Article
Biochemistry & Molecular Biology
Summer A. Helmi, Leili Rohani, Ahmed R. Zaher, Youssry M. El Hawary, Derrick E. Rancourt
Summary: Bone healing is a well-organized process regulated by multiple factors. The Notch signaling pathway plays a significant role in controlling bone mesenchymal stem cell differentiation. Inhibiting the Notch pathway can greatly enhance the differentiation of both embryonic and induced pluripotent stem cells, potentially improving overall healing outcomes. Stem cell transplantations are prominent candidates for treating critical-sized bone defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Agnieszka Fus-Kujawa, Barbara Mendrek, Anna Trybus, Karolina Bajdak-Rusinek, Karolina L. Stepien, Aleksander L. Sieron
Summary: Induced pluripotent stem cells (iPSCs) show potential in gene therapy and disease modeling. To safely apply this technology in clinical settings, optimal reprogramming factors and vectors must be considered to reduce the risk of insertional tumorigenesis.
Review
Endocrinology & Metabolism
Alberto Posabella, Andrea B. Alber, Hendrik J. Undeutsch, Raoul A. Droeser, Anthony N. Hollenberg, Laertis Ikonomou, Darrell N. Kotton
Summary: Stem cell-based therapies hold potential for reconstituting organ function, particularly in addressing hypothyroidism. Research has shown promise in engineering thyroid follicular epithelial cells to potentially cure the disease, but technical and biological challenges need to be overcome to achieve long-term cure through cell- and/or gene-based therapies.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Cell Biology
Glyn Nigel Stacey, Jie Hao
Summary: In recent years, significant progress has been made in China in establishing hPSCs for the manufacture of cell therapies. The National Stem Cell Resource Center and its partner organizations have over 600 hPSC lines formally recorded, with 47 of them also registered for European Commission funded research projects. This national and international coordination aims to promote the translation of Chinese hPSC-based products into clinical use according to established standards.
CELL PROLIFERATION
(2022)
Article
Cardiac & Cardiovascular Systems
Jessica C. Garbern, Gabriela O. Escalante, Richard T. Lee
Summary: Cell replacement therapy using pluripotent stem cell-derived cardiomyocytes (PSC-CMs) is still in the research phase, with various challenges that need to be addressed before successful clinical translation can be achieved.
TRENDS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Michelle Vanessa Kamga Kapchoup, Juergen Hescheler, Filomain Nguemo
Summary: Hydroxychloroquine (HDQ) has been widely studied for its potential use in the treatment and prevention of COVID-19. This antimalarial drug has shown beneficial effects on stem cell proliferation and cardiomyocyte activity, but it can also be toxic at high concentrations. Identifying risk factors and understanding the cardiotoxic profile of HDQ is challenging.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Cell & Tissue Engineering
Minhao Chen, Yingjuan Li, Longfei Xiao, Guangchun Dai, Panpan Lu, Yunfeng Rui
Summary: This study reveals that enhanced expression of Wnt5a in aged TSPCs leads to senescence, and inhibiting Wnt5a can attenuate senescence through modulating the JAK-STAT signaling pathway. Furthermore, Ror2 acts as the functional receptor of Wnt5a in TSPCs senescence by potentiate the JAK-STAT signaling pathway.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Emma Claire James, Eva Tomaskovic-Crook, Jeremy Micah Crook
Summary: The regenerative capacity of cardiomyocytes is insufficient for functional recovery, leading to ischaemic heart disease as a major cause of cardiovascular deaths. While human-induced pluripotent stem cells (hiPSCs) show potential for modeling heart disease and replacement therapy, challenges remain with the mixed populations and immature properties of hiPSC-derived cardiomyocytes, which are being addressed by innovative bioengineering technologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Immunology
Sho Tanosaki, Shugo Tohyama, Yoshikazu Kishino, Jun Fujita, Keiichi Fukuda
Summary: Pluripotent stem cells (PSCs) have promising applications in regenerative therapy, drug discovery, and disease modeling, but their metabolism differs greatly from that of somatic cells, requiring distinct features to sustain their unique properties.
