Article
Plant Sciences
Xiaoran Yang, Ran Hu, Fujun Sun, Shulin Shen, Mengzhen Zhang, Yiwei Liu, Yi Zhang, Hai Du, Kun Lu, Cunmin Qu, Nengwen Yin
Summary: This study identified and characterized HKT genes in rapeseed, revealing their roles in response to abiotic stresses. Based on phylogenetic relationships and functional domain composition, the HKT members were classified into four subgroups. The expression profiles of BnaHKT genes varied under potassium, phytohormone, and heavy-metal treatment, suggesting their involvement in heavy-metal tolerance.
Article
Plant Sciences
Bei Han, Chuang Wang, Tao Wu, Junjun Yan, Aosheng Jiang, Yu Liu, Yu Luo, Hongmei Cai, Guangda Ding, Xu Dong, Philip J. White, Fangsen Xu, Sheliang Wang, Lei Shi
Summary: This study identified two vacuolar Pi influx transporters in Brassica napus and revealed their importance and distinct roles in cellular Pi homeostasis. These transporters also affected seed yield and traits in this plant species.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Engineering, Environmental
Zi Yang, Hai-Tao Wu, Hao Yang, Wan-Di Chen, Jia-Lan Liu, Fan Yang, Li Tai, Bin-Bin Li, Bo Yuan, Wen-Ting Liu, Yan-Feng Zhang, Yan-Rong Luo, Kun-Ming Chen
Summary: Phytoextraction is an environmentally friendly phytoremediation technology that reduces the total amount of heavy metals in soil. In this study, we identified three different heavy metal transporters in the hyperaccumulator Sedum pumbizincicola that can transport cadmium. By overexpressing these transporters in high biomass and environmentally adaptable crops, the ability to accumulate cadmium and other heavy metals was significantly increased. These findings offer effective solutions for phytoextraction in both cadmium and multiple heavy metal-contaminated soils.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Cuiping Zhang, Lijing Lu, Ruolin Gong, Xing Su, Fengbo Liu, Ru Zhang, Jihong Hu
Summary: Trihelix (TH) proteins, as plant-specific transcription factors, play important roles in plant growth, development, and stress responses. However, the function of TH genes in Brassica napus and their evolution and differentiation patterns in Brassica plants are still unclear. In this study, we identified and classified 455 TH genes in Brassica species and Arabidopsis. The analyses revealed the conservation and variation of TH protein motifs and gene structures. The expansion of TH genes in tetraploid species was found to be due to hetero-tetraploidization and gene duplication events. Comparative analysis showed significant expansion of GT-2 and SIP1 genes during evolution. Differential expression of BnaTH genes under various stresses suggests their involvement in stress responses. The presence of stress-responsive cis-elements in the promoter region indicates the potential of BnaTH genes to cope with diverse environments. qRT-PCR analyses confirmed the response of TH genes to abiotic stresses. These findings provide valuable information and candidate genes for further studying the role of TH genes in stress resistance of B. napus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Lu Han, Xiaohua Wu, Xinyu Zhang, Kailin Hou, Hongshan Zhang, Chenjia Shen
Summary: In this study, a cation-efflux gene named BjCET1 was cloned and characterized in Brassica juncea. The results suggest that BjCET1 plays an essential role in heavy metal ion homeostasis and hyper-accumulation.
Article
Agricultural Engineering
Ziyi Ye, Fei Hu, Weimeng Zhang, Da Fang, Kui Dong, Jun Cao
Summary: This study provides a comprehensive analysis of the AAT gene family in Brassica napus, revealing its evolutionary process and functions. The study shows that this gene family plays important roles in the growth and development, as well as the stress response of B. napus, laying the foundation for future functional validation.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Agronomy
Tingting Liu, Baolong Tao, Hanfei Wu, Jing Wen, Bin Yi, Chaozhi Ma, Jinxing Tu, Tingdong Fu, Lixia Zhu, Jinxiong Shen
Summary: Loss of function of the chloroplast development-related gene Bn.YCO affected plastid gene transcription in rapeseed seedlings, influencing chloroplast biogenesis.
