期刊
BIOTECHNOLOGY LETTERS
卷 38, 期 3, 页码 477-481出版社
SPRINGER
DOI: 10.1007/s10529-015-1995-x
关键词
Biocatalysis; Permeabilized cells; Schizosaccharomyces pombe; Selective oxidation; UDP-glucose-6-dehydrogenase; UDP-glucuronate
资金
- BMBF (Federal Ministry of Education and Research [031P7238]
Objectives To use permeabilized cells of the fission yeast, Schizosaccharomyces pombe, that expresses human UDP-glucose 6-dehydrogenase (UGDH, EC 1.1.1.22), for the production of UDP-glucuronic acid from UDP-glucose. Results In cell extracts no activity was detected. Therefore, cells were permeabilized with 0.3 % (v/v) Triton X-100. After washing away all low molecular weight metabolites, the permeabilized cells were directly used as whole cell biocatalyst. Substrates were 5 mM UDP-glucose and 10 mM NAD(+). Divalent cations were not added to the reaction medium as they promoted UDP-glucose hydrolysis. With this reaction system 5 mM UDP-glucose were converted into 5 mM UDP-glucuronic acid within 3 h. Conclusions Recombinant permeabilized cells of S. pombe can be used to synthesize UDP-glucuronic acid with 100 % yield and selectivity.
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