Article
Oncology
Ting Wang, Xue-Ying Huang, Su-Jun Zheng, Ye-Ying Liu, Si-Si Chen, Feng Ren, Jun Lu, Zhong-Ping Duan, Mei Liu
Summary: This study identified novel proteins in AFP-negative HCC and evaluated using autoantibodies for predicting hepatocarcinogenesis.
FRONTIERS IN ONCOLOGY
(2021)
Article
Fisheries
Rui Xue, Dinglong Yang, Yijing Han, Qinyou Deng, Xin Wang, Xiangquan Liu, Jianmin Zhao
Summary: The 14-3-3 zeta and 14-3-3 epsilon proteins play important roles in innate immunity against V. harveyi infection in mollusks, with their differential expression leading to different immune responses.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Neurosciences
Cuixian Li, Shen Huang, Jin Peng, Tianguo Hong, Chun Zhou, Jie Tang
Summary: In this study, a new cellular protein, 14-3-3 zeta, was found to interact with the beta subunit of GABA(A)Rs and BIG1, and co-localize with them in hippocampal neurons. Overexpression of 14-3-3 zeta increased the surface expression of BIG1 and its binding with GABA(A)R. Depletion of 14-3-3 zeta or BIG1 significantly decreased GABA(A)R expression at the cell surface and inhibited GABA-gated influx of chloride ions.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Oncology
S. Breedy, W. S. Ratnayake, L. Lajmi, R. Hill, M. Acevedo-Duncan
Summary: Neuroblastoma is a common cancer in children under the age of 1 and has varying prognosis based on age and cell differentiation. Our study identified two protein kinase inhibitors with potential therapeutic effects against NB cells. These inhibitors induced cell apoptosis and inhibited EMT. The Akt1/NF-κB and TGF-β pathways were found to be regulated by aPKCs, and their association with 14-3-3 and Smad2/3 could be diminished by aPKC inhibitors. Both inhibitors show promise as neuroblastoma therapeutics and require further research.
FRONTIERS IN ONCOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Phillip Zhu, Stanislau Stanisheuski, Rachel Franklin, Amber Vogel, Cat Hoang Vesely, Patrick Reardon, Nikolai N. Sluchanko, Joseph S. Beckman, P. Andrew Karplus, Ryan A. Mehl, Richard B. Cooley
Summary: 14-3-3 proteins dimerize and bind phosphorylated clients to regulate their function. By introducing stable mimics of phosphorylated amino acids, researchers can study 14-3-3 function effectively. In this study, the authors improved a previous genetic code expansion system to incorporate nonhydrolyzable phosphoserine into proteins. They successfully produced biologically relevant proteins with the modified amino acid and investigated the effects of phosphorylation on protein interactions and function.
ACS CENTRAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Nam Ah Kim, Ga Yeon Noh, Shavron Hada, Kyung Jun Na, Hee-Jung Yoon, Ki-Woong Park, Young -Min Park, Seong Hoon Jeong
Summary: In this study, a comparative study was conducted to evaluate the effectiveness of N-acetyl-L-arginine (NALA) and arginine monohydrochloride in liquid protein formulations. The results showed that NALA retained the transition temperature better and reduced protein aggregation and immunogenicity compared to arginine monohydrochloride.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Oncology
Chao Gao, Si-wei Wang, Jia-cheng Lu, Xiao-qiang Chai, Yuan-cheng Li, Peng-fei Zhang, Xiao-yong Huang, Jia-bin Cai, Yi-min Zheng, Xiao-jun Guo, Guo-ming Shi, Ai-wu Ke, Jia Fan
Summary: This study reveals the important role of KSR2 in the occurrence and development of HCC, and high expression of KSR2 is associated with poor prognosis. Mechanistically, the interaction between KSR2 and 14-3-3ζ plays a key role in the activation of the MAPK signaling pathway and drug resistance in HCC cells. Therefore, the KSR2-14-3-3ζ interaction may be a therapeutic target to enhance the sensitivity of HCC to sorafenib.