Article
Plant Sciences
Sheza Ayaz Khilji, Zahoor Ahmad Sajid, Sidra Fayyaz, Anis Ali Shah, Adnan Noor Shah, Mamoona Rauf, Muhammad Arif, Seung Hwan Yang, Sajid Fiaz
Summary: This study aimed to investigate the effects of fulvic acid (FA) on the growth and stress tolerance of Brassica napus exposed to paper sludge (PS) in soil. The results showed that FA reduced metal uptake and improved antioxidant enzyme activity in plants grown at different concentrations of PS.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Shan Tang, Ning Guo, Qingqing Tang, Fei Peng, Yunhao Liu, Hui Xia, Shaoping Lu, Liang Guo
Summary: The study highlights the important role of BnaBASS2 in seed lipid metabolism and accumulation in Brassica napus, showing that gene manipulation can alter metabolites in seeds and influence lipid synthesis.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Zishu Xu, Yi Duan, Hui Liu, Mingchao Xu, Zhi Zhang, Ling Xu
Summary: The study identified and molecularly evolved the WAK gene family in Cruciferous species. A total of 178 WAK genes were identified, and a phylogenetic tree showed that Brassica rapa, Brassica oleracea, and Brassica napus genes were closely related. The WAK gene family was unevenly distributed in B. napus chromosomes, with the largest number of genes on chromosome C08. Expression analysis revealed variations in the expression patterns of the WAK gene family under different stress treatments. This study lays the foundation for further understanding the functional mechanisms of the WAK gene family in Brassica napus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Martina Komarkova, Zuzana Kovalikova, Jiri Simek, Adam Skarka, Jiri Tuma
Summary: Different rapeseed cultivars showed varying physiological responses and accumulation abilities to high doses of cadmium. Cadmium was found to accumulate more in roots than in shoots. CZL and Benefit had higher translocation factors at the lowest dose of cadmium. The content of potassium and calcium varied between treatments and cultivars. Minimal effects were observed on chlorophyll fluorescence parameters and relative chlorophyll content. High levels of salicylic acid and proline content were mainly found in Iwas, indicating its higher tolerance to cadmium.
PLANT SOIL AND ENVIRONMENT
(2022)
Article
Multidisciplinary Sciences
Tao Wu, Li Pan, Isaac Zipori, Jihua Mao, Rongbo Li, Yongpeng Li, Yongjie Li, Yuebo Jing, Haiyun Chen
Summary: This study demonstrates the effectiveness of native AMF in promoting the growth and phosphorus uptake of olive plantlets, highlighting their potential as inoculants for producing healthy and robust olive plantlets.
Article
Biochemistry & Molecular Biology
Yuyu Xie, Yunyou Nan, Ayub Atif, Wencong Hu, Yanfeng Zhang, Hui Tian, Yajun Gao
Summary: This study characterized the G-protein gene family in allotetraploid B. napus and analyzed their expression profiles in response to nitrogen deprivation. The results revealed the conservation of gene structure and protein properties among G alpha, G beta, and G gamma subunits. Collinearity analysis showed syntenic relationships between G-protein genes in B. napus and other Brassicaceae species. Expression profile analysis indicated organ-specific and nitrogen-responsive expression patterns of G-protein genes. These findings provide insight into the genetic dissection and biological functions of G-protein genes in B. napus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xueyuan Li, Sjur Sandgrind, Oliver Moss, Rui Guan, Emelie Ivarson, Eu Sheng Wang, Selvaraju Kanagarajan, Li-Hua Zhu
Summary: This study established a rapid and efficient protocol for the isolation, regeneration, and transfection of protoplasts of rapeseed, investigating different basal media, types and combinations of plant growth regulators, and protoplast culture duration. Optimized combinations in media led to up to 45% shoot regeneration, with a critical importance of culture duration on different media. The transfection protocol was also successfully optimized for high mutation frequency in rapeseed gene editing.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Engineering, Environmental
Zaid Ulhassan, Su Yang, Di He, Ali Raza Khan, Abdul Salam, Wardah Azhar, Sajid Muhammad, Skhawat Ali, Yasir Hamid, Imran Khan, Mohamed Salah Sheteiwy, Weijun Zhou
Summary: This study found that seed priming with silicon dioxide nanoparticles (SiO2 NPs) can alleviate the phytotoxicity of chromium (Cr) in Brassica napus seedlings. SiO2 NPs reduce the accumulation of Cr, enhance nutrient acquisition, improve photosynthetic performance, and boost plant immunity. SiO2 NPs have the potential to be used as stress-reducing agents for crops grown in Cr-contaminated areas.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Plant Sciences
Lijuan Wei, Hai Du, Xiaodong Li, Yonghai Fan, Mingchao Qian, Yali Li, Huiyi Wang, Cunmin Qu, Wei Qian, Xinfu Xu, Zhanglin Tang, Kai Zhang, Jiana Li, Kun Lu
Summary: This study provides a comprehensive analysis of gene function and homoeologous gene expression in different tissues at various developmental stages in Brassica napus. The findings suggest that certain gene functions are similar across different tissues, while transcription factor genes are more specific. Tissue-preferred genes have been identified and shown to play important roles in gene regulatory networks and hormone pathways. The study also reveals that biased expression of homoeologous genes is associated with structural divergence, the presence of transposable elements, and regulatory elements.
