Article
Biotechnology & Applied Microbiology
Chao Du, Xiang Zhao, Wen Song, Nisha He, Sijing Jiang, Yuling Zhou, Guimin Zhang
Summary: Through multiple engineering strategies, a recombinant Pichia pastoris with high-level secretion of AMCase was successfully constructed, showing significantly improved chitinase activity under high-density fermentation conditions. The hydrolysis of colloidal chitin by the recombinant AMCase not only could reduce water consumption significantly, but also promote seed germination and seedling growth of wheat and rice.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Lilang Li, Xuejing Mao, Fuli Deng, Yonghua Wang, Fanghua Wang
Summary: In this study, the thermostability of a novel phospholipase D (PLD) from marine Moritella sp. JT01 (MsPLD) was improved by applying disulfide bond engineering strategy. The mutant with two additional disulfide bonds showed a significantly longer half-life time at the optimum reaction temperature, as well as increased melting temperature. Unexpectedly, the mutant also exhibited higher specific activity and catalytic efficiency. Molecular dynamics simulations indicated that the introduction of double disulfide bonds rigidified the global structure of the mutant, leading to improved thermostability. The mutant also showed a higher synthesis capacity for phosphatidic acid (PA), reducing the reaction time needed to reach equilibrium. These findings pave the way for the further application of MsPLD in the food and pharmaceutical industries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Heng Zhang, Wenxin Zhai, Lin Lin, Ping Wang, Xiangyang Xu, Wei Wei, Dongzhi Wei
Summary: A thermostable alpha-amylase gene AmySN was cloned from Geobacillus stearothermophilus, with a mutant AmyS2 showing enhanced temperature tolerance and stability when immobilized on a support compared to the wild type. The mutant enzyme also exhibited higher efficiency in maize starch saccharification, leading to increased reducing sugar yield and improved cost-effectiveness.
Article
Biochemistry & Molecular Biology
Linshu Jiao, Huibing Chi, Bingjie Xia, Zhaoxin Lu, Xiaomei Bie, Haizhen Zhao, Fengxia Lu, Meirong Chen
Summary: The thermostability of Acinetobacter soli L-asparaginase was improved by mutating two non-conserved cysteine residues, resulting in enhanced efficiency in food pre-processing.
Article
Biochemistry & Molecular Biology
Katarzyna Biniek-Antosiak, Magdalena Bejger, Joanna Sliwiak, Daniel Baranowski, Ahmed S. A. Mohammed, Dmitri I. I. Svergun, Wojciech Rypniewski
Summary: Chitin degradation involves the important step of deacetylation. The analysis of a deacetylase from the extremophile Pyrococcus chitonophagus reveals that it is a hexameric, zinc-containing metalloenzyme with high structural stability and specific catalytic activity. This study provides important insights into the enzymatic mechanism of chitin degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Composites
Ying Yang, Shiyuan Wang, Changcheng Wang, Hongbo Yin, Mokun Chen, Longbo Luo, Maozhu Tang, Yun-Xiang Xu
Summary: Adding a small quantity of heterocyclic aramid to epoxidized natural rubber can simultaneously enhance the mechanical properties and thermostability of dynamically cross-linked rubber, resulting in improved comprehensive performances of vitrimer rubber.
COMPOSITES COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Wenzhuo Tian, Ziyang Zhang, Cuiping Yang, Piwu Li, Jing Xiao, Ruiming Wang, Peng Du, Nan Li, Junqing Wang
Summary: In this study, a xylanase gene, CFXyl3, was cloned from Cellulomonas flavigena and four hybrid xylanases with enhanced thermostability were engineered. These hybrid xylanases, especially EcsXyl4, showed improved optimal temperature and melting temperature, and could be applied in prebiotic xylooligosaccharide manufacturing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yingying Zhang, Yongchun Liu, Yonggang Liu, Ping Zuo, Shuting Miao, Bowen Hu, Yu Kang, Wei Liu, Qingmin Yang, Hao Ren, Peng Yang
Summary: Proteins can form robust bioadhesive materials through an α-helix-mediated interfacial adhesion model. Reduction of disulfide bonds in proteins leads to the gathering of α-helices at the solid/liquid interface, exposing hydrophobic residues and disrupting the hydration layer. This model provides a unique strategy for preparing protein nanofilms with well-defined layering, exhibiting strong adhesion and resistance to various conditions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Materials Science, Paper & Wood
Mohamed A. Al Abboud, Aisha M. H. Al-Rajhi, Abdel-Rahman M. Shater, Mohamed M. Alawlaqi, Abdullah Mashraqi, Samy Selim, Soad K. Al Jaouni, Tarek M. Abdel Ghany
Summary: Strategies based on halo-and thermostable enzymes show promise for biotechnological applications. In this study, three fungal isolates were used to produce chitinase under different NaCl concentrations. The results showed that C. cladosporioides was the main chitinase producer at high NaCl concentration. The chitinase produced by C. cladosporioides exhibited good thermal stability and halostability. It also showed inhibitory activity against certain fungi and had insecticidal activity against C. pipiens larvae.
