Article
Food Science & Technology
Wenxiu Zhu, Jingzhu Gao, Hongli Liu, Jiaxiu Liu, Tong Jin, Ningbo Qin, Xiaomeng Ren, Xiaodong Xia
Summary: Sodium butyrate exhibits antibiofilm effects against V. parahaemolyticus by inhibiting biofilm formation, disrupting cellular metabolism, reducing cell viability within the biofilm, decreasing bacterial motility and surface hydrophobicity, and interfering with quorum sensing. Additionally, sodium butyrate down-regulates genes associated with biofilm formation in V. parahaemolyticus, suggesting its potential as a potent agent in combating biofilm in food industries.
Article
Biotechnology & Applied Microbiology
Qiuxia Fan, Yahong Yuan, Ting Zhang, Wei Song, Qinglin Sheng, Tianli Yue
Summary: This study investigated the inhibitory effects of Lactobionic acid (LBA) on Vibrio parahaemolyticus planktonic cells and biofilms. The results showed that LBA damaged the cell membranes and downregulated biofilm-related genes, making it a potential low-cost agent for controlling Vibrio parahaemolyticus.
Article
Food Science & Technology
Wenxiu Zhu, Jiaxiu Liu, Yue Zou, Shugang Li, Dongyun Zhao, Haisong Wang, Xiaodong Xia
Summary: This study found that laurel essential oil (LEO) can effectively reduce biofilm formation of Vibrio parahaemolyticus, and it affects its metabolic activity, biofilm structure, and attachment ability. Therefore, it has potential applications in the food industry.
Article
Food Science & Technology
Hongli Liu, Wenxiu Zhu, Yu Cao, Jingzhu Gao, Tong Jin, Ningbo Qin, Xiaodong Xia
Summary: Punicalagin showed significant anti-biofilm activity at sub-inhibitory concentrations, decreasing bacterial motility and metabolic activity in biofilms. It also inhibited EPS production, reduced exopolysaccharides in biofilms, and decreased Raman peak intensities of biofilm components. Punicalagin significantly inhibited V. parahaemolyticus adhesion to Caco-2 cells and downregulated genes related to biofilm formation and virulence.
Article
Biochemical Research Methods
Corrie R. Belanger, Robert E. W. Hancock
Summary: This study presents a protocol for testing physiologically relevant infection conditions in minimal inhibitory concentration (MIC) assays, which can be used to assess the susceptibility of bacterial isolates and novel antimicrobial drugs under conditions more similar to clinical infections. The protocol can be applied to most physiologically relevant liquid medium and aerobic pathogens, taking 3-4 days to complete, and may provide a more accurate assessment of the bactericidal effects of new drugs.
Article
Microbiology
Wenbin Wang, Jianxin Liu, Shanshan Guo, Lei Liu, Qianyun Yuan, Lei Guo, Saikun Pan
Summary: The study identified 101 outer membrane proteins (OMPs) from V. parahaemolyticus as potential candidates for multivalent vaccines and found 19 OMPs relatively specific to V. parahaemolyticus. Surface proteomic study showed that some OMPs have better availability on the cell surface.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
I-Ting Lin, Edan R. Tulman, Steve J. Geary, Xiaohui Zhou
Summary: The study reveals that the interaction between VgpA and VscN2 is crucial for the function of T3SS2 in Vibrio parahaemolyticus, affecting the secretion of substrates and the distribution of T3SS2 proteins within bacterial cells.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Microbiology
Miaomiao Zhang, Liyan Cai, Xi Luo, Xue Li, Tingting Zhang, Fei Wu, Yiquan Zhang, Renfei Lu
Summary: In this study, the effects of sublethal dose of chloramphenicol on Vibrio parahaemolyticus were investigated using various phenotypic assays and RNA sequencing. The results showed that the growth rate, biofilm formation, c-di-GMP synthesis, motility, cytoxicity, and adherence activity of V. parahaemolyticus were significantly decreased by the sublethal dose of chloramphenicol. RNA-seq analysis revealed differential expression of genes related to antibiotic resistance, virulence, biofilm formation, and regulation. The findings suggest that chloramphenicol has a significant impact on the physiology of V. parahaemolyticus.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Jianan Liu, Ke Zhang, Hongyan Wu, Junya Zhu, Hongshun Hao, Jingran Bi, Hongman Hou, Gongliang Zhang
Summary: This study investigated the antibacterial effects of benzyl isothiocyanate (BITC) against Vibrio parahaemolyticus at the protein level. It was found that BITC could downregulate the expression of certain outer membrane proteins, leading to a reduction in biofilm production and loss of outer membrane integrity, thus inhibiting the growth of V. parahaemolyticus.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Wenting Liu, Lulu Hao, Hai Xia, Huajuan Wang, Wenfu Hou, Hongxun Wang, Min Zhou
Summary: In this study, two genetically related bacteriophages, F23s2 and H256D1, were screened and found to have a good inhibitory effect on multidrug-resistant V. parahaemolyticus strains in seafood. They showed a broad host spectrum and high stability, and could potentially be applied to control V. parahaemolyticus contamination worldwide.
