Article
Chemistry, Analytical
Baha Ondes, Fatma Akpinar, Murat Uygun, Mihrican Muti, Deniz Aktas Uygun
Summary: A highly stable urea biosensor was prepared by immobilizing urease enzyme onto nanoparticles, showing a detection limit of 0.77 mu M and a linear concentration range of 0.01 mM-500 mM. The biosensor system exhibited high repeatability and retained a certain level of activity after long-term storage. Interference from common interferents was found to be negligible, and the biosensor was proven suitable for detecting urea in artificial human serum.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Nur Izzati Zakaria, Rosmawani Mohammad, Sharina Abu Hanifah, Azrilawani Ahmad
Summary: In this study, an optical biosensor for urea based on functionalised calcium carbonate nanoparticles was successfully developed. The biosensor showed a colorimetric indication of urea concentrations and had a wide linear response range, low detection limit, good reproducibility, and no interference from major cations. The fabricated biosensor demonstrated no significant difference with the standard method for urea determination in urine samples.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Engineering, Environmental
Salhah D. Al-Qahtani, Hanan K. Alzahrani, Omer A. Azher, Zeid O. Owidah, Matokah Abualnaja, Turki M. Habeebullah, Nashwa M. El-Metwaly
Summary: A high-sensitivity viscose fabric was developed for colorimetric recognition of urea in fluids using co-encapsulated calcium alginate nanoparticles. Extracted anthocyanin from red-cabbage and urease enzyme were studied for their role in the fabrication of a biosensor on fabrics. The colorimetric changes of the fabric were monitored with increasing urea concentration, showing a shift in absorbance spectra and color variations.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Materials Science, Multidisciplinary
Reena K. Sajwan, Purnima Sharma, G. B. V. S. Lakshmi, Pratima R. Solanki
Summary: A fluorescence Turn-On based strategy using MPA-CdSe QDs was utilized for urea determination, with a detection range from 1 to 120 mM and a lower detection limit of 1 mM. The pH sensitivity of MPA-CdSe QDs was key in detecting urea through the enzymatic reaction with urease and the release of ammonia.
Article
Chemistry, Analytical
Cheol-Kyun Choi, Samy M. Shaban, Byeong-Seok Moon, Do-Gi Pyun, Dong-Hwan Kim
Summary: This study developed a rapid and reliable colorimetric urea biosensor assisted by a smartphone, with high selectivity, sensitivity, and reliability. It enables direct diagnosis of human urine and paves the way for daily monitoring systems of renal and hepatic dysfunction diseases.
ANALYTICA CHIMICA ACTA
(2021)
Article
Nanoscience & Nanotechnology
Mengyi Xu, Ye Zhu, Shanhui Gao, Zhixing Zhang, Yuxuan Gu, Xiaoya Liu
Summary: A 3D flexible biosensor based on nanocomposites was developed for detecting glucose in sweat, showing high sensitivity and detection limit, making it suitable for clinical analysis.
ACS APPLIED NANO MATERIALS
(2021)
Article
Polymer Science
Mohamed H. El-Newehy, Hany El-Hamshary, Waheed M. Salem
Summary: Cellulose, a widely-used material, was utilized as a carrier for urease enzyme and tricyanofuran hydrazone to develop a novel fibrous film sensor. The sensor can detect urea visually with rapid response time and low detection limits. The morphology of the nanofibrous films was characterized using various analytical tools, showcasing the successful immobilization of urease enzyme and TCFH onto cellulose nanofibers.
Article
Polymer Science
Kalpana Settu, Pin-Tzu Chiu, Yu-Ming Huang
Summary: The study presents a flexible laser-induced graphene-based electrochemical biosensor for glucose detection, showing good amperometric responses over a wide linear range of up to 8 mM. This biosensor, constructed on a polymer substrate by laser engraving, demonstrated high sensitivity and selectivity, making it a promising option for low-cost and rapid glucose detection.
