Article
Biochemistry & Molecular Biology
Cody R. Fisher, Mara C. Ebeling, Zhaohui Geng, Rebecca J. Kapphahn, Heidi Roehrich, Sandra R. Montezuma, James R. Dutton, Deborah A. Ferrington
Summary: This study evaluated the treatment response of haRPE and iPSC-RPE to oxidative stress and potential therapeutics for mitochondrial defects. The results showed disease-specific responses in haRPE, while iPSC-RPE had similar responses regardless of disease status. Analysis of cells generated from the same donor showed a similar response for donors without AMD, but significant differences in treatment response between cell models from AMD donors.
Review
Biochemistry & Molecular Biology
Juha M. T. Hyttinen, Janusz Blasiak, Kai Kaarniranta
Summary: Age-related macular degeneration (AMD) is a complex disease characterized by damage to the retina and is a major cause of vision loss in elderly people. This review explores the role of non-coding RNAs in regulating mitochondrial function and antioxidant stress response in AMD. These molecules have the potential to be used as diagnostic markers and therapeutic targets for AMD and other oxidative stress-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Sophie Tieu, Armen Charchoglyan, Lauryn Paulsen, Lauri C. Wagter-Lesperance, Umesh K. Shandilya, Byram W. Bridle, Bonnie A. Mallard, Niel A. Karrow
Summary: N-acetylcysteine (NAC) is a therapeutic drug with multiple beneficial effects for treating various diseases. It possesses antioxidant, cytoprotective, anti-inflammatory, antimicrobial, and mucolytic properties. However, clinical trials have limitations and conflicting results regarding its effectiveness.
Article
Immunology
Izabella P. Safe, Eduardo P. Amaral, Mariana Araujo-Pereira, Marcus V. G. Lacerda, Vitoria S. Printes, Alexandra B. Souza, Francisco Beraldi-Magalhaes, Wuelton M. Monteiro, Vanderson S. Sampaio, Beatriz Barreto-Duarte, Alice M. S. Andrade, Renata Spener-Gomes, Allyson Guimaraes Costa, Marcelo Cordeiro-Santos, Bruno B. Andrade
Summary: NAC as a precursor of GSH can improve antioxidant status and reduce lipid peroxidation during TB treatment, with minimal impact on cytokine concentrations.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lara Macchioni, Davide Chiasserini, Letizia Mezzasoma, Magdalena Davidescu, Pier Luigi Orvietani, Katia Fettucciari, Leonardo Salviati, Barbara Cellini, Ilaria Bellezza
Summary: Age-related retinal degenerations, such as AMD, are linked to the loss of RPE cells and photoreceptors, involving oxidative stress and inflammatory responses. Long-term oxidative insult can induce senescence in RPE cells, affecting antioxidant response and mitochondrial functionality.
Article
Biochemistry & Molecular Biology
Ivona Bucan, Jelena Skunca Herman, Iris Jeroncic Tomic, Olga Gornik, Zoran Vatavuk, Kajo Bucan, Gordan Lauc, Ozren Polasek
Summary: The pathogenesis of age-related macular degeneration (AMD) remains unclear. This study aimed to investigate the changes in plasma and IgG-specific N-glycosylation across different stages of AMD. The analysis of 2835 subjects revealed interesting differences among the groups, suggesting that N-glycans can serve as biomarkers for AMD. The study also emphasized the need for refined phenotypes and disease severity stages in the analysis of complex diseases.
Article
Oncology
Elena Obrador, Rosario Salvador-Palmer, Rafael Lopez-Blanch, Maria Oriol-Caballo, Paz Moreno-Murciano, Jose M. Estrela
Summary: Malignant melanoma, a cancer derived from melanocytes, can develop on skin, mucous membranes, and other locations. N-acetylcysteine (NAC), an antioxidant drug, may play a role in preventing cancer, but high doses could potentially cause metastatic spread of melanoma. Antioxidant properties of NAC and other promoters of glutathione synthesis should be used cautiously in cancer patients to avoid promoting metastatic growth.