FOODBORNE PATHOGENS AND DISEASE
(2023)
Article
Environmental Sciences
Peyman Namadi, Zhiqiang Deng
Summary: This study develops an ensemble model for nowcasting the level of Vibrio parahaemolyticus (V.p) in oysters in the marine environment, using genetic programming and deep neural networks. The model is effective in predicting V.p levels in different regions, demonstrating the impact of environmental conditions on accuracy. This approach can be used as an effective tool for risk mitigation.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2023)
Article
Food Science & Technology
Seul-Ki Park, Kyung-Jin Cho, Du-Min Jo, Young-Mog Kim
Summary: This study presents a new approach for detecting pathogenic Vibrio spp. in contaminated seafood, which combines PCR with a simplified DNA extraction process and pre-culturing step. The method has been validated and shown to be efficient, time-saving, and highly sensitive, offering a potential alternative for improving safety measures in the food industry.
Article
Veterinary Sciences
Germain Maurus, Thi Hang Ho, Po -Tsang Lee
Summary: This study used Scutellaria baicalensis extract (SBE) to enhance the immune response and resistance of shrimps to Vibrio parahaemolyticus. SBE obtained through solid-liquid extraction (SLE) showed stronger antibacterial activity compared to extracts obtained through pressurized liquid extraction (PLE). In vitro experiments demonstrated that SBE (SLE) stimulated a stronger immune response and exhibited better bactericidal activity. Therefore, SBE (SLE) was chosen for in vivo feeding trial.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Fisheries
Novi Rosmala Dewi, Huai-Ting Huang, Yu-Sheng Wu, Zhen-Hao Liao, Yu-Ju Lin, Po-Tsang Lee, Fan-Hua Nan
Summary: The study found that guava leaf extract (GLE) can enhance immune responses, growth performance, and reduce infection of Vibrio parahaemolyticus in white shrimp.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Immunology
Wenfu Hou, Tingting Liu, Yi Zhang, Wensi Wang, Yang Yi, Limei Wang, Hongxun Wang
Summary: The antibacterial mechanism of epsilon-poly-lysine against Vibrio parahaemolyticus was investigated using label-free proteomic analysis. The study found that epsilon-PL treatment led to down-regulation of proteins involved in bacterial chemotaxis and RNA transport, resulting in decreased bacterial adaptability. This highlights Che R and Che V as critical action sites of e-PL in inactivating V. parahaemolyticus.
MICROBIAL PATHOGENESIS
(2021)
Article
Biotechnology & Applied Microbiology
Yen-Te Liao, Valerie M. Lavenburg, Marion Lennon, Alexandra Salvador, Angeline L. Hsu, Vivian C. H. Wu
Summary: This study investigated the prevalence of bacteriophages specific to Shiga toxin-producing Escherichia coli (STEC) strains in an organic farm and how it is influenced by environmental factors. The findings suggest that the presence of animals in the farm contributes to the prevalence of STEC-infecting phages in the surrounding environment, and their presence is negatively correlated with their STEC hosts.
