Amycolatopsis nivea sp. nov., isolated from a Yellow River sample

A novel actinobacterium, designated strain CFH 50261(T), was isolated from a sediment sample of the Yellow River. The taxonomic position of the strain was investigated by using a polyphasic approach. Cells of strain CFH S0261(T) were Gram-reaction-positive, aerobic, non-motile. Growth occurs at 15-37 degrees C, pH 6.0-8.0 and with 0-9.0% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261(T) was a member of the genus Amycolatopsis. The 165 rRNA gene sequence similarity indicated that strain CFH 50261(T) is most closely related to the type strains of Amycolatopsis niigatensis LC11(T) (98.95%), Amycolatopsis echigonensis LC2(T) (98.81%) and Amycolatopsis albidoflavus IMSNU 22139(T) (98.73%). The whole-genome of CFH S0261(T) showed a G+C content of 69.5 mol%. The ANI values and in silica DDH values between CFH 50261(T) and the other species of the genus Amycolatopsis were found to be low (AN lb <90.61 % and DDH <53.40%). The cell wall diamino acid in the peptidoglycan of strain CFH 50261(T) was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose. The predominant menaquinone was MK-9(H-4). The major cellular fatty acids were C-16:0, iso-C-15:0 and iso-C-16:0.. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids. On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 50261(T) represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp. nov. is proposed. The type strain is CFH 50261(T) (=KCTC 39515(T)=CCTCC AA 2014028(T)).