BIOMARKER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Akshatha Ganne, Meenakshisundaram Balasubramaniam, Nirjal Mainali, Paavan Atluri, Robert J. Shmookler J. Reis, Srinivas Ayyadevara
Summary: The presence of 14-3-3 proteins in cerebrospinal fluid can serve as a specific biomarker for neuronal damage-related diseases. In this study, the enrichment of 14-3-3 proteins in brain aggregates associated with Alzheimer's disease was observed. Interaction partners of 14-3-3 proteins were identified, and drugs targeting the protein complexes showed potential in inhibiting aggregation and rescuing behavioral deficits in disease models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Seppe Leysen, Rebecca Jane Burnley, Elizabeth Rodriguez, Lech-Gustav Milroy, Lorenzo Soini, Carolyn J. Adamski, Larissa Nitschke, Rachel Davis, Tomas Obsil, Lucas Brunsveld, Tom Crabbe, Huda Yahya Zoghbi, Christian Ottmann, Jeremy Martin Davis
Summary: Expansion of the polyglutamine tract in Ataxin-1's N terminus is the main cause of SCA1, while the phosphorylation event on residue serine 776 in the C-terminal part also plays a crucial role in disease development. 14-3-3 proteins have a direct anti-aggregation effect on Ataxin-1 by interfering with its dimerization, which is particularly important in the context of SCA1 where soluble mutant Ataxin-1 drives pathology.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Immunology
Melanie C. McKell, Rebecca R. Crowther, Stephanie M. Schmidt, Michelle C. Robillard, Rachel Cantrell, Maria A. Lehn, Edith M. Janssen, Joseph E. Qualls
Summary: Macrophages rely on L-arginine for anti-Mtb defense, not only for NO production but also for regulating metabolism and other host defense mechanisms. Inhibition of glycolysis or mTOR reduces macrophage control of Mtb, as well as macrophage number and viability in the presence of L-arginine. L-arginine is essential for macrophage function in combating intracellular pathogens.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Mingda Zhao, Yibing Zhang, Longfei Li, Xiaobin Liu, Wenping Zhou, Chunhui Wang, Yufu Tang
Summary: Our study found that KHDRBS3 is highly expressed in human HCC tissues and predicts poor prognosis. Knockdown of KHDRBS3 inhibits tumor growth, reduces glycolysis, enhances sensitivity to doxorubicin, and induces apoptosis in HCC cells. On the contrary, overexpression of KHDRBS3 promotes malignant behavior. KHDRBS3 interacts with YWHAZ, and the promotion of proliferation and glycolysis and the inhibition of apoptosis caused by KHDRBS3 overexpression can be reversed by silencing 14-3-3ζ.
CANCER CELL INTERNATIONAL
(2023)
Article
Geriatrics & Gerontology
Yuanyuan Lu, Alzheimer's Dis Neuroimaging Initiat
Summary: This study revealed the potential diagnostic and prognostic utility of CSF 14-3-3ζ in the AD continuum. High 14-3-3ζ predicted conversion from MCI to AD and was associated with related biomarkers.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Maria del Carmen Fernandez-Ramirez, Kevin Kan-Shing Ng, Margarita Menendez, Douglas V. Laurents, Ruben Hervas, Mariano Carrion-Vazquez
Summary: Understanding the early stages of amyloid formation is crucial for developing effective therapeutic strategies. Although the mechanism of tau protein aggregation, a key feature of neurodegenerative diseases, is still unclear, this study used single-molecule force spectroscopy to investigate the conformational landscape of the monomeric tau repeat domain. The researchers discovered different conformational states influenced by mutations and the chemical environment, with pathological mutations and an amyloidogenic milieu favoring expanded conformations. The findings provide insight into the complex aggregation process of tau protein and its relevance to different tauopathies.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Plant Sciences
Feiyan Chen, Lin Chen, Weifeng Liang, Zhengguang Zhang, Jiao Li, Wan Zheng, Zhu Zhu, Jiapeng Zhu, Yunan Zhao
Summary: The study identified a direct interaction between the ginsenoside metabolite PPD and the 14-3-3 zeta protein in brain tissues, with mutations in specific residues of the protein leading to decreased affinity with PPD. This finding could aid in the development of small-molecular compounds that bind to the 14-3-3 zeta protein based on dammarane-type triterpenoid structure.