JOURNAL OF FOOD SAFETY
(2023)
Article
Food Science & Technology
Hui-Erh Chai, Cheng-An Hwang, Lihan Huang, Vivian C. H. Wu, Lee-Yan Sheen
Summary: Almonds and black peppercorns contaminated with Salmonella, STEC, and Listeria monocytogenes were successfully decontaminated using gaseous chlorine dioxide. The study identified the concentrations of ClO2 and exposure durations required to achieve significant reductions in pathogen populations, which can be applied in pilot-scale or larger decontamination processes for almonds and black peppercorns.
Article
Biotechnology & Applied Microbiology
Jing Ni Tan, Cheng-An Hwang, Lihan Huang, Vivian C. H. Wu, Hsin- Hsiao
Summary: The study aims to identify effective treatment parameters for chlorine dioxide gas (ClO2(g)) for commercial-scale decontamination of different types of produce. The results show that the treatment has different effects on the reduction of pathogens on various fruits and vegetables under different concentrations and humidity levels.
JOURNAL OF FOOD SAFETY
(2023)
Article
Biotechnology & Applied Microbiology
Bhargavi Rane, Alison Lacombe, Jiewen Guan, David F. Bridges, Shyam Sablani, Juming Tang, Vivian C. H. Wu
Summary: This study investigated the effect of gaseous chlorine dioxide on reducing microbial contamination on black peppercorns. The results showed that gaseous chlorine dioxide can effectively reduce the levels of coliforms, aerobic bacteria, yeasts, and molds without affecting the visual quality of black peppercorns.
JOURNAL OF FOOD SAFETY
(2023)
Article
Agriculture, Multidisciplinary
Laiwang Hou, Darong Li, Ni Zhang, Jiayi Zhao, Yong Zhao, Xiaohong Sun
Summary: The study aimed to establish a recombinase-aided amplification (RAA) method combined with lateral flow dipstick (LFD) for rapid detection of Klebsiella pneumoniae. The optimized RAA-LFD method showed high specificity to K. pneumoniae with no cross-reaction with other pathogens. The detection limit of RAA-LFD was higher than PCR detection, and it could detect K. pneumoniae at low initial concentrations in artificially spiked milk samples. The RAA-LFD assay demonstrated to be an accurate and sensitive detection method for K. pneumoniae.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Chemistry, Applied
Bhargavi Rane, Alison Lacombe, Jiewen Guan, Lillian Lucero, David F. Bridges, Shyam Sablani, Juming Tang, Vivian C. H. Wu
Summary: This study found that fumigation with gaseous chlorine dioxide (ClO2) can effectively reduce mold contamination and toxin levels in almonds. At high concentrations, gaseous ClO2 demonstrated an 84.4% degradation efficiency of aflatoxin B1 (AFB1) and significantly reduced the number of Aspergillus flavus on almond kernels. The treated almonds also showed continued suppression of AFB1 even after one-month storage at low temperature, without any significant oxidative effects or color changes.
Article
Biotechnology & Applied Microbiology
Darong Li, Jiayi Zhao, Weiqing Lan, Yong Zhao, Xiaohong Sun
Summary: Vibrio parahaemolyticus has become a significant threat to human health, and the development of rapid and accurate diagnostics is crucial for monitoring its outbreak and spread. In this study, we developed a novel assay, RAA-LFD, which combines recombinase aided amplification (RAA) with lateral flow dipstick (LFD) for the detection of V. parahaemolyticus. RAA-LFD showed excellent specificity and could detect very low levels of V. parahaemolyticus in genomic DNA and spiked food samples. However, the sensitivity of the assay was affected by the presence of food matrix, reducing the detection limit by 10 to 100 times. In field samples, RAA-LFD showed good agreement with the GB4789.7-2013 method and PCR method. Overall, RAA-LFD is a highly accurate and sensitive tool for point-of-care diagnosis of V. parahaemolyticus, meeting the increasing demand for rapid diagnostics.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Infectious Diseases
Tengteng Li, Hong Chen, Jiayi Zhao, Zhenxiang Tao, Weiqing Lan, Yong Zhao, Xiaohong Sun
Summary: In this study, a novel Salmonella phage vB_SalM_SPJ41 was isolated from poultry farms in Shanghai, China. It was found that this phage could lyse multiple serotypes of antibiotic-resistant S. enterica and effectively inhibit the biofilm produced by antibiotic-resistant S. enterica. Additionally, the phage showed promising results in reducing the levels of S. Enteritidis and S. Derby in lettuce and salmon at low temperatures. Genomic analysis revealed the absence of virulence factor genes or antibiotic resistance genes in the phage.