Review
Biochemistry & Molecular Biology
Laura Garcia-Quintanilla, Lorena Rodriguez-Martinez, Enrique Bandin-Vilar, Maria Gil-Martinez, Miguel Gonzalez-Barcia, Cristina Mondelo-Garcia, Anxo Fernandez-Ferreiro, Jesus Mateos
Summary: Age-related macular degeneration (AMD) is a common eye disease in the elderly population, and proteomics-based approaches are powerful tools for studying the molecular mechanisms of its progression and identifying new therapeutic targets and biomarkers for monitoring. Key factors related to the onset and progression of AMD have been identified, but further understanding of the underlying disease mechanisms is needed for next-generation therapies for late-stage forms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Ryo Terao, Tazbir Ahmed, Ayana Suzumura, Hiroko Terasaki
Summary: Aging leads to a decline in organ function, including the eyes, resulting in age-related ocular diseases. Cellular senescence, characterized by cell cycle arrest, plays a significant role in aging and age-related diseases by altering cellular function and secretory phenotypes. Oxidative stress triggers cellular senescence and age-related diseases by inducing senescence markers through reactive oxygen species and mitochondrial dysfunction.
Article
Chemistry, Medicinal
Yamunna Paramaswaran, Aswinprakash Subramanian, Nallupillai Paramakrishnan, Muthusamy Ramesh, Arunachalam Muthuraman
Summary: Diabetic retinopathy (DR) is a retinal degeneration caused by microvascular dysfunction. This study investigates the use of beta-carotene (PBC) from palm oil mill effluent in the treatment of diabetes in mice. The results show that PBC and dexamethasone administration can ameliorate the diabetes-induced retinal alterations.
Article
Biochemistry & Molecular Biology
Anongporn Kobroob, Wachirasek Peerapanyasut, Sirinart Kumfu, Nipon Chattipakorn, Orawan Wongmekiat
Summary: This study demonstrates the efficacy of N-acetylcysteine (NAC) in alleviating renal deterioration caused by long-term BPA exposure, primarily through modulation of signaling proteins in the AMPK-SIRT3-SOD2 axis.
Review
Biochemistry & Molecular Biology
Valentina Bilbao-Malave, Jorge Gonzalez-Zamora, Miriam de la Puente, Sergio Recalde, Patricia Fernandez-Robredo, Maria Hernandez, Alfredo Garcia Layana, Manuel Saenz de Viteri
Summary: AMD is the main cause of legal blindness in developed countries, with a combination of genetic and environmental factors contributing to the increased risk of vision loss. Oxidative stress plays a central role in the pathophysiology of AMD, along with mitochondrial dysfunction and endoplasmic reticulum stress. While treatment with vascular endothelium growth factor inhibitors has decreased the risk of blindness in patients with the exudative form of AMD, research on new therapeutic options to prevent the loss of photoreceptors and retinal pigment epithelium cells characteristic of late stage AMD continues.
Article
Nutrition & Dietetics
Malgorzata Mrowicka, Jerzy Mrowicki, Ewa Kucharska, Ireneusz Majsterek
Summary: Lutein and zeaxanthin, as xanthophyll carotenoids, play a vital role in the human body, especially in protecting the eyes. Consuming foods rich in these compounds can help prevent eye diseases.
