期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 148, 期 -, 页码 56-67出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.01.125
关键词
Nizamuddinia zanardinii; Fucoidan; Immunostimulation; Structure; Signaling pathway
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2018R1A6A1A03023584]
- Tarbiat Modares University [IG-39807]
Polysaccharides from Nizamuddinia zanardinii were extracted using water at elevated temperature and fractionated by a DEAE Sepharose F column yielding four fractions (F-1-F-4). Crude and fractions were composed of neutral sugars (50.8-57.4%), proteins (10.8-18.1%), sulfates (7.5-17.3%) and uronic acids (3.5-7.7%). Various levels of galactose (13.4-44.4%), fucose (34.1-40.1%), mannose (14.1-33.2%) and xylose (7.4-15.2%) formed the building blocks of the polysaccharide structures. The weight average molecular weights (M-w) of polysaccharides varied between 40.3 and 1254.4 x 10(3) g/mol. F-3 polysaccharide was the most active fraction stimulating RAW264.7 murine macrophage cells to secrete NO, TNF-alpha, IL-1 beta and IL-6, and activating NK cells to release TNF-alpha, INF-gamma, granzyme-beta, perforin, NKG2D and FasL through NF-kappa B and MAPKs signaling pathways. Highly-branched F-3 poly- saccharide mainly consisted of (1 -> 2)-Fucp, (1 -> 2,3)-Manp, (1 -> 3)-Galp, (1 -> 2)-Manp, (1 -> 3)-Manp, (1 -> 2,3,4)-Manp and (1 -> 2,3,6)-Manp residues with great amount of (-> 1)-Fucp and (-> 1)-Xylp. Sulfates substituted at C-2 of fucose and galactose residues. Overall, fucoidan from N. zanardinii showed immense potency in boosting immune system through macrophages and NK cells activations and therefore suitable for further exploration in immune-mediated biomedical applications. (C) 2020 Elsevier B.V. All rights reserved.
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