Article
Food Science & Technology
Jiewen Guan, Alison Lacombe, Bhargavi Rane, Juming Tang, Shyam Sablani, Vivian C. H. Wu
Summary: Limited information on the effectiveness of gaseous chlorine dioxide (ClO2) treatments against Listeria and spoilage microorganisms (MOs) on fresh apples under controlled atmosphere (CA) conditions is available. This study found that gaseous ClO2 combined with CA storage effectively inhibited Listeria innocua and reduced spoilage microorganisms on fresh apples. The treatment with gaseous ClO2 did not significantly affect the quality of the apples during storage, except for lenticel breakdown observed on higher concentration treated apples after 28 days.
Article
Microbiology
Changrong Chen, Zhenxiang Tao, Tengteng Li, Hong Chen, Yong Zhao, Xiaohong Sun
Summary: The detection rate of Klebsiella pneumoniae in food is increasing and it has become a food pathogen. This poses a threat to global health due to the emergence of multidrug-resistant (MDR) and hypervirulent (hv) K. pneumoniae. Phage vB_KpP_HS106, isolated from hospital sewage, shows promise as an antibacterial agent against MDR-hv K. pneumoniae strains.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Chemistry, Applied
Xin Yang, Weiqing Lan, Xiaohong Sun
Summary: Chitosan, as a bio-renewable natural polymeric material, can be enhanced in antibacterial and antioxidant activity through grafting with phenolic acids, thus expanding its application in food preservation. This review focuses on evaluating the structure, antimicrobial and antioxidant activities, and cytotoxicity of phenolic acid-g-chitosan, as well as its application in various food products. The results show that phenolic acid-g-chitosan exhibits enhanced antibacterial and antioxidant activities compared to chitosan, without cytotoxicity. It has been successfully applied in various food products such as fruits, vegetables, and meat. This review provides a valuable reference for rational design and further development of phenolic acid-g-chitosan in food preservation.
Article
Biochemistry & Molecular Biology
Weiqing Lan, Jiaxin Zhao, Xiaoyi Wei, Yuqing Sun, Shucheng Liu, Xiaohong Sun
Summary: This study investigated the impact of chitosan-grafted-caffeic acid (CS-g-CA) and ultrasound (US) on myofibrillar proteins (MPs) in pompano (Trachinotus ovatus) during 24 days of ice storage. The results demonstrated that the synergistic effects of US and CS-g-CA effectively delayed the protein oxidation and degradation, maintaining the structure and sensory properties of the fish. The findings are valuable for the quality maintenance of marine fish.
Article
Biotechnology & Applied Microbiology
Xin Yang, Weiqing Lan, Xiaohong Sun
Summary: The study found that ultrasound-assisted chlorogenic acid grafted chitosan (GUA) soaking of sea bass fillets can reduce protein oxidation and degradation, stabilize the tertiary structure of proteins, and improve the microstructure and texture properties of gels.