INFLAMMATION AND REGENERATION
(2021)
Article
Biotechnology & Applied Microbiology
M. R. Poorna, Sagi Pravallika, Aditya Ashok, S. Sudhindran, M. V. Thampi, Praveen Kerala Varma, Ullas Mony
Summary: This study explored a natural biomaterial, CNF, as an alternative substrate to promote the differentiation of iPSCs to cardiomyocytes, replacing the expensive and difficult to produce Vn substrate. The results showed that differentiated cardiomyocytes expressed similar markers on CNF and Vn substrates, indicating the potential of CNF as an extracellular matrix in tissue engineering applications.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Cardiac & Cardiovascular Systems
Tim Stuedemann, Judith Roessinger, Christoph Manthey, Birgit Geertz, Rajiven Srikantharajah, Constantin von Bibra, Aya Shibamiya, Maria Koehne, Antonius Wiehler, J. Simon Wiegert, Thomas Eschenhagen, Florian Weinberger
Summary: This study reveals the mechanism of how transplanted cardiomyocytes actively contribute to heart function, supporting the hypothesis that the delivery of new force-generating myocardium can serve as a regenerative therapeutic strategy.
Review
Biochemistry & Molecular Biology
Matin Chehelgerdi, Fereshteh Behdarvand Dehkordi, Mohammad Chehelgerdi, Hamidreza Kabiri, Hosein Salehian-Dehkordi, Mohammad Abdolvand, Sharareh Salmanizadeh, Mohsen Rashidi, Anoosha Niazmand, Saba Ahmadi, Sara Feizbakhshan, Saber Kabiri, Nasimeh Vatandoost, Tayebeh Ranjbarnejad
Summary: The emergence of iPSCs has revolutionized stem cell research and opened up new avenues for cancer treatment. iPSCs provide a unique platform for investigating the origin of cancer and can be used for personalized treatment and drug testing. This review article discusses the science behind iPSCs, the limitations and challenges in iPSC-based cancer therapy, ethical and social implications, and a comparative analysis with other stem cell types for cancer treatment. It also explores the applications of iPSCs in tumorigenesis and the future of iPSC research in this field.
Article
Cell & Tissue Engineering
Naruphong Phunikom, Nittaya Boonmuen, Pakpoom Kheolamai, Kanoknetr Suksen, Sirikul Manochantr, Chairat Tantrawatpan, Duangrat Tantikanlayaporn
Summary: Andrographolide (AP) has been shown to promote proliferation, osteogenic differentiation, and bone formation of placenta-derived mesenchymal stem cells. It may be an effective novel agent for improving placenta-derived MSCs and stem cell-based therapy for bone regeneration. The compound enhances Wnt/beta-catenin signaling and osteoblast-specific gene expressions, promoting bone formation and increasing osteocalcin levels.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cardiac & Cardiovascular Systems
Sophia E. Silver, Ryan W. Barrs, Ying Mei
Summary: Cardiovascular disease is a leading cause of global mortality, and alternative cardiac regenerative therapies, such as hPSC-derived cardiomyocytes, show promising therapeutic potential for heart repair. Despite challenges and limitations, solutions are being explored to address these issues towards successful clinical translation and future perspectives on hPSC-CM transplantation.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cell Biology
Tzu-Cheng Sung, Yi-Peng Jiang, Jhe-Yu Hsu, Qing-Dong Ling, Hao Chen, Suresh S. Kumar, Yung Chang, Shih-Tien Hsu, Qingsong Ye, Akon Higuchi
Summary: This study successfully generated universal hiPSCs without gene editing and evaluated their resistance to immune reactions, demonstrating that the cells maintained stable characteristics even after continuous passages.