Article
Plant Sciences
Dashuang Yuan, Yin Zhang, Zhen Wang, Cunmin Qu, Dongming Zhu, Huafang Wan, Ying Liang
Summary: This study focused on the effects of BnKAT2 gene on the length of the main inflorescence and the number of siliques in Brassica napus. The overexpression of BnKAT2 was found to effectively increase the length of the main inflorescence and the number of siliques in both B. napus and transgenic Arabidopsis thaliana. The results suggest that BnKAT2 may regulate these traits through the auxin and cytokinin signaling pathways.
Article
Biochemistry & Molecular Biology
Liyuan Zhang, Bo Yang, Chao Zhang, Huan Chen, Jinxiong Xu, Cunmin Qu, Kun Lu, Jiana Li
Summary: In this study, the Argonaute gene family in Brassica napus was comprehensively analyzed, and a total of 10 AtAGOs, 13 BolAGOs, 11 BraAGOs, and 24 BnaAGOs were identified. Expression analysis revealed that the miR168a-AGO1s module negatively regulates yield traits, while the miR403-AGO2s module positively influences yield. This study provides a theoretical basis for breeding high-yielding rapeseed varieties through the manipulation of miRNA-AGOs modules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Plant Sciences
Umer Mahmood, Xiaodong Li, Yonghai Fan, Wei Chang, Yue Niu, Jiana Li, Cunmin Qu, Kun Lu
Summary: Crop production is facing challenges from increasing food demand and climate change. Advances in omics techniques and machine learning algorithms can accelerate crop breeding to cope with these challenges. Plant breeding and genetic engineering can develop nutrient sufficient and climate-resilient crops.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yunshan Tang, Guorui Zhang, Xinyue Jiang, Shulin Shen, Mingwei Guan, Yuhan Tang, Fujun Sun, Ran Hu, Si Chen, Huiyan Zhao, Jiana Li, Kun Lu, Nengwen Yin, Cunmin Qu
Summary: We conducted a genome-wide association study on six glucosinolate metabolites in rapeseed and identified 113 candidate intervals significantly associated with these compounds. Within the genomic regions linked to the association peaks, we predicted 187 candidate genes involved in glucosinolate biosynthesis and novel genes. This study provides insights into the genetic basis of glucosinolate biosynthesis in rapeseed and could aid marker-based breeding for improved seed quality in Brassica species.
Article
Plant Sciences
Mingwei Guan, Xiangtian Shi, Si Chen, Yuanyuan Wan, Yunshan Tang, Tian Zhao, Lei Gao, Fujun Sun, Nengwen Yin, Huiyan Zhao, Kun Lu, Jiana Li, Cunmin Qu
Summary: Yellow seed coat in rapeseed is a desirable trait and understanding the genetic mechanism behind it can improve the quality of this oilseed crop. Through transcriptome profiling, differentially expressed genes related to seed development were identified and enriched in processes like carbohydrate and lipid metabolism, photosynthesis, and embryo development. Furthermore, candidate genes involved in seed coat color were discovered and 25 transcription factors were identified to potentially regulate the synthesis of flavonoids and phenylpropanoids, thus influencing seed color formation. These findings provide valuable insights into candidate gene function in seed development and lay the foundation for further research on the yellow-seeded trait in rapeseed.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Bo Yang, Liyuan Zhang, Sirou Xiang, Huan Chen, Cunmin Qu, Kun Lu, Jiana Li
Summary: This study conducted a comprehensive investigation of TPS gene family in rapeseed, identifying 35 BnTPSs, 14 BoTPSs, and 17 BrTPSs. These genes were classified into three subfamilies. Changes in gene structures were found to potentially lead to differences in expression profiles and functional differentiation. Additionally, the study discovered that certain BnTPSs were significantly upregulated after drought stress and that differentially expressed genes exhibited varying expression patterns in source and sink tissues. These findings provide a basis for further understanding the roles of TPSs in both yield and drought resistance in rapeseed.
Review
Cardiac & Cardiovascular Systems
Shahid Ullah Khan, Sumbul Saeed, Amnah Mohammed Alsuhaibani, Sumaya Fatima, Khalil ur Rehman, Umber Zaman, Muneeb Ullah, Moamen S. Refat, Kun Lu
Summary: This review highlights the significance of genome-wide association studies (GWAS) in advancing our understanding of the etiology of coronary artery disease (CAD) and improving drug development strategies. It discusses the challenges in identifying causal genes and interpreting the connections between disease pathology and risk variants, as well as the novel insights into the biological mechanisms behind CAD based on GWAS outcomes. It also explores the successful discovery of new treatment targets through the integration of omics data and systems genetics strategies.