JOURNAL OF GINSENG RESEARCH
(2021)
Article
Polymer Science
Hosoowi Lee, Jun Ho Hwang, Dajung Lee, Inhye Kim, Jiwon Park, Eunji Lee, Woo-Dong Jang
Summary: A porphyrin tripod connected through a triazole bridge was prepared as a monomeric building block for guest-induced supramolecular polymerization. The addition of 1,3,5-tri(pyridin-4-yl)benzene or Cl- induced the formation of fibrous and networked supramolecular polymers, which could be depolymerized by adding Cu(ClO4)2 or AgNO3.
Article
Biochemistry & Molecular Biology
Natalia A. Chebotareva, Svetlana G. Roman, Vera A. Borzova, Tatiana B. Eronina, Valeriya V. Mikhaylova, Boris Kurganov
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Tatiana B. Eronina, Valeriya V. Mikhaylova, Natalia A. Chebotareva, Vladimir V. Shubin, Sergey Y. Kleymenov, Boris Kurganov
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Biochemistry & Molecular Biology
Natalia A. Chebotareva, Tatiana B. Eronina, Valeriya V. Mikhaylova, Svetlana G. Roman, Kristina Tugaeva, Boris Kurganov
Summary: This study investigated the effect of trehalose on the quaternary structure and anti-aggregation activity of alpha B-Cr. The results showed that trehalose affected the nucleation stage of Phb thermal aggregation and increased the adsorption capacity of alpha B-Cr during the aggregation process. Additionally, trehalose stabilized the dimeric form of Phb and enhanced the interaction between alpha B-Cr and the target protein.
BIOCHEMISTRY-MOSCOW
(2022)
Article
Multidisciplinary Sciences
Mahtab Hafizi, Natalia A. Chebotareva, Maryam Ghahramani, Faezeh Moosavi-Movahedi, Seyed Hossein Khaleghinejad, Boris Kurganov, Ali Akbar Moosavi-Movahedi, Reza Yousefi
Summary: A new mutation in the alpha B-crystallin protein has been discovered, which can lead to myofibrillar myopathy, cataracts, and cardiomyopathy. The mutation significantly impacts the structure, stability, and chaperone-like activity of the protein.
Article
Biochemistry & Molecular Biology
Vera A. Borzova, Natalia A. Chebotareva, Nikolai N. Sluchanko, Sergey Yu Kleymenov, Kira A. Markossian, Boris Kurganov
Summary: Chemical chaperones are low-molecular compounds that counteract protein aggregation. The aggregation process of bovine liver glutamate dehydrogenase (GDH) at different temperatures was studied, and the unfolding of the protein was found to be the rate-limiting stage of the overall aggregation process. The anti-aggregation activity of chemical chaperones was quantified, and arginine ethyl ester showed the highest activity.
Article
Biochemistry & Molecular Biology
Tatiana B. Eronina, Valeriya V. Mikhaylova, Natalia A. Chebotareva, Sergey Y. Kleymenov, Anastasia V. Pivovarova, Boris I. Kurganov
Summary: Chemical chaperones are small molecules that enhance protein stability and folding. They have significant effects on the stability, oligomeric state, and aggregation of muscle glycogen phosphorylase b (Phb).
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Tatiana B. Eronina, Valeriya V. Mikhaylova, Natalia A. Chebotareva, Kristina V. Tugaeva, Boris I. Kurganov
Summary: Protein-protein interactions are important for biological processes, and chemical chaperones can influence the structure of proteins, affecting their interactions with target proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Vera A. Borzova, Svetlana G. Roman, Anastasiya V. Pivovarova, Natalia A. Chebotareva
Summary: Intracellular proteins aggregation can be enhanced under stress. Heat-shock proteins (HSPs) and accumulation of osmolytes are cellular protective mechanisms. The antiaggregation activity of HSPB5 can be affected by crowding agents and osmolytes, and different additives can either improve or impair its activity against different protein targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
V. Mikhaylova, T. Eronina, B. Kurganov