CELL PROLIFERATION
(2021)
Article
Cardiac & Cardiovascular Systems
Takuro Arimura, Antoine Muchir, Masayoshi Kuwahara, Sachio Morimoto, Taisuke Ishikawa, Cheng-Kun Du, Dong-Yun Zhan, Shu Nakao, Noboru Machida, Ryo Tanaka, Yoshihisa Yamane, Takeharu Hayashi, Akinori Kimura
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2018)
Review
Neurosciences
Tomoe Y. Nakamura, Shu Nakao, Shigeo Wakabayashi
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2019)
Article
Biochemical Research Methods
Tasuku Tsukamoto, Takahiro Sogo, Tomoe Ueyama, Shu Nakao, Yukihiro Harada, Dai Ihara, Yuka Akagi, Yasuyuki S. Kida, Koji Hasegawa, Teruyuki Nagamune, Masahiro Kawahara, Teruhisa Kawamura
BIOTECHNOLOGY JOURNAL
(2020)
Article
Multidisciplinary Sciences
Joseph Yanni, Alicia D'Souza, Yanwen Wang, Ning Li, Brian J. Hansen, Stanislav O. Zakharkin, Matthew Smith, Christina Hayward, Bryan A. Whitson, Peter J. Mohler, Paul M. L. Janssen, Leo Zeef, Moinuddin Choudhury, Min Zi, Xue Cai, Sunil Jit R. J. Logantha, Shu Nakao, Andrew Atkinson, Maria Petkova, Ursula Doris, Jonathan Ariyaratnam, Elizabeth J. Cartwright, Sam Griffiths-Jones, George Hart, Vadim V. Fedorov, Delvac Oceandy, Halina Dobrzynski, Mark R. Boyett
SCIENTIFIC REPORTS
(2020)
Article
Cardiac & Cardiovascular Systems
Pietro Mesirca, Shu Nakao, Sarah Dalgas Nissen, Gabriella Forte, Cali Anderson, Tariq Trussell, Jue Li, Charlotte Cox, Min Zi, Sunil Logantha, Sana Yaar, Helena Cartensen, Isabelle Bidaud, Luke Stuart, Luca Soattin, Gwilym M. Morris, Paula A. da Costa Martins, Elizabeth J. Cartwright, Delvac Oceandy, Matteo E. Mangoni, Thomas Jespersen, Rikke Buhl, Halina Dobrzynski, Mark R. Boyett, Alicia D'Souza
Summary: The study found that atrioventricular block in athletes is mainly caused by electrical remodeling. Trained animals and mice showed atrioventricular node dysfunction, characterized by prolonged PR intervals and reduced expression of key ion channels. miRNAs such as miR-211-5p and miR-432 were identified as direct regulators of Ca(V)1.2 and HCN4.
CIRCULATION RESEARCH
(2021)
Article
Veterinary Sciences
S. Nakao, A. J. Atkinson, T. Motomochi, D. Fukunaga, H. Dobrzynski
Summary: A 6-month-old female cat presented with complex cardiac anomalies including biatrial and biventricular dilation, with branching abnormalities in the aorta. The cat died of severe congestive heart failure, with postmortem diagnosis confirming the arterial trunk abnormalities through micro-computed tomography analysis. Micro-computed tomography proved to be a valuable tool for ex vivo diagnosis of complex cardiac anomalies in this case.
JOURNAL OF VETERINARY CARDIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Sunil Jit R. J. Logantha, Xue J. Cai, Joseph Yanni, Caroline B. Jones, Robert S. Stephenson, Luke Stuart, Gillian Quigley, Oliver Monfredi, Shu Nakao, Il-Young Oh, Tobias Starborg, Ashraf Kitmitto, Akbar Vohra, Robert C. Hutcheon, Antonio F. Corno, Jonathan C. Jarvis, Halina Dobrzynski, Mark R. Boyett, George Hart
Summary: Severe heart failure causes significant effects on Purkinje fibers, including dysfunction, impaired ventricular repolarization, and electrical remodeling, which may increase the risk of fatal arrhythmias in patients.