JOURNAL OF FOOD SAFETY
(2023)
Article
Microbiology
Yujie Zhang, Ai Kitazumi, Yen-Te Liao, Benildo G. de los Reyes, Vivian C. H. Wu
Summary: Agricultural microbiomes serve as reservoirs of antibiotic resistance genes and bacteriophages play a significant role in the horizontal transfer of these genes. Through studying the diversity of bacterial and viral microbiota in fecal and environmental samples from an organic farm, it was found that bacteriophages belonging to the order Caudovirales, particularly in the Siphoviridae family, contained diverse antibiotic resistance gene types and interacted with various bacterial hosts, highlighting their important role in ARG transmission.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Yen-Te Liao, Yujie Zhang, Alexandra Salvador, Leslie A. Harden, Vivian C. H. Wu
Summary: The application of lytic bacteriophages can be a promising alternative intervention technology in the food industry to relieve antibiotic resistance pressure and control bacterial pathogens. However, the information on phage application to mitigate pathogens on sprouts is limited. This study characterized a T4-like Escherichia phage Sa45lw for its biocontrol potential against STEC O45 on mung bean seeds, and found that the phage effectively reduced the bacterial population.
MICROBIOLOGY SPECTRUM
(2022)
Article
Food Science & Technology
Ewelina Bigoraj, Iwona Kozyra, Agnieszka Kaupke, Zbigniew Osinski, James Lowther, Artur Rzezutka
Summary: The aim of this study was to assess the prevalence of human enteric viruses in mussels sold on the Polish market and study the fecal contamination of shellfish. The study found that human enteric viruses were commonly present in shellfish, with norovirus being the most frequently detected. The study also observed a seasonal distribution pattern of viruses in shellfish samples.
Editorial Material
Food Science & Technology
Bojan Blagojevic, Lis Alban Denmark
Article
Food Science & Technology
Indy Magnus, Fatemeh Abbasi, Hugo Thienpont, Lien Smeesters
Summary: Food safety and quality are increasingly important in our society, driving the development of novel optical food sensing technologies. However, current optical sensing technologies are often limited in their sensitivity and product variability. In this study, we developed a novel pistachio nut screening methodology using fluorescence spectroscopy and advanced chemometrics to simultaneously detect shells, tree parts, and aflatoxins. By optimizing hardware and software parameters, we achieved a high classification accuracy for healthy pistachio kernels.
Article
Food Science & Technology
Xiaodong Wang, Jingqiang Yu, Haixia Wu, Leiying Wu, Wuyunga Borjihan, Cong Wang, Munkhjargal Burenjargal, Alideertu Dong
Summary: This study developed a novel photon-phonon coupling (PPC) cold sterilization technology and compared its effects with low-temperature pasteurization and microwave ultraviolet. The results showed that PPC effectively reduced bacterial count in raw milk with minimal impact on milk quality and nutritional composition.
Review
Food Science & Technology
Riyadh Abdulmalek Hassan, Sharina Abu Hanifah, Lee Yook Heng, Fawaz Al-badaii, Alizar Ulianas
Summary: This study used bibliometric visualisation map analysis to explore the publication outputs, trends, and hotspots for endocrine disruptors (EDMs) biosensor development in relation to mycotoxins. The analysis showed that the current literature primarily focuses on developing biosensors for detecting highly toxic and widely distributed mycotoxins in food, but there needs to be more focus on biosensors for detecting mycotoxins known as endocrine disruptors.
Article
Food Science & Technology
Yajie Zheng, Jianpeng Dou, Changpo Sun, Hujun Liu, Shipei Zhang, Wenfu Wu
Summary: This study investigated the efficiency of modified SA/ll-glucan/Mt microsphere adsorbents in removing DON and ZEN from CSL. The microspheres showed high adsorption capacity, and simple and rapid regeneration was achieved without affecting the removal efficiency of mycotoxins from CSL.
Article
Food Science & Technology
Carlos Adelantado, Jose Angel Salatti-Dorado, Carlos Herrera, Lourdes Arce, Maria Jose Cardador
Summary: High-performance-ion mobility spectrometry (HPIMS) was used for the first time in the analysis of olive oil samples, following a simple liquid-liquid extraction procedure. The developed methodology is user-friendly, fast, and inexpensive, complying with the principles of green chemistry. The proposed approach, combined with multivariate analysis, showed potential in classifying olive oil samples based on their organoleptic quality, with high accuracy rates.
