel
Summary: The endosomal system is vital for eukaryotic cells and controls cellular processes such as cell growth and metabolic signaling. Rab7 is a key regulator of endosomal maturation and lysosomal function in metazoans. The Mon1-Ccz1-Bulli (MCBulli) complex, which belongs to the tri-longin domain (TLD) family, activates Rab7. However, the role of Bulli in this complex has not been fully understood. Cryo-electron microscopy revealed the structure of MCBulli and showed that Bulli is associated with Mon1 and Ccz1 but does not directly impact the activity or interactions of the complex. The structural analysis suggests that Bulli serves as a recruitment platform for additional regulators of endolysosomal trafficking.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Daniel Lagunas-Gomez, Carolina Yanez-Dominguez, Guadalupe Zavala-Padilla, Charles Barlowe, Omar Pantoja
Summary: The endoplasmic reticulum (ER) is the start site of the secretory pathway, and phosphorylation and dephosphorylation of a protein called Erv14 are important for its correct functioning. Mimicking phosphorylation prevents Erv14 from entering COPII vesicles, leading to growth delay, abnormal growth in sec mutants, changes in ER structure, and altered localization of plasma membrane transporters. Dephosphorylated mimic has less deleterious effects but still affects ER structure and slows cell growth. These findings suggest that phosphorylation and dephosphorylation play a crucial role in ER protein trafficking.
JOURNAL OF CELL SCIENCE
(2023)
Review
Immunology
Tatiana M. Clemente, Rajendra K. Angara, Stacey D. Gilk
Summary: Obligate intracellular pathogens can either reside freely in the host cell cytoplasm or be confined in a membrane-bound vacuole. The pathogens in membrane-bound vacuoles are protected from the innate immune system and antimicrobial drugs, but they face challenges such as obtaining membrane components, maintaining a suitable microenvironment, acquiring essential nutrients from the host cell, and manipulating the host cell through a specialized secretion system.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Cell Biology
Elizabeth B. Thomas, Renaldo Sutanto, Richard S. Johnson, Han-Wei Shih, Germain C. M. Alas, Jana Krtkova, Michael J. MacCoss, Alexander R. Paredez
Summary: A proposed staging system for encysting Giardia based on CWP1 staining of ESVs, along with molecular distinctiveness of maturing ESVs through tracking GlRab GTPases, and the relationship between GlRac and ESV stages. Additionally, potential interactors of GlRac identified through proteomic studies could serve as additional markers for ESV stages.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Zehui Lei, Jing Wang, Lingqiang Zhang, Cui Hua Liu
Summary: Membrane trafficking relies on the regulation of small GTPases, which can be modulated through post-translational modifications such as ubiquitination. Dysregulation of small GTPase ubiquitination may lead to various human diseases, highlighting the importance of understanding the molecular mechanisms involved.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Hongyuan Jin, Yuanxin Tang, Liang Yang, Xueqiang Peng, Bowen Li, Qin Fan, Shibo Wei, Shuo Yang, Xinyu Li, Bo Wu, Mingyao Huang, Shilei Tang, Jingang Liu, Hangyu Li
Summary: Rab GTPases play essential roles in cancer progression by regulating membrane trafficking, and targeting Rab GTPases has the potential to become a novel approach for cancer treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Srimeenakshi Sankaranarayanan, Carl Haag, Patrick Petzsch, Karl Koehrer, Anna Matuszynska, Kathi Zarnack, Michael Feldbruegge
Summary: Fungal pathogens rely on RNA-binding proteins for gene expression regulation. This study discovers the specific role of the RNA-binding protein Khd4 in regulating membrane trafficking during pathogenic development of Ustilago maydis, providing new insights into the pathogenicity mechanisms of fungi.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)