CIRCULATION-HEART FAILURE
(2021)
Article
Cell Biology
Yukihiro Harada, Toru Tanaka, Yuji Arai, Yoshie Isomoto, Atsushi Nakano, Shu Nakao, Akihiro Urasaki, Yusuke Watanabe, Teruhisa Kawamura, Osamu Nakagawa
Summary: SGK1 is predominantly expressed in endothelial cells of mouse embryos, and its expression is maintained through at least two ETS-regulated enhancers, located in the proximal and distal regions. The presence of these enhancers is essential for the responsiveness to ERG, FLI1, and ETS1 proteins.
Review
Biochemistry & Molecular Biology
Mark R. Boyett, Joseph Yanni, James Tellez, Annalisa Bucchi, Pietro Mesirca, Xue Cai, Sunil Jit R. J. Logantha, Claire Wilson, Cali Anderson, Jonathan Ariyaratnam, Luke Stuart, Shu Nakao, Eman Abd Allah, Sandra Jones, Matthew Lancaster, Robert Stephenson, Natalie Chandler, Matthew Smith, Carol Bussey, Oliver Monfredi, Gwilym Morris, Rudi Billeter, Matteo E. Mangoni, Henggui Zhang, George Hart, Alicia D'Souza
Summary: This review summarizes the discovery of the funny current If in the heart several decades ago, as well as its important role in the sinus node and atrioventricular node. Studies have shown that the transcription and translation of Hcn genes play a crucial role in pacemaking in the sinus node and conduction in the atrioventricular node under normal physiological conditions and pathological states.
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tomoaki Ishida, Tomoe Ueyama, Dai Ihara, Yukihiro Harada, Sae Nakagawa, Kaho Saito, Shu Nakao, Teruhisa Kawamura
Summary: Induced pluripotent stem cells (iPSCs) have great potential in regenerative medicine and disease modeling, but their reprogramming efficiency is still low. In this study, researchers investigated the role of miR-17-92 in iPSC induction. They found that upregulation of miR-17-92 increased reprogramming efficiency, while deletion of the miR-17-92 cluster decreased efficiency. Further analysis revealed that miR-17-92 targets genes PTEN and p21, and their downregulation is disrupted by introducing the 3′ UTR of PTEN and p21, suggesting they act as competing endogenous RNAs (ceRNA). These findings provide insight into the underlying mechanism of iPSC reprogramming.
Article
Immunology
Takahiro Sogo, Shu Nakao, Tasuku Tsukamoto, Tomoe Ueyama, Yukihiro Harada, Dai Ihara, Tomoaki Ishida, Masato Nakahara, Koji Hasegawa, Yuka Akagi, Yasuyuki S. Kida, Osamu Nakagawa, Teruyuki Nagamune, Masahiro Kawahara, Teruhisa Kawamura
Summary: In this study, an inexpensive molecule-responsive differentiation-inducing chimeric antigen receptor (diCAR) was generated, which successfully activated Wnt3a signaling and promoted the differentiation of mouse embryonic stem cells into cardiomyocytes. This research is expected to be an economical and powerful tool for stem cell-based regeneration therapy.
INFLAMMATION AND REGENERATION
(2023)
Article
Cardiac & Cardiovascular Systems
Shu Nakao, Dai Ihara, Koji Hasegawa, Teruhisa Kawamura
EUROPEAN CARDIOLOGY REVIEW
(2020)
Review
Immunology
Tomoaki Ishida, Shu Nakao, Tomoe Ueyama, Yukihiro Harada, Teruhisa Kawamura
INFLAMMATION AND REGENERATION
(2020)
Proceedings Paper
Computer Science, Interdisciplinary Applications
Jue Li, Pietro Mesirca, Alicia D'Souza, Yanwen Wang, Shu Nakao, Charlotte Cox, Jules Hancox, Matteo Mangoni, Mark Boyett
2018 COMPUTING IN CARDIOLOGY CONFERENCE (CINC)
(2018)