Article
Food Science & Technology
Jia-Wen Wei, Jia-Rong He, Shi-Yi Chen, Yi-Han Guo, Xuan-Zhu Huo, Nuan Zheng, Shuo-Hui Cao, Yao-Qun Li
Summary: Polycyclic aromatic hydrocarbons (PAHs) are a major cause of human cancer. This study developed a quantitative analysis method for PAH4 using the back propagation neural network (BPNN) algorithm and constant wavelength synchronous fluorescence (CWSF) spectra as the data sets. The method can predict the concentrations of PAH4 in edible oil samples without preprocessing or pre-separation. It has been proven to be a powerful tool for the rapid detection of PAH4.
Article
Food Science & Technology
Liyun Chen, Xiumei Li, Jingyi Chen, Ranxun Lin, Yuhan Mai, Yuxin Lin, Guodong Wang, Zheng Chen, Wei Zhang, Jiang Wang, Cai Yuan, Longguang Jiang, Peng Xu, Mingdong Huang
Summary: Food spoilage is primarily caused by microbial contamination. Curcumin has shown moderate antimicrobial activity as a food preservative. This study formulates curcumin with zinc acetate to enhance its efficacy, resulting in Cur-Zn with increased antimicrobial activity against multiple food-contaminating bacteria and fungi. With light illumination, the antimicrobial activity of Cur-Zn is further enhanced due to the photodynamic inactivation (PDI) effect.
Article
Food Science & Technology
Marta Pilar Ortiz-Moriano, Gonzalo Machado-Schiaffino, Eva Garcia-Vazquez, Alba Ardura
Summary: Mislabelling in the global fisheries sector raises concerns about the identity, safety, and sustainability of seafood products. This study examined mislabelling in shrimps and prawns sold in the Spanish market and assessed potential risks of heavy metal ingestion.
Article
Food Science & Technology
Doyeon Kim, Jinwoo Kim, Minsik Kim
Summary: This study demonstrates the enhanced bactericidal activity of engineered lysins fused with amphipathic antimicrobial peptides against Gram-negative bacterium Cronobacter sakazakii. The fusion lysins are capable of disrupting bacterial cells and significantly reducing the risk of infection within a certain range of temperature and pH. Additionally, the study also shows the bactericidal effect of these fusion lysins against other Gram-negative pathogens.
Article
Food Science & Technology
Seul-Ki Park, Kyung-Jin Cho, Du-Min Jo, Young-Mog Kim
Summary: This study presents a new approach for detecting pathogenic Vibrio spp. in contaminated seafood, which combines PCR with a simplified DNA extraction process and pre-culturing step. The method has been validated and shown to be efficient, time-saving, and highly sensitive, offering a potential alternative for improving safety measures in the food industry.
Article
Food Science & Technology
Suwimol Surareungchai, Chaleeda Borompichaichartkul, Chitsiri Rachtanapun, Nutthachai Pongprasert, Pongphen Jitareerat, Varit Srilaong
Summary: This study builds upon previous research and focuses on risk assessment and analysis in the organic salad industry. The results show that previous risk matrix models can be adapted for various raw materials, and preventive control plans can be applied across different aspects of the production process.
Article
Food Science & Technology
Xuan Zhang, Changling Fang, Xiaoyi Lou, Feng Han, Siman Li, Yongfu Shi, Dongmei Huang
Summary: This study aimed to assess the levels of Biogenic amines (BAs) in four common food types in the Chinese market. It was found that cheese and seafood derived product showed higher levels of BAs, and the histamine level in mackerel and yellow rice wine exceeded the limits.
Article
Food Science & Technology
Martina Foschi, Alessandra Biancolillo, Federico Marini, Francesco Cosentino, Francesca Di Donato, Angelo Antonio D'Archivio
Summary: This study aimed to develop a potentially non-destructive and routine-compatible method for the geographical traceability of high-valued Italian chickpeas. By analyzing the spectral information of the outer and inner parts of the kernels, it was found that the spectral analysis of the outer part yielded accurate and reliable results, indicating the possibility of non-destructive authentication.