Article
Biotechnology & Applied Microbiology
Laura Navone, Thomas Vogl, Pawarisa Luangthongkam, Jo-Anne Blinco, Carlos H. Luna-Flores, Xiaojing Chen, Juhani von Hellens, Stephen Mahler, Robert Speight
Summary: This study presents a thermostable variant of the E. coli AppA phytase, ApV1, with an extra non-consecutive disulfide bond, which shows promising thermostability but leads to decreased production in Pichia pastoris. Co-expression of folding chaperones like protein disulfide bond isomerase (Pdi) significantly increased the production of ApV1 and restored yields to levels comparable with the wild-type enzyme. Engineering for enhanced enzymatic properties may result in folding complexity and reduced production in microbial systems, highlighting the importance of developing improved production strains in parallel to achieve desirable levels of recombinant protein for industrial processes.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biochemistry & Molecular Biology
He Li, Zijia Lu, Meng-Shu Hao, Alma Kvammen, Annie R. Inman, Vaibhav Srivastava, Vincent Bulone, Lauren S. Mckee
Summary: There is a demand for heat-resistant enzymes in biomass-processing industries. Researchers have focused on discovering new thermostable enzymes and developing methods to stabilize existing enzymes. Attaching a stable non-catalytic domain to an enzyme can protect the biocatalyst from thermal denaturation. In this study, a chitin-degrading enzyme from GH family 18 was found to be appended to two CBM domains from family 92, which increased the enzyme's thermal stability without contributing to enzyme-substrate interactions. This finding suggests that CBM92 domains may have a natural role in enzyme stabilization and could be relevant for enzyme design in high-temperature biorefineries.
Article
Chemistry, Multidisciplinary
Tian-Ci Wang, Ling Zhu, Shiwei Luo, Zhong-Sheng Nong, Pu-Sheng Wang, Liu-Zhu Gong
Summary: Asymmetric functionalization of the C(sp(3))-H bond using chiral phosphoramidite-palladium catalysis was successfully achieved in this study, leading to the synthesis of enantioenriched beta-amino-gamma,delta-unsaturated carbonyl derivatives and providing a key intermediate for the asymmetric synthesis of Focalin. The research also revealed an umpolung reactivity of N-allylimines through a concerted proton and two-electron transfer process.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Polymer Science
Jinchuang Rong, Jiang Zhong, Wanlong Yan, Maochen Liu, Yalei Zhang, Yongluo Qiao, Changqing Fu, Fei Gao, Liang Shen, Haifeng He
Summary: The novel self-healable waterborne polyurethanes with synergistic reversible units of aliphatic disulfide bonds and quadruple hydrogen bonding have been successfully prepared, showing superior mechanical properties and self-repairing efficiency.
Article
Polymer Science
Wencong Zhang, Minhui Wang, Jiahui Zhou, Yeming Sheng, Min Xu, Xiaolin Jiang, Yuanhao Ma, Xun Lu
Summary: By synthesizing thermoplastic polyurethanes with multiple dynamic bonds, self-healing elastomers with high strength and efficiency were successfully prepared. The self-healing mechanism can be explained from the perspective of aggregate structure, degree of microphase separation, and molecular dynamics.
