期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 160, 期 -, 页码 979-990出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.05.216
关键词
Degradation; Tremella fuciformis polysaccharide; Antioxidant activity
资金
- National Natural Science Foundation of China [31972047, 31871831]
- LiaoNing Revitalization Talents Program [XLYC1807040]
- Liaoning BaiQianWan Talents Program, the Liaoning Support Program for Innovative Talents [LR2019065]
- Scientific research project of Liaoning Provincial Department of Education [LSNJC201902]
The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied. The main process parameters of degradation were evaluated and the structural changes and antioxidant activities of TFP before and after US/H2O2 were analyzed. The results showed that the degradation effect of US/H2O2 was significantly higher than that of US or H2O2 alone, and the degradation of TFP was dependent on the duration of its exposure to US, the ultrasonic amplitude, and the H2O2 and TFP concentrations. US/H2O2 reduced the molecular weight (from 8.14 x 10(5) Da to 1.27 x 10(4) Da) and particle size (from 710 nm to 182 nm) of the TFPwithin 50 min and narrowed itsmolecularweight and particle size distribution. High performance liquid chromatography, Fourier-transforminfrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFPmolecules without changing the primary structure and monosaccharide composition of TFP. Additionally, the degraded TFPs with low molecular weight exhibited a higher antioxidant activity than the original TFP. These findings suggest that the US/H2O2 treatment is a simple and effective method to prepare a TFP of low molecular weight and high bioactivity. (C) 2020 Elsevier B.V. All rights reserved.
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