Article
Engineering, Chemical
Mukhtar Nuhu Yahya, Huseyin Gokcekus, Derin Orhon, Bulent Keskinler, Ahmet Karagunduz, Philip Isaac Omwene
Summary: This study investigated the enzymatic hydrolysis of urea using urease enzyme in a membrane reactor, finding that higher ionic strength and concentrations of NH4SO2 and ammonium nitrogen inhibit urea hydrolysis over time. A sustainable alternative for converting urea to ammonia by utilizing urease in an EMR was demonstrated.
Article
Physics, Applied
Takao Ono, Miho Kannaka, Yasushi Kanai, Naruto Miyakawa, Ayumi Shinagawa, Shin-ichi Nakakita, Yohei Watanabe, Shota Ushiba, Shinsuke Tani, Yasuo Suzuki, Masahiko Kimura, Daichi Chiba, Kazuhiko Matsumoto
Summary: Graphene biosensors coated with gas-permeable silicone elastomer show stable responses to target ammonia molecules in solution, due to the suppression of external disturbances. The relationship between ammonia production and conductivity changes of the biosensor was clarified using the combination of dissociation equilibrium, adsorption isotherm, and enzyme kinetics. These findings lay the foundation for practical applications of stable graphene biosensors based on a reasonable response model.
JAPANESE JOURNAL OF APPLIED PHYSICS
(2023)
Article
Nanoscience & Nanotechnology
Hyun-June Jang, Xiaoyu Sui, Wen Zhuang, Xiaodan Huang, Min Chen, Xiaolei Cai, Yale Wang, Byunghoon Ryu, Haihui Pu, Nicholas Ankenbruck, Kathleen Beavis, Jun Huang, Junhong Chen
Summary: This study proposes a new nanomaterial-based field-effect transistor (FET) configuration for stable and reliable clinical applications. By investigating the surface characteristics and electrochemical instability of two-dimensional reduced graphene oxide (rGO), highly efficient detection of SARS-CoV-2 spike proteins is achieved.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Adria Martinez-Avino, Lusine Hakobyan, Ana Ballester-Caudet, Yolanda Moliner-Martinez, Carmen Molins-Legua, Pilar Campins-Falco
Summary: The proposed sensing device utilizes a solid sensing membrane composed of PDMS composite for in situ analysis of ammonia and urea in water and urine samples, with satisfactory strategies applied. Qualitative and quantitative analyte detection is achieved through diffuse reflectance measurements and digital image processing after the colorimetric reaction, showing a linear relationship between sensor response and analyte concentration, and validation through the Berthelot reaction-based thymol-PDMS sensor.
Article
Biochemical Research Methods
Jia An, Meizhu Chen, Guoyi Liu, Yongqin Hu, Rubing Chen, Ying Lyu, Sanjiv Sharma, Yufei Liu
Summary: In this study, CsPbBr3 perovskite quantum dots were successfully encapsulated in poly(styrene/acrylamide) microspheres, creating PDPS composites with stable photoluminescence and water solubility. These composites were used to construct a real-time pH monitoring platform that showed a good linear relationship within the pH range of 4-12 and was further extended for urea and urease detection. The detection limits for urea and urease were found to be 1.67 μM and 2.1 mU/mL, respectively, demonstrating the expanding applications of perovskite materials.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Multidisciplinary
Siti Nur Ashakirin, M. Aniq Shazni M. Haniff, M. Hazani M. Zaid, Mohd Farhanulhakim M. Razipwee, Ebrahim Mahmoudi
Summary: In this study, a label-free detection scheme based on a silver reduce graphene oxide modified working electrode was developed for the detection of urea. The sensor showed a wide linear range, high sensitivity and selectivity, and demonstrated good stability and reproducibility in artificial urine.