EUROPEAN POLYMER JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Fatima Akram, Zuriat Jabbar, Amna Aqeel, Ikram ul Haq, Shahbaz Tariq, Kausar Malik
Summary: Chitinases have the ability to degrade chitin into useful products, making them important for waste management and industrial applications. They also have potential as biocontrol agents in plant disease management. The development of thermostable and recombinant chitinases, as well as the use of transgenic plants, have further expanded their applications in industry and agriculture.
MOLECULAR BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Pei Xu, Xiao-Ling Wu, Xiao-Xiao Guo, Jian Tang, Min-Hua Zong, Wen-Yong Lou
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2019)
Article
Chemistry, Multidisciplinary
Hui-Hui Su, Ze-Wang Guo, Xiao-Ling Wu, Pei Xu, Ning Li, Min-Hua Zong, Wen-Yong Lou
Article
Biotechnology & Applied Microbiology
Xiao-Yang Ou, Xiao-Ling Wu, Fei Peng, Pei Xu, Shi-Yu Zhang, Min-Hua Zong, Wen-Yong Lou
JOURNAL OF BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Xiao-Yang Ou, Xiao-Ling Wu, Fei Peng, Ying-Jie Zeng, Hui-Xian Li, Pei Xu, Gu Chen, Ze-Wang Guo, Ji-Guo Yang, Min-Hua Zong, Wen-Yong Lou
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Article
Biotechnology & Applied Microbiology
Lin-Shang Zhang, Pei Xu, Mei-Yun Chu, Min-Hua Zong, Ji-Guo Yang, Wen-Yong Lou
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Zi-Fu Ni, Ying-Jie Zeng, Pei Xu, Ze-Wang Guo, Xiao-Yang Ou, Fei Peng, Ji-Guo Yang, Min-Hua Zong, Wen-Yong Lou
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2020)
Article
Chemistry, Physical
Ping Wei, Ze-Wang Guo, Xiao-Ling Wu, Shan Liang, Xiao-Yang Ou, Pei Xu, Min-Hua Zong, Ji-Guo Yang, Wen-Yong Lou
MOLECULAR CATALYSIS
(2019)
Article
Biotechnology & Applied Microbiology
Xiaoyang Ou, Fei Peng, Xiaoling Wu, Pei Xu, Minhua Zong, Wenyong Lou
BIOCHEMICAL ENGINEERING JOURNAL
(2020)
Review
Biotechnology & Applied Microbiology
Pei Xu, Shi-Yu Zhang, Zhi-Gang Luo, Min-Hua Zong, Xiao-Xi Li, Wen-Yong Lou
Summary: Pullulanase is an important enzyme in the alpha-amylase family that plays a key role in debranching and hydrolyzing starch, leading to improved product quality, increased productivity, and reduced production costs. This review discusses the progress in characterizing pullulanases, as well as the heterologous expression in different microbial hosts and enzyme production regulation. Additionally, protein engineering and immobilization strategies are explored to facilitate the industrial application of pullulanases in the future.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Pei Xu, Shan Liang, Min-Hua Zong, Wen-Yong Lou
Summary: Ionic liquids have been considered promising reaction media for biocatalysis due to their unique characteristics, and great developments have been achieved in this field in recent years. This review provides illustrative examples of biocatalysis in ILs, discusses possible mechanisms, ways to regulate catalytic efficiency, and offers an outlook on expanding biocatalysis in ILs.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Chemistry, Multidisciplinary
Ze-Wang Guo, Xiao-Yang Ou, Pei Xu, Hui-Fang Gao, Liao-Yuan Zhang, Min-Hua Zong, Wen-Yong Lou
Article
Biochemistry & Molecular Biology
Lei Pu, Ying-Jie Zeng, Pei Xu, Fang-Zhou Li, Min-Hua Zong, Ji-Guo Yang, Wen-Yong Lou
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Biotechnology & Applied Microbiology
Hui-Hui Su, Fei Peng, Pei Xu, Xiao-Ling Wu, Min-Hua Zong, Ji-Guo Yang, Wen-Yong Lou
BIORESOURCES AND BIOPROCESSING
(2019)
Article
Chemistry, Applied
Jiaxin Gao, Xiaoyang Ou, Pei Xu, Minhua Zong, Wenyong Lou
CHINESE JOURNAL OF CATALYSIS
(2018)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)