Modification of the Method of Spectrophotometric Determination of Chlorophyll A in the Suspended Matter of Water Bodies

This article describes a modification of the spectrophotometric method for the determination of chlorophyll a in seston (suspended matter), which is convenient for hydroecological monitoring. It is proposed to use nuclear filters, as they have a number of advantages over fiberglass and bulk filters. An important difference of our approach from the standard methods is the use of the short-term (up to 30 min) drying of nuclear filters with seston in a desiccator at a temperature of 50-55oC immediately after the suspended matter is deposited on the filter. A comparative analysis of the standard methodology and the proposed modification for assessing the content of chlorophyll and phaeopigments in laboratory cultures of algae (Euglena gracilis, Chlorella vulgaris) and natural plankton from a natural reservoir was carried out.