Article
Engineering, Electrical & Electronic
Anita Ahmadi, Seyyed Mehdi Khoshfetrat, Shima Kabiri, Lida Fotouhi, Parisa Seyed Dorraji, Kobra Omidfar
Summary: Nanocellulose fiber-based papers are ideal for developing electrochemical paper-based analytical devices due to their excellent characteristics; a new ePAD based on cellulose nanofibers for determining glucose concentration from whole blood samples was described by the authors; the biosensing motif showed high sensitivity, reproducibility, selectivity, and stability, making it a versatile platform for analyzing other biomarkers.
IEEE SENSORS JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Denise Biagini, Maria Franzini, Paolo Oliveri, Tommaso Lomonaco, Silvia Ghimenti, Andrea Bonini, Federico Vivaldi, Lisa Macera, Laurence Balas, Thierry Durand, Camille Oger, Jean-Marie Galano, Fabrizio Maggi, Alessandro Celi, Aldo Paolicchi, Fabio Di Francesco
Summary: “The role of oxylipins in COVID-19 inflammation has been poorly understood. However, a recent study found that oxylipins can differentiate between samples collected from COVID-19 wards and ICU. Patients in ICU showed lower levels of anti-inflammatory and pro-resolving lipid mediators, indicating impaired inflammation response. This study highlights the importance of targeting lipid mediators for a better understanding of COVID-19 inflammation and the potential for biomarker-based prediction models.”
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Andrea Idili, Andrea Bonini, Claudio Parolo, Ruslan Alvarez-Diduk, Fabio Di Francesco, Arben Merkoci
Summary: In this study, a reagentless electrochemical sensing platform was designed using the chemistry and programmability of DNA, enabling the rapid and efficient detection of antibodies and proteins. By modifying a DNA nanostructure and incorporating recognition elements and tag molecules, specific binding with the target and generation of electrochemical signal were achieved. The sensor could detect nanomolar concentrations of antibodies and proteins in untreated biological fluids within a short time.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Analytical
Tana Sebechlebska, Eva Vaneckova, Marta Katarzyna Choinska-Mlynarczyk, Tomas Navratil, Lukasz Poltorak, Andrea Bonini, Federico Vivaldi, Viliam Kolivoska
Summary: Fused deposition modeling 3D printing using electrically conductive filaments is an attractive tool for manufacturing sensing devices. This study explores the capabilities of 3D printed electrodes to measure electric properties of materials. The authors demonstrate the functionality of a bimaterial FDM-3DP platform for sensing conductivity and permittivity of liquids by impedance measurements. Additionally, they propose an original idea of using impedance measurements to investigate dimensions of 3D printed channels as base structures of microfluidic devices, complemented by optical microscopic analysis.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Marco Mattonai, Tommaso Nacci, Francesca Modugno
Summary: This article reviews the current analytical strategies for the detection and quantitation of tire and road wear particles (TRWPs) in environmental samples. It discusses the crucial aspects and challenges in the analysis, highlighting the selection of detection and quantitation markers, the variations in tire formulation, and the influence of sample matrix and composition on the analytical response.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Giulia Giaganini, Mario Cifelli, Denise Biagini, Silvia Ghimenti, Andrea Corti, Valter Castelvetro, Valentina Domenici, Tommaso Lomonaco
Summary: Microplastics and nanoplastics are a major environmental issue due to their ubiquity and potential danger to ecosystems and human life. This study investigates the effect of temperature and light on five types of reference polymers using a solar box. A multi-analytical approach was used to analyze the degradation products of these microplastics.
Article
Chemistry, Analytical
Marco Mattonai, Lucia Andrei, Marian Virgolici, Erika Ribechini
Summary: We used EGA-MS to analyze different varieties of ambers and obtained information on their non-polymeric and polymeric components. The results showed characteristic signals for the different fractions and allowed us to classify most of the samples. Principal component analysis differentiated between succinite and rumanite ambers. EGA-MS is a reliable technique for studying the molecular composition of ambers.