Article
Biochemistry & Molecular Biology
Behnaz Shafie, Jalal Pourahmad, Mohsen Rezaei
Summary: This study evaluated the protective effects of ellagic acid and N-acetylcysteine (NAC) against acrolein-induced mitochondrial toxicity in rat liver. NAC effectively reduced ROS, lipid peroxidation, and mitochondrial membrane potential while improving GSH content and complex II activity. However, ellagic acid, despite its antioxidant properties, did not prevent mitochondria from being affected by acrolein as observed in this in vitro study.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Kazutoshi Yamamoto, Ana Opina, Deepak Sail, Burchelle Blackman, Keita Saito, Jeffrey R. Brender, Ronja M. Malinowski, Tomohiro Seki, Nobu Oshima, Daniel R. Crooks, Shun Kishimoto, Yu Saida, Yasunori Otowa, Peter L. Choyke, Jan H. Ardenkjaer-Larsen, James B. Mitchell, W. Marston Linehan, Rolf E. Swenson, Murali C. Krishna
Summary: NAC, as a novel hyperpolarized C-13 MRI probe, shows potential as a non-invasive biomarker for monitoring in vivo redox status, and could be translatable to clinical diagnosis. Its ability to monitor glutathione redox chemistry makes it valuable in metabolic research and therapy development.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Ruvanthi N. Kularatne, Katherine E. Washington, Chandima Bulumulla, Erika L. Calubaquib, Michael C. Biewer, David Oupicky, Mihaela C. Stefan
Article
Polymer Science
Chandima Bulumulla, Ruvanthi N. Kularatne, Ruwan Gunawardhana, Hien Q. Nguyen, Gregory T. McCandless, Michael C. Biewer, Mihaela C. Stefan
Article
Materials Science, Multidisciplinary
Katherine E. Washington, Jia Du, Ruvanthi N. Kularatne, Erika L. Calubaquib, Pooneh Soltantabar, Michael C. Biewer, Mihaela C. Stefan
Article
Chemistry, Multidisciplinary
Ruwan Gunawardhana, Chandima Bulumulla, Prabhath L. Gamage, Adam J. Timmerman, Chinthaka M. Udamulle, Michael C. Biewer, Mihaela C. Stefan
Article
Ophthalmology
Chandima Bulumulla, Ruvanthi N. Kularatne, Timothy Catchpole, Alison Takacs, Abigail Christie, Alexa Gilfoyle, Timothy D. Nguyen, Mihaela C. Stefan, Karl G. Csaky
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2020)
Review
Nanoscience & Nanotechnology
Chandima Bulumulla, Ruwan Gunawardhana, Prabhath L. Gamage, Justin T. Miller, Ruvanthi N. Kularatne, Michael C. Biewer, Mihaela C. Stefan
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Materials Science, Multidisciplinary
Prabhath L. Gamage, Ruwan Gunawardhana, Chandima Bulumulla, Ruvanthi N. Kularatne, Chinthaka M. Udamulle Gedara, Ziyuan Ma, Michael C. Biewer, Mihaela C. Stefan
Summary: This study reported a novel polythiophene material, PDCTVT, constructed by copolymerization of ester-functionalized bithiophene and thiophene vinylene thiophene monomers. The polymer exhibited moderate hole mobilities in organic field-effect transistors and a high on-to-off ratio.
Article
Biology
Chandima Bulumulla, Andrew T. Krasley, Ben Cristofori-Armstrong, William C. Valinsky, Deepika Walpita, David Ackerman, David E. Clapham, Abraham G. Beyene, Jun Ding
Summary: Chemical neurotransmission is a fundamental way for neurons to communicate. However, studying the release of neurotransmitters at the spatial and temporal scales relevant to neuron function has been challenging. In this study, researchers developed a new nanofilm technology that allows real-time visualization of dopamine release and diffusion. They found that dopamine is released from specific hotspots in dendritic processes, and these hotspots co-localize with Bassoon, suggesting a role for Bassoon in organizing active zones in dendrites.
Review
Chemistry, Medicinal
Ruvanthi N. Kularatne, Rachael M. Crist, Stephan T. Stern
Summary: The current state of the art for therapeutic nucleic acid delivery is lipid nanoparticles (LNP), which primarily accumulate in the liver but also have the potential for targeting other organs and cells. This review focuses on the development of next generation tissue-targeted LNP constructs for therapeutic nucleic acids.
Review
Polymer Science
Hanghang Wang, John Michael O. Cue, Erika L. Calubaquib, Ruvanthi N. Kularatne, Somayeh Taslimy, Justin T. Miller, Mihaela C. Stefan
Summary: This review explores the stereospecific polymerization of dienes, vinyl monomers, and epsilon-caprolactone using various neodymium catalysts.
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)