CURRENT PROBLEMS IN CARDIOLOGY
(2023)
Article
Engineering, Environmental
Weihong Xu, He Huang, Xiaodong Li, Mei Yang, Sunlin Chi, Yu Pan, Nannan Li, Andrew H. Paterson, Yourong Chai, Kun Lu
Summary: In this study, a genome-wide association study was performed to identify genes associated with Cd accumulation in pepper fruits. The results revealed a genomic region containing a homolog of the HMA1 gene, which is involved in Cd transport. Additionally, transcriptome analysis uncovered differentially expressed genes related to carbohydrate metabolism and photosynthesis in fruits with high Cd accumulation. The study also demonstrated the significant role of the CaHMA1 gene in regulating Cd content in pepper plants.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Multidisciplinary Sciences
Cunmin Qu, Meichen Zhu, Ran Hu, Yongchao Niu, Si Chen, Huiyan Zhao, Chengxiang Li, Zhen Wang, Nengwen Yin, Fujun Sun, Zhiyou Chen, Shulin Shen, Guoxia Shang, Yan Zhou, Xingying Yan, Lijuan Wei, Liezhao Liu, Bin Yi, Jinmin Lian, Jiang Li, Zhanglin Tang, Ying Liang, Xinfu Xu, Rui Wang, Jiaming Yin, Huafang Wan, Hai Du, Wei Qian, Yourong Chai, Qingyuan Zhou, Yajun He, Silin Zhong, Xiao Qiu, Hao Yu, Hon-Ming Lam, Kun Lu, Fuyou Fu, Jiana Li
Summary: In this study, high-quality genomes of yellow-seeded (GH06) and black-seeded (ZY821) rapeseed were assembled, and a quantitative trait locus (QTL) controlling seed color was finely mapped. The BnA09MYB47a gene encoding a transcription factor was identified as the causal gene for the yellow-seed trait. Functional studies revealed that sequence variation of BnA09MYB47a leads to the functional divergence between yellow- and black-seeded rapeseed.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Xiaoran Yang, Ran Hu, Fujun Sun, Shulin Shen, Mengzhen Zhang, Yiwei Liu, Yi Zhang, Hai Du, Kun Lu, Cunmin Qu, Nengwen Yin
Summary: This study identified and characterized HKT genes in rapeseed, revealing their roles in response to abiotic stresses. Based on phylogenetic relationships and functional domain composition, the HKT members were classified into four subgroups. The expression profiles of BnaHKT genes varied under potassium, phytohormone, and heavy-metal treatment, suggesting their involvement in heavy-metal tolerance.
Article
Agronomy
Xiaodong Li, Xumei Liu, Yonghai Fan, Shengting Li, Mengna Yu, Mingchao Qian, Yuling Chen, Hongqiao Chen, Xinchun Li, Bei Liu, Xinfu Xu, Cunmin Qu, Jiana Li, Kun Lu
Summary: In this study, a target capture sequencing SNP genotyping platform named BnaPan50T was developed for detecting genetic variation and constructing gene maps in rapeseed. The platform has a high density of SNPs and a high proportion of functional genes, promising wide application in genetic research and breeding of rapeseed.
Article
Agronomy
Ran Hu, Meichen Zhu, Si Chen, Chengxiang Li, Qianwei Zhang, Lei Gao, Xueqin Liu, Shulin Shen, Fuyou Fu, Xinfu Xu, Ying Liang, Liezhao Liu, Kun Lu, Hao Yu, Jiana Li, Cunmin Qu
Summary: Yellow seed trait is a desirable characteristic in rapeseed, and this study identified a transcription factor gene BnbHLH92a that can inhibit the synthesis of anthocyanin and proanthocyanidins in rapeseed seeds, leading to reduced levels of these compounds.
Article
Biotechnology & Applied Microbiology
Umer Mahmood, Xiaodong Li, Mingchao Qian, Yonghai Fan, Mengna Yu, Shengting Li, Ali Shahzad, Cunmin Qu, Jiana Li, Liezhao Liu, Kun Lu
Summary: In this study, a comparative transcriptome analysis was conducted to better understand the pod shattering mechanism in rapeseed. Senescence-associated genes, such as Bna.A05ABI5 and Bna.C03ERF/AP2-3, were found to play a crucial role in regulating the shattering process. Additionally, the role of auxin transport by Bna.WAG2 in enhancing shatter resistance was observed.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2023)