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
(2023)
Article
Chemistry, Analytical
Irene Bertelli, Marco Mattonai, Jacopo La Nasa, Erika Ribechini
Summary: In this study, the interactions between beeswax and resinous materials under heating were investigated to understand their effect on adhesive properties, and compared with archaeological samples to gain insights into ancient technological knowledge. The results suggested that thermal pre-treatment of archaeological mixtures led to the formation of hybrid species between resin diterpenes and the beeswax compounds, resulting in a material with new chemical features.
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
(2023)
Article
Biochemical Research Methods
Alessio Lenzi, Denise Biagini, Silvia Ghimenti, Federico M. Vivaldi, Pietro Salvo, Fabio Di Francesco, Tommaso Lomonaco
Summary: Variations in salivary short-chain fatty acids and hydroxy acids levels can reflect the dysbiosis of human gut microbiota, which plays a role in the onset of heart failure disease. This study presents a reliable and simple method for determining these metabolites in stimulated saliva samples, using in-situ derivatization and sorptive extraction coupled to gas chromatography-tandem mass spectrometry. The optimized protocol was successfully applied to evaluate the salivary levels of these acids in different groups of patients. Rating: 9/10.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Electrochemistry
E. Herrera, J. S. Riva, S. E. Urreta, M. Del C. Aguirre
Summary: This article describes the nucleation and growth mechanisms of bimetallic Sm-Co magnetic nanowires and nanotubes, which are electrocrystallized into hard anodic alumina templates. The electrodeposition process mainly proceeds under diffusional control, with current decreasing with time. Suitable models adapted to bimetallic phases are used to describe the experimental current/time curves and the morphology of the nanowires and nanotubes observed in SEM images.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Biochemical Research Methods
F. M. Vivaldi, S. Reale, S. Ghimenti, D. Biagini, A. Lenzi, T. Lomonaco, F. Di Francesco
Summary: Solid-phase sorption is commonly used for gas sample analysis as it allows preconcentration of target analytes and sample storage. Adding internal standards (ISs) can reduce analytical variability and improve reliability.
JOURNAL OF BREATH RESEARCH
(2023)
Article
Biology
Aidonis Rammos, Aris Bechlioulis, Petros Kalogeras, Chris J. Watson, Pietro Salvo, Tommaso Lomonaco, Olga Kardakari, Evanthia E. Tripoliti, Yorgos Goletsis, Dimitris I. Fotiadis, Christos S. Katsouras, Lampros K. Michalis, Katerina K. Naka
Summary: This study found that saliva NT-proBNP correlates with serum values in patients with acute decompensated HF, but cannot differentiate between HF and other causes of dyspnea.
Article
Chemistry, Inorganic & Nuclear
Cristiana Cesari, Beatrice Berti, Marco Bortoluzzi, Cristina Femoni, Tiziana Funaioli, Federico Maria Vivaldi, Maria Carmela Iapalucci, Stefano Zacchini
Summary: Heterometallic Chini-type clusters [Pt6-xNix(CO)(12)](2-) (x = 0-6) with different Pt/Ni compositions were obtained by reacting [Pt-6(CO)(12)](2-) with Ni-clusters. The site preference of Pt and Ni within their metal cages has been computationally investigated.
DALTON TRANSACTIONS
(2023)
Article
Engineering, Environmental
Greta Biale, Jacopo La Nasa, Lorenzo Fiorentini, Alessio Ceccarini, Diego Carnaroglio, Marco Mattonai, Francesca Modugno
Summary: Sampling, separation, detection, and characterization of micro- and nanoplastic pollutants is crucial for assessing their impact on ecosystems. However, there is still limited understanding of the interaction and exchange mechanisms between these pollutants and the environment. Recent studies have emphasized the importance of evaluating the chemical species associated with the presence of microplastics in the environment. Researchers have developed a method combining microwave-assisted extraction and analytical pyrolysis-GC-MS to quantify microplastics and associated pollutants.
ENVIRONMENTAL SCIENCE-ADVANCES
(2023)